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Phyton-International Journal of Experimental Botany 2023年第9期92卷 2503-2518页

作者： joséAdrian Perez-Robles carlos Alberto Lecona-Guzmán Víctor Manuel Ruíz-Valdiviezo Joaquín Adolfo Montes-Molina Laboratorio de Biotecnología Vegetal Tecnológico Nacional de MexicoCampus Tuxtla GutiérrezTuxtla Gutiérrez29050Mexico

The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum *** with Fusarium oxysporum after the combined application of Beauveria bassiana and plant *** lycopersicum plantlets were transplanted 15 days after the *** days after transplanting,Beauveria bassiana spores were applied at a concentration of 1×10^(7)spores mL^(−1)onto soil(along with ***(N)and ***(H)leaf extracts)where *** plants were *** days after transplanting,spores of *** strain were applied at a concentration of 1×10^(6)spores mL^(−1)to soil where *** plants were *** development of *** plants was monitored for 114 days,whereby a randomized complete block treatment design was used,the roots of the plants were crushed with liquid nitrogen,after which UV/VIS spectrophotometry was used to determine protein concentration,as well as the activity ofβ-1,3-glucanases,peroxidases(POX,EC1.***.1.7),catalase and *** treatments combining *** and *** and *** extracts,had a significant difference in enzymatic activity levels,thus contributing to better defense mechanisms and a greater protection to *** plants in the presence of *** application of *** and plant extracts to the ground mitigated damage caused by *** on *** plants.

关键词： Glucanases chitinases peroxidases catalase defense mechanisms

Computer-Aided Diagnosis for Tuberculosis Classification with Water Strider Optimization Algorithm

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Computer Systems Science & Engineering 2023年第8期46卷 1337-1353页

作者： josé Escorcia-Gutierrez Roosvel Soto-Diaz Natasha Madera carlos Soto Francisco Burgos-Florez Alexander Rodríguez Romany F.Mansour Department of Computational Science and Electronic Universidad de la CostaCUCBarranquilla080002Colombia Biomedical Engineering Program Universidad Simón BolívarBarranquilla080002Colombia Mechatronics Engineering Program Universidad Simón BolívarBarranquilla080002Colombia Department of Medicine Universidad del NorteBarranquilla081007Colombia Department of Mathematics Faculty of ScienceNew Valley UniversityEl-Kharga72511Egypt

Computer-aided diagnosis(CAD)models exploit artificial intelligence(AI)for chest X-ray(CXR)examination to identify the presence of tuberculosis(TB)and can improve the feasibility and performance of CXR for TB screening and *** the same time,CXR interpretation is a time-consuming and subjective ***,high resemblance among the radiological patterns of TB and other lung diseases can result in ***,computer-aided diagnosis(CAD)models using machine learning(ML)and deep learning(DL)can be designed for screening TB *** this motivation,this article develops a Water Strider Optimization with Deep Transfer Learning Enabled Tuberculosis Classification(WSODTL-TBC)model on Chest X-rays(CXR).The presented WSODTL-TBC model aims to detect and classify TB on CXR ***,the WSODTL-TBC model undergoes image filtering techniques to discard the noise content and U-Net-based image ***,a pre-trained residual network with a two-dimensional convolutional neural network(2D-CNN)model is applied to extract feature *** addition,the WSO algorithm with long short-term memory(LSTM)model was employed for identifying and classifying TB,where the WSO algorithm is applied as a hyperparameter optimizer of the LSTM methodology,showing the novelty of the *** performance validation of the presented WSODTL-TBC model is carried out on the benchmark dataset,and the outcomes were investigated in many *** experimental development pointed out the betterment of the WSODTL-TBC model over existing algorithms.

关键词： Computer-aided diagnosis water strider optimization deep learning chest x-rays transfer learning

Effluent Zero Release Concept-The Brazilian Experience

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过程工程学报 2006年第4期6卷 691-696页

作者： josé carlos Mierzwa Sandra Mara Garcia Bello Ivanildo Hespanhol Hydraulic and Sanitation Eng.Dept. Univ.Sao PauloAv.Prof.Almeida Prado83 SoPauloBrazil CEP:05508-900 Navy Technologic Center in Sao Paulo Av.Prof.Lineu Prestes2468 SoPauloBrazilCEP:05508-900

Water scarcity is pushing the government,industries and researchers to the development of new strategies for water and wastewater *** approach aimed at the optimization of the water use and minimization of effluent generation was developed at the Centro Experimental ARAMAR(CEA),a nuclear research facility,located in the State of Sao Paulo,*** scale tests followed by a pilot plant treating effluents from some nuclear research facilities have shown the results leading to the conclusion that the effluent zero release concept is *** on the gathered data,a project of an integrated effluent treatment system focusing on water recovery and environmental effluent release reduction has been developed.

关键词： effluent wastewater treatment zero release

Phytochemical screening and antioxidant activity of ethanol extract of Tithonia diversifolia(Hemsl)*** dry flowers

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Asian Pacific Journal of Tropical Biomedicine 2014年第9期4卷 740-742页

作者： Robson Miranda da Gama Marcelo Guimares Luiz carlos de Abreu josé Armando-Junior Laboratorio de Pesquisa do Curso de Farmacia Faculdade de Medicina do ABC.Avenida Principe de Gales821Vila Principe de Gales09060-870Santo AndreSPBrasil Departamento de Saude Materno-Infantil Faculdade de Saude Publica.Universidade de Sao PauloBrasil

Objective:To evaluate the antioxidant activity of extracts of dried flowers of Tithonia diversifolia(Hemsl) ***(***) dry flower-a slirubby plant belonging to the Asteraceac family and very common in Brazil,providing data to help prevent premature aging ***:The tests of phytochemical screening included total phenols,tannins,flavonoids,alkaloids and *** active antioxidant was determined by 2,2-diphenyl-1-picrylhydrazyl ***:The phytoehemical screening of *** dry flowers revealed the presence of phenolic compounds(tannins,flavonoids and total phenolsi,while alkaloids and saponins were not *** IC_(50) values showed a strong antioxidant activity of the plant ***:Therefore,this study suggests the possibility of using dry flowers extracts of *** for the prevention of cell aging,as was shown to have significant antioxidant activity.

关键词： Antioxidant activity Phytochemical screening Tithonia diversifolia(Hemsl) A.Gray

A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation

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BIOCELL 2021年第2期45卷 331-337页

作者： SERGIO GASTÓN CASPE josÉLUIS KONRAD DADIN PRANDO MOORE JUAN MANUEL SALA LILIAN LISCHINSKY carlos MANUEL CAMPERO CLAUDIO GUSTAVO BARBEITO Laboratory of Pathology Experimental Station MercedesInstituto Nacional de Tecnología AgropecuariaCorrientes3470Argentina Laboratory of Theriogenology School of Veterinary SciencesUniversidad Nacional del NordesteCorrientesW3402BKGArgentina Animal Health Group Experimental Station BalcarceInstituto Nacional de Tecnología AgropecuariaBalcarceB7620EMAArgentina Laboratory of Histology and Embryology School of Veterinary SciencesUniversidad Nacional de La PlataLa Plata1900Argentina

Water buffalo(Bubalus bubalis)and domestic cattle(Bos taurus)are closely related ***,embryo transfer interspecies has been attempted without any *** failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal *** patterns have been studied in different species of ruminants;however,in ***,only the binucleated cells(BNC)have been *** glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry(LHC).The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by *** samples of placenta and uterus from pregnant Mediterranean female water buffaloes(Buf1)and Angus cows(Bov1)were *** animals were euthanized at 98 days of *** was carried out using twelve lectins(Con A,LCA,PSA,sWGA,PHA-e,SBA,UEA-1,WGA,RCA-1,PNA,DBA,BSA-1).The intensity of lectin binding was semiquantitatively scored using a scale of 0(negative)to 3(strongly positive).Difference between species was found in trophoblast layer by PSA,SWGA,PNA and BSA-1,in BNC,and in the mononuclear cells by LCA,PSA,PHA-e,DBA,BSA-1,*** utero,differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1,and in the cytoplasm of those glandular epithelial cells by PHA-e,BSA-1,WGA,and *** both species,BNC presented a strong positive reaction with DBA and SBA,a moderate response by LCA,PHA-e,BSA-1 and PNA lectin,and a low reaction by PSA,UEA-1,SWGA,WGA,Con A and *** results found in this study suggest that although both species are closely related,glycosylation patterns in the placenta and uterus are different,thus providing a possible reason for embryo transfer not being possible between these species.

关键词： Lectin-histochemistry Water buffalo Placenta Glycosylation pattern

Evaluation of the iridocorneal angle with accommodation using optical coherence tomography

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International Journal of Ophthalmology(English edition) 2017年第10期10卷 1614-1616页

作者： Daniel Monsálvez-Romín Antonio Del águila-carrasco Teresa Ferrer-Blasco josé J.Esteve-Taboada Robert Montés-Micó Optics and Optometry and Vision Sciences Department University of Valencia

The changes in the iridocorneal angle structure during accommodation are assessed by means of anterior segment optical coherence *** right eyes were included in the *** device used for the measurement was the Visante？omni *** stimuli were set up at different vergences（0.0 D,-1.5 D,and-3.0 D）.The angle opening distance 500 and 750,the trabecular iris space area 500 and 750,and the scleral spur angle parameters were assessed at the nasal and temporal *** results in the iridotrabecular angle comparing the three accommodative states of the eye did not yield any statistically significant difference at nasal or temporal angle *** light of our results and in the conditions of our study,the structures of the iridocorneal angle are not significantly changed with accommodation.

关键词： iridocorneal angle accommodation optical coherence tomography anterior segment

New Technique to Create a Suspension Containing Bacteriophages and How It Can Be Used to Control Cabbage Leaf Spot Caused by <i>Xanthomonas campestris</i>PV. <i>campestris</i>

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Agricultural Sciences 2014年第4期5卷 286-297页

作者： Igor Villela Marroni josé carlos Germani Department of Basic Health Sciences Rua Sarmento Leite Universidade Federal do Rio Grande do Sul Porto Alegre Brazil

The purpose of the present study was to develop a new method for producing and identifying a bacteriophage suspension and research its potential against plant bacteria. The suspension obtained with the new technique was effective in controlling Xanthomonas campestris PV. campestris both in vitro and in vivo. A difference was observed between the suspension with pathogen (PLP) and crude suspension with lesioned plant without the pathogen originated from it (PLN). Clear zones were observed at 48 h/28°C in both (PLS) suspension added saccharose and (PLP). Although PLP and PLS treatments showed no statistical difference in CFU values (5 × 105 and 1 × 105, respectively), they differed from the crude suspension (PLN) and the control. Then, in vivo assessment of suspensions with clear zones in the dishes was performed. In vivo (PLPS) and (PLPP) treatments consisting of inoculum were added to the crude suspensions (PLS and PLP) to increase bacteriophages. A decrease in the disease severity index compared to the control was observed, showing that treatment with the phage suspension protects the plant. The lowest disease indexes were reported with (PLP) and (PLS), with values of 1.46 and 1.40, respectively. The highest averages in dry matter (5.1 inboth treatments) were obtained with (PLP) and (PLS) treatments.

关键词： Biocontrol Virus Bacteria Disease Index

Assessment of Genetic Stability Among In Vitro Plants of Arachis retusa Using RAPD and AFLP Markers for Germplasm Preservation

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Journal of Integrative Plant Biology 2007年第3期49卷 307-312页

作者： Rachel Fatima Gagliardi Luiz Ricardo Hanai Georgia Pacheco carlos Alberto Oliveira Leonardo Alves Carneiro josé Francisco Montenegro Valls Elisabeth Mansur Maria Lucia Carneiro Vieira Laboratório de Micropropagacao e Transformacao de Plantas Universidade do Estado do Rio de Janeiro postal code 20550-013 Maracana Rio de Janeiro Brazil Departamento de Genética - Escola Superior de Agricultura Luiz de Queiroz Universidade de Sao Paulo postal code 13418-900 Piracicaba Brazil Embrapa Recursos Genéticos e Biotecnologia postal code 70770-901 Brasilia Brazil

Arachis retusa Krapov. et W. C. Gregory et Valls is endemic in the West-central region of Brazil, occurring In areas endangered by human actions. The establishment of in vitro preservation methods for wild species of Arachis is an alternative to seed banks for germplasm storage, multiplication and distribution. The risk of genetic changes Induced by tissue culture and the monitoring of the genetic stability of the biological material before, during and after storage must be considered In the context of conservation. Random amplified polymorphlc ONA （RAPO） and amplified fragment length polymorphism （AFLP） fingerprinting were used to evaluate the genetic stability of in vitro plants originated from cotyledons and embryo axes of A. retusa. Cotyledons originated shoots through direct organogenesls and embryo axes displayed muItishoot formation Induced by 110 mmol/L and 8.8 mmol/L BAP, respectively. Ninety genomlc regions （loci） generated from RAPO and 372 from AFLP analyses were evaluated. All amplified fragments detected by both techniques in plants derived from the two explant types were monomorphic. The results Indicate that the recovered shoots are genetically stable at the assessed genomic regions.

关键词： amplified fragment length polymorphism Arachis retusa in vitro preservation micropropagation random amplifiedpolymorphic DNA somaclonal variation

Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility

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Asian Journal of Andrology 2004年第1期6卷 59-65页

作者： Nicolás Garrido Marcos Meseguer carlos Simon Antonio Pellicer josé Remohi Instituto Valenciano de Infertilidad Valencia Valencia 46015 Spain Fundación Instituto Valenciano de Infertilidad Valencia SpainInstituto Valenciano de Infertilidad Valencia Valencia 46015 Spain Fundación Instituto Valenciano de Infertilidad Valencia SpainInstituto Valenciano de Infertilidad Valencia Valencia 46015 Spain Department of Pediatrics Obstetrics and Gynecology Valencia University School of Medicine Valencia SpainInstituto Valenciano de Infertilidad Valencia Valencia 46015 SpainDepartment of Pediatrics Obstetrics and Gynecology Valencia University School of Medicine Valencia SpainDepartment of Obstetrics and Gynecology Hospital Dr. Peset Valencia SpainInstituto Valenciano de Infertilidad Valencia Valencia 46015 SpainDepartment of Obstetrics and Gynecology Hospital Dr. Peset Valencia Spain

Oxidative stress is a common condition suffered by biological systems in aerobic conditions. Human semen also has its own molecular guard against the free radicals created by normal respiratory process or from immune reactions. The equilibrium of the creation and scavenging of free radicals is mandatory in the spermatozoa to fertilize and initiate a full-term pregnancy. The paper is a systematic review of publications that evaluate oxidative stress in semen. The Cochrane Library, Medline (1966-2003), Embase (1988-2003), SciSearch (1981-2003) and the conference papers were searched. When sperm samples from fertile and infertile males were analyzed, some of the mechanisms that determine the oxidative stress level were found to be impaired while others were unaltered. In conclusion, the literature as a whole provides contradictory findings and it is necessary to carry out more work to identify all the enzymatic and non-enzymatic systems involved in oxidative stress in the ejaculate, in order to develop new diagnostic systems and therapeutic strategies for combating detrimental free radical imbalance in the semen.

关键词： oxidative stress antioxidants semen spermatozoa male fertility

Testing for Deterministic Components in Vector Seasonal Time Series

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Open Journal of Statistics 2011年第3期1卷 145-150页

作者： josé Luis Gallego carlos Díaz 不详

Certain locally optimal tests for deterministic components in vector time series have associated sampling distributions determined by a linear combination of Beta variates. Such distributions are nonstandard and must be tabulated by Monte Carlo simulation. In this paper, we provide closed form expressions for the mean and variance of several multivariate test statistics, moments that can be used to approximate unknown distributions. In particular, we find that the two-moment Inverse Gaussian approximation provides a simple and fast method to compute accurate quantiles and p-values in small and asymptotic samples. To illustrate the scope of this approximation we review some standard tests for deterministic trends and/or seasonal patterns in VARIMA and structural time series models.

关键词： Vector Time Series Deterministic Components Parametric Stability Non-Invertibility Unit Roots