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Agmatine reduces nitric oxide synthase expression and peroxynitrite formation in the cerebral cortex in a rat model of transient global cerebral ischemia

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Neural Regeneration Research 2010年第23期5卷 1773-1781页

作者： Chin Hee Mun Won Taek lee Kyung Ah Park jong eun lee Department of Anatomy Yonsei University College of Medicine Brain Korea 21 Project for Medical Science Yonsei University College of Medicine

Agmatine, an analog of L-arginine, is an endogenous substance synthesized by arginine decarboxylase, which has been shown to possess neuroprotective effects following brain ischemia. Nitric oxide is generated by sequential oxidation of the guanidinium group in L-arginine, and agmatine might protect the brain from ischemic injury by interfering with nitric oxide signaling. This study investigated the effects of agmatine on cerebral cortex neuronal injury following transient global cerebral ischemia and also detected nitric oxide synthase expression and peroxynitrite formation. Results demonstrated that intraperitoneal injection of agmatine in global cerebral ischemia/reperfusion alleviated ischemia/reperfusion-induced cerebral cortical cortex neuronal injury and cellular apoptosis, decreased neuronal and inducible nitric oxide synthase expression at 24, 48, and 72 hours following global cerebral ischemia and reperfusion, and greatly inhibited nitrotyrosine levels, which reflect the amount of peroxynitrite formed. These findings indicated that agmatine alleviates cerebral cortex neuronal injury following global cerebral ischemia and decreases nitric oxide synthase expression and peroxynitrite formation following ischemia/repeffusion.

关键词： agmatine global cerebral ischemia cerebral cortex nitric oxide nitric oxide synthase neuroprotection

Acute respiratory distress syndrome and severe pneumonitis after atezolizumab plus bevacizumab for hepatocellular carcinoma treatment:A case report

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World Journal of Gastrointestinal Oncology 2023年第5期15卷 892-901页

作者： Su Hyeon Cho Ga Ram You Chan Park Sang-Geon Cho jong eun lee Sung Kyu Choi Sung Bum Cho Jae Hyun Yoon Department of Gastroenterology and Hepatology Chonnam National University Hospital and Medical SchoolGwangju 61469South Korea Department of Gastroenterology and Hepatology Hwasun Chonnam National University Hospital and Medical SchoolHwasun 58128South Korea Department of Radiology Chonnam National University Hospital and Medical SchoolGwangju 61469South Korea Department of Nuclear Medicine Chonnam National University HospitalHwasun 58128South Korea

BACKGROUND Hepatocellular carcinoma(HCC)is one of the most common cancers worldwide and has a high ***,the treatment options for advanced HCC are limited to tyrosine kinase inhibitors,such as sorafenib and *** previous regimens have an insufficient efficacy,the combination therapy of atezolizumab and bevacizumab(Ate/Bev)has been investigated,which showed an improvement in progression-free and overall ***,the adverse events of this combination therapy in advanced HCC have not been ***,we report a novel case of an unresectable HCC and acute respiratory distress syndrome(ARDS)after a combination therapy of Ate/*** SUMMARY An 82-year-old male visited our outpatient clinic for an incidentally detected liver *** magnetic resonance imaging and enhanced chest computed tomography(CT)were performed,which showed arterial hyperenhancement with washout in delayed phase suggesting HCC,and a well-defined metastatic solid nodule,respectively.F-18 fluorodeoxyglucose positron emission tomography(PET)-CT exhibited multiple hypermetabolic lesions in the iliac bone,lumbar vertebrae,and *** of the high burden of the intrahepatic tumor,transarterial radioembolization was initially performed;after 37 d,a combination therapy of Ate/Bev was *** patient visited the emergency department three days after Ate/Bev treatment complaining of *** was diagnosed with severe pneumonitis based on *** administering oxygen via a high-flow nasal cannula,the P/F ratio was only 74;therefore,the patient was diagnosed with ARDS based on the overall examination *** tidal volume with high positive end-expiratory pressure,sedative agents combined with a neuromuscular blocker,and a systemic steroid were promptly applied to manage the ***,the patient did not recover from the hypoxia and expired 31 h after being *** Clinicians should be aware of severe pneumonitis due to the immune-

关键词： Hepatocellular carcinoma Systemic therapy Adverse events Pneumonitis Atezolizumab Acute respiratory distress syndrome

Time-dependent effect of combination therapy with erythropoietin and granulocyte colony-stimulating factor in a mouse model of hypoxic-ischemic brain injury

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Neuroscience Bulletin 2014年第1期30卷 107-117页

作者： Ji Hea Yu Jung Hwa Seo jong eun lee Ji Hoe Heo Sung-Rae Cho Department and Research Institute of Rehabilitation Medicine Yonsei University College of Medicine Brain Korea 21 PLUS Project for Medical Science Yonsei University Graduate Program of Nano Science and Technology Yonsei University Department of Anatomy Yonsei University College of Medicine Department of Neurology Yonsei University College of Medicine Yonsei Stem Cell Center Avison Biomedical Research CenterYonsei University College of Medicine Rehabilitation Institute of Neuromuscular Disease Yonsei University College of Medicine

Erythropoietin （EPO） and granulocyte colony- stimulating factor （G-CSF） are likely to play broad roles in the brain. We investigated the effects of combination therapy with EPO and G-CSF in hypoxic- ischemic brain injury during the acute, subacute, and chronic phases. A total of 79 C57BL/6 mice with hypoxic-ischemic brain injury were randomly assigned acute （days 1-5）, subacute （days 11-15）and chronic （days 28-32） groups. All of them were treated with G-CSF （250 μg/kg） and EPO （5 000 U/kg） or saline daily for 5 consecutive days. Behavioral assessments and immunohistochemistry for angiogenesis, neuro- genesis, and astrogliosis were performed with an 8-week follow-up. Hypoxia-inducible factor-1 （HIF-1） was also measured by Western blot analysis. The results showed that the combination therapy with EPO and G-CSF in the acute phase significantly improved rotarod performance and forelimb-use symmetry compared to the other groups, while subacute EPO and G-CSF therapy exhibited a modest improvement compared with the chronic saline controls. The acute treatment significantly increased the density of CD31^＋（PECAM-1） and a-smooth muscle actin^＋ vessels in the frontal cortex and striatum, increased BrdU^＋/PSA- NCAM^＋ neurogenesis in the subventricular zone, and decreased astroglial density in the striatum. Furthermore, acute treatment significantly increased the HIF-1 expression in the cytosol and nucleus, whereas chronic treatment did not change the HIF-1 expression, consistent with the behavioral outcomes. These results indicate that the induction of HIF-1 expression by combination therapy with EPO and G-CSF synergistically enhances not only behavioral function but also neurogenesis and angiogenesis while decreasing the astroglial response in a time- dependent manner.

关键词： erythropoietin granulocyte colony-stimulating factor hypoxia-inducible factor-1 hypoxic-ischemic brain injury

Defining and tracing subtypes of patient-derived xenograft models in pancreatic ductal adenocarcinoma

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Cancer Communications 2024年第8期44卷 915-920页

作者： Sangyeop Hyun Youngmin Han Jae Yun Moon Young-Ah Suh Won-Gun Yun Wooil Kwon jong-eun lee Daeun Kim Ja-Lok Ku Jin-Young Jang Daechan Park Department of Molecular Science and Technology Ajou UniversitySuwonRepublic of Korea Department of Surgery and Cancer Research Institute Seoul National University College of MedicineSeoulRepublic of Korea Molecular Science and Technology Research Center Ajou UniversitySuwonRepublic of Korea DNA Link Inc. SeoulRepublic of Korea Korean Cell Line Bank Laboratory of Cell BiologyCancer Research InstituteSeoul National University College of MedicineSeoulRepublic of Korea

Patient-derived xenograft(PDX)models have been used to explore therapeutic opportunities for pancreatic ductal adenocarcinoma(PDAC)[1].Although original tumor characteristics are altered by cancer-stromal interactions... 详细信息

关键词： adenocarcinoma ductal xenograft

Curcumin-induced cell death depends on the level of autophagic flux in A172 and U87MG human glioblastoma cells

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Chinese Journal of Natural Medicines 2020年第2期18卷 114-122页

作者： lee jong-eun Yoon Sung Sik lee Jae-Wook Moon eun-Yi Department of Bioscience and Biotechnology Sejong UniversitySeoul 05006Republic of Korea

Glioblastoma is the deadliest neoplasm with the worst 5-year survival rate among all human *** promotes autophagic cell death or blocks the induction of apoptosis in eukaryotic ***,we investigated whether varying levels of autophagic flux in glioblastoma lead to different efficacies of curcumin treatment using U87 MG and A172 human glioblastoma *** number of LC3 puncta,the number of cells with LC3 puncta and the level of LC3 Ⅱ,Atg5 and Atg7 protein were higher in U87 MG cells compared with A172 *** the cells were incubated with curcumin for 24 or 48 h,the percentage of cell death was higher in A172 cells compared with U87 MG *** the level of LC3 was lower,that of curcumin-induced LC3 was higher,in A172 cells than in U87 MG *** relative increases in cell death and LC3-mediated autophagy were greater under serum starvation in A172 cells compared with U87 MG ***-induced A172 cell death was reduced by serum *** both types of cells were transfected with LC3-GFP,the percentage of cell death was higher in A172 cells than that in U87 MG *** together,the data demonstrate that curcumin-mediated tumor cell death is regulated by the basal level of autophagic flux in different glioblastoma *** suggests that prior to the use of various curcumin therapeutics,the level of basal or induced autophagic flux should be carefully examined in tumor cells for the best efficacy.

关键词： Glioblastoma A172 U87MG Autophagic flux Curcumin

The use of artificial nest boxes by Siberian flying squirrels (Pteromys volans) in South Korea

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Journal of Forestry Research 2019年第3期30卷 1131-1137页

作者： jong-U Kim Woo-shin lee eun-Jae lee Department of Forest Sciences Seoul National University Seoul 08826Republic of Korea Urban Planning Research Division Daejeon Sejong Research InstituteDaejeon 34863Republic of Korea

In this study, we examined the use of artificial nest boxes by Siberian flying squirrels (Pteromys volans) in three coniferous and mixed forests in Gangwon Province, South Korea. Six hundred and twelve boxes with different sized entry holes (ranging from 3 to 7 cm in diameter) were placed in the forests between 2004 and 2009. Pteromys volans used nine boxes in the coniferous forests and two boxes in the mixed forests. The squirrels only used boxes with entrance holes measuring 3.5, 4, and 5 cm in diameter, showing a strong and moderate preference for boxes with 5 and 4-cm holes, respectively, and a strong avoidance for boxes with 3- and 7-cm holes. Therefore, we suggest placing artificial nest boxes with entrance holes 5 cm in diameter to encourage breeding activity. Most nests made in the artificial boxes were composed of fibrous materials from woody vines. We recommend placing artificial nest boxes with holes of 5-cm diameter in coniferous forests, which support dense populations of P. volans, to survey whether this approach would positively affect the breeding habits and population maintenance of this species.

关键词： Artificial nest box Coniferous forests Nest materials Siberian flying squirrel Woody vines

Clinical efficacy of 0.05%cyclosporine nano-emulsion in the treatment of dry eye syndrome associated with meibomian gland dysfunction

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International Journal of Ophthalmology(English edition) 2022年第12期15卷 1924-1931页

作者： Yeon Ji Jo Ji eun lee jong Soo lee Efficacy of nano-cyclosporine to DES with MGD 1 Department of Ophthalmology Pusan National University School of Medicine&Medical Research InstitutePusan National University HospitalPusan 49241Republic of Korea Department of Ophthalmology Pusan National University School of Medicine&Medical Research CenterYangsan Pusan National University HospitalYangsan 626-770Republic of Korea

AIM:To evaluate the clinical efficacy of topical 0.05%cyclosporine nano-emulsion in the treatment of dry eye syndrome(DES)with meibomian gland dysfunction(MGD).METHODS:This prospective study included 64 patients with DES and MGD who were randomly assigned to three groups:Group 1(n=24,conventional cyclosporine),Group 2(n=21,nano-emulsion cyclosporine),and Group 3(n=19,control).Lid margin telangiectasia(LMT),meibomian gland secretion(MGS),conjunctival injection(CI),corneal staining(CS),tear break-up time(TBUT),Schirmer test I(STI),Ocular Surface Disease Index(OSDI),and lipid layer thickness(LLT)was evaluated at 4,8,and 12wk of ***:In Group 3(control),LMT,CS,and CI improved after 8wk,MGS,TBUT after 12wk of *** Group 1(conventional cyclosporine),LMT,MGS,and TBUT improved significantly after 4wk,whereas CS,CI,STI,and LLT improved significantly after 8wk,and OSDI at *** Group 2(nano-cyclosporine),LMT,MGS,CS,CI,TBUT,and OSDI significantly improved after 4wk,and STI after ***,LLT was significantly higher than other groups after ***:Cyclosporine and nano-cyclosporine shows significant improvement in DES with MGD than the control *** contrast,the nano-cyclosporine group shows more statistically improved CI and CS at 4wk,especially LLT at 4,8,and 12wk compared to the conventional cyclosporine group.

关键词： anti-inflammatory treatment cyclosporine dry eye disease meibomian gland dysfunction

Comparison of cytotoxicity and wound healing effect of carboxymethylcellulose and hyaluronic acid on human corneal epithelial cells

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International Journal of Ophthalmology(English edition) 2015年第2期8卷 215-221页

作者： jong Soo lee Seung Uk lee Cheng-Ye Che Ji-eun lee Department of Ophthalmology School of MedicinePusan National University Biomedical Research Institute Pusan National University Hospital Department of Ophthalmology School of Medicine Kosin University Department of Ophthalmology the Affiliated Hospital of Medical College Qingdao University Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan Hospital

AIM: To investigate the cytotoxic effect on human corneal epithelial cells(HCECs) and the ability to faciliate corneal epithelial wound healing of carboxymethylcellulose(CMC) and hyaluronic acid(HA).METHODS: HCECs were exposed to 0.5% CMC(Refresh plus, Allergan, Irvine, California, USA) and 0.1% and 0.3%HA(Kynex , Alcon, Seoul, Korea, and Hyalein mini,Santen, Osaka, Japan) for the period of 30 min, and 4, 12,and 24 h. Methyl thiazolyl tetrazoiun(MTT)-based calorimetric assay was performed to assess the metabolic activity of cellular proliferation and lactate dehydrogenase(LDH) leakage assay to assess the cytotoxicity. apoptotic response was evaluated with flow cytometric analysis and fluorescence staining with Annexin V and propiodium iodide. Cellular morphology was evaluated by inverted phase-contrast light microscopy and electron microscopy. The wound widths were measured 24 h after confluent HCECs were scratch ***: The inhibitory effect of human corneal epithelial proliferation and cytotoxicity showed the time-dependent response but no significant effect. Apoptosis developed in flow cytometry and apoptotic cells weredemonstrated in fluorescent micrograph. The damaged HCECs were detached from the bottom of the dish and showed the well-developed vacuole formations. Both CMC and HA stimulated reepithehlialization of HCECs scratched, which were more observed in ***: CMC and HA, used in artificial tear formulation, could be utilized without any significant toxic effect on HCECs. Both significantly stimulated HCEC reepithelialization of corneal wounds.

关键词： carboxymethylcellulose corneal wound healing human corneal epithelial cells hyaluronic acid cytotoxicity

Efficient photovoltaic effect in graphene/h-BN/silicon heterostructure self-powered photodetector

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Nano Research 2021年第6期14卷 1967-1972页

作者： Ui Yeon Won Boo Heung lee Young Rae Kim Won Tae Kang Ilmin lee Ji eun Kim Young Hee lee Woo jong Yu Department of Electrical and Computer Engineering Sungkyunkwan UniversitySuwon16419Republic of Korea Center for Integrated Nanostructure Physics Institute for Basic Science(IBS)Suwon16419Republic of Korea

Graphene(Gr)/Si-based optoelectronic devices have attracted a lot of academic attention due to the simpler fabrication processes,low costs,and higher performance of their two-dimensional(2D)/three-dimensional(3D)hybrid interfaces in Schottky junction that promotes electron-hole ***,due to the built-in potential of Gr/Si as a photodetector,the Iph/Idark ratio is often hindered near zero-bias at relatively low illumination *** is a major drawback in self-powered *** this study,we have demonstrated a self-powered van der Waals heterostructure photodetector in the visible range using a Gr/hexagonal boron nitride(h-BN)/Si structure and clarified that the thin h-BN insertion can engineer asymmetric carrier transport and avoid interlayer coupling at the *** dark current was able to be suppressed by inserting an h-BN insulator layer,while maintaining the photocurrent with minimal decrease at near *** a result,the normalized photocurrent-to-dark ratio(NPDR)is improved more than 104 ***,both Iph/Idark ratio and detectivity,increase by more than 104 times at−0.03 V drain *** proposed Gr/h-BN/Si heterostructure is able to contribute to the introduction of next-generation photodetectors and photovoltaic devices based on graphene or silicon.

关键词： graphene hexagonal boron nitride van der Waals heterostructure self-powered Research