Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Mü***.

作     者：Alejandro Yam-Puc Elide Aviles-Berzunza Manuel J.Chan-Bacab Luis Manuel Pena-Rodriguez Gregorio Godoy-Hernandez 

作者机构：Centro de Investigacion Cientifica de YucatanA.C.Laboratorio de Quimica OrganicaUnidad de BiotecnologiaMeridaMexico Unidad de Bioquimica y Biologia MolecularMeridaMexico Departamento de Microbiologia Ambiental y BiotecnologiaUniversidad Autonoma de CampecheCampecheMexico 

出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)

年 卷 期：2012年第3卷第3期

页      面：256-258页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：financial support from the National Council for Science and Technology-Mexico(CONACYT)(Project No.59695-Z) 

主　　题：β-Glucuronidase Agrobacterium tumefaciens Kanamycin Apocynaceae 

摘      要：Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.