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Journal of Oceanology and Limnology 2024年第2期42卷 664-675页

作者： Xin PENG Haihui TU Xinyi YAO Xuan LAN Zhenxiao ZHONG jinping luo Qiongying TANG Shaokui YI Zhenglong XIA Guoliang YANG Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development College of Life SciencesHuzhou UniversityHuzhou 313000China Jiangsu Shufeng Prawn Breeding Co. Ltd.Gaoyou 225654China

To identify the cause of mass mortality of adult Macrobrachium rosenbergii in a farm in Gaoyou City,Jiangsu Province,China,a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying *** and identified as Aeromonas dhakensis by purification culture,biochemical characterization,and 16S rRNA and gyrB gene sequence *** results of the challenge test revealed that the strain was highly pathogenic and the 50%lethal dose(LD_(50))in 72 h to *** was 1.54×10^(5)CFU/*** amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes,including ascV,aexT,aer,act,lip,ompAI,gcaT,acg,and exu,supporting the strong virulence of strain DKQ-1 to *** observation of the hepatopancreas,gills,and intestines indicated that DKQ-1 injection into *** could cause serious tissue damage,which further supported the strong virulence of this *** addition,a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics,resistant to 9 kinds of antibiotics,and had intermediate resistance to spectinomycin and *** study is the first report of *** isolated from *** and provided a reference for the pathogen identification of bacterial diseases in ***,and for the prevention and treatment caused by ***.

关键词： Aeromonas dhakensis histopathology virulence gene gyrB drug susceptibility test Macrobrachium rosenbergii

Simulation and fabrication of in vitro microfluidic microelectrode array chip for patterned culture and electrophysiological detection of neurons

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Nanotechnology and Precision Engineering 2024年第2期7卷 1-10页

作者： Yan Yang Shihong Xu Yu Deng Yaoyao Liu Kui Zhang Shiya Lv Longze Sha Qi Xu Xinxia Cai jinping luo State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural *** chip was designed based on the specific structure of neurons and the requirements for detection and ***-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic *** employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated *** hippocampal neurons were cultured on the chip,forming a well-defined neural *** electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode *** study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.

关键词： Microfluidic Microelectrode array In vitro Neural chip

Isolation,identification,and evaluation of intestinal bacteria in Macrobrachium rosenbergii

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Journal of Oceanology and Limnology 2024年第5期42卷 1710-1721页

作者： Xiuxin ZHAO jinping luo Peimin LIU Hao HUANG Zhenheng CHENG Xin PENG Qiongying TANG Guoliang YANG Shaokui YI Quanxin GAO Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development Key Laboratory of Aquatic Animal Genetic Breeding and NutritionChinese Academy of Fishery SciencesCollege of Life ScienceHuzhou UniversityHuzhou 313000China Jiangsu Shufeng Prawn Breeding Co.Ltd. Gaoyou 225654China

Probiotics are live microbial food supplements that have been shown to have beneficial effects on animal *** probiotic bacteria have long been used for their proposed health promoting properties and have become a hot research topic in growth improvement in *** endogenous probiotic bacteria from intestines of Macrobrachium rosenbergii(giant river prawn)was explored for their probiotic potential,from which 367 bacterial strains were isolated from the intestine of *** 16 S rDNA sequence analysis,234 isolates were identified as Lactococcus garvieae,which accounted for 63.76%of the total number of culturable intestinal bacteria,suggesting that this bacterium was the main component of the ***,to reveal the probiotic properties of ***,this isolated bacterial strain was characterized morphologically,physiologically,and *** enzyme production capacity,bacteriostatic activity,and resistance to acid,high temperature,and pH,were *** vitro experiments showed that the ***(No.C 6 a 2)had a fast growth rate and entered the logarithmic phase ***,it had characteristics of acid-production and resistance,enzyme-producing capacity,and strong antibacterial activity against pathogenic Staphylococc us aureus,Aeromonas hydrophila,and Aeromonas ***,it lacked the ability to tolerate high *** results provide novel data to deepen our understanding of the intestinal bacteria structure of *** and valuable information for probiotic screening and the application for ***.

关键词： Macrobrachium rosenbergii intestinal bacteria probiotic Lactococcus garvieae

顿范畴中的相对Gorenstein投射对象

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山东大学学报（理学版） 2024年第2期59卷 100-104,119页

作者：罗金萍梁力兰州交通大学数理学院甘肃兰州730070

给定Abel范畴A和B,以及右正合函子F:A→B,依据A和B中的相对Gorenstein投射对象,给出了顿范畴(F,B)中相对Gorenstein投射对象的等价刻画。

关键词：顿范畴相对Gorenstein投射对象 Abel范畴

Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay

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Chinese Journal of Electronics 2017年第1期26卷 152-155页

作者： LIU Juntao QU Shuxue luo jinping WANG Bin CAI Xinxia State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences University of Chinese Academy of Sciences

Carcinoembryonic antigen(CEA) is one of the most widely used tumor markers worldwide. A portable system was designed for rapid field specific test of CEA based on immunomagnetic separation and chemiluminescence immunoassay, which consisted of chemiluminescent immunosensor and optical test instrument. The chemiluminescent immunosensor was based on immunomagnetic separation and chemiluminescence immunoassay, which utilized a immuno-sandwich scheme as-say with Horseradish peroxidase(HRP) labeled anti-CEA antibody and Immunomagnetic beads(IMBs). And the test instrument consisted of optical detection module and central processing and control system. The optical noise of this instrument was below 40 photons/s. The system could achieve a sample test in 30 minutes, and the detecting result showed a good liner relationship between the intensity of luminescence and concentration of CEA in the range from 1 to1000ng/m L, with a correlation coefficient of 0.9858. Real human samples were also detected with this system in comparison with clinical results and a correlation coefficient of0.9934 was obtained. The instrument is portable and easy to operate, which will provide potential application in cancer clinical test.

关键词： Chemiluminescence immunoassay Carcinoembryonic antigen Rapid field test Optical sensor FPGA NIOS II processor

A NEW QUANTITATIVE DETECTION METHOD OF RECOMBINANT CFP10-ESAT6 AMALGAMATION PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS BASED ON MICRO-MAGNETIC PROBES STRATEGY

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Journal of Innovative Optical Health Sciences 2012年第1期5卷 55-60页

作者： YIQING HUANG jinping luo MIXIA WANG JUNTAO LIU XINXIA CAI State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100190China Graduate School of Chinese Academy of Sciences Beijing 100190 China

A new rapid,specific and sensitive method for assay of recombinant CFP10-ESAT6 amalgamation proteins from Mycobacterium tuberculosis was *** method used streptavidincoated magnetic beads to enrich the specific biotinylated anti-CFP10 antibody,then adopted a sandwich-type enzyme linked immunosorbent assay technology with two kinds of monoclonal antibodies:biotinylated anti-CFP10 antibody and HRP-labeled anti-CFP10 antibody to identify the target CFP10-ESAT6 proteins,and finally detected chemiluminescence intensity by a small home-made optical *** was shown that,the corresponding chemiluminescence intensity had a good logarithmic linear response to the concentration of CFP10-ESAT6 proteins when ranging at 1~1000 ng/mL,and the correlation coefficient is *** proposed method could detect the CFP10-ESAT6 proteins with low detection limit(1 ng/mL)and the detection time could be controlled within 45 *** with commonly used detection methods of ***,this method was easy to operate,faster,and of higher *** achievement of the quantitative detection of CFP10-ESAT6 proteins has important scientific significance and wide application prospects in tuberculosis control.

关键词： Enzyme linked immunosorbent assay chemiluminescence home-made optical sensor

基于三维分析框架的我国家庭医生签约服务政策量化研究

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中国全科医学 2024年第25期27卷 3100-3107页

作者：孙嘉颖罗金萍张倩雯王康尹文强陈钟鸣马东平山东第二医科大学管理学院山东省潍坊市261053 “健康山东”重大社会风险预测与治理协同创新中心山东省潍坊市261053 上海市健康相关重大社会风险预警协同创新中心 200032 山东省潍坊市人民医院财务部 261000

背景我国家庭医生签约服务工作全面推进不足7年,仍面临着一系列发展问题,保障机制不健全是阻碍家庭医生签约服务发展的关键问题,亟须科学且合理的家庭医生签约服务政策来保障工作的有效开展。目的对我国家庭医生签约服务政策的文本内容... 详细信息

背景我国家庭医生签约服务工作全面推进不足7年,仍面临着一系列发展问题,保障机制不健全是阻碍家庭医生签约服务发展的关键问题,亟须科学且合理的家庭医生签约服务政策来保障工作的有效开展。目的对我国家庭医生签约服务政策的文本内容进行量化分析,探究现有政策的侧重点及不足,为后续家庭医生签约服务政策的发展和优化提供依据与参考。方法于2023-01-10,检索中国政府、国家卫生健康委员会等官方网站,获取与家庭医生签约服务相关的政策文件15份,检索时限为2015—2022年。构建政策工具-利益相关者-政策力度三维分析框架,对政策文件进行分类、编码及分析。结果政策工具维度中供给型、需求型、环境型工具分别占30.5%(69/226)、19.0%(43/226)、50.5%(114/226),利益相关者维度中家庭医生、签约者、未签约者、基层医疗卫生机构、医院(二级及以上)、政府分别占29.2%(123/422)、14.7%(62/422)、9.2%(39/422)、21.3%(90/422)、13.3%(56/422)、12.3%(52/422),我国家庭医生签约服务政策的平均力度值为2.2分。政策工具-利益相关者交叉维度中,利益相关者在供给型、环境型工具中的分布情况欠佳,子工具存在缺位现象;政策工具-政策力度交叉维度中,环境型工具随着政策力度的加大受到更多使用;利益相关者-政策力度交叉维度中,各利益相关者的政策力度匹配分数具有较大差异,家庭医生主体分数最高(311分),未签约者主体分数最低(90分)。结论政策工具角度,应合理配置政策工具,不断调整使用比重,优化内部结构,并提升利益相关者间政策工具分布的合理性;利益相关者角度,应统筹兼顾所有利益相关者,明确各自定位,着重关注未签约者需求;政策力度角度,应强化政策监督管理能力,持续提高家庭医生签约服务政策的执行力度。

关键词：家庭医生签约服务政策工具利益相关者政策力度政策分析

Microelectrode arrays for monitoring neural activity in neural stem cells with modulation by glutamate in vitro

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Nanotechnology and Precision Engineering 2020年第2期3卷 69-74页

作者： Fei Gao jinping luo Yilin Song Enhui He Yu Zhang Guihua Xiao Xinxia Cai State Key Laboratory of Transducer Technology Institute of ElectronicsChinese Academy of SciencesBeijing 100190PR China University of Chinese Academy of Sciences Beijing 100049PR China Institute of Electronics Chinese Academy of Sciences

In this study, a 60-channel microelectrode array(MEA) was fabricated and used to monitor the neural spikes and local field potentials(LFPs) of neurons differentiated from rat neural stem cells in vitro. The neurons were grown on the MEA surface to detect neural signals. Glutamate(Glu) was used to modulate neural activity during experiments. To enhance detection performance, platinum nanoparticles were modified onto the microelectrode site surface. Glutamate stimulated neural spikes and LFPs were recorded using the MEA. The average spike amplitude was approximately 70 μV in the normal state. The spike amplitude increased by 29% from 70 μV to 90 μV with Glu modulation. The firing rate increased by 69% from 4.01 Hz to 6.8 Hz with Glu modulation. The LFP power increased from 326 μW in the normal state to 617 μW with Glu modulation in the 0–10 Hz frequency band. Data analysis shows that neural activity stimulated by Glu modulation was recorded experimentally at high temporal-spatial resolution. These results may provide a new neuron detection method, as well as further understanding of neural stem cell spike firing and associated mechanisms.

关键词： MEA Glutamate Spikes Neural stem cells In vitro

Rapid detection of Cyfra 21-1 by optical-biosensing based on chemiluminescence immunoassay using bio-functionlized magnetic nanocomposites

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Chinese Science Bulletin 2013年第21期58卷 2567-2569页

作者： luo jinping QU ShuXue LIU JunTao WANG Bin CAI XinXia State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Graduate School of Chinese Academy of Sciences

This letter reports a chemiluminescene immunoassay method combined with immunomagnetic separation to rapidly detect Cyfra 21-1, in which bio-functionlized magnetic nanocomposites were used as mobile substrate for capturing and isolating the cyfra 21-1 proteins. After the captured Cyfra 21-1 further reacted with horseradish peroxidase-conjugated anti-Cyfra 21-1 antibody to form a sandwich immunocomplex, the chemiluminescence would be produced as a result of addition of the chemiluminescent substrate. A home-made optical biosensor was designed to detect the chemiluminescence instead of other large instruments. There is a good linear response between the chemiluminescence intensity and the concentration of Cyfra 21-1 in the range from 0.2 to 50 ng/mL. The whole detection process including incubation, washing and detection could be performed within 45 min. The proposed method offers a simple, noninvasive and reliable tool for detecting non-small cell lung cancer and has potential application for clinical testing.

关键词：化学发光免疫分析纳米复合材料光学传感器快速检测生物传感磁性辣根过氧化物酶非小细胞肺癌

An implantable microelectrode array for simultaneous L-glutamate and electrophysiological recordings in vivo

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Microsystems & Nanoengineering 2015年第1期1卷 159-164页

作者： Wenjing Wei Yilin Song Li Wang Song Zhang jinping luo Shengwei Xu Xinxia Cai State Key Laboratory of Transducer Technology Institute of ElectronicsChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

L-glutamate,the most common excitatory neurotransmitter in the mammalian central nervous system(CNS),is associated with a wide range of neurological *** neurons in CNS communicate with each other both electrically and chemically,dualmode(electric and chemical)analytical techniques with high spatiotemporal resolution are required to better understand glutamate function in *** the present study,a silicon-based implantable microelectrode array(MEA)composed of both platinum electrochemical and electrophysiological microelectrodes was fabricated using micro-electromechanical *** the MEA probe,the electrophysiological electrodes have a low impedance of 0.018 MΩat 1 kHz,and the electrochemical electrodes show a sensitivity of 56 pAμM^(−1) to glutamate and have a detection limit of 0.5μ*** MEA probe was used to monitor extracellular glutamate levels,spikes and local field potentials(LFPs)in the striatum of anaesthetised *** explore the potential of the MEA probe,the rats were administered to KCl via intraperitoneal injection.K+significantly increases extracellular glutamate levels,LFP low-beta range(12–18 Hz)power and spike firing rates with a similar temporal profile,indicating that the MEA probe is capable of detecting dual-mode neuronal *** was concluded that the MEA probe can help reveal mechanisms of neural physiology and pathology in vivo.

关键词： MEMS implantable microelectrode array glutamate electrophysiological detection in vivo