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Isomorphism-based robust right coprime factorization for uncertain nonlinear feedback systems

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Science China(Information Sciences) 2020年第10期63卷 261-263页

作者： longguo jin Ni BU Mingcong DENG Haier School of Qingdao Technical College College of Automation and Electronic Engineering Qingdao University of Science and Technology Department of Electrical and Electronic Engineering Tokyo University of Agriculture and Technology

Dear editor,In system engineering,most controlled systems exhibit nonlinear dynamics [1,2],where the exact mathematical model cannot be derived owing to uncertainties such as ***,the performance of the concerned syste... 详细信息

关键词： Isomorphic operator Robustness Right coprime factorization Uncertainty Nonlinear system

MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

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Chinese Science Bulletin 2012年第28期57卷 3804-3810页

作者： LUO ZhongQin jin longguo QIU LiJuan The National Key Faeility for Crop Gene Resources and Genetic Improvement(NFCRI) Institute of Crop ScienceChinese Academy of Agricultural SeiencesBeijing 100081China

MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5'RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.

关键词：靶基因切割 miRNA Northern 大豆 blot分析小RNA RACE

Expression profiles and transactivation analysis of a novel ethylene-responsive transcription factor gene GhERF5 from cotton

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Progress in Natural Science:Materials International 2009年第5期19卷 563-572页

作者： longguo jin, Bo Huang, Hui Li, jinyuan Liu Laboratory of Molecular Biology and MOE Laboratory of Protein Science, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China [a]Laboratory of Molecular Biology and MOE Laboratory of Protein Science Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Plant AP2/EREBP transcription factors play important roles in plant development and in plant responses to biotic and abiotic stresses. A novel gene for ethylene-responsive element binding protein (EREBP), designated GhERF5, which encodes a protein of 255 amino acids, was isolated by RACE-PCR from cotton (Gossypium hirsutum) seedlings. Sequence alignment revealed that GhERF5 contains a typical AP2/ERF domain, and belongs to the B3 subgroup of the ERF subfamily. Particle bombardment assay showed that GhERF5 functions as an in vivo transcription activator in tobacco cells, and it is located in the nuclei of onion epidermis cells. Semi-quantitative RT-PCR revealed that the expression of GhERF5 was highly and rapidly induced when plants were treated with exogenous ethylene, abscisic acid (ABA), salt, cold and drought. Promoter analysis indicated that there are conserved cis-acting elements induced by these stresses in the 50-upstream region of the GhERF5 gene. These results suggest that the GhERF5 gene might play an important role in cotton response to ethylene, ABA and environmental stresses.

关键词： Ethylene-responsive element binding factor (ERF) Abiotic stresses Nuclear localization Transactivation Gossypium hirsutum

Molecular cloning and functional characterization of a DREB1/CBF-like gene (GhDREB1L) from cotton

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Science China(Life Sciences) 2007年第1期50卷 7-14页

作者： HUANG Bo, jin longguo & LIU jinYuan Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China 1. Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

The transcription factors DREB1s/CBFs play important roles in the regulation of plant resistance to environmental stresses and are quite useful for generating transgenic plants tolerant to these stresses. In the present work, a cDNA encoding DREB1/CBF-like protein (GhDREB1L) from cotton was isolated, and its sequence features, DNA binding preference, and expression patterns of the transcripts were also characterized. GhDREB1L contained one conserved AP2/ERF domain and its amino acid sequence was similar to the DREB1/CBF group of the DREB family from other plants. The DNA-binding domain of GhDREB1L was successfully expressed as a fusion protein in Escherichia coli BL21 (DE3) and purified by Ni-NTA affinity chromatography. Electrophoretic mobility shift assay revealed that the purified GhDREB1L fusion protein had a specific binding activity with the previously characterized DRE ele-ment (core sequence, ACCGAC) and also with the DRE-like sequence (core sequence, GCCGAC) in the promoter of the dehydration-responsive late embryogenesis-abundant gene LEA D113. Semi-quantita- tive RT-PCR showed that GhDREB1L was induced in the cotton cotyledons by low temperature, as well as drought and NaCl treatments. These results suggested that the novel cotton GhDREB1L might play an important role in response to low temperature as well as drought and high salinity through binding to the DRE cis-element.

关键词： cotton DREB1/CBF cold dehydration LEA D113

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MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

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Science Bulletin 2012年第Z2期 3804-3810页

作者： LUO ZhongQin,jin longguo & QIU LiJuan * The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China

MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5’RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.

关键词： soybean miR1511 miR1511 * GmRPL4a

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基于PLC的智能温室控制系统设计

电气传动自动化 2022年第3期44卷 27-31页

作者：金龙国青岛职业技术学院

随着国民经济高速发展，人民生活水平不断提高，传统的农业生产模式难以适应人们对高质量生活的追求，传统的温室大棚只能起到恒温作用，不能自动化监测和控制光照、温度、土壤湿度、CO2浓度等温室环境因素。智能温室大棚控制系统通过... 详细信息

随着国民经济高速发展，人民生活水平不断提高，传统的农业生产模式难以适应人们对高质量生活的追求，传统的温室大棚只能起到恒温作用，不能自动化监测和控制光照、温度、土壤湿度、CO2浓度等温室环境因素。智能温室大棚控制系统通过温室大棚与信息技术相结合，借助温室大棚的气候效应与隔离效果实现智能、自动化农业生产，以此实现旱涝保收、空间隔绝、反季节调控等作用，因此，基于PLC技术的温室大棚控制系统具有良好的应用推广价值。本文采用S7-200PLC模块，结合土壤湿度传感器、温度传感器、光照传感器、CO2浓度传感器和执行机构，可实现温室环境参数自动化控制，实现温室大棚环境因素自动化补偿，弥补传统大棚环境因素监测和控制不足的问题。

关键词： PLC 温室大棚控制系统系统设计

Development and utilization of a new chemically-induced soybean library with a high mutation density

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Journal of Integrative Plant Biology 2017年第1期59卷 60-74页

作者： Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu jinhui Lei Ying Liu Rongxia Guan Yong Guo longguo jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural Science Soybean Research Institute Heilongjiang Academy of Agricultural Sciences College of Agriculture Yangtze University

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate（EMS）-induced soybean（Glycine max） population,consisting of 21,600 independent M_2 lines, was *** 1,000 M_（4（5））families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll（Chl） content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene（ChlI1a） results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

关键词： soybean mutant yellow chemically golden phenotype genomics mutation Glycine chloroplast

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Shor's algorithm does not factor large integers in the presence of noise

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Science China(Information Sciences) 2024年第7期67卷 305-320页

作者： jin-Yi CAI College of Letters and Science University of Wisconsin

We consider Shor's quantum factoring algorithm in the setting of noisy quantum gates. Under a generic model of random noise for(controlled) rotation gates, we prove that the algorithm does not factor integers of the form pq when the noise exceeds a vanishingly small level in terms of n—the number of bits of the integer to be factored, where p and q are from a well-defined set of primes of positive density. We further prove that with probability 1-o(1) over random prime pairs(p, q), Shor's factoring algorithm does not factor numbers of the form pq, with the same level of random noise present.

关键词： random noise Shor’s algorithm rotation gates quantum computing prime factorization

Semi-analytical Solutions for Wellbores with Graded Skin Zones in Poroelastic Media

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Acta Mechanica Solida Sinica 2024年第3期37卷 385-395页

作者： Zhiqiang Fan Zhihe jin School of Mechanics Civil Engineering and ArchitectureNorthwestern Polytechnical UniversityXi’an710072China Department of Mechanical Engineering University of MaineOronoME04469USA

In analyzing the complex interaction between the wellbore and the reservoir formation,the hydromechanical properties of the region proximal to the wellbore,referred to as the“wellbore skin zone”,play a pivotal role in determining flow dynamics and the resulting formation *** models of the wellbore skin zone generally assume a constant permeability throughout,resulting in a sharp permeability discontinuity at the skin-reservoir *** paper introduces a model for a wellbore with a continuously graded skin zone of finite thickness within a poroelastic *** solutions are derived using the Laplace transform method,addressing both positive and negative skin *** results are presented to illustrate the effects of graded permeability/skin zone thickness on pore pressures and stresses around a *** results highlight a distinct divergence in stress and pore pressure fields when comparing wellbores with negative skin zones to those with positive skin zones or no skin at all.

关键词： Poroelastic Graded permeability Analytical solution Wellbore Skin zone