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Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers

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Acta Oceanologica Sinica 2007年第1期26卷 122-129页

作者： wang Shifeng DU jiaying wang Jun DING Shaoxiong College of Oceanography and Environment Science Xiamen University Xiamen 361005 China

Using multi-molecular marker technologies and based on morphological criteria, the genetic relationship between Epinepheelus malabaricus and E, coioides was examined in the hope of resolving the long-standing issue of identifying these two species. Results showed that：（ 1 ） E. coioides and E. malabaricus should be identified as two species, the consistency of mitochondrial DNA cytochrome b gene sequence between E. malabaricus and E. coioides is 94.4%, and the genetic similarity by AFLP was 0.753 9 ; （2） Hybridization exists between E. malabaricus and E. coioides, the specific RAPD and AFLP fragments are found to be useful in the identification of these two species, and the genetic properties （both with exterior and inheritance） of hybrid is significantly biased to the male parents ; and （3） AFLP was a potentially powerful tool in constructing the genetic linkage map for these two groupers.

关键词： Epinephelus malabaricus Epinephelus coioides hybridize molecular identification

Physicochemical factors affecting the wettability of copper mine blasting dust

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International Journal of Coal Science & Technology 2021年第2期8卷 265-273页

作者： Longzhe Jin Jianguo Liu Jingzhong Guo jiaying wang Tianyang wang School of Civil and Resource Engineering University of Science and Technology BeijingBeijing 100083China Key Laboratory of High-Efficient Mining and Safety of Metal Mines of the Ministry of Education University of Science and Technology BeijingBeijing 100083China Mine Emergency Technology Research Center University of Science and Technology BeijingBeijing 100083China College of Safety Engineering North China Institute of Science and TechnologySanhe 101601China

To investigate the factors affecting the wettability of copper mine blasting dust,the primary blasting dust was collected from an open-pit copper mine and separated into hydrophilic blasting dust(HLBD)and hydrophobic blasting dust(HBBD)using water flotation *** physicochemical properties of HLBD and HBBD were measured and compared with each *** properties included particle size distributions(PSDs),micromorphologies,pore structures,mineral components and surface organic carbon functional *** results show that particle size and pore structure of the blasting dust are the main factors affecting its ***,particle size of HBBD is smaller than that of HLBD,and their respiratory dust(less than 10μm)accounts for 61.74 vol%and 53.00 vol%,*** pore structure of HBBD is more developed,and the total pore volume of HBBD is 1.66 times larger than that of *** identical mineral compositions were detected in HLBD and HBBD by X-rays diffraction(XRD);however,the surface organic hydrophobic component of HBBD is slightly larger than that of HLBD,this may be the reason for the poor wettability of *** study is significant to understand the effects of physicochemical properties of copper mine blasting dust on its wettability.

关键词： Blasting dust Copper mine Wettability Physicochemical properties

Cooperative coupling of photocatalytic production of H_(2)O_(2) and oxidation of organic pollutants over gadolinium ion doped WO_(3) nanocomposite

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Chinese Chemical Letters 2023年第9期34卷 258-263页

作者： jiaying wang Jiejie wang Sijin Zuo Jianchuan Pei Weiping Liu Juan wang Institute of Environmental Health MOE Key Laboratory of Environmental Remediation and Ecosystem HealthCollege of Environment and Resources ScienceZhejiang UniversityHangzhou 310058China College of Environment and Resources Zhejiang A&F UniversityHangzhou 311300China

This work reported the lanthanide ion(Gd^(3+))doped tungsten trioxide(Gd-WO_(3))nanocrystal for remarkable promoted photocatalytic degradation of organic pollutants and simultaneous in-situ H_(2)O_(2)*** doped lanthanide ion(Gd^(3+)),Gd-WO_(3)showed a much broad and enhanced solar light absorption,which not only promoted the photocatalytic degradation efficiency of organic compounds,but also provided a suitable bandgap for direct reduction of oxygen to H_(2)O_(2).Additionally,the isolated Gd^(3+)on WO_(3)surface can efficiently weaken the*OOH binding energy,increasing the activity and selectivity of direct reduction of oxygen to H_(2)O_(2),with a rate of 0.58 mmol L^(-1)g^(-1)h^(-1).The in-situ generated H_(2)O_(2)can be subsequently converted to·OH based on Fenton reaction,further contributed to the overall removal of organic *** results demonstrate a cascade photocatalytic oxidation-Fenton reaction which can efficiently utilize photo-generated electrons and holes for organic pollutants treatment.

关键词： Lanthanide ion Photocatalytic oxidation Photocatalytic generation of H_(2)O_(2)in situ Cascade reaction

Microstructure and mechanical properties of SiC_(f)/SiBCN ceramic matrix composites

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Journal of Advanced Ceramics 2015年第1期4卷 31-38页

作者： jiaying wang Zhihua YANG Xiaoming DUAN Dechang JIA Yu ZHOU Institute for Advanced Ceramics Harbin Institute of TechnologyHarbin 150080Chin

SiC fiber reinforced SiBCN ceramic matrix composites(CMCs)have been prepared by mechanical alloying and consolidated by hot *** the sintering process,amorphous SiC fibers crystallized seriously and transformed ***,the interfacial carbothermal reactions caused the strong bonding between the matrix and *** a result,SiCf/SiBCN fractured in a typical catastrophic ***-temperature mechanical properties reached the maximums for the CMC samples sintered at 1900℃/60 MPa/30 *** density,flexural strength,Young’s modulus and fracture toughness are 2.56±0.02 g/cm^(3),284.3±17.9 MPa,183.5±11.1 GPa and 2.78±0.14 MPa·m^(1/2),respectively.

关键词： hot pressing composites mechanical properties fiber

Tobacco Rattle Virus-induced Phytoene Desaturase (PDS) Silencing in Centaurea cyanus

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Horticultural Plant Journal 2021年第2期7卷 159-166页

作者： Chengyan Deng Fan Zhang jiaying wang Yanfei Li He Huang Silan Dai Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding National Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological Environment and College of Landscape ArchitectureBeijing Advanced Innovation Center for Tree Breeding by Molecular DesignBeijing Forestry UniversityBeijing 100083China

Virus-induced gene silencing(VIGS)is a genetic tool used to assess gene *** rattle virus(TRV)is a VIGS vector commonly used to induce endogenous gene silencing in ***,there is no VIGS system established for Centaurea *** evaluated the effectiveness of a TRV-based VIGS system using phytoene desaturase(PDS)as a reporter gene in Centaurea *** methods including pressure-,vacuum-and apical meristem-infiltrationwere tested to infect *** *** leaveswere only obtained using apicalmeristem-infiltration after a 14 d *** CcPDS transcripts in photobleached leaves were significantly reduced compared with that in green leaves treated with empty *** *** cultivars were tested to detect their VIGS responses,and‘Dwarf Tom Pouce Blue’was the most *** agro-infiltration condition was optimized by screening for the optimal seedling stage as well as the optimum Agrobacterium density for efficient *** with four true leaves and infiltration with an Agrobacterium density of OD_(600)0.5 were optimal conditions to obtain more photobleached leaves and more intense photobleached *** results demonstrated the feasibility of TRV-based VIGS for functional analysis of genes in ***.

关键词： Centaurea cyanus VIGS tobacco rattle virus PDS seedling photobleached

Semi-interpenetrating polymer networks toward sulfonated poly(ether ether ketone) membranes for high concentration direct methanol fuel cell

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Chinese Chemical Letters 2019年第2期30卷 299-304页

作者： Xupo Liu Yunfeng Zhang Shaofeng Deng Cuicui Li Jiaming Dong jiaying wang Zehui Yang Deli wang Hansong Cheng Sustainable Energy Laboratory Faculty of Materials Science and Chemistry China University of Geosciences (Wuhan) Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology) Ministry of Education Hubei Key Laboratory of Material Chemistry and Service Failure School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Low methanol permeability of proton exchange membranes (PEMs) is greatly important for direct methanol fuel cells (DMFCs). Here, sulfonated poly (ether ether ketone) (SPEEK) based semiinterpenetrating polymer networks (semi-IPNs) are successfully prepared by interpenetrating SPEEK into the in-situ synthesized crosslinking networks. The polymeric networks are formed by the covalent bonds between bromobenzyl groups of bro mo methylated poly (phenylene oxide) and amine groups of diamine linkers as well as the ionic bonds between amine species and sulfonated groups. Two linkers without and with sulfonated groups are applied to fabricate the semi-IPNs. The core properties of the membranes, like phase separation, water uptake, proton conductivity and methanol permeability, are systematically studied and compared. The DMFCs assembled by using the semi-IPN membranes display better performance than Nafion 117 in high concentration methanol solutions. The present work provides a facile way to prepare PEMs with enhanced DMFC performance.

关键词： Direct methanol fuel cell Proton exchange membrane Semi-interpenetrating polymer networks Sulfonated poly(ether ether ketone) Bromomethylated poly(phenylene oxide)

The loss of function of HEL,which encodes a cellulose synthase interactive protein,causes helical and vine-like growth of tomato

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Horticulture Research 2020年第1期7卷 493-502页

作者： Qihong Yang Xiaoshuai Wan jiaying wang Yuyang Zhang Junhong Zhang Taotao wang Changxian Yang Zhibiao Ye Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural UniversityWuhan 430070China

Helical growth is an economical way for plant to obtain *** classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell ***,the molecular mechanism underlying tomato helical growth remains ***,we identified a spontaneous tomato helical(hel)mutant with right-handed helical cotyledons and petals but left-handed helical stems and true *** analysis revealed that the hel phenotype was controlled by a single recessive *** map-based cloning,we cloned the HEL gene,which encodes a cellulose interacting protein homologous to CSI1 of *** identified a 27 bp fragment replacement that generated a premature stop *** experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type *** contrast,the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical *** findings shed light on the molecular control of the helical growth of tomato.

关键词： helical cellulose walls

Flavonoid extracts from chrysanthemum with appropriate anthocyanins turn blue when exposed to iron ions

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Horticultural Plant Journal 2024年第3期10卷 837-852页

作者： Yanfei Li jiaying wang Chenfei Lu Zhongman wang Chengyan Deng Kang Gao Jingjing Li Zhijun Fang Hao Liu Yan Hong Silan Dai Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding National Engineering Research Center for FloricultureBeijingLaboratory of Urban and Rural Ecological EnvironmentKey Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of EducationSchool of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China Beijing Greenland Maintenance and Management Service Center Beijing 102211China Beijing North Asia Landscape Co. Beijing 102211China

Although some species that accumulate only cyanidin(Cy)in nature can produce blue flowers through iron ions,there has been no evidence of blue chrysanthemums being generated in this *** study revealed that flavonoid extracts from the ray florets of the chrysanthemum cultivar‘Wandai Fengguang’turned blue when exposed to Fe^(3+).Samples that could turn blue were labeled as CB(Cy-determined blue flowers),while samples that did not turn blue were labeled as CN(Cy-determined non-blue flowers).After a series of experiments,a stable screening system was established using flavonoid extracts containing NaAc buffer at pH 5.5 and a total anthocyanin concentration(TAC)of 30 μmol·L^(-1),and the addition of Fe^(3+)from 0 to 0.25 μmol·L^(-1)allowed for the selection of five CB samples from 39 chrysanthemum *** five CB samples exhibited flower color phenotypes that belonged to Cluster-I with redness(a*)values ranging from 29.03 to 45.99,yellowness(b*)values from-11.31 to 3.77,and brightness(L*)values from 29.07 to ***,the ratio of TAC to total luteolin concentration(TLC)was found to be a critical factor for distinguishing between CB and CN *** realize the desired blue hue in the flavonoid extracts with the participation of Fe^(3+),a TAC to TLC ratio of 2.25 and above is ***,the protoplasts and ray florets of CB samples that turned blue with the involvement of Fe^(2+)showed great potential for cultivating blue chrysanthemums through ferric-anthocyanin ***,this study reveals that blue flowers can be cultivated through the increase in the iron ion concentration,combined with the accumulation of Cy.

关键词： Chrysanthemum×morifolium Cyanidin Copigment Ferric-anthocyanin chelate Blue flower

Research Progress of Public Participation in National Park Affairs in Foreign Countries Based on CiteSpace

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Journal of Landscape Research 2020年第2期12卷 119-122页

作者： wang jiaying School of Public Management Tianjin University of CommerceTianjin 300134China

It is of great value and practical significance to study the theoretical system and practice mode of public participation in national park *** the basis of foreign literatures on public participation in national parks collected in Web of Science database,CiteSpace is used to elaborate from four aspects:general situation,main contents and research comments of public participation in national parks (protected areas),and implications for China *** results showed that the studies on public participation in national parks in foreign countries started early,with wide range of fields and multi-disciplinary characteristics,but the studies also had *** author considers that China should systematically improve the research in related fields.

关键词： Public participation National parks Protected area CiteSpace