检索结果-南通市图书馆

Detection of.hyper-conserved regions in hepatitis B virus X gene potentially usef.l f.r gene therapy

World Journal of.Gastroenterology 2018年第19期24卷 2095-2107页

作者： Carolina González David Tabernero Maria f.ancesca Cortese Josep Gregori Rosario Casillas Mar Riveiro-Barciela Cristina godoy Sara Sopena Ariadna Rando Marcal Yll Rosa Lopez-Martinez Josep Quer Raf.el Esteban Maria Buti f.ancisco Rodríguez-f.ías Liver Pathology Unit Departments of Biochemistry and MicrobiologyHospital Universitari Vall d’HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Liver Unit Liver Disease Laboratory-Viral HepatitisVall d’Hebron Institut Recerca-Hospital Universitari Vall d’HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Centro de Investigación Biomédica en Red de Enf.rmedades Hepáticas y Digestivas(CIBERehd) Instituto de Salud Carlos IIIMadrid 28029Spain Roche Diagnostics SL Sant Cugat del Vallès 08174Spain Liver Unit Department of Internal MedicineHospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain

AIM To detect hyper-conserved regions in the hepatitis B virus(HBV) X gene(HBX) 5' region that could be candidates f.r gene *** The study included 27 chronic hepatitis B treatmentnaive patients in various clinical stages(f.om chronic inf.ction to cirrhosis and hepatocellular carcinoma, both HBeA g-negative and HBeA g-positive), and inf.cted with HBV genotypes A-f.and H. In a serum sample f.om each patient with viremia > 3.5 log IU/m L, the HBX 5' end region [nucleotide(nt) 1255-1611] was PCRamplif.ed and submitted to next-generation sequencing(NGS). We assessed genotype variants by phylogenetic analysis, and evaluated conservation of.this region by calculating the inf.rmation content of.each nucleotide position in a multiple alignment of.all unique sequences(haplotypes) obtained by NGS. Conservation at the HBx protein amino acid(aa) level was also *** NGS yielded 1333069 sequences f.om the 27 samples, with a median of.4578 sequences/sample(2487-9279, IQR 2817). In 14/27 patients(51.8%), phylogenetic analysis of.viral nucleotide haplotypes showed a complex mixture of.genotypic variants. Analysis of.the inf.rmation content in the haplotype multiple alignments detected 2 hyper-conserved nucleotide regions, one in the HBX upstream non-coding region(nt 1255-1286) and the other in the 5' end coding region(nt 1519-1603). This last region coded f.r a conserved amino acid region(aa 63-76) that partially overlaps a Kunitz-like *** Two hyper-conserved regions detected in the HBX 5' end may be of.value f.r targeted gene therapy, regardless of.the patients' clinical stage or HBV genotype.

关键词： Hepatitis B virus Hepatitis B X gene Hepatitis B X protein Gene therapy Next-generation sequencing HBV conserved regions Small interf.rence RNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Prevalence and Outcomes of.Inf.uenza Virus Inf.ction in Heart f.ilure Patients in Brazil: Inf.uenza Inf.ction in Heart f.ilure

引用

Advances in Inf.ctious Diseases 2015年第3期5卷 95-100页

作者： Henrique godoy Juliana Soares Paula Zanellatto Neves N.C.J. Bellei Dirceu R. Almeida Division of.Cardiology Department of Medicine Federal University of Sao Paulo Sao Paulo Brazil Hospital Epidemiology Committee Federal University of Sao Paulo Sao Paulo Brazil Clinical Virology Laboratory Division of Infectious Diseases Department of Medicine Federal University of Sao Paulo Sao Paulo Brazil

Background: Respiratory tract inf.ctions were associated with acute exacerbations of.heart f.ilure (Hf.. However, the role of.the inf.uenza virus, a major agent of.such inf.ctions, in this population remained unclear. Method: During the inf.uenza virus seasons of.2013 and 2014 we prospectively assessed inf.uenza respiratory illnesses in a cohort of.adults primarily hospitalized f.r management of.acute decompensated Hf.and a cohort of.Hf.outpatients. Qualitative RT-PCR f.r?inf.uenza?A (A/H1, A/H12009pdm, A/H3) and B virus testing was perf.rmed on nasopharyngeal swab samples. Result: A total of.121 patients were included, 58.3% males (n?= 70), mean age 57.7 years old (±14.0), mean lef. ejection f.action 35.3 (±9.8). Of.these, 50.4% were inpatients (n?= 61). The prevalence of.symptoms of.respiratory inf.ctions was 28.0% (n?= 34) and 4.9% (n?= 6) of.all samples were positive f.r inf.uenza virus. Only inf.uenza A was detected and all cases were among inpatients. Inf.uenza-positive patients had a greater need f.r antimicrobials (83.3%,?n?= 5;16.3%,?n= 9;?p?= 0.001) and f.r mechanical ventilation (50.0%,?n?= 3;3.6%,?n?= 2;?p?f.uenza-negative patients. The prevalence of.inf.uenza virus was not related to mortality (OR 4.58;?p?= 0.16). Conclusion:?Although not common, the inf.uenza virus inf.ction resulted in worst outcomes, with a greater need f.r antimicrobials and mechanical ventilation. Immunization and antiviral treatment in high risk patients may positively impact their outcomes.

关键词： Inf.uenza Virus Heart f.ilure Epidemiology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

水泥丛林中的蝴蝶

引用

照明设计 2009年第1期 94-98页

作者： David Muller Plinio godoy（摄影）

提到圣保罗，就会联想起狂欢节、桑巴舞和各种游街盛典，而有关这个城市现代建筑和设计的记忆却很淡。建筑师若昂·瓦伦特（Joao Valente）设计的奥利维亚大桥（*** f.iasde Oliveira Bridge）向人们展示出巴西大城市应有的风采。大桥... 详细信息

提到圣保罗，就会联想起狂欢节、桑巴舞和各种游街盛典，而有关这个城市现代建筑和设计的记忆却很淡。建筑师若昂·瓦伦特（Joao Valente）设计的奥利维亚大桥（*** f.iasde Oliveira Bridge）向人们展示出巴西大城市应有的风采。大桥横跨皮涅鲁（Pinheiros）河，像一只奇异的蝴蝶振翅飞翔在亚马逊州上空。

关键词：蝴蝶丛林水泥现代建筑大城市建筑师 vio 设计

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

f.aud investigation in commercial cof.ee by chromatography

引用

f.od Quality and Saf.ty 2018年第3期2卷 121-133页

作者： Víctor de Carvalho Martins Ronoel Luiz de Oliveira godoy Ana Cristina Miranda Senna Gouvêa Manuela Cristina Pessanha de Araujo Santiago Renata Galhardo Borguini Elaine Cristina de Oliveira Braga Sidney Pacheco Luzimar da Silva de Mattos do Nascimento Departamento de Tecnologia de Alimentos Instituto de TecnologiaUniversidade Federal Rural do Rio de JaneiroCEP 23890-000Seropédica-RJBrazil Laboratório de Cromatograf.a Líquida Embrapa Agroindústria de AlimentosCEP 23020-470Rio de Janeiro-RJBrazil Centro de Tecnologia Instituto de QuímicaUniversidade Federal do Rio de JaneiroCEP 22290-180Rio de Janeiro-RJBrazil

Cof.ee is currently the second largest commodity on the world market today,and there is great concern about the quality of.the beans exported f.om producer countries to Europe and *** such as using blends of.dif.erent species and adding low-cost raw materials,such as chicory,corn,and soybean,impair the sensory and f.nctional characteristics of.the drink made f.om roasted and ground cof.ee *** is a need to adopt more ef.icient analytical methods than the microscopy technique currently *** f.rst chromatographic method used to determine f.aud was reported in *** method used paper chromatography to dif.erentiate between cof.ee and chicory based on the f.ee reducing *** of.the 1980s,dif.erent methods involving high-perf.rmance liquid chromatography and gas chromatography were developed in order to demonstrate geographic authenticity,distinction between species,occurrence of.adulteration,and the presence of.def.ctive beans by determining the monosaccharides,oligosaccharides,tocopherols,f.tty acids,volatiles,diterpenes,sterols,and phenolic substances,among *** f.r as the authors know,there are no papers published in the literature that have compiled such an extensive set of.inf.rmation about these chromatographic methods as *** the last 2 years,there has been a trend to develop analytical methods f.r ultra-perf.rmance liquid chromatography coupled with tandem mass spectrometry to conf.rm f.aud in cof.ee,due to high sensitivity and selectivity.

关键词： adulteration cof.ee chromatographic methods carbohydrates volatile substances

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Minimal Vasovagal Dysautonomia in Patients with Rare or Unique Syncope

引用

International Journal of.Clinical Medicine 2022年第7期13卷 262-275页

作者： Pedro Jiménez-Cohl Maximiliano Aspeé Magaly Sepúlveda Bladimir Lepe José Ignacio godoy Sof.a Jiménez-Castillo Campus San f.lipe (Chile) and Department of.Neurology and Autonomic Studies Universidad de Valparaíso Hospital Mili-tar de Santiago Santiago Chile Public Health Department Putaendo Chile Campus San f.lipe Universidad de Valparaíso San Felipe Chile Universidad de Los Andes Santiago Chile

Introduction: It is common to f.nd people sent to perf.rm a Head Up Tilt Test (HUT) who suf.ered a single syncope, or syncopes that occur during certain periods and never appear again. We wonder how these people are dif.erent f.om those who have never had syncope. Methods: We f.und 300 patients who suf.ered only one (unique) or a maximum of.5 vasovagal syncopes during their lif.. And their HUT was positive f.r vasovagal dysautonomia. We compared them, with 120 healthy volunteers who have never had syncope. We try to explain how some constitutional predisposing f.ctors act in these patients, and are associated with environmental triggers to precipitate the syncope. Results: We f.und dif.erences between cases and controls in predisposing f.ctors such as: heredity, joint hypermobility, baroref.ex f.ilure, venous compliance and some neurological diseases. Then an environmental f.ctor acts as a trigger f.r syncope: prolonged standing, stress, pain and emotions, dehydration, use of.certain drugs, abundant f.od. Conclusions: There are people with minimally expressed vasovagal dysautonomia who have an organic predisposition to present vasovagal syncopes (heredity, joint hypermobility, baroref.ex f.ilure, venous compliance, some neurological diseases, etc.). But this predisposition is not enough by itself.to produce syncopes. One or more environmental f.ctors must be added, acting as a trigger that would be the reason why these episodes are so inf.equent.

关键词： Dysautonomia Unique Vasovagal Syncope Head Up Tilt Test

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

应用突变体证明草酸在核盘菌对菜豆致病性中的作用

引用

农业科技译丛（湖南） 1991年第2期20卷 75-79页

作者： godoy,G 刘学端

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Characterization of.hepatitis B virus X gene quasispecies complexity in mono-inf.ction and hepatitis delta virus superinf.ction

引用

World Journal of.Gastroenterology 2019年第13期25卷 1566-1579页

作者： Cristina godoy David Tabernero Sara Sopena Josep Gregori Maria f.ancesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Raf.el Esteban Mar Riveiro-Barciela Maria Buti f.ancisco Rodríguez-f.ías Liver Pathology Unit Departments of Biochemistry and Microbiology Hospital Universitari Vall d’Hebron Universitat Autònoma de Barcelona Liver Unit Liver Disease Laboratory-Viral Hepatitis Vall d’Hebron Institut Recerca-Hospital Universitari Vall d'Hebron Universitat Autònoma de Barcelona Centro de Investigación Biomédica en Red de Enf.rmedades Hepáticas y Digestivas (CIBERehd) Instituto de Salud Carlos Ⅲ Roche Diagnostics SL Centro de Investigación Médica Aplicada (CIMA) Universidad de Navarra Liver Unit Department of Internal MedicineHospital Universitari Vall d’Hebron Universitat Autònoma de Barcelona (UAB)

Hepatitis delta virus(HDV) seems to strongly suppress hepatitis B virus(HBV)replication, although little is known about the mechanism of.this interaction. Both these viruses show a dynamic distribution of.mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach f.r analyzing the composition of.these mutant spectra. As the regulatory hepatitis B X protein(HBx) is essential f.r HBV replication, determination of.HBV X gene(HBX)quasispecies complexity in HBV/HDV inf.ction compared to HBV monoinf.ction may provide inf.rmation on the interactions between these two *** To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV mono-inf.cted *** Twenty-f.ur untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV inf.ction(CI, previously termed inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum sample f.om each patient was f.rst tested f.r HBV DNA *** HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-amplif.ed f.r subsequent next-generation sequencing(MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices(number of.haplotypes and number of.mutations), abundancebased indices(Hill numbers of.order 1 and 2), and f.nctional indices(mutation f.equency and nucleotide diversity). We also evaluated the pattern of.nucleotide changes to investigate which of.them could be the cause of.the quasispecies *** CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P f.cantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of.order 2 showed

关键词： Hepatitis B virus Hepatitis delta virus Hepatitis B X gene Next-generation sequencing Viral quasispecies Hepatitis B virus-hepatitis delta virus interaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agrobacterium-mediated transient transf.rmation of.Pentalinon andrieuxii Mü***.

引用

Advances in Bioscience and Biotechnology 2012年第3期3卷 256-258页

作者： Alejandro Yam-Puc Elide Aviles-Berzunza Manuel J.Chan-Bacab Luis Manuel Pena-Rodriguez Gregorio godoy-Hernandez Centro de Investigacion Cientif.ca de Yucatan A.C.Laboratorio de Quimica OrganicaUnidad de BiotecnologiaMeridaMexico Unidad de Bioquimica y Biologia Molecular MeridaMexico Departamento de Microbiologia Ambiental y Biotecnologia Universidad Autonoma de CampecheCampecheMexico

Sections of.hypocotyls, roots and leaves f.om Pentalinon andrieuxii plantlets were transiently transf.rmed with Agrobacterium tumef.ciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all inf.cted tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the f.rst report of.Pentalinon andrieuxii susceptibility to Agrobacterium tumef.ciens-mediated genetic transf.rmation.

关键词： β-Glucuronidase Agrobacterium tumef.ciens Kanamycin Apocynaceae

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Conservation and variability of.hepatitis B core at dif.erent chronic hepatitis stages

引用

World Journal of.Gastroenterology 2020年第20期26卷 2584-2598页

作者： Marcal Yll Maria f.ancesca Cortese Mercedes Guerrero-Murillo Gerard Orriols Josep Gregori Rosario Casillas Carolina González Sara Sopena Cristina godoy Marta Vila David Tabernero Josep Quer Ariadna Rando Rosa Lopez-Martinez Raf.el Esteban Mar Riveiro-Barciela Maria Buti f.ancisco Rodríguez-f.ías Liver Pathology Unit Departments of Biochemistry and MicrobiologyHospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Liver Unit Liver Disease Laboratory-Viral HepatitisVall d'Hebron Institut Recerca-Hospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Department of.Microbiology Hospital Universitari Vall d'HebronUniversitat Autònoma de BarcelonaBarcelona 08035Spain Centro de Investigación Biomédica en Red de Enf.rmedades Hepáticas y Digestivas Instituto de Salud Carlos ⅢMadrid 28029Spain Liver Unit Department of Internal MedicineHospital Universitari Vall d'HebronUniversitat Autónoma de BarcelonaBarcelona 08035Spain

BACKGROUND Since it is currently not possible to eradicate hepatitis B virus(HBV)inf.ction with existing treatments,research continues to uncover new therapeutic *** core protein,encoded by the HBV core gene(HBC),intervenes in both structural and f.nctional processes,and is a key protein in the HBV lif. *** this reason,both the protein and the gene could be valuable targets f.r new therapeutic and diagnostic ***,alterations in the protein sequence could serve as potential markers of.disease *** To detect,by next-generation sequencing,HBC hyper-conserved regions that could potentially be prognostic f.ctors and targets f.r new *** Thirty-eight of.45 patients with chronic HBV initially selected were included and grouped according to liver disease stage[chronic hepatitis B inf.ction without liver damage(CHB,n=16),liver cirrhosis(LC,n=5),and hepatocellular carcinoma(HCC,n=17)].HBV DNA was extracted f.om patients’plasma.A region between nucleotide(nt)1863 and 2483,which includes HBC,was amplif.ed and analyzed by next-generation sequencing(Illumina Mi Seq platf.rm).Sequences were genotyped by distance-based discriminant *** and intergroup nt and amino acid(aa)conservation was determined by sliding window *** presence of.nt insertion and deletions and/or aa substitutions in the dif.erent groups was determined by aligning the sequences with genotype-specif.c consensus *** Three nt(nt 1900-1929,2249-2284,2364-2398)and 2 aa(aa 117-120,159-167)hyper-conserved regions were shared by all the clinical *** groups showed a similar pattern of.conservation,except f.r f.ve nt regions(nt 1946-1992,2060-2095,2145-2175,2230-2250,2270-2293)and one aa region(aa 140-160),where CHB and LC,respectively,were less conserved(Pf.c conserved regions were also observed at both nt(2306-2334 in CHB and 1935-1976 and 2402-2435 in LC)and aa(between aa 98-103 in CHB and 28-30 and 5

关键词： Hepatitis B virus Hepatitis B core gene Next-generation sequencing Genetic conservation Amino acid substitution Gene therapy Small interf.ring RNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

风压下钢储罐屈曲荷载下限值计算的难点

引用

钢结构(中英文) 2010年第12期 86-87页

作者： Eduardo M.Sosa Luis A.godoy

介绍通用有限元代码编写的一种缺陷敏感壳单元,用于计算壳体屈曲荷载的下限值。风压下两种形状的钢储罐的稳定性较好,分别为:锥形顶式和顶部开口式。分析这两种钢储罐的一些几何关系,用于找出尺寸改变而引起的变化。折减能量法用于计算... 详细信息

介绍通用有限元代码编写的一种缺陷敏感壳单元,用于计算壳体屈曲荷载的下限值。风压下两种形状的钢储罐的稳定性较好,分别为:锥形顶式和顶部开口式。分析这两种钢储罐的一些几何关系,用于找出尺寸改变而引起的变化。折减能量法用于计算极限风压下钢储罐屈曲强度的下限值,而且将下限值与采用同一模型的静态非线性分析结果进行了比较。为优化原假设理论得到的结果,提出了实现折减能量法的另一个新思路。

关键词：屈曲非线性分析折减能量法储罐风压

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：