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Efficacy of Huangma Ding or autologous platelet-rich gel for the diabetic lower extremity arterial disease patients with foot ulcers

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World Journal of Diabetes 2024年第5期15卷 923-934页

作者： Xue-Qin wang Dan-lan Pu Wei-Ling Leng Xiao-Tian Lei jiang Juan Zou La Ding Yao Jia-Zhuang Xi Li jian Teng Miao Qi-Nan Wu Endocrinology Department The People’s Hospital of Yubei District of Chongqing CityChongqing 401120China Department of Endocrinology Chongqing Yubei District People’s HospitalChongqing 400030China Endocrinology Department The First Affiliated Hospital of the Army Medical UniversityChongqing 400038China Endocrinology and Nephrology Department Chongqing University Cancer Hospital and Chongqing Cancer Institute and Chongqing Cancer HospitalChongqing 400016China Department of Endocrinology Dazu Hospital of Chongqing Medical UniversityThe People’s Hospital of DazuChongqing 402360China

BACKGROUND Diabetes foot is one of the most serious complications of diabetes and an important cause of death and disability,traditional treatment has poor efficacy and there is an urgent need to develop a practical treatment *** To investigate whether Huangma Ding or autologous platelet-rich gel(APG)treatment would benefit diabetic lower extremity arterial disease(LEAD)patients with foot *** A total of 155 diabetic LEAD patients with foot ulcers were enrolled and divided into three groups:Group A(62 patients;basal treatment),Group B(38 patients;basal treatment and APG),and Group C(55 patients;basal treatment and Huangma Ding).All patients underwent routine follow-up visits for six *** follow-up,we calculated the changes in all variables from baseline and determined the differences between groups and the relationships between *** The infection status of the three groups before treatment was the ***(PCT)improved after APG and Huangma Ding treatment more than after traditional treatment and was significantly greater in Group C than in Group *** regression analysis revealed that PCT was positively correlated with total amputation,primary amputation,and minor amputation *** ankle-brachial pressure and the transcutaneous oxygen pressure in Groups B and C were greater than those in Group *** major amputation rate,minor amputation rate,and total amputation times in Groups B and C were lower than those in Group *** Our research indicated that diabetic foot ulcers(DFUs)lead to major amputation,minor amputation,and total amputation through local infection and poor microcirculation and *** Ding and APG were effective attreating *** clinical efficacy of Huangma Ding was better than that of autologous platelet gel,which may be related to the better control of local infection by Huangma *** finding suggested that in patients with DFUs combined with coinfection,controll

关键词： Diabetic foot ulcer Huangma Ding Autologous platelet-rich gel Ankle-brachial index Transcutaneous oxygen partial pressure

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Novel MIP gene mutation causes autosomal-dominant congenital cataract

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 454-465页

作者： Jing-lan Ni Hua-Ming Wen Xiao-Sheng Huang Qian-Wen Li Jia-Min Cai Bao-jian Fan Jun Zhao The Second Clinical Medical College Jinan UniversityShenzhen 518020Guangdong ProvinceChina Department of Ophthalmology Dongguan Chang’an HospitalDongguan 523843Guangdong ProvinceChina Shenzhen Eye Hospital Affiliated to Jinan University Shenzhen Eye InstituteShenzhen 518040Guangdong ProvinceChina Department of Oral&Maxillofacial Surgery Shenzhen Stomatology Hospital Affiliated to Shenzhen UniversityShenzhen 518040Guangdong ProvinceChina Department of Neurosurgery Massachusetts General Hospital and Harvard Medical SchoolBoston 02114MassachusettsUSA Department of Ophthalmology Shenzhen People’s Hospital(the Second Clinical Medical CollegeJinan Universitythe First Affiliated HospitalSouthern University of Science and Technology)Shenzhen 518020Guangdong ProvinceChina

●AIM:To identify disease-causative mutations in families with congenital cataract.●METHODS:Two Chinese families with autosomaldominant congenital cataract(ADCC)were recruited and underwent comprehensive eye *** panel next-generation sequencing of common pathogenic genes of congenital cataract was performed in the proband of each *** sequencing was used to valid the candidate gene mutations and sequence the other family members for co-segregation *** effect of sequence changes on protein structure and function was predicted through bioinformatics *** intrinsic protein(MIP)-wildtype and MIP-G29R plasmids were constructed and microinjected into zebrafish single-cell stage *** embryonic lens phenotypes were screened using confocal microscopy.●RESULTS:A novel heterozygous mutation(c.85G>A;p.G29R)in the MIP gene was identified in the proband of one family.A known heterozygous mutation(c.97C>T;p.R33C;rs864309693)in MIP was found in the proband of another ***-silico prediction indicated that the novel mutation might affect the MIP protein *** embryonic lens was uniformly transparent in both wild-type PCS2+MIP and mutant PCS2+MIP.●CONCLUSION:Two missense mutations in the MIP gene in Chinese cataract families are identified,and one of which is *** findings expand the genetic spectrum of MIP mutations associated with *** functional studies suggest that the novel MIP mutation might not be a gain-of-function but a loss-of-function mutation.

关键词： congenital cataract major intrinsic protein missense mutation zebrafish model

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Analyzing the surface passivity effect of germanium oxynitride:a comprehensive approach through first principles simulation and interface state density

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Nuclear Science and Techniques 2024年第5期35卷 74-84页

作者： Sheng-Jie Du Xiu-Xia Li Yang Tian Yuan-Yuan Liu Ke Jia Zhong-Zheng Tang jian-Ping Cheng Zhi Deng Yu-lan Li Zheng-Cao Li Sha-Sha Lv College of Nuclear Science and Technology Joint Laboratory of Jinping Ultra‑Low Radiation Background Measurement of Ministry of Ecology and Environment Key Laboratory of Beam Technology of Ministry of Education Beijing Normal UniversityBeijing Normal UniversityBeijing 100875China Joint Research Center Nuctech Company LimitedBeijing 100084China Department of Engineering Physics Key Laboratory of Particle&Radiation Imaging of Ministry of EducationTsinghua UniversityBeijing 100084China Key Laboratory of Advanced Materials(MOE) School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China

High-purity germanium(HPGe)detectors,which are used for direct dark matter detection,have the advantages of a low threshold and excellent energy *** surface passivation of HPGe has become crucial for achieving an extremely low energy *** this study,first-principles simulations,passivation film preparation,and metal oxide semiconductor(MOS)capacitor characterization were combined to study surface *** calculations of the energy band structure of the -H,-OH,and -NH_(2) passivation groups on the surface of Ge were performed,and the interface state density and potential with five different passivation groups with N/O atomic ratios were accurately analyzed to obtain a stable surface *** on the theoretical calculation results,the surface passivation layers of the Ge_(2)ON_(2) film were prepared via magnetron sputtering in accordance with the optimum atomic ratio *** microstructure,C-V,and I-V electrical properties of the layers,and the passivation effect of the Al/Ge_(2)ON_(2)/Ge MOS were characterized to test the interface state *** mean interface state density obtained by the Terman method was 8.4×10^(11) cm^(-2) eV^(-1).The processing of germanium oxynitrogen passivation films is expected to be used in direct dark matter detection of the HPGe detector surface passivation technology to reduce the detector leakage currents.

关键词： Surface passivation High purity germanium detector Germanium nitrogen oxide Interface state density

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Pangenome and multi-tissue gene atlas provide new insights into the domestication and highland adaptation of yaks

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Journal of Animal Science and Biotechnology 2024年第5期15卷 1832-1850页

作者： Daoliang lan Wei Fu Wenhui Ji Tserang‑Donko Mipam Xianrong Xiong Shi Ying Yan Xiong Peng Sheng jiangping Ni Lijun Bai Tongling Shan Xiangdong Kong jian Li Ministry of Education of Key Laboratory of Qinghai‑Tibetan Plateau Animal Genetic Resource and Utilization Southwest Minzu UniversityChengduChina College of Animal&Veterinary Sciences Southwest Minzu UniversityChengduChina Institute of Qinghai‑Tibetan Plateau Southwest Minzu UniversityChengduChina Jiguang Gene Biotechnology Co. Ltd.NanjingChina Chengdu Genepre Technology Co. Ltd.ChengduChina Shanghai Veterinary Research Institute Chinese Academy of Agricultural SciencesShanghaiChina

Background The genetic diversity of yak,a key domestic animal on the Qinghai-Tibetan Plateau(QTP),is a vital resource for domestication and breeding *** study presents the first yak pangenome obtained through the de novo assembly of 16 yak *** We discovered 290 Mb of nonreference sequences and 504 new *** pangenome-wide presence and absence variation(PAV)analysis revealed 5,120 PAV-related genes,highlighting a wide range of variety-specific genes and genes with varying frequencies across yak *** component analysis(PCA)based on binary gene PAV data classified yaks into three new groups:wild,domestic,and ***,we pro-posed a‘two-haplotype genomic hybridization model'for understanding the hybridization patterns among breeds by integrating gene frequency,heterozygosity,and gene PAV data.A gene PAV-GWAS identified a novel gene(Bos-Gru3G009179)that may be associated with the multirib trait in Jinchuan ***,an integrated transcrip-tome and pangenome analysis highlighted the significant differences in the expression of core genes and the muta-tional burden of differentially expressed genes between yaks from high and low *** analysis across multiple species revealed that yaks have the most unique differentially expressed m RNAs and lnc RNAs(between high-and low-altitude regions),especially in the heart and lungs,when comparing high-and low-altitude *** The yak pangenome offers a comprehensive resource and new insights for functional genomic studies,supporting future biological research and breeding strategies.

关键词： High-and low-altitude Novel genes Pangenome PAV-GWAS Yak

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Spatial continuous modeling of early Cenozoic carbon cycle and climate

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National Science Review 2024年第4期11卷 30-32页

作者： Yonggang Liu jian Zhang Haoyue Zuo Zhilin He Jiaqi Guo Qifan Lin Haonan Yu Jiawenjing lan Jing Han Zhihong Song Zihan Yin Liang Zhao Yongyun Hu Zhengtang Guo Laboratory for Climate and Ocean-Atmosphere Studies Department of Atmospheric and Oceanic Sciences School of Physics Peking University Key Laboratory of Cenozoic Geology and Environment Institute of Geology and GeophysicsChinese Academy of Sciences State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences

The partial pressure of atmospheric CO2（pCO2） is determined by the carbon cycle that involves climate, biogeochemistry, and plate tectonics. In this cycle,atmospheric CO2is converted into organic carbon and carbonates by biological activities and silicate weathering, and buried as sediments. The sediments on the ocean floor are subducted into the Earth’s interior, and subsequently converted back into CO2and outgassed into the atmosphere.

关键词： ATMOSPHERIC CO2

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Medcheck:A novel software for automated de-formulation of traditional Chinese medicine(TCM)prescriptions by liquid chromatography-mass spectrometry

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Journal of Pharmaceutical Analysis 2024年第6期14卷 930-932页

作者： Xiao-lan Li jian-qing Zhang Yun Li Xuan-jing Shen Huan-ya Yang Lin Yang Meng Xu Qi-rui Bi Chang-liang Yao De-an Guo Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Research Center of TCM Standardization TechnologyShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China b University of Chinese Academy of Sciences Beijing100049China

Prescriptions are the main clinical application of traditional Chinese medicines(TCMs).Common forms include Chinese patent medicines,Kampo formulas,and hospital decoctions.A new pre-scription called“famous classical formulas”is recently developed and expected to boom in the *** constituent me-dicinal plants in prescriptions is critical for new drug development and quality control[1],which could avoid safety issues from adulteration or substandard ingredients,as seen in the notorious Longdan Xiegan Pill event.

关键词： medicines formulation prescription

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Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway

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Plant Communications 2024年第8期5卷 172-188页

作者： Hu-Jiao lan Jie Ran Wen-Xu wang Lei Zhang Ni-Ni Wu Ya-Ting Zhao Min-Jun Huang Min Ni Fen Liu Ninghui Cheng Paul A.Nakata jianwei Pan Steven A.Whitham Barbara J.Baker jian-Zhong Liu Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants Zhejiang Normal UniversityJinhua 321004China Institute of Plant Genetics and Developmental Biology College of Chemistry and Life SciencesZhejiang Normal UniversityJinhua 321004China College of Life Sciences Lanzhou UniversityLanzhou 730000China Department of Plant Pathology and Microbiology Iowa State UniversityAmesIA 50011USA Department of Plant and Microbial Biology University of CaliforniaBerkeleyBerkeleyCA 94720USA Plant Gene Expression Center U.S.Department of Agriculture-Agricultural Research ServiceAlbanyCA 94706USA Lushan Botanical Garden Chinese Academy of SciencesJiujiang 332000China U.S.Department of Agriculture-Agricultural Research Service Children’s Nutrition Research CenterDepartment of PediatricsBaylor College of MedicineHoustonTX 77030USA

The crosstalk between clathrin-mediated endocytosis(CME)and the autophagy pathway has been reported in mammals;however,the interconnection of CME with autophagy has not been established in ***,we report that the Arabidopsis CLATHRIN LIGHT CHAIN(CLC)subunit 2 and 3 double mutant,clc2-1 clc3-1,phenocopies Arabidopsis AUTOPHAGY-RELATED GENE(ATG)mutants in both autoimmunity and nutrient ***,the autophagy pathway is significantly compromised in the clc2-1 clc3-1 ***,multiple assays demonstrate that CLC2 directly interacts with ATG8h/ATG8i in a domain-specific *** expected,both GFP-ATG8h/GFP-ATG8i and CLC2-GFP are subjected to autophagic degradation,and degradation of GFP-ATG8h is significantly reduced in the clc2-1 clc3-1 ***,simultaneous knockout of ATG8h and ATG8i by CRISPR-Cas9 results in enhanced resistance against Golovinomyces cichoracearum,supporting the functional relevance of the CLC2-ATG8h/8i *** conclusion,our results reveal a link between the function of CLCs and the autophagy pathway in Arabidopsis.

关键词： autophagy clathrin light chain immunity clathrin-mediated endocytosis senescence cell death

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Risk factors for renal outcomes in children with antineutrophil cytoplasmic antibody-associated vasculitis:a nationwide retrospective study in China

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World Journal of Pediatrics 2024年第5期20卷 506-516页

作者： Li-Wen Tan Jun-Li Wan Chun-Hua Zhu Hong Xu Zheng-Kun Xia Li-Zhi Chen Xiao-Chuan Wu Fang wang Xiao-Rong Liu Cheng-Guang Zhao Xiao-Zhong Li jian-Hua Mao Xiao-Wen wang Wen-Yan Huang Yu-Hong Li jian-jiang Zhang Shi-Pin Feng Jun Yang Jiao-Jiao Liu Chun-Lin Gao Li-Ping Rong lan-Jun Shuai Ke Xu He-Jia Zhang Qiu Li Ai-Hua Zhang Mo wang Department of Nephrology Children’s Hospital of Chongqing Medical UniversityZhongshan 2nd Rd.136Chongqing400014China Ministry of Education Key Laboratory of Child Development and Disorders National Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChildren’s Hospital of Chongqing Medical UniversityChongqingChina Chongqing Key Laboratory of Pediatrics Children’s Hospital of Chongqing Medical UniversityChongqingChina Department of Nephrology Children’s Hospital of Nanjing Medical University72 Guangzhou RoadNanjing210008China Department of Nephrology Children’s Hospital of Fudan UniversityNational Paediatric Medical Center of ChinaShanghaiChina Department of Pediatrics Jinling HospitalNanjing Medical UniversityNanjingChina Department of Pediatrics Jinling HospitalThe First School of Clinical MedicineSouthern Medical UniversityNanjingChina Department of Pediatrics Jinling HospitalMedical School of Nanjing UniversityNanjingChina Department of Pediatric Nephrology and Rheumatology The First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouChina Department of Pediatrics The Second Xiangya Hospital of Central South UniversityChangshaChina Department of Pediatrics Peking University First HospitalBeijingChina Department of Nephrology Beijing Children’s HospitalCapital Medical UniversityBeijing100045China National Center for Children’s Health BeijingChina Department of Pediatrics Shengjing Hospital of China Medical UniversityShenyangChina Department of Nephrology and Immunology Children’s Hospital of Soochow UniversitySuzhouChina Department of Nephrology Children HospitalZhejiang University School of MedicineHangzhouChina Department of Nephrology Wuhan Children’s Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science&TechnologyWuhanChina Department of Nephrology and Rheumatology Shanghai Children’s HospitalSchool

Background Pediatric antineutrophil cytoplasmic antibody-associated vasculitis(AAV)is a life-threatening systemic vasculitis featured by liability to renal ***,there are few studies on the risk factors and predictive models for renal outcomes of AAV in *** Data from 179 AAV children in multiple centers between January 2012 and March 2020 were collected *** risk factors and predictive model of end-stage renal disease(ESRD)in AAV were *** Renal involvement was the most typical manifestation(95.5%),and the crescent was the predominant pathological lesion(84.9%).The estimated glomerular filtration rate(eGFR)was evaluated in 114 patients,of whom 59.6%developed ESRD,and the median time to ESRD was 3.20 *** eGFR[P=0.006,odds ratio(OR)=0.955,95%confidence interval(CI)=0.924–0.987]and the percentages of global glomerulosclerosis(pGGS;P=0.018,OR=1.060,95%CI=1.010–1.112)were independent risk factors for ESRD of renal *** on the pGGS and eGFR at renal biopsy,we developed three risk grades of ESRD and one predictive *** Kaplan‒Meier curve indicated that renal outcomes were significantly different in different risk grades(P<0.001).Compared with serum creatinine at baseline,the predictive model had higher accuracy(0.86 versus 0.58,P<0.001)and a lower coefficient of variation(0.07 versus 0.92)in external *** Renal involvement is the most common manifestation of pediatric AAV in China,of which more than half deteriorates into *** predictive model based on eGFR at renal biopsy and the pGGS may be stable and accurate in speculating the risk of ESRD in AAV children.

关键词： Antineutrophil cytoplasmic antibody End-stage renal disease Glomerulonephritis Pediatric nephrology Vasculitis

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撤县设区、空间一致性与县域经济发展

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中央财经大学学报 2024年第5期 115-128页

作者：王兰兰赵建梅中央财经大学创新发展学院中国公共财政与政策研究院中央财经大学创新发展研究院中国公共财政与政策研究院

县域经济是我国实现城镇化发展的主战场。本文利用夜间灯光数据代理县域经济发展水平,突破统计数据受制于行政区划的空间限制,获得空间一致且跨时间可比的县域经济衡量指标。这不仅可避免因使用统计数据可能导致的可塑性面积单元问题,... 详细信息

县域经济是我国实现城镇化发展的主战场。本文利用夜间灯光数据代理县域经济发展水平,突破统计数据受制于行政区划的空间限制,获得空间一致且跨时间可比的县域经济衡量指标。这不仅可避免因使用统计数据可能导致的可塑性面积单元问题,还可以同时分析“撤县设区”对县(市)和市辖区的影响,为“撤县设区”的研究提供更细致完整的图景。研究发现:“撤县设区”改革无论对被撤并县(市),还是对原市辖区的经济发展均未产生显著影响。原因在于,尽管“撤县设区”能够促进县域企业吸收更多人力资本,但却显著降低了企业的固定资产净投资和全要素生产率,这在一定程度上解释了为何“撤县设区”改革不能显著推动县域经济发展的原因。

关键词：撤县设区空间一致夜间灯光经济发展可塑性面积单元问题

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山葡萄VaERF095基因表达及其启动子功能分析

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南方农业学报 2024年第1期55卷 37-46页

作者：王岚许建韧宁夏大学葡萄酒与园艺学院宁夏银川750021 北方民族大学生物科学与工程学院宁夏银川750021

【目的】克隆山葡萄乙烯响应因子基因VaERF095及其启动子序列,分析在低温和外源激素诱导下该基因的表达模式和启动子活性,为深入探究VaERF095基因参与低温胁迫响应机理提供理论依据。【方法】采用同源克隆方法从山葡萄品种左山-1中获得V... 详细信息

【目的】克隆山葡萄乙烯响应因子基因VaERF095及其启动子序列,分析在低温和外源激素诱导下该基因的表达模式和启动子活性,为深入探究VaERF095基因参与低温胁迫响应机理提供理论依据。【方法】采用同源克隆方法从山葡萄品种左山-1中获得VaERF095基因及其启动子,并通过实时荧光定量PCR检测VaERF095基因在低温(4℃)胁迫和外源激素诱导下及不同组织中的表达情况。同时构建VaERF095基因启动子融合GUS蛋白表达载体,瞬时转化葡萄叶片,通过β-葡萄糖苷酸酶(GUS)活性定量分析VaERF095基因启动子受低温胁迫和外源激素诱导的表达情况。【结果】从山葡萄克隆获得VaERF095基因,编码区(CDS)全长393 bp,编码130个氨基酸残基,该蛋白含有1个保守的AP2/ERF结构域,与欧洲葡萄和河岸葡萄的亲缘关系较近,氨基酸序列相似性分别为100.00%和93.08%。Va ERF095基因可响应低温和外源激素乙烯(ET)、水杨酸(SA)、茉莉酸甲酯(MeJA)和脱落酸(ABA)的诱导,呈现出不同的表达模式。VaERF095基因在老叶、茎、花序、卷须和果实均有表达,其中在卷须中的表达量最高,在幼叶中的表达量极低或几乎不表达。克隆获得长度为1360 bp的VaERF095基因启动子,含有2个低温响应元件(LTR)、1个ET响应元件(ERE)、1个SA响应元件(TCA-element)、2个MeJA响应元件(CGTCA-motif和TGACG-motif)、1个ABA响应元件(ABRE)、1个GA响应元件(P-box)和1个防御和胁迫响应元件(TC-rich repeats)。在低温胁迫及外源激素(ET、SA和ABA)诱导下,VaERF095基因启动子活性较对照极显著升高(P<0.01),在MeJA诱导下,VaERF095基因启动子活性显著增强(P<0.05)。【结论】VaERF095基因及其启动子正向响应低温(4℃)及外源激素(ET、SA、ABA和MeJA)的诱导,具有明显的组织表达特异性。

关键词：山葡萄 ERF转录因子基因表达启动子功能分析

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