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Protein phosphatase 4 is involved in the late development of <i>Dictyostelium discoideum</i>

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Open Journal of Molecular and Integrative Physiology 2013年第2期3卷 49-53页

作者： Ichiro kamei Kozo Takamoto Naoya Sakuragi Eiji Tanesaka Motonobu Yoshida Department of Agricultural Science Kinki University Nara Japan

A cDNA clone SSJ337 (accession no. AF161253) of 1230 bp, encoding a catalytic subunit of protein phosphatase 4, was selected as one of the clones expressed specifically in prestalk cells from a cDNA library of D. discoideum slugs. Cells transformed with a knockout construct of SSJ337 showed an aberrant and tiny fruiting-body formation with a short stalk. A knockout mutant, SSJ337KO was allowed to develop much slower than a wild-type AX2 after the post-aggregation stage. This suggested that the SSJ337 cDNA clone has played an important role especially in the later development of Dictyostelium discoideum. Results from Northern blotting, analysis showed that transcripts for SSJ337 were accumulated at 16 h to 24 h after starvation began.

关键词： Dictyostelium Protein Phosphatase Fruiting-Body Formation Knockout Mutant

Nighttime enhancement of the amplitude of geomagnetic sudden commencements

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Nighttime enhancement of the amplitude of geomagnetic sudden...

Risk of alcohol use relapse after liver transplantation for alcoholic liver disease

第十一届全国日地空间物理学术讨论会

作者： T.Araki K.Keika T.kamei SOA Key Laboratory for Polar Science Polar Research Institute of China 200129ShangHai Data Analysis Center for Geomagnetism and Space MagnetismGraduate School of ScienceKyoto UniversityKyoto 606-8502Japan Department of Earth and Planetary Science Graduate School of Science Kyoto UniversityKyoto 606-8502Japan Data Analysis Center for Geomagnetism and Space Magnetism Graduate School of Science Kyoto UniversityKyoto 606-8502Japan

＜正＞It is usually considered that geomagetic sudden commencements （SCs） observed in low and middle latitudes take the maximum amplitude around noon. This is reasonable if the enhanced magnetopause and tail current were the main source of the SCs. We present here the following results of the analysis showing that the maximum amplitude actually occur around midnight in low and middle latitudes except the equator.

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World Journal of Gastroenterology 2017年第5期23卷 869-875页

作者： Yasuharu Onishi Hiroyuki Kimura Tomohide Hori Shinichi Kishi Hideya kamei Nobuhiko Kurata Chisato Tsuboi Naoko Yamaguchi Mayu Takahashi Saki Sunada Mitsuaki Hirano Hiroshige Fujishiro Takashi Okada Masatoshi Ishigami Hidemi Goto Norio Ozaki Yasuhiro Ogura Department of Transplantation Surgery Nagoya University Hospital Department of Psychiatry Nagoya University Hospital Transplant Coordination Service Nagoya University Hospital Department of Gastro-enterology and Hepatology Nagoya University Graduate School of Medicine

AIMTo investigate factors, including psychosocial factors, associated with alcoholic use relapse after liver transplantation (LT) for alcoholic liver disease (ALD).METHODSThe clinical records of 102 patients with ALD who were referred to Nagoya University Hospital for LT between May 2003 and March 2015 were retrospectively evaluated. History of alcohol intake was obtained from their clinical records and scored according to the High-Risk Alcoholism Relapse scale, which includes duration of heavy drinking, types and amount of alcohol usually consumed, and previous inpatient treatment history for alcoholism. All patients were assessed for eligibility for LT according to comprehensive criteria, including Child-Pugh score, Model for End-Stage Liver Disease score, and psychosocial *** the 102 patients with ALD referred for LT, seven (6.9%) underwent LT. One (14.3%) of these seven patients returned to heavy drinking, but that patient was able to successfully quit drinking following an immediate intervention, consisting of psychotherapeutic education and supportive psychotherapy, by a psychiatrist. A comparison between the transplantation/registration (T/R) group, consisting of the seven patients who underwent LT and 10 patients listed for deceased donor LT, and 50 patients who did not undergo LT and were not listed for deceased donor LT (non-T/R group), showed statistically significant differences in duration of abstinence period (P < 0.01), duration of heavy drinking (P < 0.05), adherence to medical treatment (P < 0.01), and declaration of abstinence (P < 0.05).CONCLUSIONPatients with ALD referred for LT require comprehensive evaluation, including evaluation of psychosocial criteria, to prevent alcoholic recidivism.

关键词： Liver transplantation Risk assessment Alcoholic liver disease Psychosocial evaluation criteria Liaison psychiatry Alcohol use relapse

Study of the Incorporation Behavior of L-Leu and D-Leu in L-Val Crystallization

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Advances in Chemical Engineering and Science 2016年第3期6卷 262-268页

作者： Jun Sen Norimoto Kokubun Toshimichi kamei Kazushige Ohmori Mitsuhiro Kishino Tatsuki Kashiwagi Masaaki Yokota Norihito Doki Department of Chemistry and Bioengineering Iwate University Morioka Japan Research Institute for Bioscience Products & Fine Chemicals Ajinomoto Co. Inc. Kawasaki Japan Research Institute for Innovation Ajinomoto Co. Inc. Kawasaki Japan

Branched-Chain Amino Acids (BCAAs) are essential amino acids mainly produced through fermentation. With respect to BCAA crystallization, the incorporation behavior of L-form BCAAs as a guest amino acid in the crystallization of L-form BCAAs is well known. However, the incorporation behavior of D-form BCAAs as a guest amino acid in the crystallization of L-form BCAAs is not clear. In this study, we focused on the crystal conformation and incorporation behavior of D-Leucine (D-Leu) and L-leucine (L-Leu) in the crystallization of L-valine (L-Val). Moreover, the difference in the incorporation behavior was investigated on the basis of the distribution ratio of crystals to mother liquor, and the crystal structure and interaction energy were calculated using a molecular dynamics method. The crystal composed of L-Leu in L-Val formed as a solid solution and L-Leu was substituted into the crystal lattice of L-Val. In the case where D-Leu was added as a guest amino acid during L-Val crystallization, D-Leu was not incorporated into the L-Vallattice because the interaction energy between a D-Leu molecule and the L-Val crystal lattice was substantially greater than that between an L-Leu molecule and the L-Val crystal lattice.

关键词： Branched Chain Amino Acid Crystallization L-Valine Leucine Solid Solution

Indocyanine green fluorescence and three-dimensional imaging of right gastroepiploic artery in gastric tube cancer

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World Journal of Gastroenterology 2015年第1期21卷 369-372页

作者： Toru Nakano Tadashi Sakurai Shota Maruyama Yohei Ozawa Takashi kamei Go Miyata Noriaki Ohuchi Division of Advanced Surgical Science and Technology Tohoku University Graduate School of MedicineSendaiMiyagi 980-8574Japan

A 79-year-old male was admitted to our hospital for the treatment of cancer of the gastric *** examination revealed a T1 b Union for International Cancer Control(UICC) tumor at the pyloric region of the gastric *** did not reveal infiltration intothe serosa,peritoneal dissemination,regional lymph node swelling,or distant *** performed a distal gastrectomy preserving the right gastroepiploic artery by referencing the preoperative three-dimensional computed *** also evaluated the blood flow of the right gastroepiploic artery and in the proximal gastric tube by using indocyanine green fluorescence imaging intra-operatively and then followed with a gastrojejunal anastomosis with Roux-en-Y *** definitive diagnosis was moderately differentiated adenocarcinoma of the gastric tube,pT 1bN 0M0,pS tage IA(UICC).His postoperative course was ***-dimensional computed tomographic imaging is effective for assessing the course of blood vessels and the relationship with the surrounding *** evaluation of blood flow of the right gastroepiploic artery and of the gastric tube in the anastomotic portion is very valuable information and could contribute to a safe gastrointestinal reconstruction.

关键词： Gastrectomy Indocyanine green Computed tomography

Optical and Electrochemical Properties of Non-Peripheral Thioaryl-Substituted Subphthalocyanine as Precursors for Dye-Sensitizer to Develop Photovoltaic Cells

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American Journal of Analytical Chemistry 2014年第15期5卷 1037-1045页

作者： Keiichi Sakamoto Satoru Yoshino Makoto Takemoto Kazuhiro Sugaya Hitomi Kubo Tomoe Komoriya Shinnosuke kamei Shigeki Furukawa Department of Sustainable Engineering College of Industrial Technology Nihon University Tokyo Japan Academic Major of Applied Molecular Chemistry Graduate School of Industrial Technology Nihon University Tokyo Japan

Phthalocyanines-related compounds, subphthalocyanines, are the homologues consisting of three isoindole units with boron as the center. The absorption maximum of subphthalocyanines, called the Q band, appears around 560 - 630 nm, which is shifted by approximately 100 nm to shorter wavelengths compared to phthalocyanines. Subphthalocyanines are used as precursors to prepare unsymmetric phthalocyanines for ring enlargement reaction. In this decade, phthalocyanines are used for dye-sensitized solar cells (DSSCs), which require strong absorption of far-red light between 700 and 850 nm because of their highly efficiency. Non-peripheral thioaryl-substituted phthalocyanines have been synthesized. They show near-infrared absorption around 780 - 870 nm and have excellent electron transfer properties. However, their lack of affinity to basal plats inhibits their use as DSSC photosensitizer. Therefore, to synthesize unsymmetrical non-peripheral thioaryl-substituted phthalocyanines possessing good affinity to basal plates, the authors prepared subphthalocyanines having thioaryl substituents as precursors. Spectroscopic properties and electron transfer abilities to synthesize non-peripheral thioaryl-substituted subphthalocyanines were estimated using cyclic voltammetry. The Q band of non-peripheral thioaryl-substituted subphthalocyanines shows around 650 nm shifted to longer wavelength by 86 nm in comparison to subphthalocyanine. The compounds show many reduction potentials. They are acceptable electrons in the subphthalocyanine ring, meaning that the compounds have good electron transfer properties.

关键词： Thioaryl Substituents Phthalocyanines Subphthalocyanine Cyclic Voltammetry Precursor for Dye-Sensitizer

30千兆赫0.25微米栅低噪声 GaAsFET

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微纳电子技术 1981年第3期 58-61页

作者： K.kamei 邓先灿

采用电子束曝光技术刻制栅电极及减小源栅寄生电阻的方法,发展了0.25μm 栅低噪声 GaAsFET。18GHz 下,噪声系数为1.9dB,相应增益为7dB(漏电流为10mA 时),最大有用增益为11dB(漏电流为30mA 时)。30GHz 下,噪声系数为4dB,相应增益为5dB,... 详细信息

采用电子束曝光技术刻制栅电极及减小源栅寄生电阻的方法,发展了0.25μm 栅低噪声 GaAsFET。18GHz 下,噪声系数为1.9dB,相应增益为7dB(漏电流为10mA 时),最大有用增益为11dB(漏电流为30mA 时)。30GHz 下,噪声系数为4dB,相应增益为5dB,最大有用增益为8dB。测得的噪声系数是迄今所报导的最好值。本工作证明,GaAsMESFET 能够在毫米波范围工作。

关键词：噪声系数 GaAsFET 毫米波极高频低噪声 MESFET

Unusual spontaneous improvement in Asian variant of intravascular large B-cell lymphoma

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Open Journal of Internal Medicine 2012年第1期2卷 15-18页

作者： Takashi Ninomiya Toru Nakamura Nobuharu Fujii Akio Hiraki Shigeki Umemura Hiromichi Yamane Atsuko Shirakawa Haruhito kamei Department of Hematology Oncology and Respiratory Medicine Okayama University Hospital Okayama Japan Department of Hematology Oncology and Respiratory Medicine Okayama University Hospital Okayama Japan Department of Medicine Sumitomo-Besshi Hospital Niihama Japan Department of Medicine Sumitomo-Besshi Hospital Niihama Japan Department of Pathology Sumitomo-Besshi Hospital Niihama Japan

The clinical course of the Asian variant of intravascular large B-cell lymphoma (AIVL) is generally very aggressive. We describe a case of AIVL demonstrating an unusual clinical course, with spontaneous improvement. An 81-year-old man with high-grade fever and thrombocytopenia was admitted to our hospital. Although we could not confirm the origin of his symptoms, they disappeared completely without intervention within 2 weeks. Three months later, however, thrombocytopenia reappeared and progressed. Finally, he was readmitted due to a subdural hemorrhage with high fever and he finally died of rapidly progressive multiple organ failure. Autopsy findings revealed the presence of B-cell lymphoma cells in microscopic vessels of many organs as well as hemophagocytosis in the bone marrow. He was diagnosed with AIVL with an unusual indolent clinical course with spontaneous improvement.

关键词： Lymphoma Intravascular Large B-Cell Lymphoma Spontaneous Improvement Hemophagocytosis

An oral fluoropyrimidine agent S-1 induced interstitial lung disease:A case report

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World Journal of Clinical Oncology 2011年第7期2卷 299-302页

作者： Hiromichi Yamane Masahide Kinugawa Shigeki Umemura Yasuhiro Shiote Kenichiro Kudo Toshimitsu Suwaki Haruhito kamei Nagio Takigawa Katsuyuki Kiura Division of Clinical Oncology Sumitomo-Besshi Hospital Cancer Center3-1 Ohji-choNiihamaEhime 792-8543Japan 4th Department of General Medicine Kawasaki Medical School2-1-80 NakasangeOkayama 700-8505Japan Department of Respiratory Medicine Okayama University Hospital2-5-1 Shikata-choOkayamaOkayama 700-8558Japan

A 66-year-old Japanese man with pancreatic cancer received eleven courses of gemcitabine *** tumor responded to gemcitabine until metastatic liver tumors ***,he was treated with S-1,an oral fluoropyrimidine anticancer agent,as salvage ***-two days after initiating S-1,he presented with dyspnea and *** computed tomography showed diffuse interstitial lesions with thickening of the alveolar septa and ground glass *** KL-6 level was elevated to 1,230 U/mL and he did not use any other drugs except ***,the development of interstitial lung disease(ILD)was considered to be due to *** blood oxygen pressure was 49.6 Torr in spite of oxygen administration(5 L/min).Steroid therapy improved his symptoms and the interstitial shadows on chest *** S-1-induced ILD has mostly been reported to be mild,clinicians should be aware that S-1 has the potential to cause fatal ILD.

关键词： Corticosteroid therapy Interstitial lung disease Pancreatic cancer S-1