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Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter

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Chinese Science Bulletin 1999年第8期44卷 698-701页

作者： Guocun Huang Yuemei Dong jingsan sun Chinese Acad Sci Inst Bot Beijing 100093 Peoples R China

The green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling has been verified according to its GFP-related fluorescence and Southern blotting analysis. The results provided direct and convincing facts in cytology and molecular biology for the pollen-tube pathway method, an efficient transformation technique used in plants.

关键词： green fluorescent protein (GFP) pollen-tube pathway cotton.

A polypoid apomictic germplasm was identified in Oryza sativa

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Chinese Rice Research Newsletter 1995年第4期3卷 11-12页

作者： LIU Yongsheng and sun jingsan,Inst of Botany,Acad Sinica ,Beijing 100044,China

Since apomixis has a close genetic linkage with polyploid, it seems impossible to find apomictic germplasm in rice, a typical diploid species. For these reasons, we have dedicated a large portion of our research effort to induce polyploid in rice and screening apomietic germplasm. As a result, a triploid line was identified cytoembryologically to possess diplospory which could be used to fix

关键词： line

Reproductive versatility of a polyembryonic line SB-1 of Oryza sativa L

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Rice Science 1995年第1期 3-4页

作者： LIU Yongsheng sun jingsan WANG Fuxiong Inst of Botany,Academia Sinica,Beijing 100044,China ZHOU Kaida Sichuan Agri Univ,Ya'an 625014,China

Cytoemhryological studies were employed to revealthe origin of haploid and to determine the cytologicalfacters relating to the production of diploid polyem-bryony within one embryo sac occuring in naturalpopulation of a polyembryonic line SB-1 of ***-va L.. The results showed that one or both syn-ergids may develop into embryos prior to fertilization

关键词： line Reproductive versatility of a polyembryonic line SB-1 of Oryza sativa L

Cloning and sequence analysis of a gene encoding polygalacturonase-inhibiting protein from cotton

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Progress in Natural Science:Materials International 2003年第2期 41-46页

作者： DOU Daolong, WANG Bingshan, TANG Yixiong, WANG Zhixing and sun jingsan ( Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China) Institute of Botany，Chinese Academy of Sciences，Beijing 100093 China Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China

Abstract Polygalacturonase- inhibiting proteins (PGIP) play important roles in plant defense of pathogen, especially fungi. A pairof degenerated primers is designed based on the conserved sequence of 20 other known pgip genes and used to amplify Gossypium barbadense cultivation 7124 cDNA library by touch-down PCR. A 561 bp internal fragment of the pgip gene is obtained and used to designthe primers for rapid amplification of cDNA ends. A composite pgip gene sequence is constructed from the products of 5' and 3' RACE,which are 666 bp and 906 bp respectively. Analysis of nucleic acid sequence shows 69.2% and 68. 7% similarity to Citrus and Ponciruspgip genes, respectively. Its open reading frame of the gene encodes a polypeptide of 330 amino acids, in which 10 leucine-rich repeats ar-range tandemly. A new set of primers is designed to the 5' and 3' ends of the gene, which allows amplification of the full-length gene fromthe cotton cDNA library. Genomic DNA analysis reveals that this gene has no intron.

关键词： Gossypium barbadense polygalacturonase-inhibiting protein ( pgip ) gene rapid amplification of cDNA ends (RACE) sequence analysis leucine-rich repeats (LRRs) .

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同源四倍体水稻的生殖特性研究

中国农业科学 1999年第2期32卷 16-19+115页

作者：黄群策孙敬三白素兰中国科学院植物研究所生殖室湘潭师范学院

与相应的二倍体水稻相比，同源四倍体的生殖特性发生了明显的变化，有性生殖能力明显变弱。在其花药内正常花粉粒数比较少（１５．６９％～２９．９８％），而败育花粉粒数比较多，它们的自交结实率（１７．７１％～３１．９５％）明显... 详细信息

与相应的二倍体水稻相比，同源四倍体的生殖特性发生了明显的变化，有性生殖能力明显变弱。在其花药内正常花粉粒数比较少（１５．６９％～２９．９８％），而败育花粉粒数比较多，它们的自交结实率（１７．７１％～３１．９５％）明显下降。ＡＰＩＶ（４）正常胚囊（４３．０％）数少，而退化胚囊（３６．０％）和变异胚囊（２１．０％）数多。ＡＰＩＶ（４）的花粉管在其花柱中的伸入速度明显变慢，双受精频率下降而单受精频率增加，在其自然群体中存在着胚自发现象。同源四倍体水稻有性生殖的异常可能为无融合生殖基因的表达创造了条件。

关键词：同源四倍体水稻生殖特性

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同源四倍体水稻雌雄配子体多态性的研究

杂交水稻 1999年第1期14卷 32-33,35页

作者：黄群策孙敬三白素兰湖南省湘潭师范学院中国科学院植物研究所生殖生物学室

同源四倍体水稻（２ｎ＝４ｘ＝４８）由于其细胞内染色体数的倍增而导致其生殖性状发生了明显的变化。在其雄配子体中存在着比较少的正常花粉粒（１３．３５％～１７．４９％）和比较多的败育花粉粒（８２．５１％～８６．６５％）。在... 详细信息

同源四倍体水稻（２ｎ＝４ｘ＝４８）由于其细胞内染色体数的倍增而导致其生殖性状发生了明显的变化。在其雄配子体中存在着比较少的正常花粉粒（１３．３５％～１７．４９％）和比较多的败育花粉粒（８２．５１％～８６．６５％）。在败育花粉粒中包括典败、园败和染败花粉粒。在同源四倍体水稻ＡＰＩＶ（４）的成熟胚囊内存在着正常蓼型胚囊（４３．０％）、退化型胚囊（３６．０％）和变异型胚囊（２１．０％）。在变异型胚囊中包括双卵卵器胚囊（７１．４％）、三卵卵器胚囊（１７．５％）和无助细胞胚囊（１１．１％）。在不同季节条件下ＡＰＩＶ（４）颖花内的多卵频率存在着明显差异，在同一季节条件下强势颖花的多卵频率要明显地高于弱势颖花的多卵频率。由此认为，同源四倍体水稻的雌雄配子体表现出异常发育状态，其有性生殖能力已明显变弱。

关键词：同源四倍体水稻雌配子体雄配子体多态性

Cloning of plastid division gene GlFtsZ from Gentiana lutea and its expression during petal development

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Progress in Natural Science:Materials International 2002年第8期 34-39+84页

作者： HE Yikun , ZHU Changfu , WANG Dong, KONG Dongdong and sun jingsan(1. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2. Department of Biology, Capital Normal University, Beijing 100037, China 3. College of Life Science, Northeast Normal University, Changchun 130024, China) Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China Department of Biology，Capital Normal University，Beijing 100037，China College of Life Science，Northeast Normal University，Changchun 130024，China Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China

A full-length cDNA of GlFtsZ was isolated by screening the cDNA library of Gentiana lutea. Analysis of the deduced amino acid sequence encoded by GIFtsZ indicated that GIFtsZ protein possesses the typical conservative motifs existed in all FtsZ proteins. The existence of putative plastid transit peptide in its N-terminus suggested that GIFtsZ might function inside of plastids. With the developmental process of petals of Gentiana lutea, the expression of plastid division gene GIFtsZ declined gradually, whereas the expression of carotenoids biosynthesis gene Zds increased obviously; meanwhile, in contrast to the increment of carotenoids, the content of chlorophyll in petals decreased sharply. The chloroplasts turned into chromoplasts, and the color of petals also turned from green to golden. All of these results suggested that the expression of GIFtsZ is accompanied with the development and differentiation of plastids.

关键词： FtsZ gene plastids chromoplasts Gentiana lutea .

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谷子体细胞无性系变异分析

中国农业科学 1999年第3期32卷 23-24+26-28页

作者：刁现民段胜军陈振玲智慧赵连元孙敬三中国科学院植物研究所河北省农林科学院谷子研究所

对谷子（ＳｅｔａｒｉａｉｔａｌｉｃａＢｅａｕｖ．）品种郑４０７幼穗外植体不定芽途径再生植株和幼穗愈伤组织再生植株后代的性状变异进行了研究分析。结果表明：无性系变异发生在愈伤组织形成和继代过程中；Ｒ２代变异频率达１０％... 详细信息

对谷子（ＳｅｔａｒｉａｉｔａｌｉｃａＢｅａｕｖ．）品种郑４０７幼穗外植体不定芽途径再生植株和幼穗愈伤组织再生植株后代的性状变异进行了研究分析。结果表明：无性系变异发生在愈伤组织形成和继代过程中；Ｒ２代变异频率达１０％，包括质量性状变异和农艺数量性状变异，变异具有高的遗传力和育种应用价值；Ｒ１代表现半不育或高度不育的单株，其Ｒ２代出现变异的频率高于Ｒ１代结实正常的单株。

关键词：谷子体细胞无性系变异分析

Cloning of two plastid division ftsZ genes from Nicotiana tabacum and their expression in E. coli

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Progress in Natural Science:Materials International 2002年第12期 31-36页

作者： WANG Dong KONG Dongdong HU Yong JU Chuanli HE Yikun and sun jingsan(1. Department of Biology, Capital Normal University, Beijing 100037, China 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 3. College of Life Sciences, Beijing Normal University, Beijing 100875, China) Department of Biology，Capital Normal University，Beijing 100037，China College of Life Sciences，Beijing Normal University，Beijing 100875，China Department of Biology，Capital Normal University，Beijing 100037，China Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China Department of Biology，Capital Normal University，Beijing 100037，China Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China

Two cDNAs of plastid division gene NtFtsZ1-1 and NtFtsZl-2 are isolated from Nicotiana tabacum by RT-PCR and rapid amplification cDNA ends (RACE) method. Analysis of the deduced amino acid sequences encoded by NtFtsZl-1 and NtFtsZl -2 indicate that these two proteins possess the typical conservative motifs and GTP binding sites existing in all FtsZ proteins. The existence of putative plastid transit peptide in their N-terminal suggests that there are at least two transit-peptide containing FtsZ proteins in higher plants. Phylogenetic analysis based on amino acid sequences of FtsZ proteins also supports this interference. These two NtFtsZ genes demonstrate a similar expression pattern during the plant development detected by Northern blot. Expression of NtFtsZl-1 and NtFtsZl-2 in E. coli interrupts the normal division process of host cells. These results suggest the diverse functions of FtsZ proteins in higher plants.

关键词： NtFtsZ genes plastld division Nicotiana tabacum plastid transit peptide expression in E.coli.