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褐藻胶的酶法降解及其产物的体外免疫活性

食品科学 2024年第2期45卷 89-96页

作者：章倩戚慧敏卞斌马俊美赖晨欢黄曹兴凌喆勇强南京林业大学江苏省林业资源高效加工利用协同创新中心江苏南京210037

本研究以褐藻胶为底物,探究其在褐藻胶酶法降解过程中的分子质量变化以及酶法降解制备褐藻胶低聚糖的工艺参数,并在此基础上,评价了不同分子质量褐藻胶酶解产物的体外免疫活性。结果表明,褐藻胶经褐藻胶裂解酶降解后分子质量显著下降,... 详细信息

本研究以褐藻胶为底物,探究其在褐藻胶酶法降解过程中的分子质量变化以及酶法降解制备褐藻胶低聚糖的工艺参数,并在此基础上,评价了不同分子质量褐藻胶酶解产物的体外免疫活性。结果表明,褐藻胶经褐藻胶裂解酶降解后分子质量显著下降,通过乙醇分级分离可获得3种不同分子质量的降解产物,其重均分子质量分别为13.4、5.73kDa和3.85kDa。单因素试验优化获得酶法制备褐藻胶低聚糖的最适工艺参数为pH7.0、褐藻胶裂解酶用量15U/g(底物质量计)、酶解时间24h,此时,褐藻胶低聚糖得率为28.05%。利用小鼠巨噬细胞模型对褐藻胶及其3种分子质量降解产物的体外免疫活性进行评价,发现褐藻胶及其酶解产物均具有一定的免疫增强活性,且重均分子质量为5.73kDa的酶解组分免疫增强活性最好,优于褐藻胶低聚糖。同时,通过添加巨噬细胞受体蛋白Toll样受体4(Toll-like receptor 4,TLR4)的阻断剂(TAK-242),验证了褐藻胶酶解产物通过诱导巨噬细胞TLR4的分泌,引起级联反应,增加NO、肿瘤坏死因子-α和白细胞介素6的分泌,从而调节巨噬细胞免疫活性。研究结果可为褐藻胶高值化利用提供理论依据。

关键词：褐藻胶低聚糖免疫活性分子质量褐藻胶酶解产物细胞因子

Determination of 19 polyphenolic compounds in tea by ultra-high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry

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Food Science and Human Wellness 2022年第3期11卷 719-726页

作者： Jian Li junmei ma Yan Zhang Lei Zheng School of Food and Biological Engineering Hefei University of TechnologyHefei 230009China College of Applied Arts and Science Beijing Union UniversityBeijing 100191China Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China Hebei Food Safety Key Laboratory Hebei Food Inspection and Research InstituteShijiazhuang 050227China

A rapid method was presented for the determination of 19 polyphenols in tea by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF MS).Tea samples were extracted by 50%(V/V)ethanol,then separated by Waters Acquity BEH C18 column using a binary solvent system composed of acetonitrile and water(0.1%formic acid)by gradient *** analytes were determined by Q-TOF MS in TOF MS and information dependent acquisition(IDA)-MS/MS *** results showed that mass accuracy error of the 19 polyphenols were lower than 5.0×10^(-6),good linear relationship was got in range of 0.2–500μg/L and correlation coefficient was higher than *** LOD was in the range of 0.002–0.100 mg/kg and the LOQ was in the range of 0.004–0.200 mg/*** of the method was in range of 78.4%–109.2%with spike levels of 0.004–2.000 mg/kg,relative standard deviations were lower than 10%.The method was simple,rapid and *** could be used for the rapid screening and quantitative analysis of 19 polyphenols in tea.

关键词： Ultra-high performance liquid chromatography Quadrupole-time of flight mass spectrometry Tea Polyphenols

Determination of egg and milk allergen in food products by liquid chromatography-tandem mass spectrometry based on signature peptides and isotope-labeled internal standard

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Food Science and Human Wellness 2023年第3期12卷 728-736页

作者： Sufang Fan junmei ma Zhuo Liu Yawei Ning Meicong Cao Qiang Li Yan Zhang Hebei Food Safety Key Laboratory Key Laboratory of Special Food Supervision Technology for State Market RegulationHebei Engineering Research Center for Special Food Safety and HealthHebei Food Inspection and Research InstituteShijiazhuang 050227China College of Bioscience and Bioengineering Hebei University of Science and TechnologyShijiazhuang 050018China

The aim of this work was to develop a liquid chromatography-tandem mass spectrometry method for the determination of milk allergen and egg allergen in food *** peptides GGLEPINFQTAADQAR,VGINYWLAHK,VLVLDTDYK,FFVAPFPEVFGK,and NAVPITPTLNR were confirmed and synthesized as the quantitative peptide of ovalbumin,α-lactalbumin,β-lactoglobulin,α_(S1)-casein andα_(S2)-casein,the relative isotope-labeled internal standards were used in the quantitative *** range was in the range of0.5-5000.0 nmol/L for egg and milk allergen in bread,cake,cookie,rice crust and wheat flour samples with free from egg and milk,the limits of detection of milk allergens and egg allergen were in the range between0.94 mg/100 g and 56.71 mg/100 g,limits of quantification of milk allergens and egg allergen were in the range between 2.36 mg/100 g and 141.78 mg/100 *** recoveries ranged from 76.7%to 122.8%,the relative standard deviations were in the range of 1.60%-15.60%.The developed method has been successfully used for the detection of egg and milk allergen in various food samples.

关键词： Liquid chromatography-tandem mass spectrometry Egg and milk allergen Signature peptides Isotope-labeled internal standards

Rapid analysis of fifteen sulfonamide residues in pork and fish samples by automated on-line solid phase extraction coupled to liquid chromatography–tandem mass spectrometry

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Food Science and Human Wellness 2020年第4期9卷 363-369页

作者： junmei ma Sufang Fan Lei Sun Liangna He Yan Zhang Qiang Li Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China Hebei Food Safety Key Laboratory Hebei Food Inspection and Research InstituteShijiazhuang 050227China

The aim of this work was to develop an automated on-line solid phase extraction(SPE)with liquid chromatography-tandem mass spectrometry method for the detection of fifteen sulfonamides in pork and fish *** were extracted with 0.2%formic acid acetonitrile solution,purified by on-line SPE device with HLB column,then separated by XBridge C18 column,using 0.1%formic acid solution and acetonitrile as the mobile *** spectrometric data was acquired under multiple reaction monitoring(MRM)mode using positive ionization *** standard method was used in the quantification,good linear relationship was got in range of 0.1–100 ng/mL and correlation coefficient was higher than *** limits of detection were in the range of 0.125–2.00g/kg and the limits of quantitation were in the range of 0.250–5.00g/*** of the method were in range of 78.3%–99.3%,relative standard deviation were lower than 10%.The method was simple,sensitivity,and could be used for routine supervision and analysis of fifteen sulfonamides in pork and fish.

关键词： Liquid chromatography–tandem mass spectrometry On-line solid phase extraction Sulfonamides Internal standard quantification

Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry

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Food Science and Human Wellness 2023年第4期12卷 1064-1070页

作者： junmei ma Sufang Fan Liqiong Yang Liangna He Hongwen Zhai Xiaowei Ren Qiang Li Yan Zhang Hebei Food Inspection and Research Institute Hebei Engineering Research Center for Special Food Safety and HealthKey Laboratory of Special Food Supervision Technology for State Market RegulationHebei Food Safety Key LaboratoryShijiazhuang 050200China Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China

A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)were *** samples were extracted with acetonitrile/acetic acid(99:1,V/V),and clean-up by SinChERS-Nano(single-step,cheap,effective,rugged,safe,nano)column,determined by UPLC-Q-TOF/*** accurate mass database and MS/MS database which contains 420 pesticides were established,the automatic retrieval of detection results was carried on according to the accurate mass,retention time,isotope ratio,ion fragment information,and so *** verification was performed on leeks *** results showed that 420 pesticides had good linearity in the range of 0.1-100μg/L,and the correlation coeffificients(R2)was greater than *** limits of detections(LODs)and limits of quantifications(LOQs)of 420 pesticides were in range of 0.05-2.0 and 0.1-5.0μg/L,*** average spike recoveries at 3 levels were 70.1%to 119.7%,and the relative standard deviations(RSD)were lower than 20%(n=6).With this method,a survey of pesticide residues was conducted for 110 samples of 10 different fruits and vegetables,which provided scientific data for ensuring pesticide residue safety of the fruits and vegetables consumed daily by the *** method was simple,sensitive and accurate,and could be used for rapid screening of 420 pesticide residues in fruits and vegetables.

关键词： UPLC-Q-TOF/MS SinChERS-Nano column Pesticide residues Fruits and vegetables

Simultaneous determination of 15 pesticide residues in Chinese cabbage and cucumber by liquid chromatography-tandem mass spectrometry utilizing online turbulent flow chromatography

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Food Science and Human Wellness 2021年第1期10卷 78-86页

作者： Sufang Fan junmei ma Meirong Cao Juan Wang Leilei Zhang Yan Zhang Qiang Li Jia Chen Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China Hebei Food Safety Key Laboratory Hebei Food Inspection and Research Institute050227ShijiazhuangChina College of Food Science and Technology Hebei Agricultural University071001BaodingChina

In this experiment,a liquid chromatography tandem mass spectrometry method was built to determine 15 pesticide residues in Chinese cabbage and cucumber samples based on online turbulent flow chromatography *** modified quick,easy,cheap,effective,rugged,and safe(QuEChERS)extraction,extracts were directly injected to the TLX(TurboFlow Liquid Xcalibur)system and brought to TurboFlow™columns for on-line purification and then transferred to analytical column for further separation and ***™columns types,transfer flow rate,and transfer time were *** of detection and limits of quantification of the method obtained for 15 pesticide residues were ranged between 0.2–1.0μg/kg and 0.5–2.0μg/kg in Chinese cabbage and cucumber *** of pesticide residues were in range of 75.3%–103.7%.matrix effects for 15 pesticides were in range of 5.6%–106.6%.The developed method has been successfully used for the determination of 15 pesticide residues in real samples.

关键词： Pesticide residues Online turbulent flow chromatography Liquid chromatography-tandem mass spectrometry

Determination of icariside,hyperoside and psoralen in food by liquid chromatography-tandem mass spectrometry

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未来食品学报(英文) 2023年第3期3卷 263-272页

作者： Sufang Fan junmei ma Xiaoxian Yuan Xu Wang Yi Wang Yan Zhang Hebei Food Safety Key Laboratory Key Laboratory of Special Food Supervision Technology for State Market RegulationHebei Engineering Research Center for Special Food Safety and HealthHebei Food Inspection and Research InstituteShijiazhuang 050227China Department of Chemical Engineering Key Laboratory for Industrial BiocatalysisMinistry of Education of ChinaTsinghua UniversityBeijing 100083China

A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was built to determine icarside,hyperoside and psoralen in *** samples were extracted with 70%methanol,the solid and semi-solid hotpot seasoning samples were purified by solid phase extraction column,and then determined by HPLC-MS/*** and 0.1%formic acid solution were used as the mobile phase,and the gradient elution was adopted for *** shown in the results,the analytes had good linearity in the range of 0.05−100 ng/mL,and the correlation coeffificients(R^(2))were greater than *** this method,the limits of quantitation(LOQ)of psoralen,icariside and hyperoside in liquid samples were 1.25,25.0 and 12.5μg/L respectively;while the LOQs of psoralen,icariside and hyperoside in solid samples and hotpot seasoning samples were 1.25,25.0 and 12.5μg/kg,*** liquid beverage,solid beverage,health food(in the form of oral liquid,capsule,tablet),integrated alcoholic beverage and solid hotpot seasoning were selected as representative samples and used for method *** average spiked recoveries at 3 levels(LOQ,2 LOQ,10 LOQ)were in the range of 83.7%−115.0%,and the relative standard deviations were in range of 0.5%−9.4%(n=6).The method is rapid,accurate and sensitive,which is suitable for the simultaneous determination of icariside,hyperoside and psoralen in different food matrices.

关键词： Icariside Hyperoside Psoralen Liquid chromatography-tandem mass spectrometry Food samples

Determination of ingredients in livestock and poultry meat based on liquid chromatography-tandem mass spectrometry

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Journal of Future Foods 2022年第1期2卷 53-60页

作者： Liangna He junmei ma Qiang Li Liming Wang Sufang Fan Yan Zhang Hebei Food Safety Key Laboratory Key Laboratory of Special Food Supervision Technology for State Market RegulationHebei Engineering Research Center for Special Food Safety and HealthHebei Food Inspection and Research InstituteShijiazhuang 050227China

A liquid chromatography-tandem mass spectrometry method was established for the determination of ingredients of chicken,duck,pork,beef and mutton.A total of 19 characteristic peptides were screened out from 5 kinds of meat,and a liquid chromatography-tandem mass spectrometry method was established for the determination of characteristic *** accuracy of the method was tested by adding duck,pork and chicken with the mass fractions of 0.5%,1%and 5%to mutton,pork and chicken with the mass fractions of 0.5%,1%and 5%to beef,and duck with the mass fractions of 1%,2%and 10%to *** results show that the method has high accuracy and stability,and could be used to determine the content of 3 kinds of adulterated meat components in mutton and beef samples.

关键词： Liquid chromatography-tandem mass spectrometry Ingredients from livestock and poultry meat Characteristic peptides

Refined acoustic holography via nonlocal metasurfaces

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Science China(Physics,Mechanics & Astronomy) 2024年第7期67卷 113-123页

作者： Shuhuan Xie Hongyu ma junmei Cao Fangshuo Mo Qian Cheng Yong Li Tong Hao Institute of Acoustics School of Physics Science and EngineeringTongji UniversityShanghai 200092China Center for Spatial Information Science and Sustainable Development Applications Tongji UniversityShanghai 200092China College of Surveying and Geo-Informatics Tongji UniversityShanghai 200092China

Holography can provide the desired wavefront phase and/or amplitude for imaging,particle manipulation,bacteria trapping,and cell patterning in optics and ***,previous work on acoustic holography is mostly based on local design optimization,either using active control of the sound source or relying on the structural design to provide the desired *** precise control over the acoustic field remains a significant ***,we realize refined single-plane symmetric binary amplitude,asymmetric intensity gradient amplitude,and bi-objective hologram through the non-local holographic imaging theory that considers the acoustic coupling of structural units in *** taking into account the self-radiation and mutual radiation between many small units on a plate of well-designed thickness,as well as the transmission through the plate's apertures,we can effectively regulate the sound field behind the *** demonstrate the effectiveness of our approach through numerical simulations and experiments,showcasing a circle,a black hole,and a bi-objective with a circle and a square ***,the acoustic black hole hologram precisely reconstructs the intensity gradient distribution at two bright *** non-local holographic imaging theory is valuable for the fine-intensity regulation of the sound field and is expected to be applied in ultrasound diagnosis and treatment,medical imaging,and other fields.

关键词： acoustic holograms non-local interactions fine intensity modulation acoustic metasurfaces