CG hypermethylation of the bHLH39 promoter regulates its expression and Fe deficiency responses in tomato roots
作者机构:State Key Laboratory of Plant Physiology and BiochemistryCollege of Life SciencesZhejiang UniversityHangzhou 310058China Research Centre for Plant RNA SignalingCollege of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China Laboratory of Cell&Molecular BiologyInstitute of Vegetable ScienceZhejiang UniversityHangzhou 310058China
出 版 物:《Horticulture Research》 (园艺研究(英文))
年 卷 期:2023年第10卷第7期
页 面:125-137页
核心收录:
学科分类:0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学]
基 金:This work was supported financially by the Natural Science Foundation of Zhejiang Province(LZ22C150001) the China Postdoctoral Science Foundation(2019 M652064)
主 题:roots homeostasis treatment
摘 要:Iron(Fe)is an essential micronutrient for all organisms,including plants,whose limited bioavailability restricts plant growth,yield,and nutritional *** the transcriptional regulation of plant responses to Fe deficiency have been extensively studied,the contribution of epigenetic modulations,such as DNA methylation,remains poorly ***,we report that treatment with a DNA methylase inhibitor repressed Fe deficiency-induced responses in tomato(Solanum lycopersicum)roots,suggesting the importance of DNA methylation in regulating Fe deficiency *** changes in the DNA methylome in tomato roots responding to short-term(12 hours)and long-term(72 hours)Fe deficiency identified many differentially methylated regions(DMRs)and DMR-associated *** DMRs occurred at CHH sites under short-term Fe deficiency,whereas they were predominant at CG sites following long-term Fe ***,no correlation was detected between the changes in DNA methylation levels and the changes in transcript levels of the affected genes under either short-term or long-term ***,one exception was CG hypermethylation at the bHLH39 promoter,which was positively correlated with its transcriptional *** agreement,we detected lower CG methylation at the bHLH39 promoter and lower bHLH39 expression in MET1-RNA interference lines compared with wild-type ***-induced gene silencing of bHLH39 and luciferase reporter assays revealed that bHLH39 is positively involved in the modulation of Fe ***,we propose that dynamic epigenetic DNA methylation in the CG context at the bHLH39 promoter is involved in its transcriptional regulation,thus contributing to the Fe deficiency response of tomato.