Increased Drying Rate Lowers the Critical Water Content for Survival in Embryonic Axes of English Oak (Quercus robur L.) Seeds

作     者：Tobias M.Ntuli William E.Finch-Savage Patricia Berjak Norman W.Pammenter 

作者机构：Plant Germplasm Conservation ResearchSchool of Biological and Conservation SciencesUniversity of KwaZulu-NatalWestville CampusPrivate Bag X54001Durban 4000South Africa Department of Life and Consumer SciencesUniversity of South AfricaPrivate Bag X6Florida 1710JohannesburgSouth Africa Warwick HRIWarwick UniversityWellesbourneWarwick CV35 9EFUK Plant Germplasm Conservation ResearchSchool of Biological and Conservation SciencesUniversity of KwaZulu-NatalWestville 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2011年第53卷第4期

页      面：270-280页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：AcknowledgementsWe thank M. T. Smith for advice and the National Research Foundation of South Africa for financial assistance 

主　　题：Quercus robur L Seeds Figure NADH 

摘      要：The potential to cryopreserve embryonic axes of desiccation-sensitive（recalcitrant） seeds is limited by damage during the desiccation necessary for low temperature survival,but the basis of this injury and how to reduce it is not well *** effects of drying rate on the viability,respiratory metabolism and free radical-mediated processes were therefore investigated during dehydration of Quercus robur L embryonic ***,assessed by evidence of germination and tetrazolium staining,showed a sharp decline at 0.27 and 0.8 g/g during rapid（〈12 h） or slow（3d） dehydration,*** dehydration therefore lowered the critical water content for *** any given water content rapid dehydration was associated with higher activities of the free radical processing enzymes,superoxide dismutase,catalase and glutathione reductase and lower levels of hydroperoxide and membrane *** dehydration was also associated with lower malate dehydrogenase activity,and a reduced decline in phosphofructokinase activity and in levels of the oxidized form of nicotinamide *** may have contributed to increased damage during slow dehydration,since viability declined even in hydrated storage after 3 *** results presented are consistent with rapid dehydration reducing the accumulation of damage resulting from desiccation induced aqueous-based deleterious reactions.