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Evaluation of antimicrobial activity of a lectin isolated and purified from <i>Indigofera heterantha</i>

Advances in Bioscience and Biotechnology 2013年第11期4卷 999-1006页

作者： Sakeena Qadir ishfak hussain wani Shaista Rafiq Showkat Ahmad Ganie Akbar Masood Rabia Hamid Department of Biochemistry University of Kashmir Srinagar India

Indigofera heterantha commonly called indigo bush is a member of leguminoseae family found in the Himalayan region of Kashmir. A lectin has been isolated from the seeds of Indigofera heterantha by the purification procedure involving anion exchange chromatography on DEAE-cellulose followed by gel filtration chromatography on Sephadex G 100. Molecular characterization of the lectin was done by gel filtration and SDS-PAGE. Activity of the lectin was checked by hemagglutination assay and the sugar specificity by sugar inhibition tests. The antimicrobial activity of the purified lectin was carried out by Agar disc diffusion using appropriate standards. On the ion exchange column, the bound protein when eluted with 0-0.5 M NaCl gradient emerged as three peaks—peak I, peak II and peak III out of which only peak II showed the hemagglutinating activity. The lectin further resolved into two peaks G1 and G2 on gel filtration, with the lectin activity residing in G1, corresponding to a molecular weight of 70 KDa. The purified lectin named as Indigofera heterantha Lectin (IHL) produced a single band on SDS PAGE (18 KDa), revealing the tetrameric nature of the lectin. It agglutinated human erythrocytes (A, B, AB, and O). Hemagglutination was inhibited by D-galactose, Dmannose and D-arabinose. The lectin is reasonably thermostable showing full activity within a temperature range of 30°C to 90°C. pH stability of the lectin falls in the range of 2-9. IHL demonstrated a remarkable antibacterial activity against the pathogenic bacteria Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. IHL also inhibited the growth of phytopathogenic fungi Aspergillus niger, Aspergillus oryzae and Fusarium oxysporum.

关键词： Indigofera heterantha Lectin Hemagglutination Antimicrobial

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Impact of liver metastasis on immunotherapy in gastric carcinoma

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World Journal of Gastrointestinal Surgery 2024年第10期16卷 3084-3086页

作者： Mushtaq Chalkoo Mohd Yaqoob Bhat Yaser hussain wani Department of Surgery Government Medical CollegeSrinagar 190014Jammu and KashmirIndia Department of General Surgery Government Medical College SrinagarSrinagar 190010Jammu and KashmirIndia Post Graduate Department of Minimal Access and General Surgery Government Medical College SrinagarSrinagar 190010Jammu and KashmīrIndia

The editorial discusses the impact of liver metastasis on immunotherapy efficacy in gastric cancer(GC)*** metastasis can hinder the effectiveness of immunotherapy by altering the immune microenvironment,leading to systemic loss of T-cells and reduced treatment *** suggest that liver meta-stases serve as a negative baseline factor for immunotherapy efficacy,resulting in poorer progression-free survival and objective response *** such as liver-mediated radiotherapy may help improve treatment outcomes by reshaping the liver’s immune microenvironment and reducing T-cell ***-ing the complex interplay between liver metastasis and immunotherapy response is crucial for optimising patient care in GC.

关键词： Immunotherapy Liver metastasis Immune tolerance Gastric carcinoma Hepatic Siphoning

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Development and characterization of SSR markers in Himalayan species Betula utilis

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Journal of Forestry Research 2020年第4期31卷 1453-1460页

作者： Mohammad Saleem wani Vikas Sharma Raghbir Chand Gupta Abid hussain Munshi Department of Botany Punjabi University PatialaPatialaPunjabIndia Department of Botany University of KashmirSrinagarJammu&KashmirIndia Department of Botany Sant Baba Bhag Singh UniversityKhialaJalandharPunjabIndia

Betula utilis *** an important species of alpine Himalaya and forms the major treeline component of western *** different populations of *** are declining and are under high *** the present study,novel expressed sequence tag-simple sequence repeat(EST-SSR)primers were developed from expressed sequence tag(EST)data of different Betula *** the10,796 designed primers,the percentages of di-,tri-,tetra-,penta-,and hexa-repeats were 36%,35%,15%,5.5%and7.7%,*** validation,50 primers were synthesized randomly and were characterized in 20 different *** accessions from north-western *** these,45 primers amplified fragments in a range of *** 24 polymorphic primers produced 111 fragments in aggregate with 4.6 fragments on *** information content(PIC)ranged from 0.288 in marker BUMS-24 to 0.497 in BUMS-3 and BUMS-7,with an average of 0.447 among polymorphic *** based on Jaccard’s similarity coefficient and UPGMA method showed that newly developed SSR markers distinguished twenty accessions of *** into two *** no SSR markers were available in this species,the newly developed markers will foster molecular genetics research and conservation efforts for this species.

关键词： Betula utilis Simple sequence repeat(SSR) Western Himalaya Polymorphism information content(PIC) Genetic diversity

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Multi-scale processes influencing global carbon storage and land-carbon-climate nexus:A critical review

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Pedosphere 2023年第2期33卷 250-267页

作者： Owais Ali wani Shamal Shasang KUMAR Nazir hussain Anas Ibni Ali wani Subhash BABU Parvej ALAM Megna RASHID Simona Mariana POPESCU Sheikh MANSOOR Division of Soil Science and Agricultural Chemistry Sher-e-Kashmir University of Agricultural Sciences and Technology(SKUAST)Kashmir 193201(India) Division of Agronomy SKUASTKashmir 193201(India) Shri Mata Vaishno Devi University Katra 182301(India) Division of Agronomy ICAR-Indian Agricultural Research InstituteNew Delhi 110012(India) Department of Biology College of Science and HumanitiesPrince Sattam Bin Abdulaziz UniversityAlkharj 11942(Saudi Arabia) Faculty of Forestry SKUASTKashmir 191201(India) Department of Biology and Environmental Engineering University of CraiovaCraiova 200585(Romania) Division of Biochemistry Faculty of Basic SciencesSKUASTJammu 180009(India)

Carbon(C)is a key constitutive element in living organisms(plants,microbes,animals,and humans).Carbon is also a basic component of agriculture because it plays a dynamic role in crop growth,development,nutrient cycling,soil fertility,and other agricultural *** presence of C enhances soil physical,chemical,and biological *** C cycle supports all life on the Earth by transferring C between living organisms and the *** global climate is changing,and this change is attributable to the release of carbon dioxide and other greenhouse gases from human *** to the global climate change,agriculture is expected to be majorly *** production is directly linked to the *** five main global C pools are the oceanic,geologic,pedologic,atmospheric,and biotic pools,with specific reservoirs and inter-pool *** soil organic matter has various organic C pools(active,slow,and passive pools),containing various C-based fractions and specific liability ***,geology,land use,and management techniques are some of the variables that affect organic C and its *** dynamics of each of these variables must be understood for a thorough knowledge of how they impact the soil C pools and storage capacity under the changing climate *** review provides a comprehensive overview of the various factors that affect soil C pools/fractions and their C sequestration capacity.

关键词： carbon dynamics carbon pools carbon sequestration climate change climate-smart agriculture land use management practice soil organic carbon

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Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale×wheat derived doubled haploid lines

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BIOCELL 2021年第6期45卷 1651-1659页

作者： MUNIYANDI SAMUEL JEBERSON HARINDER KUMAR CHAUDHARY RAKESH KUMAR CHAHOTA SHABIR hussain wani AICRP(MULLaRP) Directorate of ResearchCAUImphalIndia Molecular Cytogenetics and Tissue Culture Laboratory CSKHPKVPalampurIndia Department of Agricultural Biotechnology CSKHPKVPalampurIndia Mountain Research Center for Field Crops Sher-e-Kashmir University of Agricultural Sciences and TechnologyKhudwaniIndia

The rye genome has shown potential for improvement of bread wheat,where wheat-rye substitutions and translocations have been and are frequently used in resistance *** belongs to different generations viz.,BC_(1)F_(1),BC_(1)F_(2),BC_(1)F_(3),BC_(1)F_(4)and BC_(2)F_(3)of triticale×wheat derived were used for different haploid induction parameters using Gogon grass(Imperata cylindrica)as a pollen *** percentage of pseudo seed formation ranged from 34.55%for BC_(1)F_(2)to 63.77 for BC_(1)F_(1)crosses,the haploid embryo formation ranges from 9.43%for BC_(1)F_(1)to 30.2%for BC_(1)F_(2),the haploid plant generation ranges from 19.36%for BC_(1)F_(2)to 63.25%for BC_(1)F_(1).Four doubled haploids were developed from ITSN 105/58-VL 802-VL 802 of BC_(2)F_(3)underwent molecular cytogenetic analyses using the probes,viz.,rye genomic rDNA,pSc 119 and *** and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.

关键词： Haploid Triticale×wheat backcross FISH GISH Rye chromatin

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Some Results on the Growth of Polynomials

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Analysis in Theory and Applications 2017年第4期33卷 316-322页

作者： Abdullah Mir Ajaz wani Imtiaz hussain Department of Mathematics University of Kashmir

In this paper, we have studied the Lacunary type of polynomials and proved a result which generalizes as well as refines some well-known polynomial inequalities regarding the growth of polynomials not vanishing inside... 详细信息

关键词： Lacunary polynomial Growth Bernstien-inequality.

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A Note on a Theorem of ***

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Analysis in Theory and Applications 2018年第4期34卷 293-296页

作者： Abdullah Mir Ajaz wani Imtiaz hussain Department of Mathematics University of KashmirSrinagar190006India

In this paper, we obtain a result that improves the results of Govil and Nwaeze, Qazi and the classical result of Rivlin.

关键词： Polynomial maximum modulus zeros

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Functional Role of miRNAs: Key Players in Soybean Improvement

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Phyton-International Journal of Experimental Botany 2021年第5期90卷 1339-1362页

作者： Kapil Gupta Sujit Kumar Mishra Shubhra Gupta Saurabh Pandey Jogeswar Panigrahi Shabir hussain wani CSIR-Central Institute of Medicinal and Aromatic Plants Lucknow226015India Department of Zoology School of Applied SciencesCenturion University of Technology and ManagementBhubaneswar751009India Department of Biotechnology Central University of RajasthanAjmer305817India Plant Molecular Biology International Center for Genetic Engineering&BiotechnologyNew Delhi110067India Department of Biosciences and Bioinformatics Khallikote UniversityBerhampur761008India Mountain Research Center for Field Crops KhudwaniSher-e-Kashmir University of Agricultural Sciences and Technology of KashmirAnantnag192101India

Soybean(Glycine max(L.)Merr)is an agro-economic crop growing across the world to cater nutrition for both human and animal feed due to the high oil and protein content in its edible *** genes and QTLs associated with important agronomic traits in this crop have already been identified and validated for soybean cyst nematode(SCN),Phytophthora root and stem rot,Pythium root rot and aphid resistance,seed quality,nutrient values,and also employed for genetic improvement in *** the last decade,micro RNAs(miRNAs)have been considered the effector molecules,so the detection and characterization of novel miRNAs in soybean have been taken up by several *** advancement in the strategy of sequencing and tools of bioinformatics during last decade has contributed to the discovery of many soybean miRNAs,thus miRNA can be used as a tool in molecular breeding studies,and this has opened new vistas for miRNA mediated genetic improvement of soybean to augment crop productivity as well as nutritional *** review addresses the current state of understanding of miRNAmediated stress responses,nutrient acquisition,plant development and crop production processes in soybean.

关键词： Soybean miRNA quality improvement plant development genetic engineering molecular breeding

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Morphometric Analysis of Foramen Magnum in the Determination of Sex Using Computed Tomography

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Journal of Forensic Science and Medicine 2021年第1期7卷 9-13页

作者： Bashir Ahmad wani Nusrat Nazir Rayees Ahmad Sheikh Altai hussain Chalkoo Tauseefa Jan Department of Oral Medicine and Radiology Government Dental CollegeSrinagarJammu and KashmirIndia

Background:sex identification by morphological assessment of various bones of the skeleton had been one of the oldest approaches in forensic anthropology and medico-legal *** and Objective:The aim of this study was to make the morphometric measurements of the foramen magnum in determining the feasibility of sex determination using computed tomography(CT).Materials and Methods:The data for our study was obtained from 100 patients having CT scan of the head and neck region(Siemens Somatom 256 slice CT scanner)with 1mm contiguous axial scans using bone window settings(2000 HU with 400 HU in centre),scan time of one second and exposure parameters 140 kVp and *** different measurements taken include the antero-posterior(AP)diameter,transverse diameter and area of the foramen ***:There were 50 males and 50 females with the age range of 18 to 75 *** have a higher average value than females in all of the parameters measured on foramen *** using Studenfs T-test,all the measured parameters showed significant difference between the sexes(p<0.01).Conclusion:The expression of sexual dimorphism in the foramen magnum region shows significant difference between the sexes,therefore this area of the skull should be considered a useful area in the identification of sex.

关键词： Computed tomography foramen magnum sex determination sexual dimorphism

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Inoculation of Chlorella and Food Waste Improves the Physio-Morphological Features of Red Pepper by Regulating Activating Antioxidant Defense System

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Phyton-International Journal of Experimental Botany 2023年第9期92卷 2699-2711页

作者： Sang-Mo Kang Shifa Shaffique Muhammad Imran Su-Mi Jeon Shabir hussain wani Muhammad Aaqil Khan Peter Odongkara Eun-Hae Kwon Yosep Kang Joon-Ik Son Won-Chan Kim In-Jung Lee Department of Applied Biosciences Kyungpook National UniversityDaegu41566Korea Biosafety Division National Institute of Agriculture ScienceRural Development AdministrationJeonju54874Korea Department of Technical Support Daegu City Agricultural Technology CenterDaegu41159Korea Department of Chemical and Life Sciences Qurtuba University of Science and Information TechnologyPeshawarPakistan Mountain Research for Field Crops Khudwani Shere-e-Kashmir University of Agriculture Sciences and Technology SrinagarJamu and KashmirIndia Corporate Affiliated Research Institute Seyen Co.Ltd.Kyungsan38561Korea

Food waste is recognized as a valuable source for potential agricultural applications to supply organic matter and nutrients to arable ***,the information on the combined application of food waste and the plant growth-promoting bacterial strain,Chlorella,related to plant metabolic features and sodium chloride content in arable soil is *** present study was conducted to investigate the exogenous application of food waste along with Chlorella,which improved the physio-morphological features of red *** results revealed that this combination enhanced the organic matter in the soil,ultimately improving the fertility rate of the soil,and the physio-morphological features,such as chlorophyll a content(24.5±0.7),root(7.8±0.7)cm and shoot length(12.1±0.7)cm,fresh weight(2.1±0.05)g,dry weight(0.19±0.05)g,mineral contents,and hormonal concentration(ABA by up to 2 folds).The combined treatment also minimized free radicals via the activation of the intrinsic antioxidant series cascade and electrolyte *** findings showed that adding Chlorella and food wastes improved growth characteristics and can be used as a green bio-fertilizer for sustainable agriculture.

关键词： Food waste Chlorella hormone antioxidant

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