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机器学习识别激素性股骨头坏死中线粒体自噬诊断标志物及免疫浸润分析

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中国组织工程研究 2025年第11期29卷 2402-2410页

作者：黄柯琪陈跃平陈尚桐李加根广西中医药大学附属瑞康医院广西壮族自治区南宁市530000

背景:线粒体自噬和激素性股骨头坏死的发生、发展关系密切,但具体生物标志物及调控机制尚未明确。目的:通过机器学习算法识别激素性股骨头坏死中线粒体自噬的关键标志物及免疫浸润分析。方法:从GEO数据库下载股骨头坏死数据集GSE123568... 详细信息

背景:线粒体自噬和激素性股骨头坏死的发生、发展关系密切,但具体生物标志物及调控机制尚未明确。目的:通过机器学习算法识别激素性股骨头坏死中线粒体自噬的关键标志物及免疫浸润分析。方法:从GEO数据库下载股骨头坏死数据集GSE123568和GSE74089,分别作为训练集和验证集,在激素性股骨头坏死组和对照组之间选择差异表达基因,进行加权共表达分析。从MitoCarta 3.0数据库下载线粒体自噬相关基因,然后与差异基因和模块基因取交集。利用两种机器学习算法鉴定激素性股骨头坏死线粒体自噬关键基因,利用外部验证集进行验证。采用CIBERSORT和免疫浸润分析免疫细胞占比,单样本基因集富集分析线粒体自噬基因与免疫细胞的相关性分析。结果与结论:①差异分析共获得1163个差异基因,其中有663个上调基因和500个下调基因;加权共表达分析鉴定出4个相关模块,共1412个模块基因;②最终与线粒体自噬基因取交集初步筛选出39个交叉基因可能是疾病相关线粒体自噬基因;GO富集分析结果显示,生物过程主要涉及血红素代谢、线粒体运输、核苷酸双磷酸代谢和硫酯代谢过程,细胞组分主要涉及线粒体基质、线粒体外膜、细胞器外膜和线粒体内膜,分子功能主要涉及脂肪酸连接酶活性、铁-硫簇结合和辅酶A连接酶活性;KEGG富集分析结果共映射出6条通路,主要涉及脂肪酸降解、线粒体自噬、丁酸代谢、脂肪酸生物合成和辅因子生物合成;③经LASSO回归和随机森林算法分析最终得到4个核心基因(ALDH5A1、FBXL4、MCL1和STOM),4个核心基因和诊断列线图外部验证集的受试者工作特征曲线均大于0.9;④激素性股骨头坏死发生发展与活化的树突状细胞、骨髓来源的抑制性细胞、调节性T细胞和中心记忆CD8T细胞等免疫细胞有关;⑤结果显示,4个关键的线粒体自噬基因ALDH5A1、FBXL4、MCL1和STOM通过破骨细胞分化和免疫机制在激素性股骨头坏死进展中发挥关键作用,均具有较好的疾病预测效果,可能作为激素性股骨头坏死诊断和治疗的生物标志物。

关键词：激素性股骨头坏死线粒体自噬机器学习免疫细胞浸润关键标志物

Regulation of drug release performance using mixed doxorubicin-doxorubicin dimer nanoparticles as a pH-triggered drug self-delivery system

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Journal of Pharmaceutical Analysis 2022年第1期12卷 122-128页

作者： jiagen li Xinming li Pengwei Xie Peng liu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou730000China

A mixed drug self-delivery system(DSDS)with high drug content(>50%)was developed to regulate pHtriggered drug release,based on two doxorubicin(DOX)-DOX dimmers:D-DOX_(ADH) and D-DOX_(car) conjugated with acid-labile dynamic covalent bonds(hydrazone and carbamate,respectively)and stabilized with PEGylated D-DOX_(ADH)(D-DOX_(ADH)-PEG).Owing to the different stability of the dynamic covalent bonds in the two dimers and the noncovalent interaction between them,pH-triggered drug release could be easily regulated by adjusting the feeding ratios of the two DOX-DOX dimers in the mixed *** in vitro cellular toxicity was achieved with the mixed DSDS nanoparticles prepared with different feeding *** mixed DSDS nanoparticles had a similar DOX content and diameter but different drug releasing *** MTT assays revealed that a high anti-tumor efficacy could be achieved with the slowrelease mixed DSDS nanoparticles.

关键词： Drug self-delivery system Regulated drug release Doxorubicin-doxorubicin dimer Acid-triggered release Mixed nanoparticles

limited Sea Surface Temperature Cooling Due to the Barrier Layer Promoting Super Typhoon Mangkhut(2018)

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Advances in Atmospheric Sciences 2024年第11期41卷 2156-2172页

作者： Huipeng WANG jiagen li Junqiang SONG liang SUN Fu liU Han ZHANG Kaijun REN Huizan WANG Chunming WANG Jinrong ZHANG Hongze LENG College of Meteorology and Oceanography National University of Defense TechnologyChangsha 410073China School of Earth and Space Sciences University of Science and Technology of ChinaHefei 230000China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519082China

This study investigates the impact of the salinity barrier layer(BL)on the upper ocean response to Super Typhoon Mangkhut(2018)in the western North *** the passage of Mangkhut,a noticeable increase(~0.6 psu)in sea surface salinity and a weak decrease(lightly warmed due to the entrainment of BL warm water,which suppressed the cooling induced by surface heat fluxes and horizontal *** the forced stage,SST cooling was controlled by entrainment,and the preexisting BL reduced the total cooling by 0.89℃ d-1,thus significantly weakening the overall SST cooling induced by *** the relaxation stage,the SST cooling was primarily caused by the *** results indicate that a preexisting BL can limit typhoon-induced SST cooling by suppressing the entrainment of cold thermocline water,which contributed to Mangkhut becoming the strongest typhoon in 2018.

关键词： sea surface cooling mixed-layer depth barrier layer typhoon

The combination of novel immune checkpoints HHLA2 and ICOSLG:A new system to predict survival and immune features in esophageal squamous cell carcinoma

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Genes & Diseases 2022年第2期9卷 415-428页

作者： Chaoqi Zhang Feng Wang Nan Sun Zhen Zhang Guochao Zhang Zhihui Zhang Yuejun Luo Yun Che Hong Cheng jiagen li Jie He Department of Thoracic Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021PR China Biotherapy Center The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan 450052PR China

Studies on immune checkpoint inhibitors targeting B7-CD28 family pathways in esophageal squamous cell carcinoma(ESCC)have shown promising ***,a comprehensive understanding of B7-CD28 family members in ESCC is still *** study aimed to construct a novel B7-CD28 family-based prognosis system to predict survival in patients with *** collected 179 cases from our previously published microarray data and 86 cases with qPCR ***,119 microarray data(GSE53624)were used as a training set,whereas the remaining 60 microarray data(GSE53622),all 179 microarray data(GSE53625)and an independent cohort with 86 qPCR data were used for *** underlying mechanism and immune landscape of the system were also explored using bioinformatics and *** examined 13 well-defined B7-CD28 family members and identified 2 genes(ICSOLG and HHLA2)with the greatest prognostic value.A system based on the combination HHLA2 and ICOSLG(B7-CD28 signature)was constructed to distinguish patients as high-or low-risk of an unfavorable outcome,which was further confirmed as an independent prognostic *** expected,the signature was well validated in the entire cohort and in the independent cohort,as well as in different clinical *** signature was found to be closely related to immune-specific biological processes and ***,high-risk group samples demonstrated high infiltration of Tregs and fibroblasts and distinctive immune checkpoint ***,we built the first,practical B7-CD28 signature for ESCC that could independently identify high-risk *** information may help inform immunotherapy-based treatment decisions for patients with ESCC.

关键词： Esophageal cancer HHLA2 ICOSLG Immune checkpoint Immunotherapy

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纳米Ti/PAM复合粒子的制备

功能材料 2015年第11期46卷 11148-11152页

作者：李梦李家艮朱健健中国民用航空飞行学院航空工程学院四川广汉618307

利用KH-560硅烷对纳米钛粉进行偶联处理,以十二烷基苯磺酸钠(SDBS)作为分散剂,通过原位聚合方法制备了纳米钛/聚丙烯酰胺(纳米Ti/PAM)复合粒子。运用XPS、TEM、FT-IR等测试手段对粉体材料进行表征,研究了偶联处理和原位聚合包覆对纳米... 详细信息

利用KH-560硅烷对纳米钛粉进行偶联处理,以十二烷基苯磺酸钠(SDBS)作为分散剂,通过原位聚合方法制备了纳米钛/聚丙烯酰胺(纳米Ti/PAM)复合粒子。运用XPS、TEM、FT-IR等测试手段对粉体材料进行表征,研究了偶联处理和原位聚合包覆对纳米钛粉在M03环氧树脂稀释液中分散能力的影响。结果表明,核壳结构的纳米Ti/PAM复合粒子粉体颗粒间不易团聚,将其分散在M03环氧树脂稀释液中之后混合液出现可目视观察的初始沉淀在20 h以上,且混合液中固体颗粒的分散状态较为稳定。

关键词：纳米钛粉偶联处理分散性原位聚合

Metal-to-ligand charge transfer chirality-based sensing of mercury ions

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Photonics Research 2021年第2期9卷 213-221页

作者： XIONGBIN WANG QIUSHI WANG YULONG CHEN jiagen li RUIKUN PAN XING CHENG KAR WEI NG XI ZHU TINGCHAO HE JIAJI CHENG ZIKANG TANG RUI CHEN Institute of Applied Physics and Materials Engineering University of MacaoAvenida da UniversidadeTaipaMacao 999078China Department of Electrical and Electronic Engineering Southern University of Science and TechnologyShenzhen 518055China School of Materials Science and Engineering Hubei UniversityWuhan 430062China Department of Materials Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Shenzhen Institute of Artificial Intelligence and Robotics for Society(AIRS) Shenzhen 518172China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China

Chiral ligand conjugated transition metal oxide nanoparticles(NPs) are a promising platform for chiral recognition, biochemical sensing, and chiroptics. Herein, we present chirality-based strategy for effective sensing of mercury ions via ligand-induced chirality derived from metal-to-ligand charge transfer(MLCT) effects. The ligand competition effect between molybdenum and heavy metal ions such as mercury is designated to be essential for MLCT chirality. With this know-how, mercury ions, which have a larger stability constant(Kf) than molybdenum, can be selectively identified and quantified with a limit of detection(LOD) of 0.08 and 0.12 nmol/L for D-cysteine and L-cysteine(Cys) capped Mo O2 NPs. Such chiral chemical sensing nanosystems would be an ideal prototype for biochemical sensing with a significant impact on the field of biosensing, biological systems,and water research-based nanotoxicology.

关键词： mercury molybdenum charge

长链非编码RNA在骨关节炎中的发病机制及中药干预

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中国组织工程研究 2024年第34期28卷 5571-5576页

作者：黄柯琪李加根陈尚桐容向宾广西中医药大学附属瑞康医院广西壮族自治区南宁市530011

背景:骨关节炎的病因多样且发病机制复杂,具体的发病机制至今仍未完全明确。随着生物信息领域近年的不断深入,越来越多研究发现可能是关节骨组织中长链非编码RNA(lncRNA)和微小核糖核酸(miRNA)异常表达介导下游相关信号通路参与骨关节... 详细信息

背景:骨关节炎的病因多样且发病机制复杂,具体的发病机制至今仍未完全明确。随着生物信息领域近年的不断深入,越来越多研究发现可能是关节骨组织中长链非编码RNA(lncRNA)和微小核糖核酸(miRNA)异常表达介导下游相关信号通路参与骨关节炎的发展。目的:综述lncRNA在骨关节炎发展过程中的作用机制,以及中药单体和复方有效成分调控lncRNA及下游信号通路对骨关节炎的治疗作用。方法:以“lncRNA,膝骨关节炎,miRNA,软骨细胞,信号通路,中药”为中文检索词检索中国知网、万方、维普数据库;以“long non-coding RNA,knee osteoarthritis,miRNA,chondrocytes,signal pathway,traditional chinese medicine”为英文检索词检索PubMed数据库,检索各数据库建库至2023年3月的相关文献,根据纳入及排除标准最终纳入61篇文献。结果与结论:(1)骨关节炎的发病机制涉及复杂的分子调控网络,其中包括软骨组织中lncRNA、miRNA的异常表达,这些异常表达可能导致软骨细胞凋亡、软骨细胞外基质降解及促炎细胞因子的大量生成,这些变化相互作用,导致关节软骨的退变和关节炎的进展,因此需要进一步深入的研究来揭示分子调控网络的精细机制;(2)中药治疗骨关节炎的作用机制主要集中在调节lncRNA、miRNA的表达进而减轻软骨细胞凋亡、细胞外基质降解及促进细胞增殖,延缓骨关节炎的发生发展。

关键词：骨关节炎 lncRNA ceRNA 中药治疗进展综述组织工程

基于动量理论的风力机三维工程全尾流模型研究

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太阳能学报 2024年第11期45卷 460-466页

作者：张萍刘洪威吴宁宇魏玉憧李佳根石建伟河北省电磁场与电器可靠性重点实验室(河北工业大学) 天津300130 河北工业大学人工智能与数据科学学院天津300130 河北建投海上风电有限公司唐山063000

为综合研究风力机尾流特性,将尾流区域分为近尾流区域和远尾流区域,并考虑风切变效应引起的风速差对尾流速度的影响,提出一种基于动量理论的三维工程全尾流模型(3DFM)。与风场实测数据对比,3DFM模型的近尾流区预测精度高达95.82%,远尾... 详细信息

为综合研究风力机尾流特性,将尾流区域分为近尾流区域和远尾流区域,并考虑风切变效应引起的风速差对尾流速度的影响,提出一种基于动量理论的三维工程全尾流模型(3DFM)。与风场实测数据对比,3DFM模型的近尾流区预测精度高达95.82%,远尾流区预测精度高达95.00%,验证了模型的有效性和准确性。根据来流风不同大气稳定状态修正尾流膨胀系数和风切变系数,详细研究大气稳定状态对尾流速度分布和恢复的影响。研究结果表明大气层越稳定,风切变系数越高,下游相同位置处的尾流区域面积越大,尾流速度恢复得越慢。

关键词：尾流效应风电场风力机全尾流风切变效应大气稳定性

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单孔胸腔镜肺叶袖状切除术治疗肺癌10例

中华胸心血管外科杂志 2019年第7期35卷 425-427页

作者：赵峻李嘉根曾庆鹏高树庚国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院胸外科 100021

近年来单孔胸腔镜手术发展迅速,但单孔胸腔镜肺叶袖状切除术因手术难度较高,目前仅少量个案报道。本研究总结10例单孔胸腔镜肺叶袖状切除术患者临床病理特征与手术经验。其中男9例,女1例;年龄(55.6±14.6)岁。手术切口取腋中线第4或5肋... 详细信息

近年来单孔胸腔镜手术发展迅速,但单孔胸腔镜肺叶袖状切除术因手术难度较高,目前仅少量个案报道。本研究总结10例单孔胸腔镜肺叶袖状切除术患者临床病理特征与手术经验。其中男9例,女1例;年龄(55.6±14.6)岁。手术切口取腋中线第4或5肋间,8例接受右肺上叶袖状切除术,1例右肺中下叶袖状切除术,1例左肺下叶袖状切除术。手术用时(238.0±71.7)min,1例中转开胸。术后4例出现并发症发,包括心律失常2例,肺炎1例,乳糜胸1例,均经对症治疗后好转。术后住院(7.2±2.6)天,无非计划二次手术,无围手术期死亡。本研究结果显示单孔胸腔镜肺叶袖状切除术可用于肺癌外科治疗,其安全性、疗效及优势还需进一步研究验证。

关键词：袖状切除术胸腔镜手术对症治疗单孔肺叶肺癌临床病理特征手术难度