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Molecular identification of the key starch branching enzyme-encoding gene SBE2.3 and its interacting transcription factors in banana fruits

作     者:Hongxia Miao Peiguang Sun Qing Liu Juhua Liu Caihong Jia Dongfang Zhao Biyu Xu Zhiqiang Jin Hongxia Miao;Peiguang Sun;Qing Liu;Juhua Liu;Caihong Jia;Dongfang Zhao;Biyu Xu;Zhiqiang Jin

作者机构:Key Laboratory of Tropical Crop BiotechnologyMinistry of AgricultureInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences571101 HaikouPeople’s Republic of China Key Laboratory of Genetic Improvement of BananasHaikou Experimental StationChinese Academy of Tropical Agricultural Sciences571101 HaikouHainan ProvincePeople’s Republic of China Commonwealth Scientific and Industrial Research Organization Agriculture and FoodCanberraACT 2601Australia College of HorticultureNanjing Agricultural University210095 NanjingPeople’s Republic of China 

出 版 物:《Horticulture Research》 (园艺研究(英文))

年 卷 期:2020年第7卷第1期

页      面:1354-1368页

核心收录:

学科分类:09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基  金:sponsored by the National Key R&D Program of China(No.2018YFD1000200 and 2019YFD1000200) the Modern Agro-industry Technology Research System of China(No.CARS-31) the Central Publicinterest Scientific Institution Basal Research Fund(Nos.1630052020002,1630052017010,and 1630052016006) the National Natural Science Foundation of China(NSFC,No.31401843) 

主  题:branching starch fruits 

摘      要:Starch branching enzyme(SBE)has rarely been studied in common starchy banana *** the first time,we report here the molecular characterization of seven SBE(MaSBE)and six SBE(MbSBE)genes in the banana A-and B-genomes,respectively,which could be classified into three distinct subfamilies according to genome-wide *** transcriptomic analysis revealed that six MaSBEs and six MbSBEs were expressed in the developing banana fruits of two different genotypes,BaXi Jiao(BX,AAA)and Fen Jiao(FJ,AAB),among which MaSBE2.3 and MbSBE2.3 were highly *** silencing of MaSBE2.3 expression in banana fruit discs led to a significant decrease in its transcription,which coincides with significant reductions in total starch and amylopectin contents compared to those of empty vector *** suggested functional role of MaSBE2.3 in banana fruit development was corroborated by its transient overexpression in banana fruit discs,which led to significant enhancements in total starch and amylopectin contents.A number of transcription factors,including three auxin response factors(ARF2/12/24)and two MYBs(MYB3/308),that interact with the MaSBE2.3 promoter were identified by yeast one-hybrid library *** these ARFs and MYBs,MaARF2/MaMYB308 and MaARF12/MaARF24/MaMYB3 were demonstrated via a luciferase reporter system to upregulate and downregulate the expression of MaSBE2.3,respectively.

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