Efficient regeneration system applicable to five Musa cultivars
Efficient regeneration system applicable to five Musa cultivars作者机构：Key Laboratory of Tropical Crop Biotechnology Ministry of Agriculture/Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences
出 版 物：《Frontiers of Agricultural Science and Engineering》 (农业科学与工程前沿（英文版）)
年 卷 期：2016年第3卷第4期
页 面：330-334页
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0836[工学-生物工程] 0902[农学-园艺学] 090201[农学-果树学]
基 金：supported by the earmarked fund for Modern Agro-Industry Technology Research System (CARS-32) the National Nonprofit Institute Research Grant of the Institute of Tropical Bioscience and Biotechnology CATAS-ITBB (ITBB2015ZY06)
主 题：banana(Musa spp) system regeneration
摘 要：Banana(Musa spp.) is an important staple food, economic crop, and nutritional fruit *** is seriously hampered by the long generation time, polyploidy, and sterility of most *** of an efficient regeneration and transformation system for banana is critical for their genetic improvement. An efficient and reproducible transformation system for banana using direct organogenesis was developed. Media containing benzylaminopurine(BA)combined with one of four other growth regulators was evaluated for the regeneration efficiency of five Musa cultivars and the ability to induce/support development of new banana shoots. The result indicated that the greatest number of shoots per explant for all five Musa cultivars was obtained using MS medium supplemented with8.9 mmol$L–1BA and 9.1 mmol$L–1thidiazuron(TDZ).In 240–270 d, one immature male flower could regenerate between 380 and 456, 310–372, 200–240, 130–156, and100–130 well-developed shoots for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Such a system will facilitate molecular breeding and functional genomics of banana.
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