Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter

作     者：Guocun Huang Yuemei Dong Jingsan Sun 

作者机构：Chinese Acad Sci Inst Bot Beijing 100093 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (科学通报(英文版))

年 卷 期：1999年第44卷第8期

页      面：698-701页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：green fluorescent protein (GFP) pollen-tube pathway cotton. 

摘      要：The green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling has been verified according to its GFP-related fluorescence and Southern blotting analysis. The results provided direct and convincing facts in cytology and molecular biology for the pollen-tube pathway method, an efficient transformation technique used in plants.