检索结果-南通市图书馆

Expression of matrix metalloprotease and tissue inhibitor of metalloprotease genes in human anterior cruciate ligament fibroblast

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Expression of matrix metalloprotease and tissue inhibitor of...

2008年全国生物流变学与生物力学学术会议

作者： kl paul sung "111"project Laboratory of Biomechanics and Tissue Repair Bioengineering CollegeChongqing UniversityChongqing 400044China Departments of Bioengineering and Orthopaedics University of CaliforniaSan Diego9500 Gilman DriveLa JollaCA 92093-0412USA

Introduction:The incidence of the injury to the knee ligaments has been steadily increased as a result of growing popularity of sport activities. Although all the knee ligaments are somehow involved in knee injuries,regarding treatment the anterior cruciate ligaments is the most *** to its poor healing potential,primary repair of the ligament usually is *** current standard treatment for ACL

关键词：

Signal pathways underlying the MMP-2 production in ACL fibroblast under mechanical injury

在线全文

cnki会议

学校读者我要写书评

暂无评论

Signal pathways underlying the MMP-2 production in ACL fibro...

2008年全国生物流变学与生物力学学术会议

Introduction:Anterior cruciate ligament(ACL) is susceptible to injury and has a poor healing *** current standard treatment for PCL injuries in active adults with functional instability is ligament reconstruction but it does not restore the normal PCL *** is revealed that ligament fibroblasts,especially following ligament injuries release a high amount of matrix metalloproteinases(MMPs) that

关键词：

In vitro study of effects of inflammatory factors and mechanical injury on human synoviocytes on MMP-2 production

在线全文

cnki会议

学校读者我要写书评

暂无评论

In vitro study of effects of inflammatory factors and mechan...

2008年全国生物流变学与生物力学学术会议

Introduction:Human knee joint tissues like anterior cruciate ligament,posterior cruciate ligament,cartilage and meniscus have poor healing *** the MMPs,especially the collagenases,have important roles in the extracellular matrix turnover,by decreasing the production of them during knee injuries,there would be a chance to make the primary repair of PCL and ACL injuries ***

关键词：

An in vitro study to explore the cause of pCL repair failure

在线全文

cnki会议

学校读者我要写书评

暂无评论

An in vitro study to explore the cause of pCL repair failure

2008年全国生物流变学与生物力学学术会议

Introduction:The incidence of the injury to the knee ligaments has been steadily increased as a result of growing popularity of sport activities. Although all the knee ligaments are somehow involved in knee injuries,regarding treatment the cruciate ligaments(anterior and posterior cruciate ligaments or ACL and PCL) are the most *** to their poor healing potential,primary repair of the ligament usually is *** current standard treatment for PCL injuries in active adults with functional instability is ligament reconstruction but it

关键词：

Mechanical Properties of ACL and PCL Ligaments in Aging Rats

在线全文

cnki会议

学校读者我要写书评

暂无评论

Mechanical Properties of ACL and PCL Ligaments in Aging Rats

2007中国生物医学工程联合学术年会

作者：杨力 kl paul sung 重庆大学生物工程学院 University of California San DiegoDepartment of Orthopaedics and Bioengineering 9500 Gilman DriveLa JollaCA 92093-0412 Chongqing University College of Bioengineering

＜正＞INTRODUCTION: Few animals are as well known to the medical community as the Wistar rat. Numerous diseases models are created from this particular species and many of these are genetically engineered to simulate human disorders. However, while there are a number of studies on the knee ligaments in humans and rabbits, there is little literature concerning the Wistar rat. Our group recognizes the potential in the current Wistar models for studies of diseased conditions such as hyperlipidemia, hypertension, and rheumatoid arthritis and their effects on ligament health. The purpose of this study is to develop a baseline model for future studies of these Wistar disease models.

关键词：

Ex Vivo Studies of MMP-2 Profile in Rat ACL Ligament under Mechanical Stretched Injury

在线全文

cnki会议

学校读者我要写书评

暂无评论

Ex Vivo Studies of MMP-2 Profile in Rat ACL Ligament under M...

2007中国生物医学工程联合学术年会

＜正＞INTRODUCTION: Tissue remodeling is a central part to the natural healing process after injury. The timely breakdown and deposition of extracellular matrix （ECM） is important and matrix metalloproteinases （MMPs） have been found to be closely involved in these processes. The expression of most MMPs is transcriptionally regulated by growth factors, hormones, cytokines, and cellular transformation . Unlike most MMPs, MMP-2 is primarily activated on the cell surface and plays an important role in angiogenesis. The healing potential of the anterior cruciate ligament （ACL）, a major ligament contributing to the stability of the knee joint, is extremely poor compared with the medial collateral ligament （MCL） after injury. The first choice in current clinical treatment is ACL reconstruction. Thus, we investigated MMP-2 expression by simulating ACL injury via ex vivo ligament stretching in order to pursue the relationship between MMP-2 level activity, and ACL injury processes.

关键词：

赖氨酰氧化酶家族成员在关节软骨不同分区中的表达

在线全文

cnki会议

学校读者我要写书评

暂无评论

中华创伤杂志 2010年第10期26卷 934-936页

作者：陈浩黄伟 kl paul sung 谢静杨健重庆医科大学附属第一医院骨科 400016 Department of Orthopaedics and Bioengineering University of California San Diego CA *** USA

目的了解赖氨酰氧化酶（lysyl oxidase,LOX）家族成员在关节软骨不同分区中的表达,初步探讨LOX家族成员对关节软骨胶原交联的影响. 方法取成年牛股骨内侧滑车全层软骨块,分为浅表区、过渡区和深区,行RT-PCR测定每区LOX家族成员的表达.... 详细信息

目的了解赖氨酰氧化酶（lysyl oxidase,LOX）家族成员在关节软骨不同分区中的表达,初步探讨LOX家族成员对关节软骨胶原交联的影响. 方法取成年牛股骨内侧滑车全层软骨块,分为浅表区、过渡区和深区,行RT-PCR测定每区LOX家族成员的表达. 结果 LOX、LOXL2、LOXL4由浅表区到深区含量逐区下降,LOXLl、LOXL3无表达. 结论 LOX家族成员在关节软骨不同分区中的表达可能影响胶原在不同分区中的结构及理化特性.

关键词：赖氨酰氧化酶软骨关节髌股关节

维普期刊数据库

钛颗粒对大鼠成骨细胞影响的mRNA差异显示研究

在线全文

学校读者我要写书评

暂无评论

中华外科杂志 2001年第1期39卷 72-75页

作者：马庆军党耕町 H Takei J Kim kl paul sung 北京大学第三临床医院骨科 100083 美国加州大学圣迭戈分校

目的　观察钛金属颗粒对大鼠成骨细胞基因表达的影响 ,寻找与假体周围骨溶解有关的新基因。　方法　在含 10 %牛血清的DMEM培养基中培养Wistar幼鼠成骨细胞 ,达 80 %丰满后加入 0 .1%钛颗粒 ,再培养 2 4h后收获细胞并提取总RNA。用mRNA... 详细信息

目的　观察钛金属颗粒对大鼠成骨细胞基因表达的影响 ,寻找与假体周围骨溶解有关的新基因。　方法　在含 10 %牛血清的DMEM培养基中培养Wistar幼鼠成骨细胞 ,达 80 %丰满后加入 0 .1%钛颗粒 ,再培养 2 4h后收获细胞并提取总RNA。用mRNA差异显示技术比较处理组与对照组之间在mRNA表达上的差别。将有差别的表达带回收、扩增、克隆化 ,用于ReverseNorthern杂交、Northern杂交及测序 ,将测序结果与NCBI数据库比较。　结果　在处理与对照间找到 35条差异表达带 ,经ReverseNorthern杂交得到 5个阳性克隆 ,经测序及NCBI数据库分析 ,其中被命名为G46T的基因与单核细胞趋化蛋白 3达 92 %同源 ,另外 4个是新基因。　结论　钛颗粒可引起鼠成骨细胞基因表达的变化 ;G46T基因可能与假体周围骨溶解有关。

关键词：成骨细胞差异显示钛金属颗粒大鼠关节假体 MRNA

维普期刊数据库

共培养下后交叉韧带成纤维细胞中MMPs的基因表达

在线全文

学校读者我要写书评

暂无评论

第三军医大学学报 2012年第16期34卷 1600-1603页

作者：王春莉梅虎谢静蒋稼欢尹琳陈诚符纯锋 kl paul sung 重庆大学生物工程学院国家"111计划"基地 400044 美国加州大学圣迭亚哥分校骨科与生物工程系美国圣迭亚哥*** 重庆医科大学附属第一医院骨科 400016

目的研究后交叉韧带(posterior cruciate ligament,PCL)成纤维细胞与滑膜细胞共培养下,2种细胞之间的交流对PCL成纤维细胞中基质金属蛋白酶MMP-1、2、3表达及活性的影响。方法分为共培养组和单培养组:①用显微镜观察共培养24 h后细胞的... 详细信息

目的研究后交叉韧带(posterior cruciate ligament,PCL)成纤维细胞与滑膜细胞共培养下,2种细胞之间的交流对PCL成纤维细胞中基质金属蛋白酶MMP-1、2、3表达及活性的影响。方法分为共培养组和单培养组:①用显微镜观察共培养24 h后细胞的活性和迁移率。②分别培养6 h后,提取总RNA,逆转录PCR;实时荧光定量PCR对人后交叉韧带成纤维细胞MMP-1、2、3基因的表达进行半定量和定量分析。③共培养处理1、2、3 d后收集培养上清液,用明胶酶谱法检测MMP-2的活性。结果共培养使PCL细胞和滑膜细胞较单层细胞培养迁移率分别增高了(43.1±0.1)%和(76.2±0.2)%(P<0.01)。与单培养相比,共培养组明显增加了MMP-2的基因表达,但降低了MMP-1、3的基因表达。酶谱结果表明共培养增加了MMP-2活性(P<0.05)。结论共培养能够促进细胞的迁移以及调节细胞中MMP-1、2、3的表达情况。

关键词：后交叉韧带滑膜共培养基质金属蛋白酶

维普期刊数据库