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Comparative proteomics studies on brown planthopper responding to host rice resistance

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Comparative proteomics studies on brown planthopper respondi...

2016年全国植物生物学大会

作者： kaikai zeng Siqi Wang Juanjuan Zhang Zhifan Yang Hubei Collaborative Innovation Center for Green Transformation of Bio-Resource College of Life SciencesHubei University

The brown planthopper(BPH,Nilaparvata lugens) is one of the most destructive pests of rice. Comparative proteomics analyses of BPH will help to find new measures to control BPH. In this work,comparative proteomics analyses of the fourth instar nymphs of biotype I and II which infested the susceptible rice TN1 and the resistant rice Mudgo,were carried out by isobaric tag-based methodology for relative peptide quantification(iTRAQ) technique. The results revealed that 7385 protein fragments were detected,and 696 fragments were quantified. Total 201 proteins showed obviously different expression levels. These proteins were expressed differently between the two types of BPH,or the same type BPH feeding on the two rice varieties with different resistant levels. 27 proteins were selected to investigate the expression level of the corresponding genes by fluorescent quantitative real-time PCR. The results revealed that most of the genes have the same expression trends with their coding proteins. It is noteworthy that three genes(vitellogenin,fatty acid synthase,protein kinase) were expressed at an extremely different pattern,and probably involved in regulating biotype formation and/or the acute stress reaction. This study will help in intensifying the knowledge of the interaction mechanism of BPH and rice,including: (1) during the acute stress reaction of BPH to host rice resistance,the variation laws of the midgut proteomics,which will indirectly provide some important information about the resistance mechanism of rice;(2) the discrepancy between different biotype BPH midgut proteomics,which will provide some important information for division of biotype and elucidating the molecular mechanism of the formation of a special biotype.

关键词： brown planthopper(Nilaparvata lugens Hemiptera Delphacidae) biotype host rice resistance isobaric tag-based methodology for relative peptide quantification(iTRAQ) comparative proteomics

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浅谈汽车轮胎磨耗试验

汽车实用技术 2021年第16期46卷 110-113页

作者：曾凯凯王伟王广博一汽-大众汽车有限公司天津分公司总装车间天津300000

作为整车的一个组成部件,轮胎的特性对整车性能有着重要影响。目前国内汽车厂还未有规范的轮胎性能试验方法。文章通过对轮胎性能影响参数的分析,并结合德国IFT公司的试验方法,提出了适应中国国情的可行的轮胎磨耗试验规范,从而对轮胎... 详细信息

作为整车的一个组成部件,轮胎的特性对整车性能有着重要影响。目前国内汽车厂还未有规范的轮胎性能试验方法。文章通过对轮胎性能影响参数的分析,并结合德国IFT公司的试验方法,提出了适应中国国情的可行的轮胎磨耗试验规范,从而对轮胎整车性能进行验证。

关键词：轮胎磨耗试验

基于三全育人理念构建《汽车构造Ⅱ》课程思政教学新模式

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时代汽车 2021年第18期 47-48页

作者：王毓曾凯凯石剑飞天津中德应用技术大学汽车与轨道交通学院天津市300350 一汽-大众汽车有限公司天津分公司天津市301500 天津市机电工艺技师学院汽车工程系天津市300350

习近平总书记在全国高校思想政治工作会议上指出,要坚持把立德树人作为中心环节,把思想政治工作贯穿教育教学全过程,实现全员、全程、全方位育人。本文以《汽车构造Ⅱ》课程为载体,探索了如何在专业课程中融入“三全育人”的育人理念,... 详细信息

习近平总书记在全国高校思想政治工作会议上指出,要坚持把立德树人作为中心环节,把思想政治工作贯穿教育教学全过程,实现全员、全程、全方位育人。本文以《汽车构造Ⅱ》课程为载体,探索了如何在专业课程中融入“三全育人”的育人理念,为专业课程思政改革提供新思路。

关键词：三全育人课程思政《汽车构造Ⅱ》

二维功能填料尺寸对BN/EPDM柔性中子防护复合材料性能的影响

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核技术 2024年第9期47卷 102-111页

作者：宗波曾德洋王凯凯唐文偲付嘉佳常树全何佳霖陈芳雷李自平李达国家核安保技术中心北京102401 南京航空航天大学材料科学与技术学院南京211100

柔性中子防护复合材料对于异形构件和人员防护具有重要意义,其性能与功能填料的性质密切相关,但二维功能填料尺寸对材料性能的影响尚不明确。以性能良好的三元乙丙橡胶(Ethylene Propylene Diene Monomer,EPDM)作为柔性基体,以热中子吸... 详细信息

柔性中子防护复合材料对于异形构件和人员防护具有重要意义,其性能与功能填料的性质密切相关,但二维功能填料尺寸对材料性能的影响尚不明确。以性能良好的三元乙丙橡胶(Ethylene Propylene Diene Monomer,EPDM)作为柔性基体,以热中子吸收截面较高的层状氮化硼(Boron Nitride,BN)作为二维功能填料并作表面改性,通过开炼工艺将BN均匀分散于EPDM中,热压硫化后得到BN/EPDM柔性中子防护复合材料,对其微观结构进行表征分析。研究了BN尺寸对复合材料综合性能的影响,结果表明:减小BN尺寸有利于提升复合材料的拉伸强度和热中子屏蔽性能,当材料厚度为2 mm时,其拉伸强度最高可达8.13 MPa,热中子屏蔽率最高可提升约5%。该研究可为聚合物基柔性中子防护复合材料的设计与制备提供基础。

关键词：中子防护复合材料柔性二维功能填料氮化硼

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沉析纤维尺寸和含量对间位芳纶纸性能的影响

合成纤维工业 2024年第2期47卷 30-36页

作者：曹凯凯杨军伍威曹卓杨锡鹏曾勇王进宋欢株洲时代新材料科技股份有限公司湖南株洲412007

使用不同目数的筛网对自制的Z沉析纤维进行筛分,将得到的不同尺寸的沉析纤维与间位芳纶短切纤维按一定质量比配抄成纸,制备间位芳纶纸;通过红外光谱、X射线衍射、扫描电镜及热失重、动态滤水分析对自制的Z沉析纤维的化学结构、热性能、... 详细信息

使用不同目数的筛网对自制的Z沉析纤维进行筛分,将得到的不同尺寸的沉析纤维与间位芳纶短切纤维按一定质量比配抄成纸,制备间位芳纶纸;通过红外光谱、X射线衍射、扫描电镜及热失重、动态滤水分析对自制的Z沉析纤维的化学结构、热性能、动态滤水性能进行表征,并与进口的J沉析纤维对比;研究了沉析纤维的尺寸和含量对间位芳纶纸性能的影响。结果表明:Z沉析纤维与J沉析纤维均为膜状结构,化学结构基本一致,动态滤水性能及热性能相当;经30目或50目的筛网筛分后的沉析纤维可以与短切纤维更好地抱合,间位芳纶纸的抗张强度、断裂伸长率、撕裂度、击穿强度均达到较大值;间位芳纶纸的撕裂度随沉析纤维含量的增加逐渐降低,间位芳纶纸的抗张强度、断裂伸长率、击穿强度随沉析纤维含量的增加呈现先增后降的变化趋势,当沉析纤维质量分数为55%时,间位芳纶纸的抗张强度、断裂伸长率、击穿强度均达到最大值,分别为3.0 kN/m、5.50%、18.7 kV/mm。

关键词：间位芳纶沉析纤维间位芳纶纸力学性能击穿强度

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Gut microbiota-derived short-chain fatty acids ameliorate methamphetamine-induced depression-and anxiety-like behaviors in a Sigmar-1 receptor-dependent manner

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Acta Pharmaceutica Sinica B 2023年第12期13卷 4801-4822页

作者： kaikai Zhang Lijian Chen Jianzheng Yang Jiali Liu Jiahao Li Yi Liu Xiuwen Li Long Chen Clare Hsu Jiahao zeng Xiaoli Xie Qi Wang Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification School of Forensic MedicineSouthern Medical UniversityGuangzhou 510515China Department of Toxicology School of Public HealthSouthern Medical University(Guangdong Provincial Key Laboratory of Tropical Disease Research)Guangzhou 510515China

Methamphetamine(Meth)abuse can cause serious mental disorders,including anxiety and *** gut microbiota is a crucial contributor to maintaining host mental ***,we aim to investigate if microbiota participate in Meth-induced mental disorders,and the potential mechanisms ***,15 mg/kg Meth resulted in anxiety-and depression-like behaviors of mice successfully and suppressed the Sigma-1 receptor(SIGMAR1)/BDNF/TRKB pathway in the ***-while,Meth impaired gut homeostasis by arousing the Toll-like receptor 4(TLR4)-related colonic inflammation,disturbing the gut microbiome and reducing the microbiota-derived short-chain fatty acids(SCFAs).Moreover,fecal microbiota from Meth-administrated mice mediated the colonic inflam-mation and reproduced anxiety-and depression-like behaviors in ***,SCFAs supple-mentation optimized Meth-induced microbial dysbiosis,ameliorated colonic inflammation,and repressed anxiety-and depression-like ***,Sigmarl knockout(Sigmar1^(-/-))repressed the BDNF/TRKB pathway and produced similar behavioral phenotypes with Meth exposure,and elim-inated the anti-anxiety and-depression effects of *** activation of SIGMAR1 with fluvoxamine attenuated Meth-induced anxiety-and depression-like *** findings indicated that gut microbiota-derived SCFAs could optimize gut homeostasis,and ameliorate Meth-induced mental disorders in a SIGMAR1-dependent *** study confirms the crucial role of microbiota in Methrelated mental disorders and provides a potential preemptive therapy.

关键词： Methamphetamine Gut microbiota Short-chain fatty acids Sigma-1 receptor Mental disorders Colonic inflammation Intestinal barrier Gut-brain axis

Efficient Semi‑Transparent Wide‑Bandgap Perovskite Solar Cells Enabled by Pure‑Chloride 2D‑Perovskite Passivation

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Nano-Micro Letters 2023年第8期15卷 35-48页

作者： Liu Yang Yongbin Jin Zheng Fang Jinyan Zhang Ziang Nan Lingfang Zheng Huihu Zhuang Qinghua zeng kaikai Liu Bingru Deng Huiping Feng Yujie Luo Chengbo Tian Changcai Cui Liqiang Xie Xipeng Xu Zhanhua Wei Xiamen Key Laboratory of Optoelectronic Materials and Advanced Manufacturing Institute of Luminescent Materials and Information DisplaysCollege of Materials Science and EngineeringHuaqiao UniversityXiamen 361021People’s Republic of China MOE Engineering Research Center for Brittle Materials Machining Institute of Manufacturing EngineeringCollege of Mechanical Engineering and AutomationHuaqiao UniversityXiamen 361021People’s Republic of China Gold Stone(Fujian)Energy Company Limited Quanzhou 362005People’s Republic of China Collaborative Innovation Center of Chemistry for Energy Materials Department of ChemistryCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005People’s Republic of China

Wide-bandgap(WBG)perovskite solar cells suffer from severe non-radiative recombination and exhibit relatively large opencircuit voltage(V_(OC))deficits,limiting their photovoltaic ***,we address these issues by in-situ forming a well-defined 2D perovskite(PMA)_(2)PbCl_(4)(phenmethylammonium is referred to as PMA)passivation layer on top of the WBG active *** 2D layer with highly pure dimensionality and halide components is realized by intentionally tailoring the side-chain substituent at the aryl ring of the post-treatment ***-principle calculation and single-crystal X-ray diffraction results reveal that weak intermolecular interactions between bulky PMA cations and relatively low cation-halide hydrogen bonding strength are crucial in forming the well-defined 2D ***(PMA)_(2)PbCl_(4)forms improved type-I energy level alignment with the WBG perovskite,reducing the electron recombination at the perovskite/hole-transport-layer *** this strategy in fabricating semi-transparent WBG perovskite solar cells(indium tin oxide as the back electrode),the V_(OC)deficits can be reduced to 0.49 V,comparable with the reported state-of-the-art WBG perovskite solar cells using metal ***,we obtain hysteresis-free 18.60%-efficient WBG perovskite solar cells with a high V_(OC)of 1.23 V.

关键词： Wide-bandgap perovskite solar cells Transparent back electrodes Defect passivation Bulky cations

A novel coral-like garnet for high-performance PEO-based all solid-state batteries

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Science China Materials 2022年第2期65卷 364-372页

作者： Jun Cheng Guangmei Hou Qing Sun Qiong Chen Deping Li Jianwei Li Zhen zeng kaikai Li Qunhui Yuan Jiajun Wang Lijie Ci State Key Laboratory of Advanced Welding and Joining School of Materials Science and EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China Research Center for Carbon Nanomaterials Key Laboratory for Liquid-Solid Structural Evolution&Processing of Materials(Ministry of Education)School of Materials Science and EngineeringShandong UniversityJinan 250061China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China

As one of the most promising next-generation energy storage devices,the lithium-metal battery has been extensively ***,safety issues and undesired lithium dendrite growth hinder its *** application of solid-state electrolytes has attracted increasing attention as they can solve safety issues and show great potential to inhibit the growth of lithium *** oxide(PEO)-based electrolytes are very promising due to their enhanced safety and excellent ***,they suffer from low ionic conductivity at room temperature and cannot effectively inhibit lithium dendrites at high temperatures due to the intrinsic semicrystalline properties and poor mechanical *** this work,a novel coral-like Li_(6.25)Al_(0.25)La_(3)Zr_(2)O_(12)(C-LALZO)is synthesized to serve as an active ceramic filler in *** PEO with LALZO coral(PLC)exhibits increased ionic conductivity and mechanical strength,which leads to uniform deposition/stripping of lithium *** Li symmetric cells with PLC do not cause a short circuit after cycling for 1500 h at 60℃.The assembled LiFePO_(4)/PLC/Li batteries display excellent cycling stability at both 60 and 50℃.This work reveals that the electrochemical properties of the composite electrolyte can be effectively improved by tuning the microstructure of the filler,such as the C-LALZO architecture.

关键词： coral-like garnet composite solid-state electrolyte all-solid-state battery

用于增加三维磁光阱装载率的二维冷原子束系统

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红外与激光工程 2019年第5期48卷 63-68页

作者：曾大吉黄敏章显黄凯凯陆璇辉浙江大学物理学系浙江杭州310027 浙江大学先进技术研究院浙江杭州310027

在原子干涉仪、原子陀螺仪等精密测量的领域中,最基本也是最重要的一步就获得冷原子,而当实验需要连续和高重复性的测量时,对于冷原子的装载就会要求有更快的速率。为了能更快的装载冷原子,就需要一束高通量、低速的冷原子束。在实验上... 详细信息

在原子干涉仪、原子陀螺仪等精密测量的领域中,最基本也是最重要的一步就获得冷原子,而当实验需要连续和高重复性的测量时,对于冷原子的装载就会要求有更快的速率。为了能更快的装载冷原子,就需要一束高通量、低速的冷原子束。在实验上实现了87Rb原子的二维冷却磁光阱(2D-MOT)的冷原子束,其对3D-MOT的装载率为2.8×10~9 atoms/s。该系统基于87Rb原子2D-MOT+push beam方案,选择了红失谐为20 MHz功率为50 m W的两束入射冷却光,在冷却光入射到真空腔之前使用扩束系统将其光斑扩束成短轴为25 mm、长轴为75 mm的椭圆形光斑,在冷却光入射真空腔之后在真空腔的另一端用镀了四分之一波片膜的反射镜来得到对射的激光。

关键词：磁光阱冷原子束 2D-MOT+push