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Redo pull-through in total colonic aganglionosis due to residual aganglionosis:a single center’s experience

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Gastroenterology Report 2021年第4期9卷 363-369,I0003页

作者： jia-Yu Yan Chun-Hui Peng Wen-Bo Pang Yong-Wei Chen Cai-Ling Ding Ya-jun Chen Department of General Surgery Beijing Children’s HospitalNational Center of Children’s HealthCapital Medical UniversityBeijingP.R.China Department of Neonatal Surgery Beijing Children’s HospitalNational Center of Children’s HealthCapital Medical UniversityBeijingP.R.China

Background Reoperation for total colonic aganglionosis(TCA)may be required for residual aganglionosis after an initial radical *** aimed to investigate the symptoms,management,and outcomes of patients who required a redo pullthrough(Redo PT).Methods Nine TCA patients underwent Redo PT at our center between 2007 and *** medical records were *** telephone interviews that included disease-specific clinical outcomes were conducted,and post-operative complications and long-termoutcomes(including height-for-age/weight-for-age and bowel-function score)were compared to those of single-pull-through(Single PT)patients(n=21).Results All the nine Redo PT patients suffered obstruction within 1 month after the initial operation that could not be alleviated by conservative *** abdominal X-ray/contrast barium enemas showed proximal bowel dilatation,indicating residual *** median ages at the initial operation and Redo PT were 200 and 509 days,*** consisted of an intraoperative frozen biopsy and a modified laparotomic Soave procedure in all *** complications included perianal excoriation(n=3),intestinal obstruction(n=2),enterocolitis(n=2),and rectovestibular fistula(n=1).Seven Redo PT patients were followed up for a mean time of 7.162.3 years;six(85.7%)had good growth and four(57.1%)had good bowel-function ***-operative complications and long-term outcomes were almost equal between the Redo PT and Single PT groups(all P>0.05).Conclusion TCA patients with recurrent obstructive symptoms and dilated proximal bowel may have residual aganglionosis after an initial *** PT is effective and provides good long-termoutcomes comparable to those of patients who benefited from Single PT.

关键词： total colonic aganglionosis residual aganglionosis redo pull-through

Position-sensitive plastic scintillator detector with WLS-fiber readout

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Nuclear Science and Techniques 2018年第8期29卷 186-192页

作者： jia-Ning Dong Yun-Long Zhang Zhi-Yong Zhang Dong Liu Zi-Zong xu Xiao-Lian Wang Shu-Bin Liu State Key Laboratory of Particle Detection and Electronics University of Science and Technology of China Department of Physics University of Science and Technology of China Department of Modern Physics University of Science and Technology of China

In this paper,the design,construction,simulation,and performance of a position-sensitive plastic scintillator detector are *** readout of the detector uses wavelength-shifting fibers coupled with multi-anode photomultipliers(PMTs) for the x-and *** calibrating the multi-anode PMTs,a two-dimensional projection image of the square scintillator telescope hit by cosmic muons is *** performing a cosmic test with the Micromegas telescope,the position resolution of the detector was determined to reach approximately8.6 mm,which is close to the value obtained in physical simulation.

关键词：闪烁器纤维结位置塑料敏感读出 WLS 光电倍增管

Effect of pre-culture on virus elimination from in vitro apple by thermotherapy coupled with shoot tip culture

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Journal of Integrative Agriculture 2018年第9期17卷 2015-2023页

作者： HU Guo-jun DONG Ya-feng ZHANG Zun-ping FAN xu-dong REN Fang LI Zheng-nan Research Institute of Pomology Chinese Academy of Agricultural Sciences

We evaluated the role of pre-culture on survival rate of in vitro apple plants treated by thermotherapy. Two apple cultivars, Malusxdomestica cv. Pink Lady and Huafu, were used in the experiment and both have widely grown in China and infected with Apple chlorotic leafspot virus （ACLSV） and Apple stem grooving virus （ASGV）. Results in growth and virus titer of apple plants did not exhibit clear trends during five different periods of pre-culture. Whilst, pre-culture increased the survival rate of the two cultivars during thermotherapy. The survival rate of plants pre-cultured for 13 d （P-13d） was 14 and 51% higher than that of P-ld plants for Pink Lady and Huafu, respectively. Moreover, pre-culture positively influenced regeneration of Huafu plants. The average survival rate of plants regenerated from P-ld and P-4d was 20% lower than that regenerated from P-7d, P-10d, and P-13d. The efficiency of virus eradication was determined by reverse-transcription PCR with two primer pairs for each virus, and the detection results showed that pre-culture scarcely affected apple virus elimination. Despite the fact that the two viruses were hardly detected at 5 d of thermotherapy, no virus-free plants were found in the two cultivars of regenerated apple plantlets after 30-d treatment.

关键词： apple in vitro culture pre-culture thermotherapy virus elimination

Metformin induces apoptosis of pancreatic cancer cells

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World Journal of Gastroenterology 2008年第47期14卷 7192-7198页

作者： Luo-Wei Wang Zhao-Shen Li Duo-Wu Zou Zhen-Dong Jin jun Gao Guo-Ming xu Department of Gastroenterology Changhai Hospital the Second Military Medical UniversityShanghai 200433 China

AIM:To assess the role and mechanism of metformin in inducing apoptosis of pancreatic cancer ***:The human pancreatic cancer cell lines ASPC-1,BxPc-3,PANC-1 and SW1990 were exposed to *** inhibition of cell proliferation and colony formation via apoptosis induction and S phase arrest in pancreatic cancer cell lines of metformin was ***:In each pancreatic cancer cell line tested,metformin inhibited cell proliferation in a dose dependent manner in MTS(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assays).Flow cytometric analysis showed that metformin reduced the number of cells in G1 and increased the percentage of cells in S phase as well as the apoptotic *** immunosorbent assay(ELISA) showed that metformin induced apoptosis in all pancreatic cancer cell *** Western blot studies,metformin induced poly-ADP-ribose polymerase(PARP) cleavage(an indicator of caspase activation) in all pancreatic cancer cell *** general caspase inhibitor(VAD-fmk) completely abolished metformin-induced PARP cleavage and apoptosis in ASPC-1 BxPc-3 and PANC-1,the caspase-8 specific inhibitor(IETD-fmk) and the caspase-9 specific inhibitor(LEHD-fmk) only partially abrogated metformin-induced apoptosis and PARP cleavage in BxPc-3 and PANC-1 *** also observed that metformin treatment dramatically reduced epidermal growth factor receptor(EGFR) and phosphorylated mitogen activated protein kinase(P-MAPK) in both a time-and dose-dependent manner in all cell lines ***:Metformin significantly inhibits cell proliferation and apoptosis in all pancreatic cell *** the metformin-induced apoptosis is associated with PARP cleavage,activation of caspase-3,-8,and-9 in a time-and dose-dependent ***,both caspase-8 and-9-initiated apoptotic signaling pathways contribute to metformin-induced apoptosis in pancreatic cell lines.

关键词： Metformin Pancreatic cancer Molecular classification Apoptosis

Novel linear search for support vector machine parameter selection

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Journal of Zhejiang University-Science C(Computers and Electronics) 2011年第11期12卷 885-896页

作者： Hong-xia PANG Wen-de DONG Zhi-hai xu Hua-jun FENG Qi LI Yue-ting CHEN State Key Laboratory of Optical Instrumentation Zhejiang University Hangzhou 310027 China

Selecting the optimal parameters for support vector machine (SVM) has long been a hot research topic. Aiming for support vector classification/regression (SVC/SVR) with the radial basis function (RBF) kernel, we summarize the rough line rule of the penalty parameter and kernel width, and propose a novel linear search method to obtain these two optimal parameters. We use a direct-setting method with thresholds to set the epsilon parameter of SVR. The proposed method directly locates the right search field, which greatly saves computing time and achieves a stable, high accuracy. The method is more competitive for both SVC and SVR. It is easy to use and feasible for a new data set without any adjustments, since it requires no parameters to set.

关键词： Support vector machine (SVM) Rough line rule Parameter selection Linear search Motion prediction

Identification and effective regulation of scarb1 gene involved in pigmentation change in autotetraploid Carassius auratus

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Zoological Research 2024年第2期45卷 381-397页

作者： Xi-Dan xu Yue Zhou Chong-Qing Wang xu Huang Kun Zhang Xiao-Wei xu Li-Wen He Xin-Yue Zhang Xin-Zhu Fu Ming Ma Qin-Bo Qin Shao-jun Liu State Key Laboratory of Developmental Biology of Freshwater Fish Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education MinistryCollege of Life SciencesHunan Normal UniversityChangshaHunan 410081China Nansha-South China Agricultural University Fishery Research Institute GuangzhouGuangdong 511458China Hunan Yuelu Mountain Science and Technology Co.Ltd.for Aquatic Breeding ChangshaHunan 410081China College of Chemistry and Chemical Engineering Hunan Normal UniversityChangshaHunan 410081China

The autotetraploid Carassius auratus(4nRR,4n=200,RRRR)is derived from whole-genome duplication of Carassius auratus red var.(RCC,2n=100,RR).In the current study,we demonstrated that chromatophores and pigment changes directly caused the coloration and variation of 4nRR skin(red in RCC,brownish-yellow in4nRR).To further explore the molecular mechanisms underlying coloration formation and variation in 4nRR,we performed transcriptome profiling and molecular functional verification in RCC and *** revealed that scarb1,associated with carotenoid metabolism,underwent significant down-regulation in *** editing of this candidate pigment gene provided clear evidence of its significant role in RCC ***,we identified four divergent scarb1 homeologs in 4nRR:two original scarb1 homeologs from RCC and two duplicated ***,three of these homeologs possessed two highly conserved alleles,exhibiting biased and allelespecific expression in the ***,after precise editing of both the original and duplicated scarb1homeologs and/or alleles,4nRR individuals,whether singly or multiply mutated,displayed a transition from brownishyellow skin to a cyan-gray ***,the proportional areas of the cyan-gray regions displayed a gene-dose *** findings illustrate the subfunctionalization of duplicated scarb1,with all scarb1genes synergistically and equally contributing to the pigmentation of *** is the first report concerning the functional differentiation of duplicated homeologs in an autopolyploidfish,substantiallyenrichingour understanding of coloration formation and change within this group of organisms.

关键词： Autopolyploidization Coloration change scarb1 Functional differentiation Genetic changes

Perioperative complications of radical retropubic prostatectomy in patients with locally advanced prostate cancer： a comparison with clinically localized prostate cancer

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Asian Journal of Andrology 2013年第2期15卷 241-245,I0007,I0008页

作者： xu-Dong Yao Xiao-jun Liu Shi-Lin Zhang Bo Dai Hai-Liang Zhang Ding-Wei Ye Department of Urology Cancer Hospital Fudan University Shanghai 200032 China Shanghai Medical College Fudan University Shanghai 200032 China

Radical prostatectomy （RP） continues to be an effective surgical therapy for prostate carcinoma, particularly for organ-confined prostate cancer （PCa）. Recently, RP has also been used in the treatment of locally advanced prostate cancer. However, little research has been performed to elucidate the perioperative complications associated with RP in patients with clinically localized or locally advanced PCa. We sought to analyse the incidence of complications in these two groups after radical retropubic prostatectomy （RRP）. From june 2002 to July 2010, we reviewed 379 PCa patients who underwent RRP in our hospital. Among these cases, 196 had clinically localized PCa （Tla-T2c group 1）, and 183 had locally advanced PCa （ ≥ T3,： group 2）. The overall complication incidence was 21.9%, which was lower than other studies have reported. Perioperative complications in patients with locally advanced PCa mirror those in patients with clinically localized PCa （26.2% vs. 17.8%, P=0.91）. Our results showed that perioperative complications could not be regarded as a factor to consider in regarding RP in patients with cT3 or greater.

关键词： complications clinically localized prostate cancer locally advanced prostate cancer prostate cancer (PCa) prostatectomy radical retropubic prostatectomy (RRP)

Three-dimensional artificial transpiration for efficient solar waste-water treatment

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National Science Review 2018年第1期5卷 70-77页

作者： Xiuqiang Li Renxing Lin George Ni Ning xu Xiaozhen Hu Bin Zhu Guangxin Lv Jinlei Li Shining Zhu jia Zhu National Laboratory of Solid State Microstructures College of Engineering and Applied SciencesSchool of Physics and Collaborative Innovation Center of Advanced MicrostructuresNanjing University Department of Mechanical Engineering Massachusetts Institute of Technology

Solar steam generation is emerging as promising solar-energy conversion technology for potential applications in desalination, sterilization and chemical purification. Despite the recent use of photon management and thermal insulation, achieving optimum solar steam efficiency requires simultaneous minimization of radiation, convection and conduction losses without compromising light *** by the natural transpiration process in plants, here we report a 3 D artificial transpiration device with all three components of heat loss and angular dependence of light absorption minimized, which enables over 85% solar steam efficiency under one sun without external optical or thermal management. It is also demonstrated that this artificial transpiration device can provide a complementary path for waste-water treatment with a minimal carbon footprint, recycling valuable heavy metals and producing purified water directly from waste water contaminated with heavy metal ions.

关键词： graphene solar vapor heatloss waste water heavy-metal ions water purification

Mutation inε-Sarcoglycan Induces a Myoclonus-Dystonia Syndrome-Like Movement Disorder in Mice

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Neuroscience Bulletin 2021年第3期37卷 311-322页

作者： jiao Li Yiqiong Liu Qin Li Xiaolin Huang Dingxi Zhou Hanjian xu Feng Zhao Xiaoxiao Mi Ruoxu Wang Fan jia Fuqiang xu Jing Yang Dong Liu xuliang Deng Yan Zhang State Key Laboratory of Membrane Biology College of Life SciencesPKU-IDG/McGovern Institute for Brain ResearchDepartment of Geriatric DentistryPeking University School and Hospital of StomatologyPeking UniversityBeijing 100871China Wuhan Institute of Physics and Mathematics Chinese Academy of SciencesWuhan 430071China College of Life Sciences Wuhan UniversityWuhan 430027China

Myoclonus dystonia syndrome(MDS)is an inherited movement disorder,and most MDS-related mutations have so far been found in theε-sarcoglycan(SGCE)coding *** generating SGCE-knockout(KO)and human 237 C>T mutation knock-in(KI)mice,we showed here that both KO and KI mice exerted typical movement defects similar to those of MDS *** promoted filopodia development in vitro and inhibited excitatory synapse formation both in vivo and in *** of function of SGCE leading to excessive excitatory synapses that may ultimately contribute to MDS ***,using a zebrafish MDS model,we found that among 1700 screened chemical compounds,Vigabatrin was the most potent in readily reversing MDS symptoms of mouse disease *** study strengthens the notion that mutations of SGCE lead to MDS and most likely,SGCE functions to brake synaptogenesis in the CNS.

关键词： SGCE MDS Filopodia Synapse Excitability