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Extracellular vesicles-coupled miRnAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1528-1549页

作者： Rosane Mazzarella karin. c.n.n.beltran.yulia n.c.jas Meriem Hamdi Encina M.Gonzalez Juliano c.da Silveira claudia L.V.Leal D.Rizos Department of Animal Reproduction INIA-CSICMadridSpain Department of Biochemistry and Molecular Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain Department Agrarian Production Technical University of MadridUPMMadridSpain Departamento de ciencias Biologicas Universidad Tecnica Particular de LojaUTPLLojaEcuador Department of Anatomy and Embryology FV-UCMMadridSpain Department of Veterinary Medicine FZEA-USPPirassunungaBrazil

Background Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within EVs in maternal reproductive fluids, such as micro RnAs(miRnAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional *** This study evaluated miRnA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LMGs and sign-aling pathways crucial for early embryo development. A total of 333 miRnAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRnAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRnAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differ-ences in miRnAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRnAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and *** Our study su

关键词： Embryo-maternal interaction Epigenetic Exosomes Mammalian Preimplantation Reproductive fluids

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cluster Analysis of the Roma-BZcAT Blazars

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Research in Astronomy and Astrophysics 2024年第5期24卷 117-139页

作者： Dmitry O.Kudryavtsev yulia V.Sotnikova Vladislav A.Stolyarov Timur V.Mufakharov Valery V.Vlasyuk Margarita L.Khabibullina Alexander G.Mikhailov yulia V.cherepkova Special Astrophysical Observatory of the Russian Academy of Sciences Nizhny Arkhyz 369167Russia Astrophysics Group Cavendish LaboratoryUniversity of CambridgeCambridge CB3 OHEUK Kazan Federal University 18 Kremlyovskaya StKazan 420008Russia

Based on the collected multiwavelength data, namely in the radio(nVSS, FIRST, RATAn-600), IR(WISE),optical(Pan-STARRS), UV(GALEX), and X-ray(ROSAT, Swift-XRT) ranges, we have performed a cluster analysis for the blazars of the Roma-BZcAT catalog. Using two machine learning methods, namely a combination of PcA with k-means clustering and Kohonen's self-organizing maps(SOMs), we have constructed an independent classification of the blazars(five classes) and compared the classes with the known Roma-BZcAT classification(FSRQs, BL Lacs, galaxy-dominated BL Lacs, and blazars of an uncertain type) as well as with the high synchrotron peaked(HSP) blazars from the 3HSP catalog and blazars from the TeVcat catalog. The obtained groups demonstrate concordance with the BL Lac/FSRQ classification along with a continuous character of the change in the properties. The group of HSP blazars stands out against the overall distribution. We examine the characteristics of the five groups and demonstrate distinctions in their spectral energy distribution shapes. The effectiveness of the clustering technique for objective analysis of multiparametric arrays of experimental data is demonstrated.

关键词： methods data analysis-galaxies active-(galaxies:)BL Lacertae objects general

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Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen

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Asian Journal of Andrology 2024年第1期26卷 71-76页

作者： Elizaveta Bragina Tatyana Sorokina Avtandil chogovadze yulia Shevchenko Vyacheslav chernykh Olga Barkhatova Irina Rakovskaya Research centre for Medical Genetics Moscow 115522Russia A.n.Belozersky Institute of Physico-chemical Biology Lomonosov Moscow State University Moscow 119992Russia Reprolab Limited Liability company Moscow 119333Russia n.I.Pirogov Russian national Research Medical University Moscow 117997Russia Federal Government Budgetary Institution"national Research center for Epidemiology and Microbiology named after Honorary Academician n.F.Gamaleya of the Ministry of Health of the Russian Federation" Moscow 123098Russia

Recently discovered microcolonial forms of Mycoplasma hominis(***)and their impact on human spermatogenesis are *** spermatozoa of 125 fertile men(sperm donors;from Reprobank[Reproductive Tissue Bank,Moscow,Russial)and of 93 patients with fertility problems(from the Federal State Budgetary Institution"Research centre for Medical Genetics[RcMG]",Moscow,Russia)were *** colonies of *** and microcolonies were detected by molecular biological methods,culture of bacteria,and transmission electron *** unique structure of microcolonial cells,in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12-14 nm,and the ability of microcolonial cells to attach to spermatozoa are *** patients with lower sperm quality,microcolonies of *** were detected 2.5 times more frequently than classical *** detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility.

关键词： ejaculate fertility Mycoplasma hominis spermatogenesis transmission electron microscopy

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Effects of a cocktail Supplement of Ginkgo Biloba and Acai Extract on cognitive Symptoms of Parkinson’s Disease

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Advances in Parkinson's Disease 2024年第3期13卷 57-72页

作者： yulia Dubrovensky Boston University Boston MA USA

Parkinson’s Disease (PD) is a neurodegenerative disorder characterized by motor and non-motor symptoms, including cognitive impairment. current treatments often involve synthetic drugs with significant side effects and potential for dependency. This study investigates the effects of a natural supplement combination of Ginkgo Biloba and Acai Extract on cognitive symptoms in a 77-year-old male with PD. The participant underwent a three-month supplementation regimen, with cognitive function assessed using the Montreal cognitive Assessment (MocA) test before and after the intervention. The results indicated an improvement in cognitive scores, suggesting that the combination of Ginkgo Biloba and Acai Extract may offer a promising alternative or adjunct to conventional PD treatments. This study highlights the potential of natural supplements in managing PD symptoms and calls for further research with larger sample sizes to confirm these findings. Human data was performed in accordance with the Declaration of Helsinki by the Roxbury District IRB Board (IRB number: IRB00011767).

关键词： Parkinson’s Disease (PD) cognitive Function Ginkgo Biloba Acai Extract neurodegenerative Disorders natural Supplements cognitive Symptoms Montreal cognitive Assessment (MocA) Dopaminergic neurons Antioxidants neuroprotection non-Motor Symptoms Oxidative Stress Polyphenols

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Overcoming the challenges Encountered in the Performance Appraisal of Faculty Members

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Journal of Forensic Science and Medicine 2024年第2期10卷 152-154页

作者： Saurabh RamBihariLal Shrivastava Ulfa Zora yulia Putri Ayunigdyah Abhishek Joshi Deputy Director(Research and Development) Datta Meghe Institute of Higher Education and Research NagpurOff CampusDepartment of Community MedicineDatta Meghe Medical CollegeOff-campus Centre of Datta Meghe Institute of Higher Education and ResearchHingna RoadWanadongriNagpurMaharashtraIndia Department of Medical Education Faculty of MedicinePublic Health and NursingUniversitas Gadjah MadaYogyakartaIndonesia Department of Research and Education Dr.Yap Eye HospitalYogyakartaIndonesia Department of community Medicine Jawaharlal Nehru Medical CollegeDatta Meghe Institute of Higher Education and ResearchSawangiWardhaMaharashtraIndia

Performance appraisal of teaching faculty who are involved in the act of delivery of medical education to prepare future doctors plays a pivotal role in measuring their competency level to train students,assessing the effectiveness in discharging their other roles,and acts as a motivating factor to continuously improvise and augment their knowledge and ***,the ground reality is that in most settings,this task is not acknowledged as an action for motivating a faculty member,but rather just an act of *** the identification of these challenges,we must look for remedial measures to overcome them to streamline and enhance the effectiveness of the performance appraisal *** conclusion,the performance appraisal of teaching faculty is an essential and indispensable ***,the success of performance appraisal,in terms of promoting professional development and organizational success,will depend a lot on the effective redressal of the existing challenges.

关键词： Faculty medical education performance appraisal

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The combined Effect of Lumenato and ceramide in the Protection of collagen Damage Induced by neutrophils in normal Human Dermal Fibroblasts

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Advances in Bioscience and Biotechnology 2024年第2期15卷 140-159页

作者： yulia Solomonov Rachel Levy Immunology and Infectious Diseases Laboratory Department of Clinical Biochemistry and Pharmacology Faculty of Health Sciences Ben-Gurion University of The Negev Beer-Sheva Israel

Introduction: collagen is the primary structural protein fibroblasts produce in the skin’s extracellular matrix. Infiltration of neutrophils into the epidermis and dermis by exposure to UV causes collagen damage and contributes to photoaging. Methods: To study the combined effect of Lumenato and ceramide in preventing collagen-1 damage induced by phagocytes, we used co-cultures of normal human dermal fibroblasts (fibroblasts) and activated human neutrophils. The present study aimed to determine the protective effect of the combination of Lumenato and ceramide on fibroblast collagen-1 damage induced by neutrophils. Results: Lumenato (in the range of 6.5 - 208 μg/ml) or ceramide (in the range of 0.1 - 50 μM) inhibited the production of superoxides and MPO by TnFα-stimulated neutrophils, as well as the production of nO by LPS-stimulated macrophages in a dose-dependent manner. The combinations of Lumenato and ceramide, in low concentrations, caused synergistic prevention of fibroblasts’ collagen-1 damage induced by TnFα-activated neutrophils, detected by fluorescence immunostaining and WB analysis. MPO activity in the supernatants of the co-cultures was also synergistically inhibited. Adding Lumenato or ceramide singly or in combinations in these low concentrations to the fibroblast cultures did not affect the expression of collagen-1. The combinations of Lumenato or ceramide in these concentrations also caused a synergistic inhibition of nO production by activated macrophages. conclusions: The results suggest that combining low concentrations of Lumenato and ceramide results in synergistic protection against fibroblasts’ collagen-1 damage induced by neutrophils, thus indicating their possible potential for enhanced skin health.

关键词： Dermal Fibroblasts neutrophils collagen-1 Lumenato ceramide

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Waxes in asphaltenes of crude oils and wax deposits

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Petroleum Science 2016年第4期13卷 737-745页

作者： yulia M.Ganeeva Tatiana n.Yusupova Gennady V.Romanov A.E. Arbuzov Institute of Organic and Physical chemistry Kazan Scientific Center Russian Academy of Sciences Institute of Geology and Petroleum Technologies Kazan (Volga region) Federal University

composition and molecular mass distribution of n-alkanes in asphaltenes of crude oils of different ages and in wax deposits formed in the borehole equipment were studied. In asphaltenes, n-alkanes from c12 to c60 were detected. The high molecular weight paraffins in asphaltenes would form a crystalline phase with a melting point of 80–90 ℃. The peculiarities of the redistribution of high molecular paraffin hydrocarbons between oil and the corresponding wax deposit were detected. In the oils, the high molecular weight paraffinic hydrocarbons c50–c60were found, which were not practically detected in the corresponding wax deposits.

关键词： Waxes Asphaltenes Hydrocarbon composition crude oil Wax deposit

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Olefin Polymerization Behavior of Titanium(IV) complexes with Fluorinated and non-fluorinated Aliphatic Phenoxyimine Ligands

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chinese Journal of Polymer Science 2024年第1期42卷 52-62,I0007页

作者： Vladislav A.Tuskaev Kasim F.Magomedov Svetlana ch.Gagieva Dmitry A.Kurmaev yulia V.nelyubina Evgenii K.Golubev Maria D.Evseeva Viktor G.Vasil'ev Galina G.nikiforova Mikhail I.Buzin Vyacheslav S.Bogdanov Boris M.Bulychev Department of chemistry M.V.Lomonosov Moscow State UniversityMoscow 119992Russian Federation A.n.nesmeyanov Institute of Organoelement compounds Russian Academy of SciencesMoscow119991Russian Federation Enikolopov Institute of Synthetic Polymer Materials Russian Academy of SciencesMoscow 117393Russian Federation

A series of Ti(Ⅳ) dichloride and dialkoxide com plexes with phenoxyimine ligands containing fluorinated and nonfluorinated aliphatic imine fragments have been *** molecular structures of complexes 1 and 4 were established by single-crystal X-ray diffraction *** complexes adopt a distorted octahedral coordination structure around the titanium atom and two oxygen atoms are situated in trans position while two nitrogen atoms and two outgoing ligands(cl or iPrO) are situated in cis *** of activators(MMAO-12 and combinations Et_nAIcl_(3-n)+Bu_(2)Mg) and outgoing ligand(cl or iPrO) nature on the catalytic activity and properties of the resulting polymers was *** Ti complexes,despite the nature of the outgoing ligands(cl or iPrO) in the presence of Al/Mg activators,was found to display a high ethylene polymerization activity in the range 1600-3830 kg_(polymer)·mol_(Ti)^(-1)·h^(-1)·atm^(-1) with a viscosity average molecular weight(M_v) value in the range 1.1×10~6-7.1×10~6 Dalton(Da).The resulting UHMWPE can be processed by a solventless method into high-strength and high-modulus oriented *** rheological characteristics of a polymer melt have been *** absence of a cross-over point did not allow to compare the values of the molecular weight distribution of polymers obtained on fluorinated and non-fluorinated pre-catalysts,however,the estimation of the entanglement density is in good agreement with the mechanical characteristics of oriented film *** activation with methylalumoxane,the activity of the com plexes decreased very significantly;however,a polymer with a molecular weight of about 12 million Da was *** the process of ethylene/octene-1 copolymerization,fluorine-containing precatalysts showed a clear advantage over non-fluorinated analogues both in activity and in comonomer content.

关键词： FI catalysts Titanium(IV) (co)polymerization Al/Mg co-catalyst Ultra-high molecular weight polyethylene

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Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality

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Journal of Animal Science and Biotechnology 2023年第1期14卷 114-133页

作者： cláudia Lima Verde Leal karin. canón-Beltrán yulia n.c.jas Meriem Hamdi Aracelli Yaryes María Gemma Millán de la Blanca Paula Beltrán-Brena Rosane Mazzarella Juliano coelho da Silveira Alfonso Gutiérrez-Adán Encina M González Dimitrios Rizos Department of Animal Reproduction National Center Institute for Agriculture and Food Research and Technology(CSIC-INIA)28040 MadridSpain. Departamento de Medicina Veterinaria Faculdade de Zootecnia e Engenharia de AlimentosUniversidade de Sao Paulo(FZEA-USP)PirassunungaBrazil. Facultad de ciencias Agrarias y Ambientales Programa de Medicina VeterinariaFundacion Universitaria Juan de CastellanosTunjaColombia. Laboratorio de Biotecnologia de la Reproduccion Animal Facultad de Ciencias AgropecuariasUniversidad de Cuenca(UC)EC010205 CuencaEcuador. Department of Anatomy and Embryology Veterinary Faculty-Universidad Complutense de Madrid(UCM)MadridSpain.

Background:In vitro production of bovine embryos is a well-established technology,but the in vitro culture(IVc)system still warrants improvements,especially regarding embryo *** study aimed to evaluate the effect of extracellular vesicles(EVs)isolated from oviductal(OF)and uterine fluid(UF)in sequential IVc on the development and quality of bovine *** were cultured in SOF supplemented with either BSA or EVs-depleted fetal calf serum(dFcS)in the presence(BSA-EV and dFcS-EV)or absence of EVs from OF(D1 to D4)and UF(D5 to D8),mimicking in vivo *** from oviducts(early luteal phase)and uterine horns(mid-luteal phase)from slaughtered heifers were isolated by size exclusion *** rate was recorded on days 7-8 and their quality was assessed based on lipid contents,mitochondrial activity and total cell numbers,as well as survival rate after *** mRnA abundance for lipid metabolism-related transcripts and levels of phosphorylated hormonesensitive lipase(pHSL)proteins were also ***,the expression levels of 383 miRnA in OF-and UF-EVs were assessed by ***:Blastocyst yield was lower(Pn BSA treatments compared with dFcS *** rates after vitrification/warming were improved in dFcS-EVs(Pncreased(Pcysts total cell number in dFcS-EV and BSA-EV compared with respective controls(dFcS and BSA),while lipid content was decreased in dFcSEV(Pnd mitochondrial activity did not change(P>0.05).Lipid metabolism transcripts were affected by EVs and showed interaction with type of protein source in medium(PPARGc1B,LDLR,cD36,FASn and PnPLA2,Pn dFcS(Pnty miRnA were differentially expressed between OF-and UF-EVs and only bta-miR-148b was increased in OF-EVs(Pconclusions:Mimicking physiological conditions using EVs from OF and UF in sequential IVc does not affect embryo development but improves blast

关键词： cattle cryopreservation Embryo development Exosomes Lipid metabolism miRnAs Oviduct Uterus

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Genetics of macrovascular complications in type 2 diabetes

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World Journal of Diabetes 2021年第8期12卷 1200-1219页

作者： Ziravard n Tonyan yulia A nasykhova Maria M Danilova Andrey S Glotov Department of Genomic Medicine D.O.Ott Research Institute of ObstetricsGynecology and ReproductologySaint-Petersburg 199034Russia Laboratory of Biobanking and Genomic Medicine of Institute of Translation Biomedicine St.Petersburg State UniversitySaint-Petersburg 199034Russia

Type 2 diabetes mellitus(T2DM)is a metabolic disorder that currently affects more than 400 million worldwide and is projected to cause 552 million cases by the year ***-term vascular complications,such as coronary artery disease,myocardial infarction,stroke,are the leading causes of morbidity and mortality among diabetic *** recent advances in genome-wide technologies have given a powerful impetus to the study of risk markers for multifactorial *** date,the role of genetic and epigenetic factors in modulating susceptibility to T2DM and its vascular complications is being successfully studied that provides the accumulation of genomic *** the future,this will provide an opportunity to reveal the pathogenetic pathways in the development of the disease and allow to predict the macrovascular complications in T2DM *** review is focused on the evidence of the role of genetic variants and epigenetic changes in the development of macrovascular pathology in diabetic patients.

关键词： Type 2 diabetes Epigenetics Genetics Macrovascular complications

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