Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E

作     者：Kei Fujiwara Yasuhito Tanaka Etsuro Orito Tomoyoshi Ohno Takanobu Kato Kanji Sugihara Izumi Hasegawa Mayumi Sakurai Kiyoaki Ito Atsushi Ozasa Yuko Sakamoto Isao Arita Ahmed El-Gohary Agossou Benoit Sophie I Ogoundele-Akplogan Namiko Yoshihara Ryuzo Ueda Masashi Mizokami 

作者机构：Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical SciencesNagoyaJapan Department of Clinical Molecular Informative Medicine Nagoya City University Graduate School of Medical SciencesNagoyaJapan AIDS Research Center National Institute of Infectious DiseaseTokyoJapan Agency for Cooperation in International Health KumamotoJapan Clinical Pathology Department Faculty of MedicineSuez Canal UniversityIsmailiaEgypt Central National Blood Transfusion Center CotonouBenin Hematology Lab-CNHUC CotonouBenin 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2005年第11卷第41期

页      面：6410-6415页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

主　　题：HBV genotype West Africa Basic corepromoter 

摘      要：AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant ***: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct ***: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/*** comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic divers ity (nucleotide homology 96.7-99.2%).Based on the sequences in the basic core promoter (BCP)to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/***: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics,is observed in HBV/E.