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Association Analysis of the Amino Acid Contents in Rice

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Journal of Integrative Plant Biology 2009年第12期51卷 1126-1137页

作者： Weiguo Zhao Eun-Jin Park jong-wook chung Yong-Jin Park III-Min chung Joung-Kuk Ahn Gwang-Ho Kim Department of Appfied Bioscience Konkuk University Seou1143-701 Korea Seficultural Research Institute Chinese Academy of Agricultural Sciences Jiangsu University of Science and Technology Zhenjiang 212018 China Department of Plant Resources Coflege of Industrial Science Kongju National University Yesan 340-802 Korea

The main objective of the present study was to identify simple sequence repeat （SSR） markers associated with the amino acid content of rice （Oryza sativa L.）. SSR markers were selected by prescreening for the relationship to amino acid content. Eighty-four rice landrace accessions from Korea were evaluated for 16 kinds of amino acids in brown rice and ganotyped with 25 SSR markers. Analysis of population structure revealed four subgroups in the population. Linkage disequilibrium （LD） patterns and distributions are of fundamental importance for genome-wide mapping associations. The mean r^2 value for all intrachromosomal loci pairs was 0.033. LD between linked markers decreased with distance. Marker-trait associations were investigated using the unified mixed-model approach, considering both population structure （Q） and kinship （K）. A total of 42 marker-trait associations with amino acids （P 〈 0.05） were identified using 15 different SSR markers covering three chromosomes and explaining more than 40% of the total variation. These results suggest that association analysis in rice is a viable alternative to quantitative trait loci mapping and should help rice breeders develop strategies for improving rice quality.

关键词： Amino acid content association mapping linkage disequilibrium population structure simple sequence repeats.

Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

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Journal of Integrative Plant Biology 2011年第1期53卷 63-73页

作者： Kyaw Thu Moe jong-wook chung Young-Il Cho Jung-Kyung Moon Ja-Hwan Ku Jin-Kyo Jung Jungran Lee Yong-Jin Park Department of Plant Resources College of Industrial SciencesKongju National University The Rural Development Administration(RDA) Legume Bio-Resource Center of Green Manure(LBRCGM) Kongju National University

Mungbean （Vigna radiata （L.） Wilczek） is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs （〉_500 bp） with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat （SSR） motifs was identified in Jangan （1 630） compared with that of Sunhwa （1 334）. A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms （SNPs） and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was -860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.

关键词： Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean SNPs

Development of a Core Set from a Large Rice Collection using a Modified Heuristic Algorithm to Retain Maximum Diversity

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Journal of Integrative Plant Biology 2009年第12期51卷 1116-1125页

作者： Hun-Ki chung Kyu-Won Kim jong-wook chung Jung-Ro Lee Sok-Young Lee Anupam Dixit Hee-Kyoung Kang Weiguo Zhao Kenneth L. McNally Ruraidh S. Hamilton Jae-Gyun Gwag Yong-Jin Park National Agrobiodiversity Center National Academy of Agricultural Science Suwon 441-707 Korea Department of Plant Resources College of Industrial Sciences Kongju National University Yesan 340-802 Korea Rice DNA and Quality Testing Laboratory Basmati Export Development Foundation SVBP University of Agriculture and Technology Meerut 250110 India Department of Horticultural Science College of Industrial Science Kongju National University Yesan 340-802 Korea jiangsu University of Science and Technology Seficultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang 212018 China Genetic Resources Center International Rice Research Institute Metro Manila 1301 Philippines

A new heuristic approach was undertaken for the establishment of a core set for the diversity research of rice. As a result, 107 entries were selected from the 10 368 characterized accessions. The core set derived using this new approach provided a good representation of the characterized accessions present in the entire collection. No significant differences for the mean, range, standard deviation and coefficient of variation of each trait were observed between the core and existing collections. We also compared the diversity of core sets established using this Heuristic Core Collection （HCC） approach with those of core sets established using the conventional clustering methods. This modified heuristic algorithm can also be used to select genotype data with allelic richness and reduced redundancy, and to facilitate management and use of large collections of plant genetic resources in a more efficient way.

关键词： core collection genetic diversity heuristic core collection Oryza sativa sampling strategy.

Analysis of genetic relationships of mulberry （Morus L.） germplasm using sequence-related amplified polymorphism （SRAP） markers

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Analysis of genetic relationships of mulberry （Morus L.） g...

Crystallographically vacancy‐induced MOF nanosheet as rational single‐atom support for accelerating CO_(2)electroreduction to CO

中国蚕学会第六届青年学术研讨会

作者： jong-wook chung Yong-jin Park Department of Molecular Biotechnology Konkuk University Department of Plant Resources College of Industrial ScienceKongju National University

Sequence-related amplified polymorphism（SRAP） is a novel molecular marker technique designed to amplify open reading frames（ORFs） . Here,for the first time,SRAP was used to identify mulberry germplasm. Twenty-three mulberry accessions were screened using SRAP techniques,with 12 primer combinations selected for their reproducibility and polymorphism. Out of 83 PCR fragments scored,59（71.1%） were polymorphic,with an average of 4.9 polymorphic bands and 6.9 bands per primer combination. Mean gene diversity and PIC were 0.1611 and 0.1353,respectively. The genetic similarity coefficient ranged from 0.6905 to 0.9524,with an average of 0.8330. The phylogenetic tree was obtained using the UPGMA method using the total number of amplified SRAP fragments. The results from cluster analysis were in general agreement with our morphologic classification.

关键词： mulberry molecular marker genetic diversity SRAP

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Carbon Energy 2024年第8期6卷 49-62页

作者： Jin Hyuk Cho Joonhee Ma Chaehyeon Lee Jin wook Lim Youngho Kim Ho Yeon Jang Jaehyun Kim Myung‐gi Seo Youngheon Choi Youn Jeong Jang Sang Hyun Ahn Ho Won Jang Seoin Back jong‐Lam Lee Soo Young Kim Department of Materials Science and Engineering Korea UniversitySeoulRepublic of Korea Department of Chemical and Biomolecular Engineering Sogang UniversitySeoulRepublic of Korea Department of Materials Science and Engineering Pohang University of Science and Technology(POSTECH)PohangRepublic of Korea Department of Materials Science and Engineering Research Institute of Advanced MaterialsSeoul National UniversitySeoulRepublic of Korea Lotte Chemical R&D Center DaejeonRepublic of Korea Department of Chemical Engineering Hanyang UniversitySeoulRepublic of Korea School of Chemical Engineering and Material Science Chung‐Ang UniversitySeoulRepublic of Korea

To attain a circular carbon economy and resolve CO_(2)electroreduction technology obstacles,single‐atom catalysts(SACs)have emerged as a logical option for electrocatalysis because of their extraordinary catalytic *** SACs,metal–organic frameworks(MOFs)have been recognized as promising support materials because of their exceptional ability to prevent metal *** study shows that atomically dispersed Ni single atoms on a precisely engineered MOF nanosheet display a high Faradaic efficiency of approximately 100%for CO formation in H‐cell and three‐compartment microfluidic flow‐cell reactors and an excellent turnover frequency of 23,699 h−1,validating their intrinsic catalytic *** results suggest that crystallographic variations affect the abundant vacancy sites on the MOF nanosheets,which are linked to the evaporation of Zn‐containing organic linkers during ***,using X‐ray absorption spectroscopy and density functional theory calculations,a comprehensive investigation of the unsaturated atomic coordination environments and the underlying mechanism involving CO^(*)preadsorbed sites as initial states was possible and provided valuable insights.

关键词： 2‐dimensional material carbon dioxide reduction metal-organic frameworks single‐atom catalysts vacancy sites

Optimal sequential therapy using tyrosine kinase inhibitors as the first-line treatment in patients with metastatic renal cell carcinoma: A nationwide multicenter study

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Asian Journal of Urology 2024年第3期11卷 450-459页

作者： Jung Ki Jo Seong Il Seo MinYong Kang Jinsoo chung Cheol Kwak Sung-Hoo Hong Cheryn Song Jae Young Park Chang wook Jeong Seok Hwan Choi Sung Han Kim Eu Chang Hwang Chan Ho Lee Hakmin Lee Department of Urology Medical and Digital EngineeringCollege of MedicineHanyang UniversitySeoulRepublic of Korea Department of Urology Samsung Medical CenterSungkyunkwan University School of MedicineSeoulRepublic of Korea Department of Urology National Cancer CenterGoyangRepublic of Korea Department of Urology Seoul National University HospitalSeoulRepublic of Korea Department of Urology Kangnam St Mary’s HospitalSeoulRepublic of Korea Department of Urology Asan Medical CenterSeoulRepublic of Korea Department of Urology Korea University Ansan HospitalAnsanRepublic of Korea Department of Urology Kyungpook National University HospitalDaeguRepublic of Korea Department of Urology Chonnam National University Hwasun HospitalHwasunRepublic of Korea Department of Urology Inje University Busan Paik HospitalBusanRepublic of Korea Department of Urology Seoul National University Bundang HospitalSeongnamRepublic of Korea

Objective:The purpose of the study was to identify the best sequence of therapy beginning with a tyrosine kinase inhibitor(TKI)as the first-line therapy for patients with metastatic renal cell carcinoma(mRCC)in terms of overall survival(OS),progression-free survival(PFS),and rates of discontinuation and adverse effects during the treatment ***:This is a retrospective,nationwide multicenter study of patients with mRCC after diagnosis at 10 different tertiary medical centers in Korea from January 1992 to December *** focused on patients at either“favorable”or“intermediate”risk according to the International mRCC Database Consortium criteria,and they were followed up(median 335 days).Finally,a total of 1409 patients were selected as the study *** generated a Cox proportional hazard model adjusted for covariates,and the different therapy schemes were statistically tested in terms of OS as well as *** addition,frequencies of discontinuation and adverse events were compared among the therapy ***:Of the primary patterns of treatment sequences(24 sequences),“sunitinib epazopanib”and“sunitinibeeverolimuseimmunotherapy”showed the most beneficial results in both OS and PFS with significantly lower hazards than“sunitinib”,which is the most commonly treated agent in *** that the“TKIeTKI”structure showed relatively higher discontinuation rates with higher adverse effects,the overall beneficial sequence would be“sunitinibeeverolimuseimmunotherapy”.Conclusion:Among several sequential therapy starting with TKIs,“sunitinibeeverolimuse immunotherapy”was found to be the best scheme for mRCC patients with“favorable”or“intermediate”risks.

关键词： Tyrosinekinase inhibitor Metastaticrenalcell carcinoma Overall survival Progression-free survival

Enhancing polyphenol extraction from unripe apples by carbohydrate-hydrolyzing enzymes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第12期10卷 912-919页

作者： In-wook HWANG Shin-Kyo chung Department of Food Science and Technology Kyungpook National University Daegu 702-701 Korea

The effects of process variables such as enzyme types, enzyme ratio, reaction temperature, pH, time, and ethanol concentration on the extraction of unripe apple polyphenol were investigated. The results indicated that Viscozyme L had the strongest effect on polyphenols extraction and was selected to study the polyphenol composition. The ratio of enzyme （Viscozyme L） to sobstrate （2 fungal beta-glucanase units （FBG）） at 0.02, reaction at pH 3.7, 50℃ for 12 h, and ethanol concentration of 70% were chosen as the most favorable extraction condition. Total phenolic content （TPC）, reducing sugar content （RSC）, and extraction yield increased by about 3, 1.5, and 2 times, respectively, compared with control. The contents ofp-coumaric acid, ferulic acid, and caffeic acid increased to 8, 4, and 32 times, respectively. The enzyme-aided polyphenol extraction process from unripe apples might be applied to food industry for enhancing bioactive compound production.

关键词： Carbohvdrate-hvdrolvzim enzvmes. Unrioe aonles. Polvnhenol extraction. Caffeic acid

Low skeletal muscle mass is associated with non-alcoholic fatty liver disease in Korean adults： the Fifth Korea National Health and Nutrition Examination Survey

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Hepatobiliary & Pancreatic Diseases International 2016年第1期15卷 39-47页

作者： hee yeon kim chang wook kim chung-hwa park jong young choi kyungdo han anwar t merchant yong-moon park division of hepatology department of internal medicinecollege of medicinethe catholic university of korea222Banpo-daeroSeocho-guSeou1 06591Republic of Korea department of biostatistics college of medicinethe catholic university of korea222Banpo-daeroSeocho-guSeou1 06591Republic of Korea department of epidemiology and biostatistics arnold school of public healthuniversity of south carolinaColumbiaSC 29208USA epidemiology branch national institute of environmental health sciencesnational institutes of healthdepartment of health and human servicesResearch Triangle ParkNC 27709USA

BACKGROUND： Sarcopenia and non-alcoholic fatty liver dis- ease （NAFLD） share similar pathophysiological mechanisms, and the relationship between sarcopenia and NAFLD has been recently investigated. The study investigated whether low skel- etal muscle mass is differentially associated with NAFLD by gender in Korean adults. METHODS： We conducted a cross-sectional analysis of the data from the Fifth Korea National Health and Nutrition Examination Survey. The skeletal muscle index （SMI） was obtained by the appendicular skeletal muscle mass divided by the weight. NAFLD was defined as a fatty liver index （FLI）〉60 in the absence of other chronic liver disease. RESULTS： Among the included subjects, 18.3% （SE： 1.4%） in men and 7.0% （SE： 0.7%） in women were classified as having FLI-defined NAFLD. Most of the risk factors for FLI-defined NAFLD showed a significant negative correlation with the SMI in both genders. Multiple logistic regression analysis showed that low SMI was associated with FLI-defined NAFLD, inde- pendent of other metabolic and lifestyle parameters in both genders [males： odds ratio （OR）=1.35; 95% confidence inter- val （CI）： 1.17-1.54; females： OR=1.36; 95% CI： 1.18-1.55]. The magnitude of the association between FLI-defined NAFLD and low SMI was higher in middle aged to elderly males （OR-1.50; 95% CI： 1.22-1.84） than in males less than 45 years of age （OR=1.25; 95% CI： 1.02-1.52） and in premenopausal females （OR=l.50; 95% CI： 1.12-2.03） than in postmenopausal females （OR-1.36; 95% CI： 1.20-1.54）.CONCLUSIONS： Low SMI is associated with the risk of FLI- defined NAFLD independent of other well-known metabolic risk factors in both genders. This association may differ ac- cording to age group or menopausal status. Further studies are warranted to confirm this relationship.

关键词： Korea National Health and Nutrition Examination Survey non-alcoholic fatty liver disease sarcopenia skeletal muscle

Suppression of laser-induced choroidal neovascularization by intravitreal injection of tristetraprolin

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International Journal of Ophthalmology(English edition) 2014年第6期7卷 952-958页

作者： Yong Wun Cho Yong Seop Han In Young chung Seong Jae Kim Seong wook Seo Ji Myong Yoo jong Moon Park Department of Ophthalmology Gyeongsang National University School of Medicine Gyeongsang Institute of Health Science Gyeongsang National University

AIM: To examine the effect of intravitreal adenoviral vector-mediated tristetraprolin(Ad-TTP) on VEGF m RNA expression in a rat model of laser-induced choroidal ***: Ad-TTP was prepared using a commercial kit. Retinal laser-induced photocoagulation(10 spots per eye) was performed on rats in this experimental choroidal neovascularization(CNV) model. Rats were divided into four groups: control(single intravitreal injection of balanced salt solution, n =10), laser-induced CNV(photocoagulation only, n =20), laser-induced CNV plus Ad-TTP injection(photocoagulation plus a single intravitreal Ad-TTP injection, n =20) and Ad-TTP injection only(n =10). Changes in choroidal morphology were evaluated in ten rats in the laser only and the laser plus Ad-TTP groups. Two weeks after laser injury, the size of CNV was calculated by perfusion with high-molecular-weight fluorescein isothiocyanate(FITC)-dextran. VEGF m RNA expression in retina-choroid tissue from ten rats in each group was measured by reverse transcription polymerase chain reaction(RT-PCR). RESULTS: Two weeks after treatment, the area of laser-induced CNV was reduced by approximately 60% in the rats given the Ad-TTP injection compared with that in the laser-only group. There was a tendency toward decreased VEGF m RNA expression in the Ad-TTP injection ***: A single intravitreal injection of Ad-TTP significantly suppressed CNV size in this experimental laser-induced CNV model. Ad-TTP injection also decreased VEGF m RNA expression compared with that inthe laser-induced CNV group. The present study is meaningful as the first study to investigate the effect of tristetraprolin delivered via intravitreal injection.

关键词： laser-induced choroidal neovascularization tristetraprolin adenoviral vectors