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Cigarette smoke‑induced malignant transformation via STAT3 signalling in pulmonary epithelial cells in a lung‑on‑a‑chip model

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Bio-Design and Manufacturing 2020年第4期3卷 383-395页

作者： Wei Hou Siyi Hu ken‑tye yong Jie Zhang Hanbin Ma CAS Key Laboratory of Bio‑Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesNo.88 Keling RoadHuqiu DistrictSuzhou 215163JiangsuPeople’s Republic of China Department of Respiratory and Critical Care Medicine The Second Hospital of Jilin UniversityNo.218 Ziqiang StreetNanguan DistrictChangchun 130041JilinPeople’s Republic of China School of Electrical and Electronic Engineering Nanyang Technological UniversitySingapore 639798Singapore

Background Chronic obstructive pulmonary disease(COPD)is a severe public health problem.Cigarette smoke(CS)is a risk factor for COPD and lung cancer.The underlying molecular mechanisms of CS-induced malignant transformation of bronchial epithelial cells remain unclear.In this study,we describe a lung-on-a-chip to explore the possible mechanistic link between cigarette smoke extract(CSE)-associated COPD and lung cancer.Methods An in vitro lung-on-a-chip model was used to simulate pulmonary epithelial cells and vascular endothelial cells with CSE.The levels of IL-6 and TNF-αwere tested as indicators of inflammation using an enzyme-linked immune sorbent assay.Apical junction complex mRNA expression was detected with qRT-PCR as the index of epithelial-to-mesenchymal transition(EMT).The effects of CSE on the phosphorylation of signal transduction and transcriptional activator 3(STAT3)were detected by Western blotting.Flow cytometry was performed to investigate the effects of this proto-oncogene on cell cycle distribution.Results Inflammation caused by CSE was achieved in a lung-on-a-chip model with a mimetic movement.CSE exposure induced the degradation of intercellular connections and triggered the EMT process.CSE exposure also activated the phosphorylation of proto-oncogene STAT3,while these effects were inhibited with HJC0152.Conclusions CSE exposure in the lung-on-a-chip model caused activation of STAT3 in epithelial cells and endothelial cells.HJC0152,an inhibitor of activated STAT3,could be a potential treatment for CS-associated COPD and lung cancer.

关键词： Cigarette smoke Microfluidic chips STAT3 Chronic obstructive pulmonary disease

Advanced low-dimensional carbon materials for flexible devices

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InfoMat 2020年第4期2卷 698-714页

作者： Chandreyee M.Das Lixing Kang Qingling Ouyang ken-tye yong School of Electrical and Electronic Engineering Nanyang Technological UniversitySingapore

We live in a digitized era,where we are completely surrounded by a plethora of automated electronic systems,be it a smart home energy controller or a selfoperated diagnostic kiosk in a clinic.With the recent advent of onedimensional(1D)and two-dimensional(2D)nanomaterials like carbon nanotube(CNT)and graphene,the world of electronics has revolutionized with state-of-the-art product paradigms.These nanomaterials possess desirable features of large surface area,excellent electrical conductivity,and high mechanical strength.Electronic devices made out of these materials have the added advantages of being flexible,light-weight,and durable.Thus,presentday devices that utilize these substances as channel or electrode materials have been able to undergo a positive transformation as compared with conventional structures.Flexibility and bendability are some of the coveted aesthetics of modern-day electronics and the use of these 1D and 2D nanomaterials imparts such features to the devices,without having to compromise on key output characteristics like sensitivity and efficiency.In this short review,we discuss about various new configurations that are based on graphene,CNT,and other materials like transition metal dichalcogenides,and how these materials have been able to metamorphose the attributes of conventional devices.

关键词： 1D/2D materials carbon nanotube flexible electronics graphene

Biodegradable nanocarriers for small interfering ribonucleic acid （siRNA） co-delivery strategy increase the chemosensitivity of pancreatic cancer cells to gemcitabine

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Nano Research 2017年第9期10卷 3049-3067页

作者： Chengbin Yang Kok ken Chan Wen-Jen Lin Alana Mauluidy Soehartono Guimiao Lin Huiting Toh Ho Sup Yoon Chih-Kuang Chen ken-tye yong School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798 Singapore Polymeric Biomaterials Lab Department of Fiber and Composite Materials Feng Chia University Taichung 40724 Taiwan China Key Lab of Biomedical Engineering School of Medicine Shenzhen University Shenzhen 518060 China Division of Structural Biology & Biochemistry School of Biological Sciences Nanyang Technological University Singapore 639798Singapore Department of Genetic Engineering College of Life Sciences Kyung Hee University Yongin-si Gyeonggi-do 446701 Republic of Korea

Ribonucleic acid (RNA) interference (RNAi) therapies are promising cancer treatment modalities that can specifically target abnormal proto-oncogenes, thus improving the therapeutic effect. For the treatment of pancreatic cancer, targeting one mutant proto-oncogene by RNAi usually does not yield the desired therapeutic efficiency. Both K-ras gene mutations and Notch1 overexpression are common symptoms in pancreatic cancer patients, and play a crucial role in pancreatic cancer cell drug resistance. In this study, biodegradable charged polyester-based vectors (BCPVs) were synthesized for the co-delivery of K-ras and Notch1 small interfering ribonucleic acid (siRNA) into MiaPaCa-2 cells (pancreatic cancer cell line) to overcome drug resistance to gemcitabine (GEM), a first-line chemotherapeutic drug used in the clinic. BCPVs could effectively absorb negative siRNA to form a capsule-like structure, prevent siRNA from nuclease digestion in the serum, and promote effective siRNA cell internalization and endosomal escape. Through K-ras and Notch1 gene silencing in MiaPaCa-2 cells, BCPV-siRNAK-ras-siRNANotch1 nanocomplexes effectively reversed the epithelia-mesenchymal transition (EMT) in MiaPaCa-2 cells, thereby greatly enhancing the sensitivity of MiaPaCa-2 cells to GEM. MiaPaCa-2 cell proliferation, migration, and invasion were effectively inhibited, and cell apoptosis was also significantly enhanced by the synergistic antitumor effect of BCPV-siRNAK-ras-siRNANotch1 nanocomplexes and GEM. These results suggest that this combination RNAi therapy can be used to improve cancer cell sensitivity to chemotherapeutic drugs. Specifically, this newly developed strategy has a great potential for treating pancreatic cancer.

关键词： pancreatic cancer small interfering ribonucleic acid (siRNA) biodegradable charged polyester-based vector (BCPV) gemcitabine (GEM) epithelia-mesenchymal transition (EMT)

Toxicity assessment and long-term three-photon fluorescence imaging of bright aggregation-induced emission nanodots in zebrafish

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Nano Research 2016年第7期9卷 1921-1933页

作者： Dongyu Li Xinyuan Zhao Wei Qin Hequn Zhang Yue Fei Liwei Liu ken-tye yong Guangdi Chen Ben Zhong Tang Jun Qian State Key Laboratory of Modern Optical Instrumentation Centre for Optical and Electromagnetic Research Zhejiang Provincial Key Laboratory for Sensing Technologies Zhejiang University Hangzhou 310058 China Bioelectromagnetics Laboratory Institute of Environmental Health Zhejiang University School of Medicine Hangzhou 310058 China Department of Chemistry The Hang Kong University of Science & Technology Clear Water Bay Kowloon Hong Kong China Changchun University of Science and Technology (CUST) Changchun 130022 China School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798 Singapore

Aggregation-induced emission （AIE） luminogen displays bright fluorescence and has photobleaching resistance in its aggregation state. It is an ideal fluorescent contrast agent for bioimaging. Multiphoton microscopy is an important tool for bioimaging since it possesses the ability to penetrate deep into biological tissues. Herein, we used AIE luminogen together with multiphoton microscopy for long-term imaging of zebrafish. A typical AIE luminogen, 2,3-bis（4-（phenyl（4- （1,Z2-triphenylvinyl） phenyl）amino）phenyl） fumaronitrile （TPE-TPA-FN or TTF）, was encapsulated with 1,2-distearoyl-sn-glycero-3-phosphoethanola-mine-N- [methoxy（polyethylene glycol）-2000] （DSPE-mPEG2000） to form nanodots that exhibited bright three-photon fluorescence under 1,560 nm-femtosecond （fs） laser excitation. The TTF-nanodots were chemically stable in a wide range of pH values and showed no in vivo toxicity in zebrafish according to a series of biological tests. The TTF-nanodots were microinjected into zebrafish embryos, and the different growth stages of the labeled embryos were monitored with a three-photon fluorescence microscope. TTF-nanodots could be traced inside the zebrafish body for as long as 120 hours. In addition, the TTF-nanodots were utilized to target the blood vessel of zebrafish, and three-photon fluorescence angiogram was performed. More importantly, these nanodots were highly resistant to photobleaching under 1,560 nm-fs excitation, allowing long-term imaging of zebrafish.

关键词： biocompatible organicnanodots,aggregation-inducedemission,three-photon fluorescence,bioimaging,zebrafish

Sustainable wood-based nanotechnologies for photocatalytic degradation of organic contaminants in aquatic environment

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Frontiers of Environmental Science & Engineering 2021年第4期15卷 19-40页

作者： Xinyi Liu Caichao Wan Xianjun Li Song Wei Luyu Zhang Wenyan Tian ken-tye yong Yiqiang Wu Jian Li College of Materials Science and Engineering Central South University of Forestry and TechnologyChangsha410004China Material Science and Engineering College Northeast Forestry UniversityHarbin150040China School of Electrical and Electronic Engineering Nanyang Technological UniversitySingapore639798Singapore Yihua Lifestyle Technology Co. LtdHuaidong Industrial ZoneLianxia TownChenghai DistrictShantou515834China Caichao Wan

Wood-based nanotechnologies have received much attention in the area of photocatalytic degradation of organic contaminants in aquatic environment in recent years,because of the high abundance and renewability of wood as well as the high reaction activity and unique structural features of these materials.Herein,we present a comprehensive review of the current research activities centering on the development of wood-based nanocatalysts for photodegradation of organic pollutants.This review begins with a brief introduction of the development of photocatalysts and hierarchical structure of wood.The review then focuses on strategies of designing novel photocatalysts based on wood or its recombinants(such as 1D fiber,2D films and 3D porous gels)using advanced nanotechnology including sol-gel method,hydrothermal method,magnetron sputtering method,dipping method and so on.Next,we highlight typical approaches that improve the photocatalytic property,including metal element doping,morphology control and semiconductor coupling.Also,the structure-activity relationship of photocatalysts is emphasized.Finally,a brief summary and prospect of wood-derived photocatalysts is provided.

关键词： Wood Nanocatalysts Photodegradation Organic contaminants Composites

Uptake of transferrin-conjugated quantum dots in single living cells

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Chinese Optics Letters 2010年第10期8卷 940-943页

作者：陈丹妮许改霞 Bahi Ahmed Ali ken-tye yong 周翠红王晓梅屈军乐 Paras N.Prasad 牛憨笨 College of Optoelectronic Science and Engineering Huazhong University of Science and Technology Institute of Optoelectronics Key Lab of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province Shenzhen Key Lab of Biomedicine Engineering Shenzhen University College of Medicine Shenzhen University Mubarak City for Scientific Research and Technology Applications School of Electrical and Electronic Engineering Nanyang Technological University Institute for Laser Photonics and Biophotonics State University of New York at Buffalo

We study the uptake and distribution of transferrin （Tf）-conjugated CdSe/CdS/ZnS quantum dots （QDs） in single living HeLa cells with both fluorescence confocal microscopy and three-dimensional （3D） reconstruction technique. By increasing the co-incubation time or the dosage of QDs-Tf, we find that the uptake of QDs-Tf bioconjugates in the cells increases correspondingly, but with different uptake rates. Additionally, the distribution of QDs-Tf, in single live HeLa cells is time dependent. To our knowledge, this is the first study on quantitatively analyzing the uptake and distribution of bioconjugated QDs in single living cells. Such QDs nanoplatform can be further modified for developing biomedical evaluation tool in cancer diagnosis and targeted drug delivery.

关键词： Confocal microscopy Drug delivery Three dimensional

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3.3′二乙基硫醛三碳菁化碘拉曼光谱研究

光谱学与光谱分析 2010年第12期30卷 3244-3248页

作者：蔡红星张宇 ken-tye yong 邵桢李源殉张喜和长春理工大学纳米光子学与生物光子学国际联合研究中心吉林长春130022 纽约州立大学布法罗分校激光、光子学与生物光子学研究所美国纽约14260-4200 长春理工大学计算机科学与技术学院吉林长春130022 吉林大学数学学院吉林长春130012

给出了3.3′二乙基硫醛三碳菁化碘(DTTC)分子的自然拉曼光谱、表面增强拉曼光谱(SERS)、以及理论计算拉曼光谱。SERS谱的增强基底是直径60 nm的金球和50 nm长的金棒,外包附一层硫基聚乙二醇(thiol-polyethylene glycol,简称Mpeg-SH)做... 详细信息

给出了3.3′二乙基硫醛三碳菁化碘(DTTC)分子的自然拉曼光谱、表面增强拉曼光谱(SERS)、以及理论计算拉曼光谱。SERS谱的增强基底是直径60 nm的金球和50 nm长的金棒,外包附一层硫基聚乙二醇(thiol-polyethylene glycol,简称Mpeg-SH)做为稳定层。同时,采用密度泛函(DFT)方法计算了DTTC分子的拉曼光谱,计算基于B3LYP/6-31G基组。经过仔细对比,计算光谱和两种实测光谱特征峰位有很好的一致性。对于不一致的部分,分析原因可能是由于金纳米粒子表面等离子体波对于不同的峰位,其增强程度不同所引起的。这项工作将有利于基于DTTC分子的红外波段拉曼光谱技术在生化领域的应用。

关键词：拉曼光谱表面增强拉曼散射理论计算拉曼光谱 3.3′二乙基硫醛三碳菁化碘

Aggregation-induced emission （ALE） dye loaded polymer nanoparticles for gene silencing in pancreatic cancer and their in vitro and in vivo biocompatibility evaluation

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Nano Research 2015年第5期8卷 1563-1576页

作者： Rui Hu Chengbin Yang Yucheng Wang Guimiao Lin Wei Qin Qingling Ouyang Wing-Cheung Law Quoc Toan Nguyen Ho Sup Yoon Xiaomei Wang ken-tye yong Ben Zhong Tang School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798 Singapore The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering School of Medicine Shenzhen University Shenzhen 518060 China Department of Chemistry The Hang Kong University of Science and Technology Clear Water Bay Kowloon Hang Kong China Department of Industrial and Systems Engineering The Hang Kong Polytechnic University Hung Ham Kowloon Hang Kong China Division of Structural Biology & Biochemistry School of Biological Sciences Nanyang Technological University Singapore 539798 Singapore

Spectral Selectivity of CdTe Cells with Substrate Configuration for Photovoltaic/Thermal Applications

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Journal of Thermal Science 2024年第4期33卷 1542-1553页

作者： CHEN ken HU Kongfu ZHAO Bin CHEN Tao HAO yong PEI Gang Department of Thermal Science and Energy Engineering University of Science and Technology of China Key Laboratory of Solar Thermal Conversion of Anhui Province Hefei National Laboratory for Physical Sciences at Microscale Department of Materials Science and EngineeringUniversity of Science and Technology of China Institute of Engineering Thermophysics Chinese Academy of Sciences University of Chinese Academy of Sciences

Existing photovoltaic cells with high infrared emissivity generate huge radiative heat loss in photovoltaic/thermal applications and degrade the photothermal performance.The purpose of this work is to evaluate the full spectral absorptivity of CdTe cells to find a spectrally selective photovoltaic cell for photovoltaic/thermal applications.To this end,the solar absorptivity and mid-infrared thermal emissivity of CdTe cells were tested by ellipsometry,UV-Vis-NIR spectrophotometer,and Fourier transform infrared spectrometer.The experimental results show that the AM 1.5 solar spectrum weighted absorptivity of the substrate configuration CdTe cell reaches 0.91,and the mid-infrared emissivity is only 0.29,while the superstrate configuration cell emissivity is as high as 0.9.Further research shows that substrate configuration with a transparent conductive layer on top can be flexibly grown on metal foils and has spectral selectivity with high solar absorptivity and low mid-infrared emissivity should be considered in the future for photovoltaic/thermal applications.

关键词： photovoltaic/thermal spectral selectivity substrate configuration CdTe cell ITO