Effects of Plant Extracts and Beauveria bassiana on the Activity of Defense-Related Enzymes in Solanum lycopersicum L.during Interaction with Fusarium oxysporum f.sp.lycopersici
作者机构：Laboratorio de Biotecnología VegetalTecnológico Nacional de MexicoCampus Tuxtla GutiérrezTuxtla Gutiérrez29050Mexico
出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)
年 卷 期：2023年第92卷第9期
页 面：2503-2518页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护]
基 金：funded by the Tecnológico Nacional de México(TECNM):Project No.6602.18-P.
主 题：Glucanases chitinases peroxidases catalase defense mechanisms
摘 要：The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum L.infected with Fusarium oxysporum after the combined application of Beauveria bassiana and plant extracts.Solanum lycopersicum plantlets were transplanted 15 days after the emergency.Five days after transplanting,Beauveria bassiana spores were applied at a concentration of 1×10^(7)spores mL^(−1)onto soil(along with A.indica(N)and P.auritum(H)leaf extracts)where S.lycopersicum plants were planted.Eight days after transplanting,spores of F.oxysporum strain were applied at a concentration of 1×10^(6)spores mL^(−1)to soil where S.lycopersicum plants were growing.The development of S.lycopersicum plants was monitored for 114 days,whereby a randomized complete block treatment design was used,the roots of the plants were crushed with liquid nitrogen,after which UV/VIS spectrophotometry was used to determine protein concentration,as well as the activity ofβ-1,3-glucanases,peroxidases(POX,EC1.***.1.7),catalase and chitinases.The treatments combining B.bassiana and A.indica and P.auritum extracts,had a significant difference in enzymatic activity levels,thus contributing to better defense mechanisms and a greater protection to S.lycopersicum plants in the presence of F.oxysporum.The application of B.bassiana and plant extracts to the ground mitigated damage caused by F.oxysporum on S.lycopersicum plants.
维普期刊数据库