The loss of function of HEL,which encodes a cellulose synthase interactive protein,causes helical and vine-like growth of tomato

作     者：Qihong Yang Xiaoshuai Wan Jiaying Wang Yuyang Zhang Junhong Zhang Taotao Wang Changxian Yang Zhibiao Ye Qihong Yang;Xiaoshuai Wan;Jiaying Wang;Yuyang Zhang;Junhong Zhang;Taotao Wang;Changxian Yang;Zhibiao Ye

作者机构：Key Laboratory of Horticultural Plant BiologyMinistry of EducationHuazhong Agricultural UniversityWuhan 430070China 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2020年第7卷第1期

页      面：493-502页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by grants from the NSFC(31672149,31991182 and 31872122) the National Key R&D Program of China(2017YFD0101902) 

主　　题：helical cellulose walls 

摘      要：Helical growth is an economical way for plant to obtain *** classic microtubule–microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell ***,the molecular mechanism underlying tomato helical growth remains ***,we identified a spontaneous tomato helical(hel)mutant with right-handed helical cotyledons and petals but left-handed helical stems and true *** analysis revealed that the hel phenotype was controlled by a single recessive *** map-based cloning,we cloned the HEL gene,which encodes a cellulose interacting protein homologous to CSI1 of *** identified a 27 bp fragment replacement that generated a premature stop *** experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type *** contrast,the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical *** findings shed light on the molecular control of the helical growth of tomato.