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Development and validation of an immunogenic cell death-related prognostic model, and landscape of the tumor immune microenvironment in glioblastoma

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Genes & Diseases 2024年第3期11卷 72-74页

作者： Sha Yang Mei Deng Yunbiao Xiong Bing Xiong Junwu Fu Jia Wang jiqin zhang Guoqiang Han Jian Liu Ying Tan Guizhou University Medical College GuiyangGuizhou 550025China Department of Neurosurgery Guizhou Provincial People's HospitalGuiyangGuizhou 550002China Department of Anesthesiology Guizhou Provincial People's HospitalGuiyangGuizhou 550002China Department of Neurosurgery Chongqing Emergency Medical CenterChongqing400000China

The important role of immunogenic cell death(ICD)in many tumors is increasingly being ***,its mechanisms and potential as a biomarker and therapeutic target in glioblastoma(GBM)have not been well *** obtained GBM samples from the Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases,as well as the immunotherapy cohort from the IMvigor210 *** used unsupervised clustering to obtain two ICDrelated clusters,corresponding to the ICD-low and ICD-high subtypes respectively,and the tumor immune microenvironment and prognosis of the two subtypes were significantly different.

关键词： immune Cancer prognosis

Harnessing the power of gene-editing to develop the next generation of CAR-T cells

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National Science Open 2023年第5期2卷 5-10页

作者： Bing Du jiqin zhang Mingyao Liu Shanghai Frontiers Science Center of Genome Editing and Cell Therapy Shanghai Key Laboratory of Regulatory BiologyInstitute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghai 200241China

The manufacturing workflow of CAR-T cells makes it easy to be further modified by gene editing *** rapid development of gene editing technology not only unveiled the mystery of T cells,but also provided a huge space for the optimization and development of the CAR-T ***,we review the crucial role of gene editing in improving the safety,efficacy and accessibility of CAR-T cells in clinic.

关键词： editing CAR power

Prehistoric damaging earthquake promoted the decline of"Heluo Ancient State"in Early China

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Science China Earth Sciences 2023年第5期66卷 1120-1132页

作者： Xiu HU Peng LU Youli LI Duowen MO Wanfa GU Qingri LIU Ailun GUO Panpan CHEN Hui WANG Yan TIAN Xu WANG Yayi HU jiqin zhang Zhen WANG Quanxing LUO Junjie XU School of Urban and Environmental Science Peking UniversityBeijing100871China Institute of Geography Henan Academy of SciencesZhengzhou450052China Zhengzhou Institute of Cultural Relics and Archaeology Zhengzhou450000China The Institute of Archaeology Chinese Academy of Social SciencesBeijing100710China College of History Zhengzhou UniversityZhengzhou450000China School of Earth Sciences and Engineering Sun Yat-Sen UniversityGuangzhou510275China

The emergence of the"Ancient State"is considered an important stage in the discourse on human *** on its complex and hierarchical social structure,the Shuanghuaishu site of Henan Province is regarded as an"Ancient state",specifically the"Heluo Ancient State".The site is situated in the middle reaches of the Yellow River region and played a key role in prehistoric ***,the reasons behind its decline are ***,we conducted an integrated investigation of the archaeological excavations and archaeoseismic analyses at the Shuanghuaishu *** identified paleoseismic remains within paleo-artificial moats,which were formed owing to off-fault structures of soil liquefaction,hillslope landslides,and related ground fractures,along with shake-related wall disruption and building collapse in the residential *** dating was conducted to constrain the age for this earthquake event,which was determined to be approximately 5.0 cal kyr ***,based on the practical curve for earthquake-induced liquefaction,the preliminary estimate of the magnitude of this paleo seismic event is greater than M_(s)*** propose that the Shuanghuaishu site experienced a recession after the earthquake event,which may have influenced the continuous development of the"Heluo Ancient State"as a regional center settlement.

关键词： Shuanghuaishu site Heluo Ancient State Yangshao culture Archaeoseismology Geohazards

Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants

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Protein & Cell 2018年第9期9卷 814-819页

作者： Lei Yang Xiaohui zhang Liren Wang Shuming Yin Biyun Zhu Ling Xie Qiuhui Duan Huiqiong Hu Rui Zheng Yu Wei Liangyue Peng Honghui Han jiqin zhang Wenjuan Qiu Hongquan Geng Stefan Siwko Xueli zhang Mingyao Liu Dali Li East China Normal University and Shanghai Fengxian DistrictCentral Hospital Joint Center for Translational Medicine ShanghaiKey Laboratory of Regulatory Biology School of Life SciencesEast China Normal University Shanghai 200241 China Fengxian Hospital Affiliated to Southern Medical University Shanghai 201499 China Xinhua Hospital Shanghai Jiao Tong University School ofMedicine Shanghai 200092 China School of Life Sciences Hunan Normal University Changsha 410081 China Bioray Laboratories Inc. Shanghai 200241 China Department of Molecular and Cellular Medicine The Institute of Biosciences and Technology Texas AM University Health Science Center Houston TX 77030 USA

Dear Editor, The clustered regularly interspaced short palindromic repeat （CRISPR） system has been widely adapted to genome editing to either introduce or correct genetic mutations （Wang et al., 2016）. However, due to competition with the dominant non-homologous end-joining （NHEJ） pathway, precise genome modifications through Cas9-stimulated homologous recombination （HR） is inefficient. Through fusion of cytidine deaminases, APOBEC1 （apolipoprotein B editing complex 1） or AID （activation-induced deaminase）, with Cas9 variants, several groups have developed the cytidine base editor （BE） systems （Komor et al., 2016; Li et al., 2018; Nishida et al., 2016）. The BE system achieves programmable conversion of C-G base pairs to T.A without double-stranded DNA cleavage （Zhou et al., 2017）. More recently, adenine base editors （ABEs）, which efficiently convert A-T base pairs to G-C in genomic DNA, have been developed via fusion of an engineered tRNA adenosine deaminase （ecTadA from Escherichia coh） with nCas9 （Gaudelli et al., 2017）. The ABE system has quickly been adapted to generate disease models and correction of genetic disease in mice （Ryu et al., 2017; Liu et al, 2018）. However, whether the editing efficiency and the targeting scope of ABE could be improved is largely unexplored. In this study, we describe the efficient generation of base-edi- ted mice and rats modeling human diseases through ABEs with highest efficiency up to 100%. We also demonstrate an increase of ABE activity through injection of chemically modified tracrRNA and crRNA in mouse zygotes, and the expansion of editing scope by fusion of an ecTadA mutant to SaCas9n-KKH and Casgn-VQR variants in both cells and embryos. Our study suggests that the ABE system is a powerful and convenient tool to introduce precise base conversions in rodents.

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变量施肥滞后时间检测与位置修正方法研究

农业机械学报 2021年第S1期52卷 74-80页

作者：刘刚胡号黄家运张季琴中国农业大学现代精细农业系统集成研究教育部重点实验室北京100083 中国农业大学农业农村部农业信息获取技术重点实验室北京100083

针对变量施肥位置滞后问题,在已有研究的基础上,以提高变量施肥机作业精度为目标,利用压力传感器、ArduinoUNO等设备搭建了滞后时间检测系统,并建立了落肥时间及其影响因素之间的关系模型,以该模型为数据基础提出了基于滞后距离的施肥... 详细信息

针对变量施肥位置滞后问题,在已有研究的基础上,以提高变量施肥机作业精度为目标,利用压力传感器、ArduinoUNO等设备搭建了滞后时间检测系统,并建立了落肥时间及其影响因素之间的关系模型,以该模型为数据基础提出了基于滞后距离的施肥位置修正方法。该方法利用滞后时间和作业速度计算滞后距离,当机具与网格边界距离与滞后距离相等时改变施肥状态,实现施肥位置的修正。田间试验结果表明:本文提出的滞后时间检测方法的检测精度为84.1%;建立的转速-滞后时间模型能够准确计算滞后时间,基于滞后距离的施肥位置修正方法能够有效提高施肥位置精度,施肥位置滞后距离减小了61.8%。

关键词：变量施肥滞后时间位置滞后滞后修正

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排肥单体独立控制的双变量施肥控制系统研制

农业工程学报 2021年第10期37卷 38-45页

作者：张季琴刘刚胡号黄家运中国农业大学现代精细农业系统集成研究教育部重点实验室北京100083 宁夏大学机械工程学院银川750021 中国农业大学农业农村部农业信息获取技术重点实验室北京100083

针对当前变量施肥机无法根据实际田块尺寸调整作业行数,进而调节作业幅宽的问题,该研究通过改造玉米播种施肥机的排肥驱动方式,设计了一种排肥单体独立控制的双变量施肥控制系统。首先通过二次多项式拟合方法,构建了排肥单体的双变量控... 详细信息

针对当前变量施肥机无法根据实际田块尺寸调整作业行数,进而调节作业幅宽的问题,该研究通过改造玉米播种施肥机的排肥驱动方式,设计了一种排肥单体独立控制的双变量施肥控制系统。首先通过二次多项式拟合方法,构建了排肥单体的双变量控制模型;然后对排肥单体的定位方法进行了分析,提出排肥单体独立控制系统;最后对各行排肥量一致性、不同车速下的排肥量控制准确性以及各行独立控制性能进行试验研究。结果表明,在排肥轴转速为10~60 r/min的区间内,各行平均排肥量一致性变异系数为3.35%;在目标排肥量为350 kg/hm2,作业车速为7 km/h的条件下,排肥量控制精度达到97.6%;对于凸、凹和S形3种不同形状的施肥边界,各行排肥滞后距离相对于作业幅宽的变化率均小于15%。系统具有较高的控制准确性和稳定性,能够适应复杂施肥边界,可为玉米基肥变量施用装备的创新性研发提供技术参考。

关键词：农业机械玉米基肥双变量排肥单体独立控制

Watermarking Technique Based On Wavelet Transform For Color Images

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Watermarking Technique Based On Wavelet Transform For Color ...

油菜机械直播铲锹式种床整备机振动特性分析与结构改进

第24届中国控制与决策会议

作者： Ying zhang, jiqin Wang, Xuebo Chen University of Science and Technology LiaoNing, Anshan 114051, China

Watermarking techniques have been proposed as an effective method to protecting the copyright of multimedia data. At present, a large proportion of the watermarking algorithms embed the grey image into the carrier image. In this paper, a watermarking algorithm for color images based on wavelet analysis is proposed. The algorithm scrambled the original watermark image in pretreatment, and used the wavelet transform to process the carrier image and the scrambled watermark image. Then the color watermarked image was embedded into the low-frequency discrete wavelet coefficient of the color carrier image. The experimental results demonstrate that the algorithm can assure the security of the watermark image, and the watermark image has good invisibility and strong robustness for common signal processing and attack.

关键词： Digital Watermark wavelet transform robustness

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农业工程学报 2023年第19期39卷 39-48页

作者：林建新廖庆喜张青松康艳张季钦华中农业大学工学院武汉430070 农业农村部长江中下游农业装备重点实验室武汉430070

针对油菜机械直播铲锹式种床整备机作业时机组振动大,易导致关键零部件磨损、降低整机可靠性的问题,该研究利用Coco-80动态信号测试分析系统对机组在怠速和田间作业工况下的振动加速度进行测试,采用时域和频域分析相结合的方法,确定了... 详细信息

针对油菜机械直播铲锹式种床整备机作业时机组振动大,易导致关键零部件磨损、降低整机可靠性的问题,该研究利用Coco-80动态信号测试分析系统对机组在怠速和田间作业工况下的振动加速度进行测试,采用时域和频域分析相结合的方法,确定了两种工况下不同位置测点的振动加速度大小及机组主要激振源,并以此为依据提出了通过优化曲柄相位角排列方式降低整机所受合惯性力、合惯性力矩,减小铲锹式种床整备机振动的方案。进一步地,基于质量代换法建立了整机共10组曲柄摇杆机构的惯性力、惯性力矩受力模型,运用MATLAB软件分析得出整机受到合惯性力、合惯性力矩最小的曲柄相位角排列方式,在此排列方式下,整机合惯性力基本平衡,绕x、y轴的合惯性力矩绝对值均值分别降低了37.40%、30.09%。对改进后的机组在振动较大处采集加速度时域信号,与改进前相比,测点振动总量在怠速和田间作业工况下分别降低了36.42%、31.97%。该研究可为基于平面连杆机构群的农业机械减振研究提供参考。

关键词：振动油菜惯性力铲锹式种床整备机曲柄摇杆机构

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加密经济学研究进展

经济学动态 2023年第11期 125-140页

作者：冯碧梅王诗桪张继钦福州大学经济与管理学院 350116

加密经济学作为一个新兴交叉研究领域,综合运用了经济学、密码学、计算机科学等多学科理论,主要研究如何在分布式系统中形成共识。本文在总结梳理加密经济学的学科属性、理论渊源的基础上,对加密经济学的研究对象、发展演进、应用场景... 详细信息

加密经济学作为一个新兴交叉研究领域,综合运用了经济学、密码学、计算机科学等多学科理论,主要研究如何在分布式系统中形成共识。本文在总结梳理加密经济学的学科属性、理论渊源的基础上,对加密经济学的研究对象、发展演进、应用场景等进行了梳理。首先,本文通过回顾“拜占庭将军问题”介绍了加密经济学的起源,并阐述了加密经济学的研究对象、理论基础和学科属性。然后,本文梳理了加密经济学在共识算法、激励机制、智能合约等方面的发展演进。最后,本文总结了加密经济学在分布式系统的去中心化特征、安全性特征、可拓展性特征等方面的应用研究,并对加密经济学的未来研究进行了展望。

关键词：加密经济学共识机制工作量证明权益证明