Determination of 19 polyphenolic compounds in tea by ultra-high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry

作     者：Jian Li Junmei Ma Yan Zhang Lei Zheng Jian Li;Junmei Ma;Yan Zhang;Lei Zheng

作者机构：School of Food and Biological EngineeringHefei University of TechnologyHefei 230009China College of Applied Arts and ScienceBeijing Union UniversityBeijing 100191China Hebei Key Laboratory of Forensic MedicineCollege of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China Hebei Food Safety Key LaboratoryHebei Food Inspection and Research InstituteShijiazhuang 050227China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第3期

页      面：719-726页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 070302[理学-分析化学] 090203[农学-茶学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：supported by National Key Research and Development Program of China(2018YFC1603400) 

主　　题：Ultra-high performance liquid chromatography Quadrupole-time of flight mass spectrometry Tea Polyphenols 

摘      要：A rapid method was presented for the determination of 19 polyphenols in tea by ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF MS).Tea samples were extracted by 50%(V/V)ethanol,then separated by Waters Acquity BEH C18 column using a binary solvent system composed of acetonitrile and water(0.1%formic acid)by gradient *** analytes were determined by Q-TOF MS in TOF MS and information dependent acquisition(IDA)-MS/MS *** results showed that mass accuracy error of the 19 polyphenols were lower than 5.0×10^(-6),good linear relationship was got in range of 0.2–500μg/L and correlation coefficient was higher than *** LOD was in the range of 0.002–0.100 mg/kg and the LOQ was in the range of 0.004–0.200 mg/*** of the method was in range of 78.4%–109.2%with spike levels of 0.004–2.000 mg/kg,relative standard deviations were lower than 10%.The method was simple,rapid and *** could be used for the rapid screening and quantitative analysis of 19 polyphenols in tea.