Cloning and sequence analysis of a gene encoding polygalacturonase-inhibiting protein from cotton

作     者：DOU Daolong, WANG Bingshan, TANG Yixiong, WANG Zhixing and SUN Jingsan ( Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China) 

作者机构：Institute of Botany，Chinese Academy of Sciences，Beijing 100093 China Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2003年第2期

页      面：41-46页

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30170637) Research and Industrialization of Transgenic Plants (Grant No. ZJY-A-01) 

主　　题：Gossypium barbadense polygalacturonase-inhibiting protein ( pgip ) gene rapid amplification of cDNA ends (RACE) sequence analysis leucine-rich repeats (LRRs) . 

摘      要：Abstract Polygalacturonase- inhibiting proteins (PGIP) play important roles in plant defense of pathogen, especially fungi. A pairof degenerated primers is designed based on the conserved sequence of 20 other known pgip genes and used to amplify Gossypium barbadense cultivation 7124 cDNA library by touch-down PCR. A 561 bp internal fragment of the pgip gene is obtained and used to designthe primers for rapid amplification of cDNA ends. A composite pgip gene sequence is constructed from the products of 5 and 3 RACE,which are 666 bp and 906 bp respectively. Analysis of nucleic acid sequence shows 69.2% and 68. 7% similarity to Citrus and Ponciruspgip genes, respectively. Its open reading frame of the gene encodes a polypeptide of 330 amino acids, in which 10 leucine-rich repeats ar-range tandemly. A new set of primers is designed to the 5 and 3 ends of the gene, which allows amplification of the full-length gene fromthe cotton cDNA library. Genomic DNA analysis reveals that this gene has no intron.