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中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会

作者： Wei Guo Sudhanshu Mishra jingsi tang Bo Zeng Ruihong Ning Ying Li Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University

The giant panda(Ailuropoda melanoleuca) is a highly vulnerable mammal(The IUCN Red List of Threatened Species,2016),whilst being the most iconic and flagship species for wildlife conservation *** giant panda,a member of family Ursidae and possessing a typical carnivore like short gastrointestinal tract,is an enigmatic species because it consumes nearly 12.5 kg of highly fibrous bamboo leaves and stems each day,and it became full-time bamboo eating species by the late Pliocene(2 Mya).Along with the threat of climate change,this bamboo specialist is at risk to extinction because of its sole reliance on large quantities of low-nutrition and low-energy content food from *** profound investigation of Panda’s genome has reported that it encodes all enzymes crucial for a carnivorous digestive system but lacks those required for digesting cellulose rich bamboo *** the excellent studies on Tas1r1pseudogenization(umami taste receptor gene) and appetite-reward system in the panda’s genome to understand the evolutionary consequences of dietary switch of the panda,there is still uncertainty about the underlying mechanisms and one question still remains unanswered;"why pandas shifted to bamboo diet?" Under such circumstances,it was believed that the giant panda is dependent on gut microbiota in order to adapt to highly fibrous bamboo ***,gastrointestinal disease is reported to be a primary cause of death in both wild and ***,it becomes most significant to understand,how gut microbiota impacts panda’s health and nutrition,and biology behind this *** metagenomic study found the Clostridium bacteria in giant panda gut contains genes known to code enzymes that digest cellulose(Zhu et al.,2011),which was opposed later by a current study with larger sample size of captive pandas which suggested that panda’s carnivore-like gut microbiota has not evolved to efficiently digest cellulose from a fibre-rich bamboo diet(Xue et al.201

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助产一对一全程陪伴在高龄产妇分娩中的应用效果观察

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中国医药导报 2012年第24期9卷 136-137页

作者：唐静思重庆市大足区人民医院产科重庆402360

目的探讨高龄产妇实施助产一对一全程陪伴的效果。方法选择2009年6月～2010年6月在我院住院分娩的高龄产妇120例,按随机数字表法将其分为观察组和对照组,每组各60例。对照组按传统助产模式分娩,观察组实施助产一对一全程陪伴。比较两组... 详细信息

目的探讨高龄产妇实施助产一对一全程陪伴的效果。方法选择2009年6月～2010年6月在我院住院分娩的高龄产妇120例,按随机数字表法将其分为观察组和对照组,每组各60例。对照组按传统助产模式分娩,观察组实施助产一对一全程陪伴。比较两组心理状态、分娩方式、产后出血和新生儿情况。结果观察组产妇的SAS评分、焦虑发生率及产后出血发生率和产后出血量均显著低于对照组(P<0.01),自然分娩例数明显多于对照组(P<0.01),而剖宫产例数明显少于对照组(P<0.01),自然分娩产妇的第一产程、第二产程及总产程时间均显著短于对照组(P<0.05),新生儿正常率明显高于对照组(P<0.01)。结论助产一对一全程陪伴能有效减轻高龄产妇焦虑情绪,提高自然分娩率,缩短产程,减少产后出血和新生儿窒息,值得在高龄产妇中推广应用。

关键词：助产一对一全程陪伴高龄产妇分娩

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蓝靛果花青素对乳酸菌生长及酸奶后酸化的影响

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中国酿造 2020年第11期39卷 147-152页

作者：那治国高悦露唐敬思胡维付红岩黑龙江东方学院食品与环境工程学部黑龙江哈尔滨150066

采用野生蓝靛果(Lonicera caerulea)纯化得到花青素,以生长曲线、抑菌圈直径、最小抑菌浓度为评价指标,研究其对嗜热链球菌(Streptococcus thermophilus)、保加利亚乳杆菌(Lactobacillus bulgaricus)生长的影响,分析其抑菌效果、抑菌机... 详细信息

采用野生蓝靛果(Lonicera caerulea)纯化得到花青素,以生长曲线、抑菌圈直径、最小抑菌浓度为评价指标,研究其对嗜热链球菌(Streptococcus thermophilus)、保加利亚乳杆菌(Lactobacillus bulgaricus)生长的影响,分析其抑菌效果、抑菌机理及保质期21 d内酸度、pH值、还原糖的变化。结果表明,添加花青素组能抑制菌株生长;花青素对两种乳酸菌及其混合菌有一定的抑制作用,对嗜热链球菌、混合菌(1∶1)及保加利亚乳杆菌的最小抑菌浓度分别为62.5μg/mL、62.5μg/mL、31.25μg/mL;加热预处理、培养基pH以及糖浓度对花青素的抑菌作用产生影响。花青素对乳酸菌的抑菌作用机理是其改变细胞膜通透性、损伤菌体蛋白引起菌体蛋白的泄露。0.32%花青素添加量可抑制酸奶后酸化作用。

关键词：蓝靛果花青素乳酸菌后酸化

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利用β沸石废液为部分营养物质合成低硅/铝比多级孔ZSM-48分子筛

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石油学报（石油加工） 2017年第2期33卷 227-233页

作者：唐静思高禾鑫孔庆岚于峰李鹏孙晓勃郑家军李瑞丰太原理工大学能源化工与催化研究中心山西太原030024

以合成β沸石的废液作为部分原料合成了低硅多级ZSM-48,利用XRD、XRF、SEM、N_2吸附-脱附、NH3-TPD、FT-IR等手段详细表征了合成样品的结构性能。结果表明,β沸石废弃液中的硅铝物种以初级(次级)结构单元形式存在,这些初级(次级)结构单... 详细信息

以合成β沸石的废液作为部分原料合成了低硅多级ZSM-48,利用XRD、XRF、SEM、N_2吸附-脱附、NH3-TPD、FT-IR等手段详细表征了合成样品的结构性能。结果表明,β沸石废弃液中的硅铝物种以初级(次级)结构单元形式存在,这些初级(次级)结构单元诱导了ZSM-48沸石晶核的形成,并促进了晶体生长;合成的ZSM-48为棒状纳米初级晶粒构成的多晶聚集体,且存在丰富的介孔结构;可以在较大范围内调节合成的ZSM-48的n(SiO2)/n(Al2O3),获得n(SiO_2)/n(Al_2O_3)为52~76的高结晶的低硅ZSM-48样品。此方法为合成低硅ZSM-48提供了一个绿色、环境友善、低成本的合成途径。

关键词：富铝ZSM-48 废液多级环境友善低成本

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低能N^+注入诱变选育弱后酸化保加利亚乳杆菌

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中国酿造 2019年第12期38卷 112-115页

作者：那治国唐敬思王红梅钱镭轩诗涛黑龙江东方学院食品与环境工程学部黑龙江哈尔滨150066 黑龙江东方学院总务处黑龙江哈尔滨150066

为获得弱后酸化保加利亚乳杆菌(Lactobacillus bulgaricus),采用低能N+注入技术对保加利亚乳杆菌DL1进行诱变,经中间培养后用青霉素进行筛选。结果表明,在注入能量为25 keV、注入剂量为1.5×1015 ions/cm2的条件下进行诱变,并用2.0 mg/m... 详细信息

为获得弱后酸化保加利亚乳杆菌(Lactobacillus bulgaricus),采用低能N+注入技术对保加利亚乳杆菌DL1进行诱变,经中间培养后用青霉素进行筛选。结果表明,在注入能量为25 keV、注入剂量为1.5×1015 ions/cm2的条件下进行诱变,并用2.0 mg/mL青霉素处理2 h后,通过筛选最终得到一株后酸化较弱的突变菌株DL1-3。与出发菌株DL1相比,突变菌株DL1-3在25℃条件下贮藏后,后酸化程度降低18.8%,且在42℃条件下发酵脱脂乳的产酸能力差异较小,并可稳定遗传。

关键词：后酸化保加利亚乳杆菌低能N+注入诱变选育

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Captive breeding alters the gut microbiome of giant panda

Captive breeding alters the gut microbiome of giant panda

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中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Wei Guo jingsi tang Sudhanshu Mishra Chengdong Wang Hemin Zhang Jiangchao Zhao Fanli Kong Bo Zeng Ruihong Ning JiANDong Yang Mingyao Yang Mingwang Zhang Qingyong Ni Yan Li Caiwu Li Desheng li Yingping Xiao Hua Yang Ying Li Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University Department of Laboratory Medicine Chengdu Medical College China Conservation and Research Center for the Giant Panda Department of Animal Science Division of AgricultureUniversity of Arkansas Institute of Quality and Standard for Agro-products Zhejiang Academy of Agricultural Sciences

Captive breeding has been used as an effective approach to protect endangered animals but its effect on the gut microbiome and the conservation status of these species is largely *** giant panda is a symbol of *** integrated efforts including captive breeding,this species has been recently upgraded from "endangered" to "vulnerable".Since a large proportion(21.8%) of the population is captive,it is critical to understand how captivity changes the gut microbiome of these pandas and how such alterations to the microbiome might affect their future fitness and potential impact on the ecosystem after release into the *** we use 16 S rRNA marker gene sequencing and shotgun metagenomics sequencing to demonstrate that the fecal microbiomes differ substantially in wild and captive giant *** microbiome diversity was significantly lower in captive pandas,as was the diversity of functional ***,captive pandas had reduced functional potential for cellulose degradation but enriched metabolic pathways for starch metabolism,indicating a reduced fitness when releasing into the wild since their major diet in the wild will be ***,antibiotic resistance genes and virulence factors,as well as heavy metal tolerance genes were enriched in the microbiomes of captive pandas,which raises a huge concern of spreading these genes to other wild animals and the ecosystem after releasing them to the wild *** results clearly show that captivity has altered the giant panda microbiome,which could have unintended negative consequences on their fitness and the ecosystem during the re-introduction of giant pandas into the wild.

关键词： captivity gut microbiome giant pandas antibiotic resistance genes metagenomics diversity metal tolerance genes virulence factors

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High-altitude living shapes the skin microbiome in humans and pigs

High-altitude living shapes the skin microbiome in humans an...

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中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Bo Zeng Jiangchao Zhao Wei Guo Siyuan Zhang Yutong Hua jingsi tang Fanli Kong Xuewu Yang Lizhi Fu Kun Liao Xianqiong Yu Guohong Chen Long Jin Surong Shuai Jiandong Yang Xiaohui Si Ruihong Ning Sudhanshu Mishra Ying Li Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University Division of Agriculture Department of Animal ScienceUniversity of Arkansas

While the skin microbiome has been shown to play important roles in health and disease in several species,the effects of altitude on the skin microbiome and how high-altitude skin microbiomes may be associated with health and disease states remains largely *** 16 S rRNA marker gene sequencing,we characterized the skin microbiomes of people from two racial groups(the Tibetans and the Hans) and of three local pig breeds(Tibetan pig,Rongchang pig,and Qingyu pig) at high and low *** skin microbial communities of low-altitude pigs and humans were distinct from those of high-altitude pigs and humans,with five bacterial taxa(Arthrobacter,Paenibacillus,Carnobacterium,and two unclassified genera in families Cellulomonadaceae and Xanthomonadaceae) consistently enriched in both pigs and humans at high *** diversity was also significantly lower in skin samples collected from individuals living at high altitude compared to individuals at low *** of the taxa unique to high-altitude humans and pigs are known extremophiles adapted to harsh environments such as those found at high *** our data reveal that altitude has a significant effect on the skin microbiome of pigs and humans.

关键词： Skin microbiome high altitude Tibetans Tibetan pigs 16S rRNA

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特种计算机设备增强属性研究

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电子测试 2023年第4期 37-41页

作者：刘俊时刚陈健华唐静思中国电子科技集团公司第三十二研究所上海201808

基于特种计算机的要求,开展增强属性的研究。增强属性的内涵是一系列加固特性的集合,体现了特种计算机设备超越商用计算机的能力。其不仅具备抗恶劣环境(振动、冲击、高低温、潮湿、沙尘、盐雾、霉菌、强电磁脉冲等)的能力,而且具备可... 详细信息

基于特种计算机的要求,开展增强属性的研究。增强属性的内涵是一系列加固特性的集合,体现了特种计算机设备超越商用计算机的能力。其不仅具备抗恶劣环境(振动、冲击、高低温、潮湿、沙尘、盐雾、霉菌、强电磁脉冲等)的能力,而且具备可靠性、安全性、实时性等技术方面的能力。特点主要体现在特种计算机应用广泛,不同的应用会对抗恶劣环境计算机的加固特性提出不同的要求,由此特种计算机的增强属性呈现出类别众多、专业性强、多学科协同等特点。特种计算机的增强属性按功能和性能属性可以分为抗恶劣环境相关的环境能力增强和可靠性相关的基础能力增强两类。

关键词：特种计算机环境能力增强基础能力增强可靠性安全性

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Metagenomic Study Suggests That the Gut Microbiota of the Giant Panda(Ailuropoda melanoleuca) May Not Be Specialized for Fiber Fermentation

Metagenomic Study Suggests That the Gut Microbiota of the Gi...

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中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Wei Guo Sudhanshu Mishra Jiangchao Zhao jingsi tang Bo Zeng Fanli Kong Ruihong Ning Miao Li Hengzhi Zhang Yutian Zeng Yuanliangzi Tian Yihang Zhong Hongdi Luo Yunhan Liu Jiandong Yang Mingyao Yang Mingwang Zhang Yan Li Qingyong Ni Caiwu Li Chengdong Wang Desheng Li Hemin Zhang Zhili Zuo Ying Li Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University Division of Agriculture Department of Animal ScienceUniversity of Arkansas Animal Nutrition Institute Sichuan Agricultural University College of Animal Science and Technology Sichuan Agricultural University China Conservation and Research Center for the Giant Panda Chengdu Zoo Chengdu Institute of Wildlife

Bamboo-eating giant panda(Ailuropoda melanoleuca) is an enigmatic species,which possesses a carnivore-like short and simple gastrointestinal tract(GIT).Despite the remarkable studies on giant panda,its diet adaptability status continues to be a matter of *** resolve this puzzle,we investigated the functional potential of the giant panda gut microbiome using shotgun metagenomic sequencing of fecal *** also compared our data with similar data from other animal species representing herbivores,carnivores,and omnivores from current and earlier *** found that the giant panda hosts a bear-like gut microbiota distinct from those of herbivores indicated by the metabolic potential of the microbiome in the gut of giant pandas and other ***,the relative abundance of genes involved in cellulose-and hemicellulose-digestion,and enrichment of enzymes associated with pathways of amino acid degradation and biosynthetic reactions in giant pandas echoed a carnivore-like *** significantly,the enzyme assay of the giant panda’s feces indicated the lowest cellulase and xylanase activity among major herbivores,shown by an in-vitro experimental assay of enzyme activity for cellulose and *** of our results consistently indicate that the giant panda is not specialized to digest cellulose and hemicellulose from its bamboo diet,making the giant panda a good mammalian model to’ study the unusual link between the gut microbiome and *** increased food intake of the giant pandas might be a strategy to compensate for the gut microbiome functions,highlighting a strong need of conservation of the native bamboo forest both in high-and low-altitude ranges to meet the great demand of bamboo diet of giant pandas.

关键词： Giant panda Gut microbiota metagenomics enzyme activity diet adaptability

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Sin1-mediated mTOR signaling in cell growth, metabolism and immune response

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National Science Review 2019年第6期6卷 1149-1162页

作者： Chun Ruan Xinxing Ouyang Hongzhi Liu Song Li jingsi Jin Weiyi tang Yu Xia Bing Su Shanghai Institute of Immunology Department of Immunology and Microbiology and the Minister of Education Key Laboratory of Cell Death and DifferentiationShanghai Jiao Tong University School of Medicine Zhiyuan College Shanghai Jiao Tong University

The mammalian target of rapamycin(mTOR) is an evolutionarily conserved Ser/Thr protein kinase with essential cellular function via processing various extracellular and intracellular inputs. Two distinct multi-protein m TOR complexes(mTORC), m TORC1 and m TORC2, have been identified and well characterized in eukaryotic cells from yeast to human. Sin1, which stands for ■,also known as mitogen-activated protein kinase■ (MAPKAP)1, is an evolutionarily conserved adaptor protein. Mammalian Sin1 interacts with many cellular proteins, but it has been widely studied as an essential component of m TORC2, and it is crucial not only for the assembly of m TORC2 but also for the regulation of its substrate specificity. In this review, we summarize our current knowledge of the structure and functions of Sin1, focusing specifically on its protein interaction network and its roles in the m TOR pathway that could account for various cellular functions of m TOR in growth,metabolism, immunity and cancer.

关键词： Sin1 AGC kinases mTOR complex Akt metabolism and immune response

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