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The miR157-SPL-CNR module acts upstream of bHLH101 to negatively regulate iron deficiency responses in tomato

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Journal of Integrative Plant Biology 2022年第5期64卷 1059-1075页

作者： huihui zhu Jiayi Wang Dan Jiang Yiguo Hong Jiming Xu Shaojian Zheng Jianli Yang Weiwei Chen State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China Research Centre for Plant RNA Signaling College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China

Iron(Fe)homeostasis is critical for plant growth,development,and stress responses.Fe levels are tightly controlled by intricate regulatory networks in which transcription factors(TFs)play a central role.A series of basic helix-loop-helix(b HLH)TFs have been shown to contribute to Fe homeostasis,but the regulatory layers beyond b HLH TFs remain largely unclear.Here,we demonstrate that the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE(SPL)TF Sl SPL-CNR negatively regulates Fe-deficiency responses in tomato(Solanum lycopersicum)roots.Fe deficiency rapidly repressed the expression of Sl SPL-CNR,and Fe deficiency responses were intensified in two clustered regularly interspaced palindromic repeats(CRISPR)/CRISPRassociated protein 9-generated Sl SPL-CNR knockout lines compared to the wild-type.Comparative transcriptome analysis identified 47 Fe deficiencyresponsive genes the expression of which is negatively regulated by Sl SPL-CNR,one of which,Slb HLH101,helps regulate Fe uptake genes.Sl SPLCNR localizes the nucleus and interacts with the GTAC and BOX 4(ATTAAT)motifs in the Slb HLH101 promoter to repress its expression.Inhibition of Sl SPL-CNR expression in response to Fe deficiency was well correlated with the expression of the micro RNA Slymi R157.Slymi R157-overexpressing tomato lines displayed enhanced Fe deficiency responses,as did Sl SPL-CNR loss-of-function mutants.We propose that the Slymi R157-Sl SPL-CNR module represents a novel pathway that acts upstream of Slb HLH101 to regulate Fe homeostasis in tomato roots.

关键词： Fe deficiency miR157 SPL-CNR tomato transcriptional repressor

Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 163-176页

作者： Feiyang Ji Menghao Zhou huihui zhu Zhengyi Jiang Qirui Li Xiaoxi Ouyang Yiming Lv Sainan Zhang Tian Wu Lanjuan Li State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003China The First Affiliated Hospital College of MedicineZhejiang UniversityHangzhou 310003China Department of Colorectal Surgery Sir Run Run Shaw HospitalCollege of MedicineZhejiang UniversityHangzhou 310003China Quzhou Second People’s Hospital Quzhou 324000China

Posttranslational modifications(PTMs)of proteins,particularly acetylation,phosphorylation,and ubiquitination,play critical roles in the host innate immune response.PTMs’dynamic changes and the crosstalk among them are complicated.To build a comprehensive dynamic network of inflammation-related proteins,we integrated data from the whole-cell proteome(WCP),acetylome,phosphoproteome,and ubiquitinome of human and mouse macrophages.Our datasets of acetylation,phosphorylation,and ubiquitination sites helped identify PTM crosstalk within and across proteins involved in the inflammatory response.Stimulation of macrophages by lipopolysaccharide(LPS)resulted in both degradative and non-degradative ubiquitination.Moreover,this study contributes to the interpretation of the roles of known inflammatory molecules and the discovery of novel inflammatory proteins.

关键词： Proteome Crosstalk Inflammation Lipopolysaccharide Macrophage

CG hypermethylation of the bHLH39 promoter regulates its expression and Fe deficiency responses in tomato roots

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Horticulture Research 2023年第7期10卷 125-137页

作者： huihui zhu Guanghao Han Jiayi Wang Jiming Xu Yiguo Hong Li Huang Shaojian Zheng Jianli Yang Weiwei Chen State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310058China Research Centre for Plant RNA Signaling College of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China Laboratory of Cell&Molecular Biology Institute of Vegetable ScienceZhejiang UniversityHangzhou 310058China

Iron(Fe)is an essential micronutrient for all organisms,including plants,whose limited bioavailability restricts plant growth,yield,and nutritional quality.While the transcriptional regulation of plant responses to Fe deficiency have been extensively studied,the contribution of epigenetic modulations,such as DNA methylation,remains poorly understood.Here,we report that treatment with a DNA methylase inhibitor repressed Fe deficiency-induced responses in tomato(Solanum lycopersicum)roots,suggesting the importance of DNA methylation in regulating Fe deficiency responses.Dynamic changes in the DNA methylome in tomato roots responding to short-term(12 hours)and long-term(72 hours)Fe deficiency identified many differentially methylated regions(DMRs)and DMR-associated genes.Most DMRs occurred at CHH sites under short-term Fe deficiency,whereas they were predominant at CG sites following long-term Fe deficiency.Furthermore,no correlation was detected between the changes in DNA methylation levels and the changes in transcript levels of the affected genes under either short-term or long-term treatments.Notably,one exception was CG hypermethylation at the bHLH39 promoter,which was positively correlated with its transcriptional induction.In agreement,we detected lower CG methylation at the bHLH39 promoter and lower bHLH39 expression in MET1-RNA interference lines compared with wild-type seedlings.Virus-induced gene silencing of bHLH39 and luciferase reporter assays revealed that bHLH39 is positively involved in the modulation of Fe homeostasis.Altogether,we propose that dynamic epigenetic DNA methylation in the CG context at the bHLH39 promoter is involved in its transcriptional regulation,thus contributing to the Fe deficiency response of tomato.

关键词： roots homeostasis treatment

Weighted Moore-Penrose Inverses and Weighted Core Inverses in Rings with Involution

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Chinese Annals of Mathematics,Series B 2021年第4期42卷 613-624页

作者： huihui zhu Qing-Wen WANG Department of Mathematics Shanghai UniversityShanghai 200444China School of Mathematics Hefei University of TechnologyHefei 230009China

In this paper,the authors derive the existence criteria and the formulae of the weighted Moore-Penrose inverse,the e-core inverse and the f-dual core inverse in rings.Also,new characterizations between weighted Moore-... 详细信息

关键词： Weighted Moore-Penrose inverses One-sided inverses along an element Inverses along an element e-Core inverses f-Dual core inverses

Soil-Transmitted Helminthiasis--China,2018

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China CDC weekly 2020年第3期2卷 34-38页

作者： huihui zhu Jilei Huang Changhai Zhou Tingjun zhu Menbao Qian Mizhen Zhang Yingdan Chen Shizhu Li National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention/Chinese Center for Tropical Diseases Research/WHO Collaborating Centre for Tropical Diseases/National Center for International Research on Tropical DiseasesMinistry of Science and Technology/Key Laboratory of Parasite and Vector BiologyMinistry of HealthShanghaiChina

What is already known about this topic?Soil-transmitted helminthiasis(STH)is distributed widely in China and a large number of the population is afflicted.However,trends of STH infections are decreasing.What is added by this report?The most recent data indicates that the overall prevalence of STH was 1.29% in 2018 in China,which was based on the national sentinel surveillance and demonstrates a continuous decline pattern.What are the implications for public health practice?Considering the current prevalence in China and various endemic statuses in different regions,precision control measures should be implemented for the control and elimination of STH in China.

关键词： What China. elimination

Doxorubicin-conjugated pH-responsive gold nanorods for combined photothermal therapy and chemotherapy of cancer

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Bioactive Materials 2018年第3期3卷 347-354页

作者： Jin Chen Xiao Li Xinlian Zhao QianQian Wu huihui zhu Zhengwei Mao Changyou Gao MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027PR China The Department of Gynecologic Oncology Women's HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310006China Women's Reproductive Health Laboratory of Zhejiang Province Women's HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang310006China

Cancer chemotherapy can be hindered by drug resistance which leads to lower drug efficiency.Here,we have developed a drug delivery system that tethers doxorubicin to the surface of gold nanorods via a pHsensitive linkage(AuNRs@DOX),for a combined photothermal and chemical therapy for cancer.First,AuNRs@DOX is ingested by HepG2 liver cancer cells.After endocytosis,the acidic pH triggers the release of doxorubicin,which leads to chemotherapeutic effects.The gold nanorods are not only carriers of DOX,but also photothermal conversion agents.In the presence of an 808 nm near-infrared laser,AuNRs@DOX significantly enhance the cytotoxicity of doxorubicin via the photothermal effect,which induces elevated apoptosis of hepG2 cancer cells,leading to better therapeutic effects in vitro and in vivo.

关键词： Gold nanorods Doxorubicin pH responsive Photothermal therapy Combinational therapy

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建筑工程施工管理与进度控制措施分析

工程建设（维泽科技） 2024年第4期7卷 35-37页

作者：朱慧慧浙江祥宏生态建设有限公司浙江宁波315602

由于经济的不断发展、城市化进程不断加快,使得建筑行业发展更加迅速的同时工程项目也越来越多,竞争变得十分激烈。人们将关注的重点放在了如何在确保建筑施工质量的基础上,尽可能地提高工程施工的进度。建筑工程项目要想最大限度地发... 详细信息

由于经济的不断发展、城市化进程不断加快,使得建筑行业发展更加迅速的同时工程项目也越来越多,竞争变得十分激烈。人们将关注的重点放在了如何在确保建筑施工质量的基础上,尽可能地提高工程施工的进度。建筑工程项目要想最大限度地发挥其经济效益,并提高其竞争力,前提必须在建筑工程项目约定的时间内高质量地完成。在实际的建筑项目管理过程中,进度控制是其基本的管理目标。为此,文中简要分析了建筑工程进度控制与管理的必要性,并重点分析了影响工程进度的因素以及相应的解决措施,希望能进一步优化建筑工程施工的进度,尽可能地做好对其的管理工作。

关键词：建筑工程施工管理进度控制有效措施

Antimony fluoride(SbF_(3)):A potent hole suppressor for tin(II)-halide perovskite devices

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InfoMat 2023年第1期5卷 106-113页

作者： Ao Liu huihui zhu Soonhyo Kim Youjin Reo Yong-Sung Kim Sai Bai Yong-Young Noh Department of Chemical Engineering Pohang University of Science and TechnologyPohangRepublic of Korea Korea Research Institute of Standards and Science DaejeonRepublic of Korea Department of Nano Science University of Science and TechnologyDaejeonRepublic of Korea Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengduPeople's Republic of China

Tin(Sn^(2+))-based halide perovskites have been developed as the most prom-ising alternatives to their toxic Pb-based counterparts in optoelectronic devices.However,the facile tin vacancy formation and easy oxidization characteristics make Sn^(2+)-based perovskites highly p-doped with excessive hole concentrations,which significantly hinder their applications.Herein,we demonstrate a potent hole inhibitor of antimony fluoride(SbF_(3)),which possesses a higher hole-suppression capability than conventional tin fluo-ride(SnF_(2)).A small amount of SbF_(3) allows a wide range of hole-density modulation with no or less SnF_(2) addition,thus mitigating the negative effects of using only SnF_(2).A SnF_(2)/SbF_(3) co-additive approach was further developed to achieve high-performance Sn 2+perovskite thin-film transis-tors operated in the enhancement mode with a five-fold enhancement of the field-effect mobility and improved operational stability compared to using only SnF_(2).We expect that the SbF 3 hole suppressor and co-additive approach can provide opportunities for the development of high-efficiency Sn^(2+)-perovskite optoelectronic devices.

关键词： electrical characterization hole suppressor thin-film transistor tin-based halide perovskite

Integrative proteomics reveals the role of E3 ubiquitin ligase SYVN1 in hepatocellular carcinoma metastasis

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Cancer Communications 2021年第10期41卷 1007-1023页

作者： Feiyang Ji Menghao Zhou Zeyu Sun Zhengyi Jiang huihui zhu Zhongyang Xie Xiaoxi Ouyang Lingjian Zhang Lanjuan Li State Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310003P.R.China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases HangzhouZhejiang 310003P.R.China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310003P.R.China

Background:Tumor metastasis is a major factor for poor prognosis of hepatocellular carcinoma(HCC),but the relationship between ubiquitination and metastasis need to be studiedmore systematically.We analyzed the ubiquitinome of HCC in this study to have a more comprehensive insight into human HCC metastasis.Methods:The protein ubiquitination levels in 15 HCC specimens with vascular invasion and 15 without vascular invasion were detected by ubiquitinome.Proteins with significantly different ubiquitination levels between HCCs with and without vascular invasion were used to predict E3 ubiquitin ligases associated with tumor metastasis.The topological network of protein substrates and corresponding E3 ubiquitin ligaseswas constructed to identify the key E3 ubiquitin ligase.Besides,the growth,migration and invasion ability of LM3 and HUH7 hepatoma cell lines with andwithout SYVN1 expression interferencewere measured by cell proliferation assay,subcutaneous tumor assay,umphal vein endothelium tube formation assay,transwell migration and invasion assays.Finally,the interacting proteins of SYVN1 were screened and verified by protein interaction omics,immunofluorescence,and immunoprecipitation.Ubiquitin levels of related protein substrates in LM3 and HUH7 cells were compared in negative control,SYVN1 knockdown,and SYVN1 overexpression groups.Results:In this study,our whole-cell proteomic dataset and ubiquitinomic dataset contained approximately 5600 proteins and 12,000 ubiquitinated sites.We discovered increased ubiquitinated sites with shorter ubiquitin chains during the progression ofHCC metastasis.In addition,proteomic and ubiquitinomic analyses revealed that high expression of E3 ubiquitin-protein ligase SYVN1 is related with tumor metastasis.Furthermore,we found that SYVN1 interacted with heat shock protein 90(HSP90)and impacted the ubiquitination of eukaryotic elongation factor 2 kinase(EEF2K).Conclusions:The ubiquitination profiles of HCC with and without vascular invasion were significantly different.SYVN1 was the

关键词： cancer E3 ubiquitin-protein ligase eukaryotic elongation factor 2 kinase heat shock protein 90 hepatocellular carcinoma liver metastasis proteomics synoviolin ubiquitin