CG hypermethylation of the bHLH39 promoter regulates its expression and Fe deficiency responses in tomato roots
作者机构：State Key Laboratory of Plant Physiology and BiochemistryCollege of Life SciencesZhejiang UniversityHangzhou 310058China Research Centre for Plant RNA SignalingCollege of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China Laboratory of Cell&Molecular BiologyInstitute of Vegetable ScienceZhejiang UniversityHangzhou 310058China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2023年第10卷第7期
页 面：125-137页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学]
基 金：This work was supported financially by the Natural Science Foundation of Zhejiang Province(LZ22C150001) the China Postdoctoral Science Foundation(2019 M652064)
主 题：roots homeostasis treatment
摘 要：Iron(Fe)is an essential micronutrient for all organisms,including plants,whose limited bioavailability restricts plant growth,yield,and nutritional *** the transcriptional regulation of plant responses to Fe deficiency have been extensively studied,the contribution of epigenetic modulations,such as DNA methylation,remains poorly ***,we report that treatment with a DNA methylase inhibitor repressed Fe deficiency-induced responses in tomato(Solanum lycopersicum)roots,suggesting the importance of DNA methylation in regulating Fe deficiency *** changes in the DNA methylome in tomato roots responding to short-term(12 hours)and long-term(72 hours)Fe deficiency identified many differentially methylated regions(DMRs)and DMR-associated *** DMRs occurred at CHH sites under short-term Fe deficiency,whereas they were predominant at CG sites following long-term Fe ***,no correlation was detected between the changes in DNA methylation levels and the changes in transcript levels of the affected genes under either short-term or long-term ***,one exception was CG hypermethylation at the bHLH39 promoter,which was positively correlated with its transcriptional *** agreement,we detected lower CG methylation at the bHLH39 promoter and lower bHLH39 expression in MET1-RNA interference lines compared with wild-type ***-induced gene silencing of bHLH39 and luciferase reporter assays revealed that bHLH39 is positively involved in the modulation of Fe ***,we propose that dynamic epigenetic DNA methylation in the CG context at the bHLH39 promoter is involved in its transcriptional regulation,thus contributing to the Fe deficiency response of tomato.
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