Genome-wide analysis of Rf-PPR-like(RFL)genes and a new InDel marker development for Rf1 gene in cytoplasmic male sterile CMS-D2 Upland cotton

作     者：ZHANG Bingbing ZHANG Xuexian GUO Liping QI Tingxiang WANG Hailin TANG Huini QIAO Xiuqin SHAHZAD Kashif XING Chaozhu WU Jianyong 

作者机构：State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences 

出 版 物：《Journal of Cotton Research》 (棉花研究（英文）)

年 卷 期：2018年第1卷第3期

页      面：9-19页

学科分类：09[农学] 

基　　金：financed by National Key Research and Development Program of China(2016YFD0101400) Foundation of State Key Laboratory of Cotton Biology(CB2018C06) 

主　　题：Upland cotton CMS Rf-PPR-like gene Restorer gene InDel marker 

摘      要：Background: Cytoplasmic male sterility in flowering plants is a convenient way to use heterosis via hybrid breeding and may be restored by nuclear restorer-of-fertility(Rf) genes. In most cases, Rf genes encoded pentatricopeptide repeat(PPR) proteins and several Rf genes are present in clusters of similar Rf-PPR-like(RFL) genes. However, the Rf genes in cotton were not fully characterized until ***: In total, 35 RFL genes were identified in G. hirsutum, 16 in G. arboreum, and 24 in G. raimondii. Additionally,four RFL-rich regions were identified; the RFL-rich region in Gh05 is the probable location of Rf-PPR genes in cotton and will be studied further in the future. Furthermore, an insertion sequence was identified in the promoter sequence of Gh05 G3392 gene in the restorer line, as compared with the CMS-D2 line and maintainer lines. An InDel-R marker was then developed and could be used to distinguish the restorer line carrying Rfl from other genotypes without the Rf1 ***: In this study, genome-wide identification and analysis of RFL genes have identified the candidate Rf-PPR genes for CMS in Gossypium. The identification and analysis of RFL genes and sequence variation analysis will be useful for cloning Rf genes in the future and also for three-line hybrid breeding in cotton.