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Ellagic acid induces apoptosis through inhibition of nuclear factor κB in pancreatic cancer cells

World Journal of Gastroenterology 2008年第23期14卷 3672-3680页

作者： Mouad Edderkaoui Irina Odinokova izumi ohno Ilya Gukovsky Vay Liang W Go Stephen J Pandol Anna S Gukovskaya Department of Medicine Veterans Affairs Greater Los Angeles Healthcare System and University of CaliforniaLos Angeles CA 90073CaliforniaUnited States Institute of Theoretical and Experimental Biophysics Russian Academy of SciencesPushchinoMoscow Region 142290Russia

AIM： To determine the effect of ellagic acid on apoptosis and proliferation in pancreatic cancer cells and to determine the mechanism of the pro-survival effects of ellagic acid.METHODS： The effect of ellagic acid on apoptosis was assessed by measuring Phosphatidylserine externalization, caspase activity, mitochondrial membrane potential and DNA fragmentation; and proliferation by measuring DNA thymidine incorporation. Mitochondrial membrane potential was measured in permeabilized cells, and in isolated mitochondria. Nuclear factor κB （NF-κB） activity was measured by electromobility shift assay （EMSA）. RESULTS： We show that ellagic acid, a polyphenolic compound in fruits and berries, at concentrations 10 to 50 mmol/L stimulates apoptosis in human pancreatic adenocarcinoma cells. Further, ellagic acid decreases proliferation by up to 20-fold at 50 mmol/L. Ellagic acid stimulates the mitochondrial pathway of apoptosis associated with mitochondrial depolarization, cytochrome C release, and the downstream caspase activation. Ellagic acid does not directly affect mitochondria. Ellagic acid dose-dependently decreased NF-κB binding activity. Furthermore, inhibition of NF-κB activity using IkB wild type plasmid prevented the effect of ellagic acid on apoptosis. CONCLUSION： Our data indicate that ellagic acid stimulates apoptosis through inhibition of the prosurvival transcription factor NF-κB.

关键词： Ellagic acid Nuclear factor-κB Apoptosis Pancreatic cancer

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Current status of diagnosis and therapy for intraductal papillary neoplasm of the bile duct

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World Journal of Gastroenterology 2021年第15期27卷 1569-1577页

作者： Yuji Sakai Masayuki Ohtsuka Harutoshi Sugiyama Rintaro Mikata Shin Yasui izumi ohno Yotaro Iino Jun Kato Toshio Tsuyuguchi Naoya Kato Department of Gastroenterology and Nephrology Graduate School of MedicineChiba UniversityChiba 260-8670Japan Department of General Surgery Graduate School of MedicineChiba UniversityChiba 260-8670Japan

Bile duct epithelial tumours showing papillary neoplasm in the bile duct lumen are present in the intrahepatic and extrahepatic bile *** images of these tumours are distinctive and diverse,including histological images with a low to high grade dysplasia,infiltrating and noninfiltrating characteristics,excessive mucus production,and similarity to intraductal papillary mucinous neoplasm(IPMN)of the *** World Health Organization Classification of Tumours of the Digestive System in 2010 named these features,intraductal papillary neoplasm of the bile duct(IPNB),as precancerous lesion of biliary *** is currently classified into type 1 that is similar to IPMN,and type 2 that is not similar to *** of IPNB spreads superficially,and diagnosis with cholangioscopy is considered mandatory to identify accurate localization and *** of IPNB is said to be better than normal bile duct cancer.

关键词： Intraductal papillary neoplasm of the bile duct Intraductal papillary mucinous neoplasm of the pancreas Peroral cholangioscopy Cholangioscopy

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Remission of primary low-grade gastric lymphomas of the mucosa-associated lymphoid tissue type in immunocompromised pediatric patients

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World Journal of Gastroenterology 2006年第16期12卷 2625-2628页

作者： Yasuharu ohno Taichirou Kosaka izumi Muraoka Takashi Kanematsu Akira Tsuru Eiichi Kinoshita Hiroyuki Moriuchi Division of Pediatric Surgery Department of SurgeryNagasaki University Graduate School of Biomedical SciencesNagasakiJapan Department of Pediatrics Nagasaki University Graduate School of Biomedical SciencesNagasakiJapan

We report the remission of primary gastric lymphoma of the mucosa-associated lymphoid tissue （MALT） type in two immunocompromised pediatric patients. Patient 1, a 14-year-old boy in an immunocompromised state of unknown cause, complained of repeated abdominal pain. Examinations revealed gastric MALT with local invasion and lymph node involvement. Serum anti-Helicobacter pylori （H pylon） antibody was positive. H pylori eradication was abandoned due to its adverse effects. The MALT lesion spontaneously regressed over the next 24 months without any treatment for lymphoma. Patient 2, a 6-year-old boy, underwent cord blood transplantation for the treatment of adrenoleukodystrophy. He was administered immunosuppressants for graft-versus-host disease after transplantation. Nausea and hematochezia appeared and further examinations revealed gastric MALT with H pylori gastritis. Treatment consisting of medication for the Hpylori infection alone eradicated the Hpylori and completely resolved the patient＇s MALT lesion, as well. Patients i and 2 were followed up over periods of 10 years and 3 years, respectively, without any signs of relapse. In conclusion, gastric lymphoma of the MALT type can be cured by conservative treatment even in immunocompromised pediatric patients.

关键词： Pediatric gastric lymphoma IVlucosa-associated lymphoid tissue Immunocompromised states

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Factors that affect the process of professional identity formation in public health nurses

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Open Journal of Nursing 2013年第1期3卷 8-15页

作者： Mika Okura Miyoko Uza Hisako izumi Masami ohno Hidenori Arai Kazuko Saeki Department of Human Health Sciences Kyoto University Graduate School of Medicine Kyoto Japan Department of Site Management Organization Sehma Co. Ltd. Fukui Japan Faculty of Health Sciences Hokkaido University Sapporo Japan School of Health Sciences Faculty of Medicine University of the Ryukyus Nishihara Japan School of Health Sciences Sapporo Medical University Sapporo Japan

Objective: The aim of this study was to elucidate the process of professional identity formation and the factors that affect the process in public health nurses (PHNs). Methods: We performed an inductive qualitative analysis based on semi-structured interviews and focus group interviews of 65 PHNs. Results: At years 1 - 5, PHNs did not have a professional self-identity. Therefore, it was important for them to recognize the significance of their own work. At years 6 - 10, PHNs acquired self-assurance as a practitioner through feedback from residents. It was important for them to have a better understanding, broader viewpoints and more affection for their own community. At years 11 - 20, PHNs felt ambivalent toward the challenges and heavy responsibilities based on the objective assessment of their jobs and roles. PHNs felt embarrassed by role changes when they became a manager or an expert. It was important for them to become aware of their new role through managerial education and training. However, some PHNs who were unable to establish a clear PHN identity experienced setbacks at each developmental stage. Conclusions: Our data suggest that a differential approach dependent on the stage and level of confidence in an individual’s PHN identity is required for the formation of PHN identity.

关键词： Professional Identity Public Health Nurse Professionalism Career Development

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Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E

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World Journal of Gastroenterology 2005年第41期11卷 6410-6415页

作者： Kei Fujiwara Yasuhito Tanaka Etsuro Orito Tomoyoshi ohno Takanobu Kato Kanji Sugihara izumi Hasegawa Mayumi Sakurai Kiyoaki Ito Atsushi Ozasa Yuko Sakamoto Isao Arita Ahmed El-Gohary Agossou Benoit Sophie I Ogoundele-Akplogan Namiko Yoshihara Ryuzo Ueda Masashi Mizokami Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences NagoyaJapan Department of Clinical Molecular Informative Medicine Nagoya City University Graduate School of Medical Sciences NagoyaJapan AIDS Research Center National Institute of Infectious Disease TokyoJapan Agency for Cooperation in International Health Kumamoto Japan Clinical Pathology Department Faculty of Medicine Suez Canal UniversityIsmailiaEgypt Central National Blood Transfusion Center Cotonou Benin Hematology Lab-CNHUC Cotonou Benin

AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant ***: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct ***: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/*** comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/***: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E.

关键词： HBV genotype West Africa Basic corepromoter

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