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Sucrose-responsive osmoregulation of plant cell size by a long non-coding RNA

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molecular Plant 2024年第11期17卷 1719-1732页

作者： Jakub Hajný Tereza Trávnícková martina pundová michelle Roenspies R.m.Imtiaz Karim Rony Sebastian Sacharowski michal Krzyszton David Zalabák Christian S.Hardtke Ales Pecinka Holger Puchta Szymon Swiezewski jaim.e m.van norman Ondrej Novák Laboratory of Growth Regulators Czech Academy of SciencesInstitute of Experimental Botany and Palacky UniversitySlechtitelu 2777900 OlomoucCzech Republic Department of Biophysics Faculty of SciencePalacky UniversitySlechtitelu 2777900 OlomoucCzech Republic Joseph Gottlieb Kölreuter Institute for Plant Sciences(JKIP)–molecular Biology Karlsruhe Institute of Technology76131 KarlsruheGermany Department of molecular Celland Systems BiologyUniversity of CaliforniaRiversideCA 92521USA Laboratory of Seeds molecular Biology Institute of Biochemistry and Biophysics Polish Academy of SciencesPawinskiego 5a02-106 WarsawPoland Department of Plant molecular Biology University of Lausanne1015 LausanneSwitzerland Center of Plant Structural and Functional Genomics Institute of Experimental BotanyCzech Academy of SciencesSlechtitelu 3177900 OlomoucCzech Republic Department of molecular Celland Developmental BiologyUniversity of CaliforniaLos AngelesLos AngelesCA 90095USA

In plants,sugars are the key source of energy and metabolic building *** systemic transport of sugars is essential for plant growth and *** evolved intricate molecular networks to effectively distribute *** dynamic distribution of these osmotically active compounds is a handy tool for regulating cell turgor pressure,an instructive force in developmental *** this study,we have investigated the molecular mechanism behind the dual role of the receptor-like kinase *** functionally characterized a long non-coding RNA,CARmA,as a negative regulator of *** CARmA specifically fine-tunes CANAR expression in the phloem,the route of sugar *** genetic,molecular,microscopy,and biophysical data suggest that the CARmA–CANAR module controls the shoot-to-root phloem transport of sugars,allows cells to flexibly adapt to the external osmolality by appropriate water uptake,and thus adjust the size of vascular cell types during organ growth and *** study identifies a nexus of plant vascular tissue formation with cell internal pressure monitoring,revealing a novel functional aspect of long non-coding RNAs in developmental biology.

关键词： lncRNA auxin turgor sugar transport cell size

Inelastic Cotunneling Current and Shot Noise of an Interacting Quantum Dot with Ferromagnetic Correlations

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Communications in Theoretical Physics 2007年第12期48卷 1099-1106页

作者： norman J.m.Horing Department of Physics and engineering Physics Stevens Institute of TechnologyHobokenNew Jersey 07030USA

We explore inelastic cotunneling through a strongly Coulomb-blockaded quantum dot attached to twoferromagnetic leads in the weak coupling limit using a generic quantum Langevin equation *** first developa B1och-type equation microscopically to describe the cotunneling-induced spin relaxation dynamics,and then developexplicit analytical expressions for the local magnetization,current,and its *** this basis,we predict a novelzero-bias anomaly of the differential conductance in the absence of a magnetic field for the anti-parallel configuration,and asymmetric peak splitting in a magnetic ***,for the same system with large polarization,we find a negativezero-frequency differential shot noise in the low positive bias-voltage region. All these effects are ascribed to rapidspin-reversal due to underlying spin-flip cotunneling.

关键词： quantum dot spin polarized tunneling cotunneling

Systolic and Diastolic Strain measurements Show Left Ventricular Dysfunction in Women with Turner Syndrome

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Congenital Heart Disease 2021年第4期16卷 357-368页

作者： Allard T.van den Hoven Sultan Yilmazer Lidia R.Bons Roderick W.Jvan Grootel L.W.Geenen An m.van Berendoncks Anthonie L.Duijnhouwer Hans-marc J.Siebelink Jolien W.Roos-Hesselink Annemien e.van den Bosch Department of Congenital Cardiology Erasmus MCRotterdamNetherlands Department of Cardiology Radboud University Medical CenterNijmegenNetherlands Department of Cardiology Leiden University Medical CenterRotterdamNetherlands

Aim:This study aimed to describe the systolic left ventricular global longitudinal strain(LvGLS)and left ventricular peak early diastolic strain rate(Sre)in adult women with Turner syndrome(TS)and to determine its relationship with exercise capacity and clinical ***:In this cross-sectional cohort study,consecutively included adult TS women underwent an electrocardiogram,transthoracic echocardiogram(TTe)and cardiopulmonary exercise test(CPeT)on the same *** and Sre were measured using 2D speckle tracking analysis(STe)and compared with age-matched healthy female ***:Ninety-four adult women(age 36±13 years)with TS and 32 healthy age-matched female controls were *** with TS had a significantly impaired/reduced systolic LvGLS(–17.82±2.98%vs.–21.80±1.85%,pe(0.98±0.32 s-1 vs.1.27±0.19 s-1,p>0.001),compared to healthy female ***,TS women had reduced diastolic function as measured by conventional echocardiographic parameters:A higher A-wave(per e/A-ratio(p=0.001),longer deceleration time(p=0.006),and a higher e/e’-ratio(pmen with TS also had a significantly reduced maximal workload(p=0.033),reduced oxygen uptake(peduced maximal heart rate(pear regression analysis revealed that Age,karyotype and QT-duration were significantly associated with Sre,but not with LvGLS,in the TS ***:Systolic and diastolic strain and exercise capacity were significantly reduced in TS women compared to healthy *** correlation between strain itself and exercise capacity could be demonstrated,but correlations with conventional TTe parameters and baseline characteristics were found.

关键词： Turnersyndrome speckletracking echocardiography strain exercise capacity

machine Learning in Chemical engineering:Strengths,Weaknesses,Opportunities,and Threats

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engineering 2021年第9期7卷 1201-1211页

作者： maarten R.Dobbelaere Pieter P.Plehiers Ruben van de Vijver Christian V.Stevens Kevin m.van Geem Laboratory for Chemical Technology Department of MaterialsTextiles and Chemical EngineeringGhent UniversityGhent 9052Belgium SynBioC Research Group Department of Green Chemistry and TechnologyFaculty of Bioscience EngineeringGhent UniversityGhent 9000Belgium

Chemical engineers rely on models for design,research,and daily decision-making,often with potentially large financial and safety *** efforts a few decades ago to combine artificial intelligence and chemical engineering for modeling were unable to fulfill the *** the last five years,the increasing availability of data and computational resources has led to a resurgence in machine learning-based *** recent efforts have facilitated the roll-out of machine learning techniques in the research field by developing large databases,benchmarks,and representations for chemical applications and new machine learning *** learning has significant advantages over traditional modeling techniques,including flexibility,accuracy,and execution *** strengths also come with weaknesses,such as the lack of interpretability of these black-box *** greatest opportunities involve using machine learning in time-limited applications such as real-time optimization and planning that require high accuracy and that can build on models with a self-learning ability to recognize patterns,learn from data,and become more intelligent over *** greatest threat in artificial intelligence research today is inappropriate use because most chemical engineers have had limited training in computer science and data ***,machine learning will definitely become a trustworthy element in the modeling toolbox of chemical engineers.

关键词： Artificial intelligence machine learning Reaction engineering Process engineering

COmPOSITION AND RHeOLOGY OF POLYAmIDe-6 OBTAINeD BY USING BI-AND TRI-FUNCTIONAL COUPLING AGeNTS

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Chinese Journal of Polymer Science 2013年第1期31卷 58-70页

作者： J.J.m. Slot e. van Ruymbeke P.A.m. Steeman materials Science Centre DSM Research Department of mathematics and Computer Science Eindhoven University of Technology

A statistical approach is developed, based on a monte Carlo method, in order to determine the statistical composition of a polyamide-6 sample composed of caprolactam （an AB-type monomer） and of a di-acid （A2 type） or a tri- acid （A3 type） as coupling agents. For this composition, the linear theological behavior of these systems is predicted using a tube-based theory. This allows us to show that while coupling agents of type A2 can be seen as flow improver, the effect of branching agents of type A3, depending on the synthesis recipe and the conversion level, can lead either to an increase or to a decrease of the viscosity. By adding specific amount of these agents, we also show that it is possible to obtain materials with the same zero-shear viscosity but with different shear thinning behavior. Furthermore, the polydispersity of linear samples of the same average number molecular weight, mn, is discussed in function of the amount of A2 monomers they contain. Ranging from 2 to 1.5, this difference in polydispersity is expected to have a significant influence on the processing behavior of such materials.

关键词： Polyamide-6 Random branching Statistical composition Rheology monte Carlo Tube theory.

The sexually dimorphic expression of glutamate transporters and their implication in pain after spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3317-3329页

作者： Jennifer m.Colón-mercado Aranza I.Torrado-Tapias Iris K.Salgado Jose m.Santiago Samuel e.Ocasio Rivera Dina P.Bracho-Rincon Luis H.Pagan Rivera Jorge D.miranda Department of Physiology University of Puerto RicoMedical Sciences CampusSan JuanPRUSA National Institutes of Health National Human Genome Research InstituteBethesdaMDUSA Universidad Central del Caribe School of MedicineBayamónPRUSA University of Puerto Rico Carolina CampusCarolinaPRUSA Institute of Neurobiology San JuanPRUSA

In addition to the loss of motor function,~60% of patients develop pain after spinal cord *** cellular-molecular mechanisms are not well understood,but the data suggests that plasticity within the rostral,epicenter,and caudal penumbra of the injury site initiates a cellularmolecular interplay that acts as a rewiring mechanism leading to central neuropathic *** can lead to the formation of new connections triggering abnormal sensory *** excitatory glutamate transporters are responsible for the reuptake of extracellular glutamate which makes them a critical target to prevent neuronal hyperexcitability and *** previous studies showed a sexually dimorphic therapeutic window for spinal cord injury after treatment with the selective estrogen receptor modulator *** this study,we investigated the anti-allodynic effects of tamoxifen in male and female rats with spinal cord *** hypothesized that tamoxifen exerts anti-allodynic effects by increasing the expression of glutamate transporters,leading to reduced hyperexcitability of the secondary neuron or by decreasing aberrant *** and female rats received a moderate contusion to the thoracic spinal cord followed by subcutaneous slow-release treatment of tamoxifen or matrix pellets as a control(placebo).We used von Frey monofilaments and the“up-down method”to evaluate mechanical *** treatment decreased allodynia only in female rats with spinal cord injury revealing a sexdependent *** expression profile of glutamatergic transporters(excitatory amino acid transporter 1/glutamate aspartate transporter and excitatory amino acid transporter 2/glutamate transporter-1)revealed a sexual dimorphism in the rostral,epicenter,and caudal areas of the spinal cord with a pattern of expression primarily on *** rodents showed a significantly higher level of excitatory amino acid transporter-1 expression while male rodents showed increased

关键词： allodynia central neuropathic pain eAAT-1/GLAST eAAT-2/GLT-1 glutamate transporters selective estrogen receptor modulator sexual dimorphism spinal cord injury trauma

Increased Drying Rate Lowers the Critical Water Content for Survival in embryonic Axes of english Oak (Quercus robur L.) Seeds

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Journal of Integrative Plant Biology 2011年第4期53卷 270-280页

作者： Tobias m.Ntuli William e.Finch-Savage Patricia Berjak norman W.Pammenter Plant Germplasm Conservation Research School of Biological and Conservation SciencesUniversity of KwaZulu-NatalWestville CampusPrivate Bag X54001Durban 4000South Africa Department of Life and Consumer Sciences University of South AfricaPrivate Bag X6Florida 1710JohannesburgSouth Africa Warwick HRI Warwick UniversityWellesbourneWarwick CV35 9EFUK Plant Germplasm Conservation Research School of Biological and Conservation SciencesUniversity of KwaZulu-NatalWestville

The potential to cryopreserve embryonic axes of desiccation-sensitive（recalcitrant） seeds is limited by damage during the desiccation necessary for low temperature survival,but the basis of this injury and how to reduce it is not well *** effects of drying rate on the viability,respiratory metabolism and free radical-mediated processes were therefore investigated during dehydration of Quercus robur L embryonic ***,assessed by evidence of germination and tetrazolium staining,showed a sharp decline at 0.27 and 0.8 g/g during rapid（〈12 h） or slow（3d） dehydration,*** dehydration therefore lowered the critical water content for *** any given water content rapid dehydration was associated with higher activities of the free radical processing enzymes,superoxide dismutase,catalase and glutathione reductase and lower levels of hydroperoxide and membrane *** dehydration was also associated with lower malate dehydrogenase activity,and a reduced decline in phosphofructokinase activity and in levels of the oxidized form of nicotinamide *** may have contributed to increased damage during slow dehydration,since viability declined even in hydrated storage after 3 *** results presented are consistent with rapid dehydration reducing the accumulation of damage resulting from desiccation induced aqueous-based deleterious reactions.

关键词： Quercus robur L Seeds Figure NADH

Competition for shelter between four invasive gobiids and two native benthic fish species

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Current Zoology 2011年第6期57卷 844-851页

作者： N. van KeSSeL m. DOReNBOSCH m.R.m. De BOeR R.S.e.W. LeUVeN G. van DeR VeLDe Radboud University Nijmegen Institute for Water and Wetland Research Department of Animal Ecology and Ecophysiology EO. Box 9010 6500 GL Nijmegen The Netherlands Radboud University Nijmegen Institute for Water and Wetland Research Department of Environmental Science P.O. Box 9010 6500 GL Nijmegen The Netherlands Natuurbalans - Limes Divergens P.O. Box 31070 6503 CB Nijmegen The Netherlands Netherlands Institute of ecology （NIOO - KNAW） EO. Box 50 6700 AB Wageningen The Netherlands Netherlands Centre for Biodiversity Naturalis EO. Box 9517 2300 RA Leiden The Netherlands

Recent invasions by non-native gobiid fish species that are ongoing in the Western european rivers Rhine and meuse, will lead to interactions with native benthic fish species. Since both non-native gobiids and native benthic species are bottom dwelling species with a preference for shelter during at least part of their life cycle, it is likely that competition for shelter will occur between these non-native and native species when shelter is a limiting factor. To investigate the importance of this mecha- nism for species replacements, various habitat choice experiments were conducted between two common native benthic fish spe- cies （Cottus perifretum and Barbatula barbatula） and four invasive non-native gobiid species （Proterorhinus semilunaris, Neogo- bius melanostomus, N. kessleri and N. fluviatilis）. The first series of single specimen experiments determined the habitat choice of each individual fish species. In a second series of competition experiments, shifts in habitat choice in comparison with the previ- ously observed habitat choice, were determined when a native benthic fish species co-occurred with non-native gobiid species. Native C. perifretum displayed a significant shift in habitat choice in co-occurrence with the gobiids N. kessleri or P. semilunaris. C. perifretum was outcompeted and moved from the available shelter place to less preferred habitat types. During the competition experiments no change in habitat choice of B. barbatula was shown. Our study therefore suggests that competition for shelter is likely to occur in rivers invaded by N. kessleri and P. semilunaris at sites where shelter is limiting

关键词： Bullhead exotic species Habitat choice Invasive gobies Rivers Rhine and meuse Stone loach

Skin tribology:Science friction?

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Friction 2013年第2期1卷 130-142页

作者： e.van DeR HeIDe X.ZeNG m.A.mASeN Laboratory for Surface Technology and Tribology Faculty of Engineering TechnologyUniversity of TwenteDrienerlolaan 57522 NB EnschedeThe Netherlands TNO De Rondom 15612 AP EindhovenThe Netherlands

The application of tribological knowledge is not just restricted to optimizing mechanical and chemical engineering *** fact,effective solutions to friction and wear related questions can be found in our everyday *** important part is related to skin tribology,as the human skin is frequently one of the interacting surfaces in relative *** seem to solve these problems related to skin friction based upon a trial-and-error strategy and based upon on our sense for *** question of course rises whether or not a trained tribologist would make different choices based upon a science based strategy?In other words:Is skin friction part of the larger knowledge base that has been generated during the last decades by tribology research groups and which could be referred to as Science Friction?This paper discusses the specific nature of tribological systems that include the human skin and argues that the living nature of skin limits the use of conventional *** tribology requires in vivo,subject and anatomical location specific test *** predictive friction models can only partially be applied to predict in vivo skin *** reason for this is found in limited understanding of the contact mechanics at the asperity level of product-skin interactions.A recently developed model gives the building blocks for enhanced understanding of friction at the micro *** largely simplified power law based equations are currently available as general engineering ***,the need for friction control is illustrated by elaborating on the role of skin friction on discomfort and *** texturing and polymer brush coatings are promising directions as they provide way and means to tailor friction in sliding contacts without the need of major changes to the product.

关键词： friction bio-tribology skin soft tissue surface texture brush coatings