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The Role of the AMPK Pathway in ZEA-induced Cellcycle Arrest in Rat Sertoli Cells

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Biomedical and Environmental Sciences 2019年第12期32卷 944-948页

作者： CHEN Yu lian CHEN Yong Fang FENG Nan Nan LIU Zong Ping BIAN jian Chun College of Veterinary Medicine Yangzhou UniversityYangzhou 225009JiangsuChina jiangsu Co-innovation Center for Preve ntion and Control of Imports nt An imal In fectious Diseases and Zoonoses Yan gzhou 225009Jia ngsuChina

Zearalenone(ZEA),a non-steroidal estrogen-like mycotoxin biosynthesized by several Fusarium species through the polyketide pathway,is also known as the F-2 toxin[1].ZEA is one kind of common mycotoxins frequently found in feed ingredients and compound feed that can specifically and competitively bind to estrogen receptors resulting in sexual hormone disorder which can negatively affectthe expressi on of sec on dary sexual characteristics[2].

关键词： competitive cycle steroidal

固相萃取-高效液相色谱-串联质谱法检测动物组织和水产品中曲美他嗪含量

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粮食与油脂 2024年第10期37卷 147-151页

作者：汪春明霍江莲贾景建王美玲彭涛陈冬东中检科(北京)测试认证有限公司北京100176 中国合格评定国家认可中心北京100062 检科院(北京)科学技术有限公司北京100176 中国检验检疫科学研究院北京100176

建立通过固相萃取-高效液相色谱-串联质谱法测定动物组织和水产品中曲美他嗪的方法。样品经体积分数0.2%甲酸乙腈溶液涡旋超声提取,再经混合型强阳离子交换固相萃取柱Cleanert PCX净化,用3 mL体积分数3%氨水甲醇洗脱,净化液浓缩后用1 m... 详细信息

建立通过固相萃取-高效液相色谱-串联质谱法测定动物组织和水产品中曲美他嗪的方法。样品经体积分数0.2%甲酸乙腈溶液涡旋超声提取,再经混合型强阳离子交换固相萃取柱Cleanert PCX净化,用3 mL体积分数3%氨水甲醇洗脱,净化液浓缩后用1 mL初始流动相(乙腈与体积分数0.1%甲酸水体积比2∶8)复溶,经尼龙膜过滤上机检测;建立的方法采用InertSustain C18色谱柱、电喷雾正离子模式(ESI+)和多反应离子监测模式(MRM)检测,基质匹配内标法定量。结果表明:在动物组织和水产品基质中,曲美他嗪在0.3~10 ng/mL质量浓度范围内线性关系良好,决定系数均≥0.998,平均回收率为92.3%~109.6%,相对标准偏差为3.9%~12.5%。建立的方法可满足目标物检测的准确度、精密度和灵敏度要求。

关键词：曲美他嗪动物组织水产品检测

Prognostic value of circulating tumor cells in esophageal cancer

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World Journal of Gastroenterology 2017年第7期23卷 1310-1318页

作者： Hai-Tao Xu Jing Miao jian-Wei Liu lian-Guo Zhang Qing-Guang Zhang Department of Thoracic Surgery Affiliated Hospital of Binzhou Medical University Department of Neonatal Intensive Care Unit Binzhou People’s Hospital

AIM To perform a meta-analysis of the related studies to assess whether circulating tumor cells(CTCs) can be used as a prognostic marker of esophageal *** Pub Med, Embase, Cochrane Library and references in relevant studies were searched to assess the prognostic relevance of CTCs in patients with esophageal cancer. The primary outcome assessed was overall survival(OS). The meta-analysis was performed using the random effects model, with hazard ratio(HR), risk ratio(RR) and 95% confidence intervals(95%CIs) as effect *** Nine eligible studies were included involving a total of 911 esophageal cancer patients. Overall analyses revealed that CTCs-positivity predicted disease progression(HR = 2.77, 95%CI: 1.75-4.40, P 2.67, 95%CI: 1.99-3.58, P 2.06, P = 0.02) and T stage(RR = 1.44, 95%CI: 1.13-1.84, P = 0.003) in esophageal *** Detection of CTCs at baseline indicates poor prognosis in patients with esophageal cancer. However, this finding relies on data from observational studies and is potentially subject to selection bias. Prospective trials are warranted.

关键词： Circulating tumor cells Esophageal cancer Prognosis Meta-analysis

青岛市雾日PM_(1)中金属元素来源及健康风险评估

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中国环境科学 2024年第4期44卷 1945-1956页

作者：陶文鑫杜金花杨建立谭玉冉王超龙薛莲隋浩新张厚勇刘晓环张宜升青岛理工大学环境与市政工程学院山东青岛266520 山东省青岛生态环境监测中心山东青岛266003 山东省济南生态环境监测中心山东济南250102 中国海洋大学环境科学与工程学院山东青岛266100

为了解青岛市雾日PM_(1)中金属元素的污染程度及其来源,并评估其对人类健康的危害,依据能见度及湿度数据对雾日进行划分,结合正定矩阵因子分解法(PMF)源解析模型和健康风险评价模型研究青岛市雾日亚微米颗粒物(PM_(1))中金属元素的来源... 详细信息

为了解青岛市雾日PM_(1)中金属元素的污染程度及其来源,并评估其对人类健康的危害,依据能见度及湿度数据对雾日进行划分,结合正定矩阵因子分解法(PMF)源解析模型和健康风险评价模型研究青岛市雾日亚微米颗粒物(PM_(1))中金属元素的来源和健康风险.结果表明,清洁雾日PM_(1)浓度略高于清洁日,而污染雾日PM_(1)浓度是霾日PM_(1)的1.11倍,清洁日的3.07倍.秋冬季雾日金属元素受人为源影响,K元素含量最高;夏季雾日的主要贡献元素是典型的地壳元素Ca、Fe、Al及海盐Na元素.PMF结果表明秋冬季雾日PM_(1)中金属元素主要来自煤/生物质燃烧、机动车源、地壳源、海盐源、船舶源和工业源;夏季雾日PM_(1)中金属元素主要来自煤/生物质燃烧、机动车源、地壳源、海盐源、船舶源和工业源.夏季采样点位临海,海雾频发,海盐源为夏季雾日金属元素的重要贡献.健康风险评估结果表明,成年人与儿童暴露于青岛秋冬季雾日PM_(1)的非致癌风险均低于阈值.成人和儿童呼吸途径Mn的非致癌风险最高,儿童手口摄食As的非致癌风险(HQAS=0.50)最高.As(3.1×10^(-6))和Cr(1.9×10^(-6))的致癌风险均已超过致癌风险阈值,建议加强含Mn、As、Cr排放源的管控.

关键词： PM_(1) 金属元素雾日来源健康风险

Features of synchronous and metachronous dual primary gastric and colorectal cancer

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World Journal of Gastrointestinal Oncology 2023年第11期15卷 1864-1873页

作者： Yi-Jia Lin Hua-Xian Chen Feng-Xiang Zhang Xian-Sheng Hu Hai-Juan huang jian-Hua Lu Ye-Zi Cheng Jun-Sheng Peng Lei lian Department of General Surgery(Gastrointestinal Surgery) The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655Guangdong ProvinceChina Department of General Surgery(Colorectal Surgery) The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655Guangdong ProvinceChina Follow-up office The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655Guangdong ProvinceChina

BACKGROUND Studies evaluating the characteristics of dual primary gastric and colorectal cancer(CRC)(DPGCC)are *** To analyze the clinicopathologic characteristics and prognosis of synchronous and metachronous cancers in patients with *** From October 2010 to August 2021,patients with DPGCC were retrospectively *** patients with DPGCC were divided into two groups(synchronous and metachronous).We compared the overall survival(OS)between the groups using Kaplan-Meier survival *** and multivariate analyses were performed using Cox’s proportional hazards model to identify the independent prognostic factors for *** Of the 76 patients with DPGCC,46 and 30 had synchronous and metachronous cancers,*** proportion of unresectable CRC in patients with synchronous cancers was higher than that in patients with metachronous cancers(28.3%vs 3.3%,P=0.015).The majority of the second primary cancers had occurred within 5 ***-Meier survival analysis showed that the patients with metachronous cancers had a better prognosis than patients with synchronous cancers(P=0.010).The patients who had undergone gastrectomy(P2.0-22.7,(P=0.002)]and stage III-IV gastric cancer(GC)[HR=10.0,95%CI:3.4-29.5,(P2,95%CI:0.1-0.6,P=0.002].CONCLUSION Regular surveillance for metachronous cancer is necessary during postoperative *** resection is the mainstay of therapy to improve the prognosis of *** prognosis appears to be influenced by the stage of GC rather than the stage of *** with synchronous cancer have a worse prognosis,and its treatment strategy is worth further exploration.

关键词： Synchronous Metachronous Prognosis Gastric cancer Colorectal cancer

In-situ polymerization for mechanical strong composite actuators based on anisotropic wood and thermoresponsive polymer

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Chinese Chemical Letters 2022年第5期33卷 2635-2638页

作者： lian Chen Xianshuo Wei Feng Wang Shaoju jian Weisen Yang Chunxin Ma Gaigai Duan Shaohua jiang jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources International Innovation Center for Forest Chemicals and MaterialsCollege of Materials Science and EngineeringNanjing Forestry UniversityNanjing 210037China Fujian Key Laboratory of Eco-Industrial Green Technology College of Ecology and Resources EngineeringWuyi UniversityWuyishan 354300China Shandong Key Laboratory of Biochemical Analysis College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdao 266042China State Key Laboratory of Marine Resource Utilization in South China Sea Hainan UniversityHaikou 570228China

Stimuli-responsive hydrogels hold an irreplaceable statue in intelligent actuation materials because of their reversible stretchability and excellent ***,the poor mechanical performance and complicated fabrication process of anisotropic structures severely limit their further ***,we report a high-strength thermoresponsive wood-PNIPAM composite hydrogel actuator with complex deformations,through a simple in-situ *** this composite hydrogel actuator,the anisotropic wood and the thermoresponsive PNIPAM hydrogel hydroel can work together to pro-vide bending and even other complex *** to strong interfacial interaction,this actuator perfectly realized the combination of good mechanical properties(∼1.1 MPa)and fast actuation speed(∼0.9 s).In addition,by adjusting the orientation direction of wood,this actuator can achieve various complex *** composite hydrogel actuator could be a good candidate for intelligent appli-cations,such as intelligent actuators,smart valves,manipulators and even soft robots.

关键词： Actuator Anisotropic structure Thermoresponsive Wood

Effect of NDC80 in human hepatocellular carcinoma

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World Journal of Gastroenterology 2017年第20期23卷 3675-3683页

作者： Lin-Ling Ju Lin Chen Jun-Hong Li Yi-Fan Wang Ru-jian Lu Zhao-lian Bian jian-Guo Shao Nantong Institute of Liver Diseases Nantong Third People's HospitalNantong University Hematology Laboratory Affiliated Hospital of Nantong University

AIM To investigate the role of nuclear division cycle(NDC)80in human hepatocellular *** NDC80 gene expression was analyzed by real-time reverse transcription polymerase chain reaction in 47paired hepatocellular carcinoma(HCC)and adjacent *** HCC cell line SMMC-7721 was transfected with lentivirus to silence endogenous NDC80 gene expression,which was confirmed by real-time polymerase chain reaction and western *** effects of NDC80silencing on SMMC-7721 cell proliferation were evaluated by Cellomics Array Scan VTI *** cycle analysis and apoptosis were detected with flow *** formation was assessed by fluorescence *** NDC80 expression levels in HCC tissues were significantly higher than those in the adjacent *** studies demonstrated that NDC80 silencing significantly reduced SMMC-7721 cell proliferation and colony *** of NDC80 resulted in increased apoptosis and cell cycle arrest at ***80 contributed to HCC progression by reducing apoptosis and overcoming cell cycle arrest. CONCLUSION Elevated expression of NDC80 may play a role in promoting the development of HCC.

关键词： NDC80 Cell proliferation Apoptosis Cell cycle Hepatocellular carcinoma

Effect ofα-pinene on nuclear translocation of NF-κB in THP-1 cells

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Acta Pharmacologica Sinica 2004年第4期 82-86页

作者： jian-ya ZHOU1,2, Fa-di TANG1,3, Guo-gen MAO1, Ru-lian BIAN1 1Zhejiang Respiratory Drugs Research Laboratory of State Drugs Administration of China 2Departmentof Respiratory Medicine, First Affilated Hospital, Medical School of Zhejiang University, Hangzhou 310003 Medical School ofZhejiang University, Hangzhou 310031, China

AIM: To study the effects of α-pinene on nuclear translocation of nuclear factor-κB (NF-κB) and the expression of the inhibitor of NF-κB (IκBα) in human monocyte THP-1 cell line. METHODS: THP-1 cells were incubated with α-pinene (1, 10, and 100 mg/L, for 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg/L, 30 min). The location of NF-κB p65 subunit (NF-κB/p65) in THP-1 cells was detected by immunofluorescence and laser scanning confocal microscope (LSCM). The expression of NF-κB/p65 in nuclei and that of IκBα in cytoplasm were measured by Western-blot analysis. RESULTS: The majority of FITC-labelled NF-κB/p65 was located in the nuclei being stimulated with LPS. Whereas, no such fluorescence was seen in the nuclei of the groups pretreated with α-pinene or control cells. α-Pinene pretreatment decreased the NF-κB/p65 nuclear translocation in LPS- stimulated THP-1 cells, and this effect was dose-dependent, but there was no reaction in LPS-unstimulated THP-1 cells. α-Pinene pretreatment increased IκBα protein level in cytoplasm, compared with that in LPS-stimulated THP-1 cells. CONCLUSION: In a dose-related fashion, α-pinene inhibits the nuclear translocation of NF-κB induced by LPS in THP-1 cells, and this effect is partly due to the upregulation of IκBα expression.

关键词： alpha-pinene NF-kappa B THP-1 cells I-kappa B fluorescent antibody technique Western blot- ting

Selective sensing of Cr~Ⅵ and Fe~Ⅲ ions in aqueous solution by an exceptionally stable Tb~Ⅲ-organic framework with an AIE-active ligand

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Chinese Chemical Letters 2021年第8期32卷 2443-2447页

作者： Jing-Jing Pang Rui-Huan Du Xin lian Zhao-Quan Yao jian Xu Xian-He Bu School of Materials Science and Engineering National Institute for Advanced Materials Tianjin Key Laboratory of Metal and Molecule-Based Material Chemistry Nankai University State Key Laboratory of Elemento-Organic Chemistry College of Chemistry Nankai University

We herein report a new lanthanide metal-organic framework(MOF) that exhibits excellent chemical stability,especially in the aqueous solution over a wide pH range from 1 to *** contrast to many reported lanthanide MOFs,this Tb-based MOF emits cyan fluorescence inherited from the integrated AIEactive ligand,rather than Ln3+*** remarkably,its fluorescence signal features a highly selective and sensitive "turn-off" response toward CrO42-,Cr2O72-and Fe3+ions,highlighted with the low detection limits down to 68.18,69.85 and 138.8 ppm,***,the exceptional structural stability and sensing performance render this material able to be a superior luminescent sensor for heavy metal ions in wastewater.

关键词： Lanthanide metal-organic framework Aggregation-induced emission Heavy metal ions Selective luminescence sensing Aqueous-phase sensing