Analysis of genetic relationships of mulberry （Morus L.） germplasm using sequence-related amplified polymorphism （SRAP） markers
作者单位：Department of Molecular BiotechnologyKonkuk University Department of Plant ResourcesCollege of Industrial ScienceKongju National University
会议名称：《中国蚕学会第六届青年学术研讨会》
会议日期：2009年
学科分类：090504[农学-特种经济动物饲养（含：蚕、蜂等）] 0905[农学-畜牧学] 09[农学]
基 金：supported by the 2008 KU Brain Pool of Konkuk University, National Infrastructure and Condition Platform Programs for Science and Technology Development, China (20060400926) Jiangsu (0602004C) Postdoctoral Science Foundation Project Public Industry (Agriculture) Specific Research Program (nyhyzx07-020)
关 键 词：mulberry molecular marker genetic diversity SRAP
摘 要：Sequence-related amplified polymorphism（SRAP） is a novel molecular marker technique designed to amplify open reading frames（ORFs） . Here,for the first time,SRAP was used to identify mulberry germplasm. Twenty-three mulberry accessions were screened using SRAP techniques,with 12 primer combinations selected for their reproducibility and polymorphism. Out of 83 PCR fragments scored,59（71.1%） were polymorphic,with an average of 4.9 polymorphic bands and 6.9 bands per primer combination. Mean gene diversity and PIC were 0.1611 and 0.1353,respectively. The genetic similarity coefficient ranged from 0.6905 to 0.9524,with an average of 0.8330. The phylogenetic tree was obtained using the UPGMA method using the total number of amplified SRAP fragments. The results from cluster analysis were in general agreement with our morphologic classification.