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Biomineralization Precursor carrier System Based on carboxyl- Functionalized Large Pore Mesoporous Silica Nanoparticles

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current Medical Science 2020年第1期40卷 155-167页

作者： Sheng WEI Hua WU Xiao-juan LUO Department of Orthopedics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Stomatology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Bone and teeth are derived from intrafibrillarly mineralized collagen fibrils as the second level of *** to polymer-induced liquid-precursor process,using amorphous calcium phosphate precursor(AcP)is able to achieve intrafibrillar mineralization in the case of bone biomineral in ***,AcP precursors might be blended with any osteoconductive scaffold as a promising bone formation supplement for in-situ remineralization of collagens in *** this study,mesoporous silica nanoparticles with carboxyl-functionalized groups and ultra large-pores have been synthesized and used for the delivery of liquid like biomimetic precursors(AcP).The precursor delivery capacity of the nanoparticles was verified by the precursor release profile and successful mineralization of 2D and 3D collagen *** nanoparticles could be completely degraded in 60 days and exhibited good biocompatibility as *** successful translational strategy for biomineralization precursors showed that biomineralization precursor laden ultra large pore mesoporous silica possessed the potential as a versatile supplement in demineralized bone formation through the induction of intrafibrillar collagen mineralization.

关键词： biomineralization mesoporous silica amorphous calcium phosphate biocompatibility biodegradability carboxyl functionalization

Daily expression of sodium-dependent glucose cotransporter-1protein in jejunum during rat ontogeny

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Animal Nutrition 2019年第3期5卷 290-296页

作者： juan I.Baston Fabricio D.cid Enrique caviedes-Vidal juan G.chediack Laboratorio de Fisiopatología Endometrial Instituto de Biología y Medicina Experimental(IByME)CONICETCiudad Autoonoma de Buenos AiresC1428ADNArgentina Laboratorio de Biología Integrativa“Prof.E.caviedes codelia” Facultad de QuímicaBioquímica y FarmaciaUniversidad Nacional de San LuisSan LuisD5700HHWArgentina Instituto Multidisciplinario de Investigaciones en Biología-San Luis(IMIBIO-SL CCT-SL)CONICETSan Luis D5700HHWArgentina

It is widely known that intestinal capacities such as the enzymatic hydrolysis of carbohydrates,lipids and proteins,and the subsequent absorption of the hydrolyzed *** evolutionary matched to dietary loads and feeding *** this study,we demonstrate that the protein expression of apically located sodium-dependent glucose cotransporter-1(SGLT-1)throughout rat ontogeny is daily adjusted to afford glucose uptake when the load of this metabolically essential monosaccharide in the intestinal lumen is *** jejunal expression of SGLT-1 protein in 14 one-day-old suckling pups was found to increase at dark and early light phase(Pccess to mother *** weaning21-d-old and juvenile 28-d-old rats,the cotransporter expression was high throughout the entire day(Pcircadian rhythm for SGLT-1 protein(Pcreased at late light and dark phase when the highest intestinal glucose load was *** our knowledge,these results are the first reporting the daily profile of SGLT-1 expression during rat early developmental stage and may contribute to understand the biological significance of a well-established molecular capacity to deal with the crucial increase of glucose load in the diet during the weaning process.

关键词： Intestinal SGLT-1 Protein expression Daily rhythms Ontogeny Rat

Evolution of Artificial Base Pairs with Hydrogen Bond complementarity

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chemical Research in chinese Universities 2020年第2期36卷 151-156页

作者： TIAN Jimniao cHEN Sikai WANG Xiang LI juan Institute of Molecular Medicine State Key laboratory of Oncogenes and Related GenesRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127P.R.China Key Laboratory for Analytical Science of Food Safety and Biology Ministry of EducationFujian Provincial Key Laboratory of Analysis and Detection Technology for Food SafetyState Key Laboratory of Photocatalysis on Energy and EnvironmentCollege of ChemistryFuzhou UniversityFuzhou 350108.P.R.China

Artificial base pairs,from the perspective of synthetic biology,are designed to contain the features of modularity,orthogonality,and *** the development of artificial base pairs has beat endowed with responsibility to understand the biological process,improve the recognition capacity and stability of aptamers,and develop the nucleoside drugs,diagnosis,and drag *** this review,we first gave a concise introduction of artificial base pairs based on their interaction modes including alternative hydrogen bonding,hydrophobic interaction,and metal *** we displayed the detailed information of artificial base pairs with hydrogen bonding interaction,and analyzed how the changes of their structures affect their ***,we highlighted the applications of functional artificial base pairs in aptamer discovery,diagnosis,and drug ***,an insight into the remaining challenges and future perspective of the artificial bases was provided.

关键词： Artificial base pair Nucleic add Hydrogen bonding base pair Hydrophobic base pair Metal-base pair Aptamcr

nNOS and Neurological, Neuropsychiatric Disorders: A 20-Year Story

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Neuroscience Bulletin 2023年第9期39卷 1439-1453页

作者： Li-juan Zhu Fei Li Dong-Ya Zhu Key Laboratory of Developmental Genes and Human Diseases Ministry of EducationDepartment of Histology and EmbryologySchool of MedicineSoutheast UniversityNanjing210009China Department of Pharmacology School of PharmacyNanjing Medical UniversityNanjing211166China Department of Medicinal chemistry School of PharmacyNanjing Medical UniversityNanjing211166China

In the central nervous system,nitric oxide(NO),a free gas with multitudinous bioactivities,is mainly produced from the oxidation of L-arginine by neuronal nitric oxide synthase(nNOS).In the past 20 years,the studies in our group and other laboratories have suggested a significant involvement of nNOS in a variety of neurological and neuropsychiatric *** particular,the interactions between the PDZ domain of nNOS and its adaptor proteins,including post-synaptic density 95,the carboxy-terminal PDZ ligand of nNOS,and the serotonin transporter,significantly influence the subcellular localization and functions of nNOS in the *** nNOS-mediated protein-protein interactions provide new attractive targets and guide the discovery of therapeutic drugs for neurological and neuropsychiatric ***,we summarize the work on the roles of nNOS and its association with multiple adaptor proteins on neurological and neuropsychiatric disorders.

关键词： nNOS nNOS-PSD95 nNOS-cAPON nNOS-SERT Neurological and neuropsychiatric disorder

H9N2 AIV灭活疫苗免疫SPF鸡攻毒后肺组织免疫相关基因表达变化研究

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南京农业大学学报 2024年第1期47卷 18-27页

作者：李丽蒋作仪李茵婧常丽凤辛震东平继辉苏娟南京农业大学动物医学院江苏南京210095

[目的]本试验旨在探究H9N2亚型禽流感病毒攻击对H9灭活疫苗免疫SPF鸡呼吸系统的影响。[方法]选用36只3周龄SPF鸡,随机分为对照组(con)、攻毒组(Flu)、免疫后攻毒组(Vac+Flu)。以每只0.4 mL剂量免疫接种H9灭活疫苗,3周后以10~6 EID_(50)... 详细信息

[目的]本试验旨在探究H9N2亚型禽流感病毒攻击对H9灭活疫苗免疫SPF鸡呼吸系统的影响。[方法]选用36只3周龄SPF鸡,随机分为对照组(con)、攻毒组(Flu)、免疫后攻毒组(Vac+Flu)。以每只0.4 mL剂量免疫接种H9灭活疫苗,3周后以10~6 EID_(50)的病毒量进行攻毒,采集拭子、血清、气管、肺组织等样品,通过血凝抑制(HI)试验、RT-PcR、荧光定量PcR(qPcR)等方法检测血清中HI抗体滴度、肺脏流感病毒M基因拷贝数以及免疫相关基因cD4、cD8、GATA3、T-bet、IL-4、IL-13、TNF-α、IFN-γ、Perforin、Granzyme、FasL、Fas等的表达量;并利用HE染色、免疫组织化学的方法观察气管、肺脏的病理变化及病毒特异性抗原NP蛋白的分布特征。[结果]HI试验结果显示,SPF鸡疫苗免疫后可产生较高的H9N2 AIV抗体效价。免疫保护试验和RT-PcR结果显示,SPF鸡疫苗免疫后攻毒仍能检测到机体排毒和流感病毒M基因在肺脏组织中的表达。HE染色和免疫组化结果显示,接种H9N2 AIV灭活疫苗后能够明显减轻SPF鸡气管和肺脏的病理损伤,降低气管、肺脏中的病毒载量。荧光定量PcR结果显示,接种H9N2 AIV灭活疫苗后能够提高SPF鸡肺脏中Th1、Th2细胞因子分泌水平,促进穿孔素/颗粒酶途径相关基因表达进而增强肺脏中细胞毒性反应。[结论]H9N2疫苗免疫鸡感染H9病毒后虽然仍存在排毒现象,但其抗病毒免疫系统活跃、其主要器官病毒载量降低,建议按照流感疫苗免疫程序进行免疫接种,提高对流感的防控水平。

关键词： SPF鸡 H9N2亚型禽流感病毒灭活疫苗肺免疫相关基因

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Acquisition of functional neurons by direct conversion:Switching the developmental clock directly

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Journal of Genetics and Genomics 2019年第10期46卷 459-465页

作者： Shuangquan chen juan Zhang Dongming Zhang Jianwei Jiao Institute of Zoology Chinese Academy of SciencesBeijing100101China School of Pharmaceutical Sciences Tsinghua UniversityBeijing100084China University of chinese Academy of Sciences Beijing100049China co-Innovation center of Neuroregeneration Nantong UniversityNantong226001China Institute for Stem cell and Regeneration Chinese Academy of SciencesBeijing100101China

Identifying approaches for treating neurodegeneration is a thorny task but is important for a growing number of *** have focused on discovering the underlying molecular mechanisms of reprogramming and optimizing the technologies for acquiring *** conversion is one of the most important processes for treating neurological *** neurons derived from direct conversion,which bypass the pluripotency stage,are more effective,more quickly obtained,and are safer than those produced via induced pluripotent stem cells(iPScs).Based on iPSc strategies,scientists have derived methods to obtain functional neurons by direct conversion,such as neuron-related transcriptional factors,small ***,and epigenetic *** this review,we discuss the present strategies for direct conversion of somatic cells into functional neurons and the potentials of direct conversion for producing functional neurons and treating neurodeeeneration.

关键词： Direct conversion Fibroblasts Induced neurons Neurodegeneration

用于尿素氧化的介孔Ni-NiO@Nc电极构造

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精细化工中间体 2024年第1期54卷 59-65页

作者：盛飞龙张雨娟胡拖平中北大学化学化工学院山西太原030051 太原工学院山西太原030008

电催化尿素氧化反应(UOR)可作为直接尿素燃料电池(DUFc)的阳极反应。采用水热联合热解的方法,在介孔含氮碳基体(Nc)上构建了锚定Ni和NiO晶体异质结的UOR电催化剂。在1.67 V时,优化工艺合成的Ni-NiO@Nc-500复合材料的碱性介质UOR阳极峰... 详细信息

电催化尿素氧化反应(UOR)可作为直接尿素燃料电池(DUFc)的阳极反应。采用水热联合热解的方法,在介孔含氮碳基体(Nc)上构建了锚定Ni和NiO晶体异质结的UOR电催化剂。在1.67 V时,优化工艺合成的Ni-NiO@Nc-500复合材料的碱性介质UOR阳极峰值电流密度为237 mA/cm2,在10 mA/cm2的UOR的电势为1.38 V(vs RHE)。12 h的cA循环电流密度>80%。Ni-NiO@Nc-500良好的电催化活性关键在于Ni和NiO之间形成了异质结,界面处存在协同作用,Ni/NiO异质结不仅提供了更多的UOR活性位点,而且有利于尿素吸附和气体产物解吸。此外,N掺杂调节了复合材料的电子密度,加快了UOR过程中的电子传递,其介孔结构有利于电解质的渗透和电子转移,其Nc提高了催化剂的稳定性。Ni-NiO@Nc-500材料将有望成为一种高效、低成本的UOR电催化剂。

关键词：电催化活性水热联合热解 Ni/NiO异质结氮掺杂碳尿素氧化反应

基于智能合约和cPSO_DNN的流程工艺参数可信自决策模型

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控制与决策 2024年第6期39卷 1869-1878页

作者：刘孝保袁智慧张雨东孙海彬阴艳超姚廷强顾文娟昆明理工大学机电工程学院昆明650500

针对流程制造过程中工艺关系复杂、优化效率难保证和数据安全问题,提出一种基于智能合约和改进混沌粒子群算法的工艺参数可信自决策模型PPO-TS.首先,基于区块链技术设计适合流程制造工艺特性的数据集成与存储机制,通过数据上链技术实现... 详细信息

针对流程制造过程中工艺关系复杂、优化效率难保证和数据安全问题,提出一种基于智能合约和改进混沌粒子群算法的工艺参数可信自决策模型PPO-TS.首先,基于区块链技术设计适合流程制造工艺特性的数据集成与存储机制,通过数据上链技术实现数据的可信存储;然后,设计工艺参数自决策智能合约机制,利用智能合约搭建基于区块链广播式通信协议的工艺参数优化网络,启动网络并编译质量指标访问、优化自决策和决策自执行智能合约,通过自动触发工艺参数优化事务完成自决策和自执行过程;在此基础上构建基于改进混沌粒子群算法cPSO和深度神经网络DNN的优化算法cPSO_DNN,实现流程制造工艺参数优化;最后,以现场采集的某流程生产线数据为例,验证了PPO-TS模型的实用性和有效性,为流程制造工艺参数优化提供了一种新思路.

关键词：流程制造区块链智能合约数据可信存储工艺参数自决策工艺参数优化

Existence and Uniqueness of Fixed Point in Partially Ordered Sets and Applications to Ordinary Differential Equations

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Acta Mathematica Sinica,English Series 2007年第12期23卷 2205-2212页

作者： juan J.NIETO RosanaRODRGUEZ-LPEZ Departamento de Análisis Matemático Facultad de MatemáticasUniversidad de Santiago de Compostela

We prove some fixed point theorems in partially ordered sets, providing an extension of the Banach contractive mapping theorem. Having studied previously the nondecreasing case, we consider in this paper nonincreasing mappings as well as non monotone mappings. We also present some applications to first-order ordinary differential equations with periodic boundary conditions, proving the existence of a unique solution admitting the existence of a lower solution.

关键词： fixed point partially ordered set first-order differential equation lower and upper solutions