Uptake of transferrin-conjugated quantum dots in single living cells

作     者：陈丹妮 许改霞 Bahi Ahmed Ali Ken-Tye Yong 周翠红 王晓梅 屈军乐 Paras N.Prasad 牛憨笨 

作者机构：College of Optoelectronic Science and Engineering Huazhong University of Science and Technology Institute of Optoelectronics Key Lab of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province Shenzhen Key Lab of Biomedicine Engineering Shenzhen University College of Medicine Shenzhen University Mubarak City for Scientific Research and Technology Applications School of Electrical and Electronic Engineering Nanyang Technological University Institute for Laser Photonics and Biophotonics State University of New York at Buffalo 

出 版 物：《Chinese Optics Letters》 (中国光学快报（英文版）)

年 卷 期：2010年第8卷第10期

页      面：940-943页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China (Nos. 30900335, 60878053, and60627003) the Guangdong Province Science Foundation(No. 2008078) the Natural Science Foundation of Shen-zhen University, and the Start-Up Grant from Nanyang Technological University 

主　　题：Confocal microscopy Drug delivery Three dimensional 

摘      要：We study the uptake and distribution of transferrin （Tf）-conjugated CdSe/CdS/ZnS quantum dots （QDs） in single living HeLa cells with both fluorescence confocal microscopy and three-dimensional （3D） reconstruction technique. By increasing the co-incubation time or the dosage of QDs-Tf, we find that the uptake of QDs-Tf bioconjugates in the cells increases correspondingly, but with different uptake rates. Additionally, the distribution of QDs-Tf, in single live HeLa cells is time dependent. To our knowledge, this is the first study on quantitatively analyzing the uptake and distribution of bioconjugated QDs in single living cells. Such QDs nanoplatform can be further modified for developing biomedical evaluation tool in cancer diagnosis and targeted drug delivery.