Effect ofα-pinene on nuclear translocation of NF-κB in THP-1 cells

作     者：Jian-ya ZHOU1,2, Fa-di TANG1,3, Guo-gen MAO1, Ru-lian BIAN1 1Zhejiang Respiratory Drugs Research Laboratory of State Drugs Administration of China 2Departmentof Respiratory Medicine, First Affilated Hospital, Medical School of Zhejiang University, Hangzhou 310003 Medical School ofZhejiang University, Hangzhou 310031, China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第4期

页      面：82-86页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：alpha-pinene NF-kappa B THP-1 cells I-kappa B fluorescent antibody technique Western blot- ting 

摘      要：AIM: To study the effects of α-pinene on nuclear translocation of nuclear factor-κB (NF-κB) and the expression of the inhibitor of NF-κB (IκBα) in human monocyte THP-1 cell line. METHODS: THP-1 cells were incubated with α-pinene (1, 10, and 100 mg/L, for 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg/L, 30 min). The location of NF-κB p65 subunit (NF-κB/p65) in THP-1 cells was detected by immunofluorescence and laser scanning confocal microscope (LSCM). The expression of NF-κB/p65 in nuclei and that of IκBα in cytoplasm were measured by Western-blot analysis. RESULTS: The majority of FITC-labelled NF-κB/p65 was located in the nuclei being stimulated with LPS. Whereas, no such fluorescence was seen in the nuclei of the groups pretreated with α-pinene or control cells. α-Pinene pretreatment decreased the NF-κB/p65 nuclear translocation in LPS- stimulated THP-1 cells, and this effect was dose-dependent, but there was no reaction in LPS-unstimulated THP-1 cells. α-Pinene pretreatment increased IκBα protein level in cytoplasm, compared with that in LPS-stimulated THP-1 cells. CONCLUSION: In a dose-related fashion, α-pinene inhibits the nuclear translocation of NF-κB induced by LPS in THP-1 cells, and this effect is partly due to the upregulation of IκBα expression.