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The cotton mitochondrial chimeric gene orf610a causes male sterility by disturbing the dynamic balance of ATP synthesis and ROS burst

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The Crop Journal 2022年第6期10卷 1683-1694页

作者： Yongjie Zhang Yang Han Meng Zhang Xuexian Zhang Liping Guo Tingxiang Qi Yongqi Li Juanjuan Feng Hailin Wang huini tang Xiuqin Qiao Liangliang Chen Xiatong Song Chaozhu Xing Jianyong Wu Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001HenanChina State Key Laboratory of Cotton Biology/Key Laboratory for Cotton Genetic Improvement Ministry of AgricultureInstitute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina State Key Laboratory of Cotton Biology(Hebei Base) College of AgronomyHebei Agricultural UniversityBaoding 071001HebeiChina

Plant cytoplasmic male sterility(CMS)is maternally inherited and often manifested as aborted pollen development,but the molecular basis of abortion remains to be *** facilitate an investigation of CMS in cotton,the complete sequence of cotton mitochondrial(mt)genome for CMS-D2 line ZBA was *** mt genome was assembled as a single circular molecule with 634,036 bp in length.A total of 194 ORFs,36 protein-coding genes,six r RNAs,and 24 t RNAs were *** chimeric genes encoding hypothetical proteins with transmembrane domains were *** them,a previously unknown chimeric gene,orf610a,which is composed of atp1 and a 485-bp downstream sequence of unknown nature,was ***-PCR and q RT-PCR validation indicated that orf610a was expressed specifically in a sterile *** expression of orf610a in yeast resulted in excessive accumulation of reactive oxygen species and reduction in ATP content,in addition to inhibition of cellular *** *** overexpressing orf610a fused with a mitochondrial targeting peptide displayed partial male *** between ORF610a and the nuclear-encoded protein RD22 indicated an association between ORF610a and pollen *** feedback during transcriptional regulation between nuclear regulatory factors and the mt CMS gene may account for the male sterility of ZBA.

关键词： Cytoplasmic male sterility Mitochondrial genome Chimeric gene Reactive oxygen species ATP

Genome-wide analysis of Rf-PPR-like(RFL)genes and a new InDel marker development for Rf1 gene in cytoplasmic male sterile CMS-D2 Upland cotton

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Journal of Cotton Research 2018年第3期1卷 9-19页

作者： ZHANG Bingbing ZHANG Xuexian GUO Liping QI Tingxiang WANG Hailin tang huini QIAO Xiuqin SHAHZAD Kashif XING Chaozhu WU Jianyong State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Background: Cytoplasmic male sterility in flowering plants is a convenient way to use heterosis via hybrid breeding and may be restored by nuclear restorer-of-fertility(Rf) genes. In most cases, Rf genes encoded pentatricopeptide repeat(PPR) proteins and several Rf genes are present in clusters of similar Rf-PPR-like(RFL) genes. However, the Rf genes in cotton were not fully characterized until ***: In total, 35 RFL genes were identified in G. hirsutum, 16 in G. arboreum, and 24 in G. raimondii. Additionally,four RFL-rich regions were identified; the RFL-rich region in Gh05 is the probable location of Rf-PPR genes in cotton and will be studied further in the future. Furthermore, an insertion sequence was identified in the promoter sequence of Gh05 G3392 gene in the restorer line, as compared with the CMS-D2 line and maintainer lines. An InDel-R marker was then developed and could be used to distinguish the restorer line carrying Rfl from other genotypes without the Rf1 ***: In this study, genome-wide identification and analysis of RFL genes have identified the candidate Rf-PPR genes for CMS in Gossypium. The identification and analysis of RFL genes and sequence variation analysis will be useful for cloning Rf genes in the future and also for three-line hybrid breeding in cotton.

关键词： Upland cotton CMS Rf-PPR-like gene Restorer gene InDel marker

Genetic analysis of yield and fiber quality traits in upland cotton(Gossypium hirsutum L.) cultivated in different ecological regions of China

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Journal of Cotton Research 2019年第2期2卷 122-132页

作者： SHAHZAD Kashif LI Xue QI Tingxiang GUO Liping tang huini ZHANG Xuexian WANG Hailin ZHANG Meng ZHANG Bingbing QIAO Xiuqin XING Chaozhu WU Jianyong State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural Sciences/Key Laboratory for Cotton Genetic ImprovementMinistry of Agriculture and Rural Affairs

Background:Cotton is an important fiber crop *** yield potential of current genotypes of cotton can be exploited through ***,to develop superior hybrids with high yield and fiber quality traits,information of genetic control of traits is ***,genetic analysis plays pivotal role in plant ***:In present study,North Carolina II mating design was used to cross 5 female parents with 6 male parents to produce 30 intraspecific F1cotton *** plant materials were tested in three different ecological regions of China during the year of ***-dominance-environment(ADE)genetic model was used to estimate the genetic effects and genotypic and phenotypic correlation of yield and fiber quality *** showed that yield traits except lint percentage were mainly controlled by genetic and environment interaction effects,whereas lint percentage and fiber quality traits were determined by main genetic ***,dominant and additiveen vironine nt in teraction effects had more influence on yield traits,whereas additive and domi nance-e nviron ment interaction effects were found to be predominant for fiber ***-sense and its interaction heritability were significant for all yield and most of fiber quality ***-sense and its interaction heritability were non-significant for boll number and seed cotton *** analysis indicated that seed cotton yield had significant positive correlation with other yield attributes and non-significant with fiber quality *** fiber quality traits had signiflcant positive correlation with each other except ***:Results of current study provide important information about genetic control of yield and fiber quality ***,this study identified that parental lines,e.g.,SJ48-1,ZB-1,851-2,and DT-8 can be utilized to improve yield and fiber quality traits in cotton.

关键词： Cotton Hybrid Genetic effects Additive Dominance Correlation

Comparative performance of hybrid generations reveals the potential application of F_(2)hybrids in upland cotton

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Journal of Cotton Research 2022年第2期5卷 193-206页

作者： CHEN Liangliang tang huini ZHANG Xuexian QI Tingxiang GUO Liping SHAHZAD Kashif WANG Hailin QIAO Xiuqin ZANG Rong ZHANG Meng WU Jianyong XING Chaozhu State Key Laboratory of Cotton Biology In stitute of Cotton Research of Chinese Academy of Agricultural SciencesKey Laboratory for Cotton Genetic ImprovementMinistry of Agriculture and Rural AffairsAnyang 455000HenanChina

Background:The utilization of heterosis has greatly improved the productivity of cotton ***,a major constraint for the large-scale promotion of F_(1) hybrid cotton is artificial emasculation and *** study proposed the potential utilization of F_(2) hybrids to improve upland cotton production through a comparative evaluation of hybrid ***:Eight upland cotton varieties were analyzed and crosses were made according to NCII incomplete diallel cross-breeding design in two cotton belts of *** analysis revealed significant differences in agronomic,yield,and fiber quality in both generations and *** broad-sense heritability of agronomic and yield traits was relatively higher than quality ***,the narrow-sense heritability of some traits was higher in F_(2) than in the F_(1) generation in both cotton ***,parental lines Zhong901,ZB,L28,and Z98 were observed with maximum combining ability while combinations with strong special combining ability were ZB×DT,L28×Z98,and ZB×*** yield traits heterosis was predominant in both ***,the level of heterosis was altered with trait,hybrid combination,generation,and ***,L28×Z98 performed outstandingly in *** lint yield(LY)was 24.2%higher in F_(1) and 11.6%in F_(2) than that of the control Ruiza *** performance of SJ48×Z98 was excellent in Aral which showed 36.5%higher LY in F_(2)and 10.9%in F_(2)than control CCRI *** results revealed most hybrid combinations had shown a low level of heterosis for agronomic and fiber quality traits in both ***,ZB×DT and L28×Z98 showed hybrid vigor for multiple traits in both generations and cotton *** is feasible to screen strong heterosis hybrid combinations with fine fiber in early *** the two environments,the correlation of some traits showed the same trend,and the correlation degree of Anyang site was higher than that

关键词： Upland cotton F_(2)generation Combining ability Heterosis Heritability

三裂棉不育胞质杂交组合F_(1)、F_(2)产量优势分析

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中国棉花 2024年第1期51卷 4-10页

作者：唐会妮张梦王舜戚廷香吴建勇郭立平张学贤陈亮亮王海林乔秀琴梅拥军邢朝柱塔里木大学农学院新疆阿拉尔843300 中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室/农业农村部棉花生物学与遗传育种重点实验室河南安阳455000 商丘市睢阳区乡村产业发展服务中心河南商丘476000

选用7个不同细胞核背景的三裂棉细胞质雄性不育系(CMS-D8)和1个具有强恢复力的哈克尼西棉雄性不育恢复系ZHR142作为亲本,配制7个杂交组合,分别种植在西北内陆棉区的新疆阿拉尔和黄河流域棉区的河南安阳,对亲本及其杂交组合F_(1)、F_(2)... 详细信息

选用7个不同细胞核背景的三裂棉细胞质雄性不育系(CMS-D8)和1个具有强恢复力的哈克尼西棉雄性不育恢复系ZHR142作为亲本,配制7个杂交组合,分别种植在西北内陆棉区的新疆阿拉尔和黄河流域棉区的河南安阳,对亲本及其杂交组合F_(1)、F_(2)和对照的产量及其构成因素进行优势分析。结果表明:尽管F_(2)产量优势较F_(1)有所衰退,但仍有部分组合如3、6和7的F_(2)籽棉产量和皮棉产量表现较好;大部分CMS-D8胞质F_(2)产量竞争优势减退主要是由于铃重的降低;部分组合如1、3、6和7的不同世代在2个生态点具有一定的产量优势,尤其是组合6和7的F_(1)、F_(2)产量在阿拉尔生态点均表现为正向竞争优势。本研究结果对D8胞质不育三系杂交棉选育及其F_(2)利用具有一定的指导意义。

关键词：棉花 CMS-D8 杂种优势杂种二代产量中亲优势超亲优势竞争优势优势衰退

棉花细胞质雄性不育恢复基因Rf_1候选区域SSCP标记开发与候选基因表达分析

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棉花学报 2018年第1期30卷 12-20页

作者：刘利彩郭立平戚廷香王海林唐会妮张学贤乔秀琴吴建勇邢朝柱棉花生物学国家重点实验室中国农业科学院棉花研究所河南安阳455000

【目的】棉花细胞质雄性不育恢复基因Rf_1的定位对于研究恢复基因的作用机制和改良恢复系具有重要意义。前期研究结果表明在恢复基因Rf_1所在Scaffold 333上的目标区间内有9个PPR(Pentatricopeptide repeat)基因成簇分布在160 kb区间内... 详细信息

【目的】棉花细胞质雄性不育恢复基因Rf_1的定位对于研究恢复基因的作用机制和改良恢复系具有重要意义。前期研究结果表明在恢复基因Rf_1所在Scaffold 333上的目标区间内有9个PPR(Pentatricopeptide repeat)基因成簇分布在160 kb区间内,此区间内还包括另外9个其他类型的基因。为进一步丰富恢复基因目标区域内标记数目,并了解相关基因的表达模式。【方法】利用这18个基因的序列设计出覆盖全部基因的引物共155对,通过单链构象多态性(Single-strand conformation polymorphism,SSCP)技术对可育池和不育池进行筛选,利用荧光定量聚合酶链式反应(Polymerase chain reaction,PCR)对目标区域内的8个非PPR基因在不育系、保持系、恢复系的花蕾不同发育时期的表达模式进行了分析。【结果】共得到15对多态性引物,结合前期的3个简单重复序列标记(Simple sequence repeats,SSR)标记对F_2群体进行基因型分析,结果表明这些标记分布在4.8 cM范围内。受恢复基因或不育胞质的影响,在花蕾4个发育时期,大部分基因表达存在明显差异。【结论】本研究获得了与恢复基因紧密连锁的SSCP标记,并初步了解了目标区间内部分基因的表达模式,为进一步开展恢复基因精细定位和候选基因的筛选提供了基础。

关键词：棉花恢复基因Rf1 遗传定位 PPR基因 SSCP标记

棉花细胞质雄性不育的胞质效应研究现状及未来展望

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棉花学报 2023年第5期35卷 429-438页

作者：李博张梦张学贤郭立平戚廷香唐会妮王瑞杰王海林乔秀琴吴建勇邢朝柱中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室/农业农村部棉花生物学与遗传育种重点实验室河南安阳455000

棉花具有明显的杂种优势,具体表现在产量、纤维品质、抗病虫等性状上。杂交种子生产是棉花杂种优势利用中非常重要的一环。近年来,由于人工杂交制种成本逐年提高,简化、高效、低成本的制种技术已经成为未来杂交棉发展的必然趋势。生产... 详细信息

棉花具有明显的杂种优势,具体表现在产量、纤维品质、抗病虫等性状上。杂交种子生产是棉花杂种优势利用中非常重要的一环。近年来,由于人工杂交制种成本逐年提高,简化、高效、低成本的制种技术已经成为未来杂交棉发展的必然趋势。生产实践表明,利用细胞质雄性不育系不仅能简化制种方法还可以节约劳动力成本,目前已成为作物杂种优势利用领域的研究热点。然而,不育细胞质对棉花形态建成、花药发育、产量形成和纤维发育等均有一定影响,并且存在不利于棉花生长发育的负效应,从而限制了“三系”(不育系、保持系和恢复系)杂交棉的进一步推广利用。为此,综述了细胞质雄性不育对棉花主要性状的影响及其负效应产生的分子基础,并初步探讨了克服棉花不育细胞质负效应的潜在途径,以期为生产上选育和改良棉花细胞质雄性不育恢复系和强优势“三系”杂交种提供新思路。

关键词：棉花细胞质雄性不育细胞质效应研究现状育种对策

陆地棉杂交组合F_(1)、F_(2)苗期优势表现及亲本配合力分析

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新疆农业科学 2023年第2期60卷 261-271页

作者：陈亮亮张梦郭立平戚廷香张学贤唐会妮王海林乔秀琴吴建勇邢朝柱中国农业科学院棉花研究所/棉花生物学国家重点实验室/农业农村部棉花生物学与遗传育种重点实验室河南安阳455000

【目的】筛选优异亲本和强优势杂交组合选育,为棉花F_(2)生产应用提供理论依据。【方法】以8个核背景不同的陆地棉材料为亲本,采用5×3的NCⅡ遗传交配设计,在大田环境条件下,测定亲本、F_(1)、F_(2)和对照品种苗期株高、叶片SPAD值和光... 详细信息

【目的】筛选优异亲本和强优势杂交组合选育,为棉花F_(2)生产应用提供理论依据。【方法】以8个核背景不同的陆地棉材料为亲本,采用5×3的NCⅡ遗传交配设计,在大田环境条件下,测定亲本、F_(1)、F_(2)和对照品种苗期株高、叶片SPAD值和光合作用参数。【结果】株高、SPAD值和净光合速率的广义遗传力和狭义遗传力较高,部分性状的F_(2)遗传力高于F_(1)。苗期各性状一般配合力好的亲本为P_(1)(中901)、P_(2)(ZB)、P_(6)(大桃)和P_(7)(Z98);杂交组合F_(1)和F_(2)在苗期性状(株高、SPAD值、净光合速率、气孔导度、胞间CO_(2)浓度、蒸腾速率)中特殊配合力表现较好的为组合2(中901×Z98)、组合5(ZB×Z98)、组合7(SJ48×DT)。15个杂交组合的F_(1)和F_(2)在苗期各性状中较对照表现出不同的竞争优势,部分组合具有显著性差异;多数杂交组合的F_(1)和F_(2)在株高、SPAD值、净光合速率和气孔导度表现出正向中亲优势和超亲优势,而大多数组合F_(1)和F_(2)的胞间CO_(2)浓度和蒸腾速率表现出明显的负向中亲优势和超亲优势。【结论】在苗期性状中由父本P 7(Z98)组配的杂交组合在后代表现较好,具有较大的利用潜力。

关键词：陆地棉杂交种苗期优势表现配合力

棉花雄性不育细胞质对ATP和H2O2含量的影响分析

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棉花学报 2022年第4期34卷 350-360页

作者：韩阳臧榕张梦陈亮亮张学贤郭立平戚廷香唐会妮王海林乔秀琴邢朝柱张艳吴建勇河北农业大学/棉花生物学国家重点实验室河北基地河北保定071001 中国农业科学院棉花研究所/棉花生物学国家重点实验室河南安阳455000

【目的】探究哈克尼西棉不育胞质(CMS-D2)和三裂棉不育胞质(CMS-D8)对ATP和H_(2)O_(2)含量的影响。【方法】选用CMS-D2和CMS-D8两套“三系”(不育系、保持系及其恢复系)材料,测定其不同发育时期的叶片和花蕾中ATP和H_(2)O_(2)含量;并结... 详细信息

【目的】探究哈克尼西棉不育胞质(CMS-D2)和三裂棉不育胞质(CMS-D8)对ATP和H_(2)O_(2)含量的影响。【方法】选用CMS-D2和CMS-D8两套“三系”(不育系、保持系及其恢复系)材料,测定其不同发育时期的叶片和花蕾中ATP和H_(2)O_(2)含量;并结合已有研究测定候选不育基因orf610a超表达拟南芥植株中ATP和H_(2)O_(2)含量;通过实时荧光定量聚合酶链式反应分析ATP合成相关基因在不育系、保持系以及转基因拟南芥花蕾中的表达量。【结果】与其保持系和恢复系相比,CMS-D2不育系叶片在蕾期和铃期均表现为ATP含量极显著降低、H_(2)O_(2)含量极显著升高;CMS-D8不育系叶片在蕾期表现为ATP含量极显著降低、H_(2)O_(2)含量极显著升高,在铃期表现为ATP含量极显著升高;CMS-D2不育系3 mm、4 mm花蕾中ATP含量极显著降低,4 mm花蕾中H_(2)O_(2)含量极显著上升;CMS-D8不育系5 mm、6 mm花蕾中ATP含量极显著降低。与其保持系相比,CMS-D2和CMS-D8不育系花蕾中,多个ATP合成相关基因的表达量极显著降低。与野生型材料相比,orf610a超表达拟南芥角果和花蕾中的H_(2)O_(2)含量显著升高,ATP含量极显著降低,且大多数ATP合成相关基因均极显著下调表达。【结论】不育胞质会对棉花不同发育时期的叶片和花蕾中ATP和H_(2)O_(2)含量产生一定的影响,二者动态平衡的破坏可能会影响花粉的正常发育。

关键词：棉花细胞质雄性不育 ATP含量 H2O2含量 orf610a