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Photofermentative hydrogen production by immobilized Rhodopseudomonas sp. S16-VOGS3 cells in photobioreactors

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Energy Reviews 2024年第1期3卷 39-48页

作者： isabela C.Moia Aikaterini Kanaropoulou Demetrios f.Ghanotakis Pietro Carlozzi Eleftherios Touloupakis Research Institute on Terrestrial Ecosystems National Research CouncilVia Madonna del Piano 1050019Sesto FiorentinoFIItaly Department of Chemistry University of CreteVoutes Campus70013 HeraklionGreece

One of the most important solutions to overcome energy and environmental problems and to replace the fossil fuel-based economy could be the use of photosynthetic *** use of photosynthetic microorganisms is a potential alternative to energy generation from fossil fuels because they efficiently produce hydrogen(H_(2)).Immobilization of photosynthetic microorganisms is used for many biotechnological applications such as H_(2) *** method appears attractive because it restricts cell movement in an entrapped *** of Rhodopseudomonas sp.S16-VOGS3 cells is a promising way to improve H_(2) *** this work,the ability of immobilized Rhodopseudomonas sp.S16-VOGS3 cells to produce H_(2) was investigated in two types of *** PBRs used in this work were a cylindrical one with 0.2 L working volume(C-PBR)and a flat Roux type with 0.6 L working volume(fRT-PBR).The calcium alginate beads prepared were resistant to culture mixing and showed little leakage of cells,and the immobilized cells continued the photofermentation process in both *** immobilized cells in the C-PBR produced 936.8 mL of H_(2) with an average H_(2) production rate of 2.99 mL/*** average productivity was 126.4μL(H_(2))/mg(cells)/h or 14.96 mL(H_(2))/L(culture)/h,and the light conversion efficiency was 2.37%.The immobilized cells in the fRT-PBR produced a total of 662.2 mL of H_(2) with an average H_(2) production rate of 1.55 mL/*** average productivity was 31.1μL(H_(2))/mg(cells)/h or 2.58 mL(H_(2))/L(culture)/h,and the light conversion efficiency was 0.52%.The more uniform and therefore more efficient degree of bacterial cell mixing achieved in the C-PBR with cylindrical configuration played an important role compared to the *** the fRT-PBR,the beads were aggregated at the bottom,which limited light penetration and resulted in low H_(2) production efficiency.

关键词： Photobioreactor Rhodopseudomonas sp.S16-VOGS3 Photofermentation Hydrogen production Calcium alginate

Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

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World Journal of Methodology 2024年第1期14卷 114-123页

作者： Carolline Santos Miranda Daiana Araujo Santana-Oliveira isabela Lopes Vasques-Monteiro Nathan soares Dantas-Miranda Jade Sancha de Oliveira Glauser flavia Maria Silva-Veiga Vanessa Souza-Mello Department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil

BACKGROUND Excessive saturated fat intake compromises the integrity of the intestinal mucosa,leading to low-grade inflammation,impaired mucosal integrity,and increased intestinal permeability,resulting in the migration of lipopolysaccharide(LPS)to other *** To evaluate the chronic effects(at 10 and 16 wk)of a high-fat diet(HfD)(with 50%energy as fat)on the phylogenetic gut microbiota distribution and intestinal barrier structure and protection in C57BL/6 *** forty adult male mice were divided into four nutritional groups,where the letters refer to the type of diet(control and HfD or Hf)and the numbers refer to the period(in weeks)of diet administration:Control diet for 10 wk,HfD for 10 wk,control diet for 16 wk,and HfD for 16 *** sacrifice,biochemical,molecular,and stereological analyses were *** The Hf groups were overweight,had gut dysbiosis,had a progressive decrease in occludin immunostaining,and had increased LPS *** progression reduced the number of goblet cells per large intestine area and Mucin2 expression in the Hf16 group,consistent with a completely disarranged intestinal ultrastructure after 16 wk of HfD *** Chronic HfD intake causes overweight,gut dysbiosis,and morphological and functional alterations of the intestinal barrier after 10 or 16 ***-dependent reductions in goblet cell numerical density and mucus production have emerged as targets for countering obesity-driven intestinal damage.

关键词： High-fat diet Intestine Ultrastructure Goblet cells Gut microbiota

Model Identification and Control of Electromagnetic Actuation in Continuous Casting Process With Improved Quality

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IEEE/CAA Journal of Automatica Sinica 2023年第1期10卷 203-215页

作者： isabela Birs Cristina Muresan Dana Copot Clara Ionescu Research Group of Dynamical Systems and Control Faculty of Engineering and ArchitectureGhent University and with EEDT Decision&ControlFlanders Make ConsortiumTech Lane Science Park 125 and 131B-9052 GhentBelgium.I.Birs the Automation Department Faculty of Automation and Applied InformaticsTechnical University of Cluj-NapocaRomania IEEE

This paper presents an original theoretical framework to model steel material properties in continuous casting line process. Specific properties arising from non-Newtonian dynamics are herein used to indicate the natural convergence of distributed parameter systems to fractional order transfer function models. Data driven identification from a real continuous casting line is used to identify model of the electromagnetic actuator device to control flow velocity of liquid steel. To ensure product specifications, a fractional order control is designed and validated on the system. A projection of the closed loop performance onto the quality assessment at end production line is also given in this paper.

关键词： Electromagnetic actuator fractional order control fractional order system model non-Newtonian material

Microbial lipases:Propitious biocatalysts for the food industry

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food Bioscience 2022年第1期45卷 34-44页

作者： Cleonice Aparecida Salgado Clarissa isabela Aparecida dos Santos Maria Cristina Dantas Vanetti Department of Microbiology Universidade Federal de Viçosa(UFV)ViçosaMGBrazil

Lipases(triacylglycerol acylhydrolases,EC 3.***.1.3)are one of the largest groups of enzymes and are used in various industrial *** of microbial origin are currently receiving increased attention for industrial application as microorganisms grow quickly and are easily genetically ***,they offer several advantages,such as catalysis of diverse reactions,high specificity,high yields,low energy consumption and reduced processing time and production *** is a relentless ongoing effort to optimise the production of microbial lipases for potential application in the food *** this context,this review highlights the most promising techniques for producing microbial lipases and the recent applications of these lipases in dairy,oils and fats,bakery and confectionery,meat,flavours and aromas and other food *** lipases are normally obtained by fermentation,but the high costs of carbon and nitrogen sources limit the *** overcome this problem,low-cost agro-industrial residues in the lipase production process are *** obtain lipases with high yields and improved characteristics,the technique of protein engineering is described as promising,and the immobilization method that allows the recycling of lipases to improve their catalytic performance is *** to their catalytic properties and versatility,lipases of microbial origin are considered extremely important catalysts in the food industry,meeting the demand for tastier foods with pleasant aromas and ***,microbial lipases are considered safe and sustainable biocatalysts.

关键词： Microbial lipases food industry Agro-industrial residue

A perspective on potassic and ultrapotassic rocks:Constraints on the Paleoproterozoic late to post-collisional event in the São francisco paleocontinent

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Geoscience frontiers 2022年第5期13卷 242-266页

作者： Samuel Moreira Bersan AndréDanderfer Craig Storey Henrique Bruno Hugo Moreira francisco Abreu Cristiano Lana Leonardo Gonçalves isabela Nahas Universidade federal de Ouro Preto Departamento de GeologiaCampus Morro do CruzeiroOuro PretoMG CEP 35400-000Brazil University of Portsmouth School of the EnvironmentGeography and GeosciencesBurnaby BuildingBurnaby RoadPortsmouth PO13QLUK Tektos Research Group UERJ-Rio de Janeiro State UniversityBrazil Sustain Geologia Belo HorizonteBrazil

The late-to post-collisional stage in orogenic systems is characterized by the coeval existence of bimodal potassic to ultrapotassic magmatic activity related to partial melting of an enriched lithospheric mantle together with crustal derived *** this paper,we present new whole rock geochemical analyses combined with zircon and titanite U–Pb and zircon Hf isotopic data from potassic to ultrapotassic rocks from six plutons that occur within the Archean Itacambira-Monte Azul block(BIMA),to discuss their petrogenesis and the tectonic implications for the São francisco *** new U–Pb ages range from ca.2.06 Ga to 1.98 Ga and reveal long-lasting potassic magmatism within the BIMA,which is within the late-to-post-collisional stage of the São francisco paleocontinent *** ultrapotassic rocks are compatible with a fluid-related metasomatized mantle source enriched by previous subduction events,whereas the potassic rocks are bimodal and have a transitional shoshonitic to A-type *** rocks have a hybrid nature,possible related to the mixing between the mafic potassic/ultrapotassic rocks and high temperature crustal melts of the Archean continental *** results also show an increase of within-plate signature towards the younger potassic *** participation of an important Archean crustal component in the genesis of these rocks is highlighted by the common and occasionally abundant occurrence of Archean inherited *** Hf isotopic record shows that most of the zircon inheritance has dominantly subchondriticεHf(t)values,which fits a crustal reworking derivation from a similar Eo-to Paleoarchean precursor ***,the presence of juvenile 2.36 Ga zircon inheritance in an ultrapotassic sample reveal the existence of a hidden reservoir that is somewhat similar to the described for the Mineiro Belt in southern São francisco paleocontinent.

关键词： Late-to post-collisional Potassic-ultrapotassic rocks U–Pb dating São francisco paleocontinent Rhyacian-Orosirian orogenic system

Peroxisome proliferator-activated receptor-alpha activation and dipeptidyl peptidase-4 inhibition target dysbiosis to treat fatty liver in obese mice

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World Journal of Gastroenterology 2022年第17期28卷 1814-1829页

作者： flavia Maria Silva-Veiga Carolline Santos Miranda isabela Macedo Lopes Vasques-Monteiro Henrique Souza-Tavares fabiane ferreira Martins Julio Beltrame Daleprane Vanessa Souza-Mello Department of Anatomy Institute of BiologyState University of Rio de JaneiroLaboratory of MorphometryMetabolism and Cardiovascular DiseasesRio de Janeiro 20551-030Brazil Department of Clinical and Toxicology Analysis State University of Rio de JaneiroNutrition InstituteState University of Rio de JaneiroNutrition InstituteUniversity of Sao PauloRio de Janeiro 20551-030Brazil

BACKGROUND Obesity and comorbidities onset encompass gut dysbiosis,altered intestinal permeability,and *** that target gut dysbiosis can cope with obesity and nonalcoholic fatty liver disease(NAfLD)*** proliferator-activated receptor(PPAR)-alpha activation and dipeptidyl-peptidase-4(DPP-4)inhibition alleviate NAfLD,but the mechanism may involve gut microbiota modulation and merits further *** To address the effects of PPAR-alpha activation and DPP-4 inhibition(isolated or combined)upon the gut-liver axis,emphasizing inflammatory pathways in NAfLD management in high-fat-fed C57BL/6J *** Male C57BL/6J mice were fed a control diet(C,10%of energy as lipids)or a highfat diet(HfD,50%of energy as lipids)for 12 wk,when treatments started,forming the groups:C,Hf,HfA(HfD+PPAR-alpha agonist WY14643,2.5 mg/kg body mass),HfL(HfD+DPP-4 inhibitor linagliptin,15 mg/kg body mass),and HfC(HfD+the combination of WY14643 and linagliptin).RESULTS The HfD was obesogenic compared to the C *** treatments elicited significant body mass loss,and the HfC group showed similar body mass to the C *** treatments tackled oral glucose intolerance and raised plasma glucagon-like peptide-1 *** metabolic benefits restored Bacteroidetes/firmicutes ratio,resulting in increased goblet cells per area of the large intestine and reduced lipopolysaccharides concentrations in treated *** the gene level,treated groups showed higher intestinal Mucin 2,Occludin,and Zo-1 expression than the HfD *** reduced endotoxemia suppressed inflammasome and macrophage gene expression in the liver of treated *** observations complied with the mitigation of liver steatosis and reduced hepatic triacylglycerol,reassuring the role of the proposed treatments on NAfLD *** PPAR alpha activation and DPP-4 inhibition(isolated or combined)tackled NAfLD in dietinduced obese mice by restoration of gut-liver axis

关键词： Nonalcoholic fatty liver disease High-fat diet Peroxisome proliferator-activated receptor-alpha Dipeptidyl-peptidase-4-inhibitor Dysbiosis Inflammation

Coronavirus disease 2019 severity in obesity:Metabolic dysfunctionassociated fatty liver disease in the spotlight

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World Journal of Gastroenterology 2021年第16期27卷 1738-1750页

作者： isabela Macedo Lopes Vasques-Monteiro Vanessa Souza-Mello Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil food Science and Technology Federal University of the State of Rio de JaneiroRio de Janeiro 22290250Brazil Laboratory of Morphometry Metabolismand Cardiovascular DiseasesBiomedical CenterInstitute of BiologyAnatomy DepartmentRio de Janeiro State UniversityRio de Janeiro 20551030Brazil

The coronavirus disease 2019(COVID-19)outbreak has drawn the scientific community's attention to pre-existing metabolic conditions that could aggravate the infection,causing extended viral shedding,prolonged hospitalization,and high death *** dysfunction-associated fatty liver disease(MAfLD)emerges as a surrogate for COVID-19 severity due to the constellation of metabolic alterations it *** review outlines the impact MAfLD exerts on COVID-19 severity in obese subjects,besides the possible mechanistic links to the poor *** data collected showed that MAfLD patients had poorer COVID-19 outcomes than non-MAfLD obese *** is generally accompanied by impaired glycemic control and systemic arterial hypertension,both of which can decompensate during the COVID-19 clinical ***,MAfLD subjects had higher plasma inflammatory marker concentrations than non-MAfLD subjects,which might be related to an intensified cytokine storm syndrome frequently associated with the need for mechanical ventilation and *** conclusion,MAfLD represents a higher risk than obesity for COVID-19 severity,resulting in poor outcomes and even progression to non-alcoholic *** should include MAfLD subjects in the high-risk group,intensify preventive measurements,and prioritize their vaccination.

关键词： Metabolic dysfunction-associated fatty liver disease Obesity COVID-19 Severity Cytokine storm syndrome

Sleep in children with eosinophilic esophagitis

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World Journal of Pediatrics 2021年第2期17卷 111-114页

作者： isabela A Ishikura Gustavo Moreira SergioTufik Monica L.Andersen Department of Psychobiology Universidade Federal de Sao Paulo(UNIFESP)Napoleao de Barros925 Vila ClementinoSao PauloSP 04024-002Brazil

Eosinophilic esophagitis(EoE)is a chronic immune-and antigen-mediated disease caused by infiltration of eosino-phils in esophagus tissue,which leads to morphological and functional abnormalities at this site[1].This disease was discovered in 1995 by Kelly et al.[2];since then,it has become increasingly recognized and discussed by research-ers[2,3].EoE affects children and adults and is three times more prevalent in males than in *** to a recent systematic review,there are 19.1 cases of EoE per 100,000 children/year,with a higher frequency in Western countries[4].

关键词： esophagitis al. esophagus

Peroxisome proliferator-activated receptors as targets to treat metabolic diseases:focus on the adipose tissue,liver,and pancreas

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World Journal of Gastroenterology 2023年第26期29卷 4136-4155页

作者： Henrique Souza-Tavares Carolline Santos Miranda isabela Macedo Lopes Vasques-Monteiro Cristian Sandoval Daiana Araujo Santana-Oliveira flavia Maria Silva-Veiga Aline fernandes-da-Silva Vanessa Souza-Mello Department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil Escuela de Tecnología Médica Facultad de SaludUniversidad Santo TomásOsorno 5310431Chile Departamento de Ciencias Preclínicas Universidad de la FronteraTemuco 4780000Chile

The world is experiencing reflections of the intersection of two pandemics:Obesity and coronavirus disease *** prevalence of obesity has tripled since 1975 worldwide,representing substantial public health costs due to its *** adipose tissue is the initial site of obesity *** excessive energy intake,it undergoes hyperplasia and hypertrophy until overt inflammation and insulin resistance turn adipocytes into dysfunctional cells that send lipotoxic signals to other *** pancreas is one of the organs most affected by *** lipotoxicity becomes chronic,there is an increase in insulin secretion by pancreatic beta cells,a surrogate for type 2 diabetes mellitus(T2DM).These alterations threaten the survival of the pancreatic islets,which tend to become dysfunctional,reaching exhaustion in the long *** for the liver,lipotoxicity favors lipogenesis and impairs beta-oxidation,resulting in hepatic *** silent disease affects around 30%of the worldwide population and can evolve into end-stage liver *** therapy for hepatic steatosis remains to be defined,peroxisome proliferator-activated receptors(PPARs)activation copes with T2DM *** PPARs are transcription factors found at the intersection of several metabolic pathways,leading to insulin resistance relief,improved thermogenesis,and expressive hepatic steatosis mitigation by increasing mitochondrial *** review aimed to update the potential of PPAR agonists as targets to treat metabolic diseases,focusing on adipose tissue plasticity and hepatic and pancreatic remodeling.

关键词： Obesity Insulin resistance Peroxisome proliferator-activated receptors Pancreas Hepatic steatosis Adipose tissue