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Brassinosteroids modulate nitrogen physiological response and promote nitrogen uptake in maize(Zea mays L.)

The Crop Journal 2022年第1期10卷 166-176页

作者： jiapeng xing Yubin Wang Qingqing Yao Yushi Zhang Mingcai Zhang Zhaohu Li State Key Laboratory of Plant Physiology and Biochemistry College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Crop Research Institute Shandong Academy of Agricultural SciencesJinan 250100ShandongChina

Brassinosteroids(BRs)are steroid hormones that function in plant growth and development and response to environmental stresses and nutrient ***,few studies have investigated the effect of BRs in modulating the physiological response to nitrogen(N)supply in *** the present study,BR signalingdeficient mutant zmbri1-RNAi lines and exogenous application of 2,4-epibrassinolide(e BL)were used to study the role of BRs in the regulation of physiological response in maize seedlings supplied with *** application of e BL increased primary root length and plant biomass,but zmbri1 plants showed shorter primary roots and less plant biomass than wild-type plants under low N(LN)and normal N(NN)*** induced the expression of the BR signaling-associated genes Zm DWF4,Zm CPD,Zm DET2,and Zm BZR1 and the production of longer primary roots than *** of Zm BRI1 weakened the biological effects of LN-induced primary root elongation.e BL treatment increased N accumulation in shoots and roots of maize seedlings exposed to LN or NN ***,zmbri1 plants showed lower N accumulation in shoots and roots than wild-type *** with reduced N accumulation,zmbri1 plants showed lower NO3-fluxes and^(15)NO_(3)^(-)*** expression of nitrate transporter(NRT)genes(Zm NPF6.4,Zm NPF6.6,Zm NRT2.1,Zm NRT2.2)was lower in zmbri1 than in wild-type roots,but e BL treatments up-regulated the transcript expression of NRT ***,BRs modulated N physiological response and regulated the transcript expression of NRT genes to promote N uptake in maize.

关键词： Brassinosteroids Nitrogen uptake Nitrate transporter gene Root architecture Maize

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Ethylene accelerates maize leaf senescence in response to nitrogen deficiency by regulating chlorophyll metabolism and autophagy

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The Crop Journal 2024年第5期12卷 1391-1403页

作者： jiapeng xing Ying Feng Yushi Zhang Yubin Wang Zhaohu Li Mingcai Zhang State Key Laboratory of Plant Environmental Resilience College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina

Leaf senescence is an orderly and highly coordinated process,and finely regulated by ethylene and nitrogen(N),ultimately affecting grain yield and nitrogen-use efficiency(NUE).However,the underlying regulatory mechanisms on the crosstalk between ethylene-and N-regulated leaf senescence remain a mystery in *** this study,ethylene biosynthesis gene ZmACS7 overexpressing(OE-ZmACS7)plants were used to study the role of ethylene regulating leaf senescence in response to N deficiency,and they exhibited the premature leaf senescence accompanied by increased ethylene release,decreased chlorophyll content and F_v/F_m ratio,and accelerated chloroplast ***,we investigated the dynamics changes of transcriptome reprogramming underlying ethylene-accelerated leaf senescence in response to N *** differentially expressed genes(DEGs)involved in chlorophyll biosynthesis were significantly down-regulated,while DEGs involved in chlorophyll degradation and autophagy processes were significantly up-regulated,especially in OE-ZmACS7 plants in response to N deficiency.A gene regulatory network(GRN)was predicted during ethylene-accelerated leaf senescence in response to N *** transcription factors(TFs)ZmHSF4,Zmb HLH106,and ZmEREB147 were identified as the key regulatory genes,which targeted chlorophyll biosynthesis gene ZmLES22,chlorophyll degradation gene ZmNYC1,and autophagy-related gene ZmATG5,***,ethylene signaling key genes might be located upstream of these TFs,generating the signaling cascade networks during ethylene-accelerated leaf senescence in response to N ***,these findings improve our molecular knowledge of ethylene-accelerated maize leaf senescence in response to N deficiency,which is promising to improve NUE by manipulating the progress of leaf senescence in maize.

关键词： Ethylene Leaf senescence N deficiency Chlorophyll metabolism Autophagy Gene regulatory network

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基于知识工程的船舶机舱设备三维布局设计

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上海交通大学学报 2021年第10期55卷 1219-1227页

作者：王运龙姜云博管官邢佳鹏于光亮大连理工大学船舶工程学院辽宁大连116024

船舶机舱设备布局设计属于密闭有限空间多目标优化设计问题,作为船舶的心脏,机舱设备的布局效果会影响到整条船舶各方面的性能.通过CATIA软件和知识工程技术对一艘多用途载货船舶机舱设备进行三维布局设计研究,建立船舶机舱分类规则以... 详细信息

船舶机舱设备布局设计属于密闭有限空间多目标优化设计问题,作为船舶的心脏,机舱设备的布局效果会影响到整条船舶各方面的性能.通过CATIA软件和知识工程技术对一艘多用途载货船舶机舱设备进行三维布局设计研究,建立船舶机舱分类规则以提高知识获取效率;建立设备虚拟面积增加规则来控制设备间距;运用参数化装配完成知识推理.最后,对生成的布局方案通过横倾力矩、干涉检查及逃生时间3项规则进行专家校验,结果符合预期,验证了知识工程在船舶机舱三维布局设计中的可行性和有效性.

关键词：船舶机舱三维布局知识工程虚拟面积多目标优化

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桥梁预防性养护效果-成本-寿命集成优化

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重庆交通大学学报（自然科学版） 2014年第4期33卷 24-28页

作者：王巍张春霞邢佳鹏乔国栋交通运输部公路科学研究院北京100088

应用多元回归和微粒群算法,构建了桥梁预防性养护效果、养护成本、耐久性寿命延长这3大目标综合集成控制的优化模型;建立了桥梁预防性养护全寿命周期费用模型和基于构件劣化的桥梁预防性养护耐久性寿命延长及养护周期数学模型;运用SPSS... 详细信息

应用多元回归和微粒群算法,构建了桥梁预防性养护效果、养护成本、耐久性寿命延长这3大目标综合集成控制的优化模型;建立了桥梁预防性养护全寿命周期费用模型和基于构件劣化的桥梁预防性养护耐久性寿命延长及养护周期数学模型;运用SPSS计算出养护效果对耐久性寿命延长年限和养护成本的多元回归方程,利用微粒群算法求得最优解,实现了桥梁预防性养护这3大目标的集成控制。实际工程验证表明:研究结果可为桥梁预防性养护时机和成本的选择提供参考和借鉴。

关键词：桥梁工程桥梁预防性养护效果-成本-寿命优化

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超声支气管镜引导下经支气管肺活检术对肺周围型病变的诊断价值

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中国医药科学 2022年第18期12卷 152-155页

作者：林武强邢佳鹏林岱敏广东省揭阳市人民医院呼吸与危重症医学科广东揭阳522000

目的探析在肺周围型病变诊断中应用超声支气管镜引导下经支气管肺活检术(EBUS-TBLB)的临床价值。方法2020年1月至2021年10月选取揭阳市人民医院呼吸与危重症医学科收治的80例肺周围型病变患者作为研究对象,将入选的患者按照随机数表法... 详细信息

目的探析在肺周围型病变诊断中应用超声支气管镜引导下经支气管肺活检术(EBUS-TBLB)的临床价值。方法2020年1月至2021年10月选取揭阳市人民医院呼吸与危重症医学科收治的80例肺周围型病变患者作为研究对象,将入选的患者按照随机数表法分为观察组和对照组,每组各40例,观察组患者应用EBUS-TBLB行经支气管肺活检,对照组患者采用在CT引导下行经皮肺穿刺术(CT-PNB)获取肺组织标本,然后比较两组患者在病理确诊阳性率、并发症发生率、费用方面的差异。结果观察组40例患者中34例成功获得标本且明确诊断,诊断阳性率为85.00%;对照组40例患者中25例成功获得标本且明确诊断,诊断阳性率为62.50%,差异有统计学意义(P0.05)。40例经超声小探头引导下经支气管镜肺活检患者中,34例患者成功检出肺周围型病变,其余6例患者未能检出。病灶直径>2 cm的阳性检出率为92.00%(23/25)高于病灶直径≤2 cm的73.33%(11/15)(χ^(2)=0.961,P=0.327),探头被病灶包绕的阳性检出率为92.31%(24/26),高于不被病灶包绕的71.43%(10/14)(χ^(2)=1.167,P=0.280)。结论在肺周围型病变诊断中,EBUS-TBLB的应用可以使诊断阳性率提高,而且还可以减少并发症的发生率,具备良好的安全性,诊断价值显著。

关键词：超声支气管镜经支气管肺活检术肺周围型病变并发症

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肝动脉化疗栓塞联合射频消融治疗中晚期肝癌的效果及安全性分析

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中外医学研究 2019年第33期17卷 55-57页

作者：邢佳鹏洪晓涛吴义波揭阳市人民医院

目的:研究肝动脉化疗栓塞(TACE)联合射频消融(RFA)对中晚期肝癌患者的治疗效果及安全性。方法:选取2017年4月-2018年4月在笔者所在医院接受治疗的30例中晚期肝癌患者为研究对象,随机分为两组,对照组行肝动脉化疗栓塞治疗,研究组行肝动... 详细信息

目的:研究肝动脉化疗栓塞(TACE)联合射频消融(RFA)对中晚期肝癌患者的治疗效果及安全性。方法:选取2017年4月-2018年4月在笔者所在医院接受治疗的30例中晚期肝癌患者为研究对象,随机分为两组,对照组行肝动脉化疗栓塞治疗,研究组行肝动脉化疗栓塞治疗后30 d内再行射频消融治疗;以两组治疗后1、3个月的近期疗效、术后血清AFP水平、不良反应为观察指标,考察治疗有效性与安全性。结果:术后3个月,研究组的ORR、DCR显著优于对照组(P0.05)。结论:TACE联合RFA治疗中晚期肝癌患者具有良好的临床疗效与安全性。

关键词：中晚期肝癌肝动脉化疗栓塞射频消融术

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Exploring the potential of extrachromosomal DNA as a novel oncogenic driver

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Science China Life Sciences 2025年第1期 144-157页

作者： Huanbo Zhu Longtao Huangfu Junbing Chen Jiafu Ji Xiaofang xing Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Division of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital & Institute Department of Gastrointestinal Cancer Center Peking University Cancer Hospital & Institute Department of Liver Surgery & Transplantation Liver Cancer InstituteZhongshan HospitalFudan University Key Laboratory of Carcinogenesis and Cancer Invasion Ministry of Education State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational ResearchDivision of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital & Institute

Extrachromosomal DNA(ecDNA) is a form of circular DNA mostly found in tumor cells. Unlike the typical chromosomal DNA, ecDNA is circular, self-replicating, and carries complete or partial gene fragments. Although ecDNA occurrence remains a rare event in cancer, recent studies have shown that oncogene amplification on ecDNA is widespread throughout many types of cancer, implying that ecDNA plays a central role in accelerating tumor evolution. ecDNA has also been associated with increased tumor mutation burden, chromosomal instability, and even tumor microenvironment remodeling. ecDNA may be crucial in influencing tumor heterogeneity, drug sensitivity,oncogenic senescence, and tumor immunogenicity, leading to a worsening prognosis for tumor patients. In this way, several clinical trials have been conducted to investigate the importance of ecDNA in clinical treatment. In this review, we summarize the biogenesis, characteristics, and current research methods of ecDNA, discuss the vital role of ecDNA-caused tumor heterogeneity in cancers, and highlight the potential role of ecDNA in cancer therapy.

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Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

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Nano-Micro Letters 2025年第1期17卷 281-292页

作者： xing Wang Wei Pi Sheng Hu Haifeng Bao Na Yao Wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

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A low redox potential and long life organic anode material for sodium-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 557-564页

作者： Zhi Li Yang Wei Kang Zhou Xin Huang xing Zhou Jie Xu Taoyi Kong Junwei Lucas Bao Xiaoli Dong Yonggang Wang Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New Energy Collaborative Innovation Center of Chemistry for Energy Materials (iChEM) Fudan University Department of Chemistry Boston College School of Materials Science and Engineering Anhui University of Technology

Sodium-ion batteries （SIBs） with organic electrodes are an emerging research direction due to the sustainability of organic materials based on elements like C,H,O,and sodium ***,organic electrode materials for SIBs are mainly used as cathodes because of their relatively high redox potentials（>1 V）.Organic electrodes with low redox potential that can be used as anode are ***,a novel organic anode material （tetrasodium 1,4,5,8-naphthalenetetracarboxylate,Na4TDC） has been developed with low redox potential （<0.7 V） and excellent cyclic *** three-sodium storage mechanism was demonstrated with various in-situ/ex-situ spectroscopy and theoretical calculations,showing a high capacity of 208 mAh/g and an average decay rate of merely 0.022%per ***,the Na4TDC-hard carbon composite can further acquire improved capacity and cycling stability for 1200 cycles even with a high mass loading of up to 20 mg *** pairing with a thick Na3V2（PO4）3cathode (20.6 mg cm-2),the as-fabricated full cell exhibited high operating voltage （2.8 V）,excellent rate performance and cycling stability with a high capacity retention of 88.7% after 200 cycles,well highlighting the Na4TDC anode material for SIBs.

关键词： Organic anode material Low redox potential Composite anode Sodium-ion batteries

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肝动脉灌注化疗对原发性肝癌合并门静脉癌栓的效果

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中国卫生标准管理 2022年第12期13卷 109-112页

作者：吴义波吴卓琼黄洁丽邢佳鹏洪晓涛揭阳市人民医院肿瘤内二科广东揭阳522000

目的探究肝动脉灌注化疗对原发性肝癌并门静脉癌栓的治疗效果。方法文章研究在本院肿瘤科2016年1月—2021年3月期间收治的患者群体内,随机选取50例确诊为原发性肝癌并门静脉癌栓并接受肝动脉灌注化疗治疗的患者作为此次研究的受试者。... 详细信息

目的探究肝动脉灌注化疗对原发性肝癌并门静脉癌栓的治疗效果。方法文章研究在本院肿瘤科2016年1月—2021年3月期间收治的患者群体内,随机选取50例确诊为原发性肝癌并门静脉癌栓并接受肝动脉灌注化疗治疗的患者作为此次研究的受试者。所有患者都行肝动脉灌注化疗,应用奥沙利铂、亚叶酸钙注射液、5-Fu行肿瘤供血动脉的灌注治疗,结果35例患者的门静脉癌栓治疗有效,占比为70%,其生存期最短为7.5个月,最长为26.0个月,平均为(13.52±3.39)个月;15例患者的门静脉癌栓治疗无效,占比为30%,其生存期最短为3.1个月,最长为12.5个月,平均为(4.01±1.08)个月,与治疗有效患者的生存时间差异有统计学意义(P<0.05);对照组治疗有效率为48%,观察组治疗有效率为70%,两组临床疗效差异有统计学意义(P<0.05);观察组并发症发生率为0,对照组并发症发生率为20%,两组并发症发生率差异有统计学意义(P<0.05)。结论对于原发性肝癌门静脉癌栓患者,肝动脉灌注化疗治疗的效果较好,并且较为安全,因此临床应用价值高。

关键词：原发性肝癌门静脉癌栓肝动脉灌注化疗疗效分析生存期并发症

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