Aggregation-induced emission （ALE） dye loaded polymer nanoparticles for gene silencing in pancreatic cancer and their in vitro and in vivo biocompatibility evaluation

作     者：Rui Hu Chengbin Yang Yucheng Wang Guimiao Lin Wei Qin Qingling Ouyang Wing-Cheung Law Quoc Toan Nguyen Ho Sup Yoon Xiaomei Wang Ken-Tye Yong Ben Zhong Tang 

作者机构：School of Electrical and Electronic Engineering Nanyang Technological University Singapore 639798 Singapore The Engineering Lab of Synthetic Biology and the Key Lab of Biomedical Engineering School of Medicine Shenzhen University Shenzhen 518060 China Department of Chemistry The Hang Kong University of Science and Technology Clear Water Bay Kowloon Hang Kong China Department of Industrial and Systems Engineering The Hang Kong Polytechnic University Hung Ham Kowloon Hang Kong China Division of Structural Biology & Biochemistry School of Biological Sciences Nanyang Technological University Singapore 539798 Singapore 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2015年第8卷第5期

页      面：1563-1576页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 080503[工学-材料加工工程] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：This work was supported by the National Natural Science Foundation of China (NSFC) (61107017  81301318)  the Start-up grant (M4080141.040) from Nanyang Technological University  Tier 1 Academic Research Funds (M4010360.040 RG29/10) from Singapore Ministry of Education and partially from the Singapore Ministry of Education under a Tier 2 Research Grant MOE2010-T2-2-010 (4020020.040 ARC2/11) and the grant from the Shenzhen Basic Research Project (JC201005280391A) 

主　　题：aggregation-induced emission polymer nanoparticles gene silencing pancreatic cancer 

摘      要：We have developed aggregation-induced emission （AIE） dye loaded polymer nanoparticles with deep-red emission for siRNA delivery to pancreatic cancer cells. Two US Food and Drug Administration （FDA） approved surfactant polymers, Pluronics F127 and PEGylated phospholipid, were used to prepare the dye-loaded nanoparticle formulations and they can be used as nanovectors for gene silencing of mutant K-ras in pancreatic cancer cells. The successful transfection of siRNA by the developed nanovectors was confirmed by the fluorescent imaging and quantified through flow cytometry. Quantitative real time polymerase chain reaction （PCR） indicates that the expression of the mutant K-ras oncogene from the MiaPaCa-2 pancreatic cancer cells has been successfully suppressed. More importantly, our in vivo toxicity study has revealed that both the nanoparticle formulations are highly biocompatible in BALC/c mice. Overall, our results suggest that the AIE dye-loaded polymer nanoparticle formulations developed here are suitable for gene delivery and have high potential applications in translational medicine research.