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An array of two periodic leaky-wave antennas with sum and difference beam scanning for application in target detection and tracking

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Frontiers of Information Technology & Electronic Engineering 2023年第4期24卷 567-581页

作者： Mianfeng HUANG juhua liu School of Electronics and Information Technology Sun Yat-sen UniversityGuangzhou 510006China Guangdong Provincial Key Laboratory of Optoelectronic Information Processing Chips and Systems Sun Yat-sen UniversityGuangzhou 510006Chin

An array of two substrate-integrated waveguide(SIW) periodic leaky-wave antennas(LWAs) with sum and difference beam scanning is proposed for application in target detection and tracking. The array is composed of two periodic LWAs with different periods, in which each LWA generates a narrow beam through the n=-1 space harmonic. Due to the two different periods for the two LWAs, two beams with two different directions can be realized, which can be combined into a sum beam when the array is fed in phase or into a difference beam when the array is fed 180?out of phase. The array integrated with 180?hybrid is designed, fabricated, and *** results show that the sum beam can reach a gain up to 15.9 dBi and scan from-33.4?to 20.8?. In the scanning range, the direction of the null in the difference beam is consistent with the direction of the sum beam,with the lowest null depth of-40.8 dB. With the excellent performance, the antenna provides an alternative solution with low complexity and low cost for target detection and tracking.

关键词： Antenna Leaky-wave antenna(LWA) Substrate-integrated waveguide(SIW) Sum and difference beam Target detection and tracking

Molecular identification of the key starch branching enzyme-encoding gene SBE2.3 and its interacting transcription factors in banana fruits

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Horticulture Research 2020年第1期7卷 1354-1368页

作者： Hongxia Miao Peiguang Sun Qing liu juhua liu Caihong Jia Dongfang Zhao Biyu Xu Zhiqiang Jin Key Laboratory of Tropical Crop Biotechnology Ministry of AgricultureInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences571101 HaikouPeople’s Republic of China Key Laboratory of Genetic Improvement of Bananas Haikou Experimental StationChinese Academy of Tropical Agricultural Sciences571101 HaikouHainan ProvincePeople’s Republic of China Commonwealth Scientific and Industrial Research Organization Agriculture and Food CanberraACT 2601Australia College of Horticulture Nanjing Agricultural University210095 NanjingPeople’s Republic of China

Starch branching enzyme(SBE)has rarely been studied in common starchy banana *** the first time,we report here the molecular characterization of seven SBE(MaSBE)and six SBE(MbSBE)genes in the banana A-and B-genomes,respectively,which could be classified into three distinct subfamilies according to genome-wide *** transcriptomic analysis revealed that six MaSBEs and six MbSBEs were expressed in the developing banana fruits of two different genotypes,BaXi Jiao(BX,AAA)and Fen Jiao(FJ,AAB),among which MaSBE2.3 and MbSBE2.3 were highly *** silencing of MaSBE2.3 expression in banana fruit discs led to a significant decrease in its transcription,which coincides with significant reductions in total starch and amylopectin contents compared to those of empty vector *** suggested functional role of MaSBE2.3 in banana fruit development was corroborated by its transient overexpression in banana fruit discs,which led to significant enhancements in total starch and amylopectin contents.A number of transcription factors,including three auxin response factors(ARF2/12/24)and two MYBs(MYB3/308),that interact with the MaSBE2.3 promoter were identified by yeast one-hybrid library *** these ARFs and MYBs,MaARF2/MaMYB308 and MaARF12/MaARF24/MaMYB3 were demonstrated via a luciferase reporter system to upregulate and downregulate the expression of MaSBE2.3,respectively.

关键词： branching starch fruits

MaDREB1F confers cold and drought stress resistance through common regulation of hormone synthesis and protectant metabolite contents in banana

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Horticulture Research 2023年第2期10卷 227-240页

作者： Yi Xu Wei Hu Shun Song Xiaoxue Ye Zehong Ding juhua liu Zhuo Wang Jingyang Li Xiaowan Hou Biyu Xu Zhiqiang Jin Haikou Experimental Station Key Laboratory of Genetic Improvement of BananasSanya Research InstituteState Key Laboratory of Biological Breeding for Tropical CropsInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHainanChina Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources Hainan Institute for Tropical Agricultural ResourcesChinese Academy of Tropical Agricultural SciencesHainanChina Hainan Yazhou Bay Seed Laboratory HainanChina Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions Key Laboratory of Biology and Genetic Resources of Tropical CropsInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHainanChina Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products South Subtropical Crops Research InstituteChinese Academy of Tropical Agricultural SciencesGuangdongChina

Adverse environmental factors severely affect crop *** crop resistance to multiple stressors is an important breeding *** CBFs/DREB1s extensively participate in plant resistance to abiotic stress,the common mechanism underlying CBFs/DREB1s that mediate resistance to multiple stressors remains ***,we show the common mechanism for MaDREB1F conferring cold and drought stress resistance in ***1F encodes a dehydration-responsive element binding protein(DREB)transcription factor with nuclear localization and transcriptional ***1F expression is significantly induced after cold,osmotic,and salt ***1F overexpression increases banana resistance to cold and drought stress by common modulation of the protectant metabolite levels of soluble sugar and proline,activating the antioxidant system,and promoting jasmonate and ethylene *** analysis shows that MaDREB1F activates or alleviates the repression of jasmonate and ethylene biosynthetic genes under cold and drought ***,MaDREB1F directly activates the promoter activities of MaAOC4 and MaACO_(2)0 for jasmonate and ethylene syntheses,respectively,under cold and drought ***1F also targets the MaERF11 promoter to activate MaACO_(2)0 expression for ethylene synthesis under drought ***,our findings offer new insight into the common mechanism underlying CBF/DREB1-mediated cold and drought stress resistance,which has substantial implications for engineering cold-and drought-tolerant crops.

关键词： synthesis sugar finding

Cold stress responsive microRNAs and their targets in Musa balbisiana

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Frontiers of Agricultural Science and Engineering 2016年第4期3卷 335-345页

作者： Jingyi WANG juhua liu Caihong JIA Hongxia MIAO Jianbin ZHANG Zhuo WANG Biyu XU Zhiqiang JIN Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Key Laboratory of Genetic Improvement of Bananas Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences

Cold stress is an environmental factor affecting plant development and production. Recently,micro RNAs(mi RNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although mi RNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of Musa balbisiana grown at normal and low temperature(5°C).A total of 69 known mi RNAs and 32 putative novel mi RNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible mi RNAs were identified through differentially expressed mi RNAs analysis. Among 43 mi RNAs belonging to 26 conserved mi RNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of21 putative novel mi RNAs with altered expression, four were downregulated and 17 upregulated. Furthermore,eight mi RNAs were validated by stem-loop q RT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive mi RNAs were predicted and categorized by *** results provide important information for further characterization and functional analysis of cold-responsive mi RNAs in banana.

关键词： cold stress micro RNA Musa balbisiana

Genome-wide identification and expression analysis of the β-amylase genes strongly associated with fruit development,ripening, and abiotic stress response in two banana cultivars

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Frontiers of Agricultural Science and Engineering 2016年第4期3卷 346-356页

作者： Hongxia MIAO Peiguang SUN Yulu MIAO juhua liu Jianbin ZHANG Caihong JIA Jingyi WANG Zhuo WANG Zhiqiang JIN Biyu XU Key Laboratory of Tropical Crop Biotechnology Ministry of Agriculture Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences Key Laboratory of Genetic Improvement of Bananas Hainan Province Haikou Experimental StationChinese Academy of Tropical Agricultural Sciences Department of Agriculture Hainan University

β-amylase(BAM) is an important enzyme involved in conversion of starch to maltose in multiple biological processes in plants. However, there is currently insufficient information on the BAM gene family in the important fruit crop banana. This study identified 16 BAM genes in the banana genome. Phylogenetic analysis showed that Ma BAMs were classified into four subfamilies. Most Ma BAMs in each subfamily shared similar gene structures. Conserved motif analysis showed that all identified Ma BAM proteins had the typical glyco hydro14 domains. Comprehensive transcriptomic analysis of two banana genotypes revealed the expression patterns of Ma BAMs in different tissues, at various stages of fruit development and ripening, and in responses to abiotic stresses. Most Ma BAMs showed strong transcript accumulation changes during fruit development and late-stage ripening. Some Ma BAMs showed significant changes under cold, salt, and osmotic stresses. This finding indicated that Ma BAMs might be involved in regulating fruit development, ripening, and responses to abiotic stress. Analysis of five hormone-related and seven stressrelevant elements in the promoters of Ma BAMs further revealed that BAMs participated in various biological processes. This systemic analysis provides new insights into the transcriptional characteristics of the BAM genes in banana and may serve as a basis for further functional studies of such genes.

关键词： β-amylase banana genome-wide identification fruit development abiotic stress

Efficient regeneration system applicable to five Musa cultivars

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Frontiers of Agricultural Science and Engineering 2016年第4期3卷 330-334页

作者： juhua liu Peiguang SUN Jing ZHANG Jiashui WANG Jianbin ZHANG Jingyi WANG Caihong JIA Pengzhao GAO Biyu XU Zhiqiang JIN Key Laboratory of Tropical Crop Biotechnology Ministry of Agriculture/Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences

Banana(Musa spp.) is an important staple food, economic crop, and nutritional fruit *** is seriously hampered by the long generation time, polyploidy, and sterility of most *** of an efficient regeneration and transformation system for banana is critical for their genetic improvement. An efficient and reproducible transformation system for banana using direct organogenesis was developed. Media containing benzylaminopurine(BA)combined with one of four other growth regulators was evaluated for the regeneration efficiency of five Musa cultivars and the ability to induce/support development of new banana shoots. The result indicated that the greatest number of shoots per explant for all five Musa cultivars was obtained using MS medium supplemented with8.9 mmol$L–1BA and 9.1 mmol$L–1thidiazuron(TDZ).In 240–270 d, one immature male flower could regenerate between 380 and 456, 310–372, 200–240, 130–156, and100–130 well-developed shoots for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Such a system will facilitate molecular breeding and functional genomics of banana.

关键词： banana(Musa spp) system regeneration

Banana somatic embryogenesis and biotechnological application

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Tropical Plants 2022年第1期1卷 96-108页

作者： Jingyi Wang Shanshan Gan Yunke Zheng Zhiqiang Jin Yunjiang Cheng juhua liu Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of AgricultureInstitute of Tropical Bioscience and Biotechnology/Hainan Key Laboratory for Protection and Utilization of Tropical BioresourcesHainan Institute for Tropical Agricultural Resources/Sanya Research InstituteChinese Academy of Tropical Agricultural Sciences(CATAS)Haikou 571101China Key Laboratory of Horticultural Plant Biology(Ministry of Education) College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070China

As one of the most important economic crops for both staple food and fruit widely cultivated in the tropics and subtropics,banana(Musa spp.)is susceptible to a plethora of abiotic and biotic *** cultivars resistant to abiotic and biotic stressors without adverse effects on yield and fruit quality are the objectives of banana improvement ***,conventional breeding approaches are time-consuming and severely hampered by inherent banana problems(polyploidy and sterility).Therefore,genetic transformation is becoming increasingly popular and can provide rapid *** efforts have been made to develop superior banana cultivars with better resistance to abiotic and biotic stresses and optimum yields using genetic modification *** embryogenesis(SE)through embryogenic cell suspension(ECS)cultures is an ideal recipient system for genetic transformation in *** purpose of this paper is to review the current status of banana somatic embryo research,clarify the process of banana somatic embryo induction and culture,and summarize the main influencing factors in the process of somatic *** the same time,their applications in breeding technologies such as cryopreservation,protoplast culture,genetic transformation and gene editing were also summarized,in order to provide reference for the research and practical application of banana somatic embryogenesis in the future.

关键词： breeding cultivar crops

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广州市小学生龋病现状及影响因素分析

中国学校卫生 2018年第5期39卷 732-734,737页

作者：刘菊华曾素娟广东省广州市中小学卫生健康促进中心 510180 广州医科大学附属口腔医院儿童口腔中心

目的分析二、三年级小学生龋齿患病情况及其影响因素,为制定相关干预措施提供依据。方法分层整群抽取广州市4所小学二、三年级学生1 116名为研究对象,检查其患龋情况,并对学生及其家长进行问卷调查。结果小学生患龋率为72.3%(807/1 116)... 详细信息

目的分析二、三年级小学生龋齿患病情况及其影响因素,为制定相关干预措施提供依据。方法分层整群抽取广州市4所小学二、三年级学生1 116名为研究对象,检查其患龋情况,并对学生及其家长进行问卷调查。结果小学生患龋率为72.3%(807/1 116),其中广州户籍儿童患龋率为52.2%(239/458),非广州户籍儿童为86.3%(568/658),差异有统计学意义(χ~2=157.19,P<0.01);二年级学生患龋率(77.0%)高于三年级(67.7%)(χ~2=12.21,P<0.01)。单因素逐步Logistic回归分析显示,户籍、年级、喝碳酸饮料、食用甜点巧克力、开始刷牙年龄、刷牙次数、刷牙方法、是否使用含氟牙膏、照顾孩子的人、居住环境、医疗来源、父母学历、父母职业、家庭经济收入14个因素均入选方程;多因素Logistic回归分析显示,户籍、喝碳酸饮料、是否使用含氟牙膏及父母学历等均为龋齿发生的独立影响因素(P值均<0.01)。结论二、三年级小学生是龋病发生的高危群体,龋齿发生与社会生活环境、饮食习惯、口腔卫生习惯等因素关系密切。

关键词：回归分析学生龋齿患病率儿童口腔保健

MuMADS1 and MaOFP1 regulate fruit quality in a tomato ovate mutant

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MuMADS1 and MaOFP1 regulate fruit quality in a tomato ovate ...