The miR157-SPL-CNR module acts upstream of bHLH101 to negatively regulate iron deficiency responses in tomato

作     者：Huihui Zhu Jiayi Wang Dan Jiang Yiguo Hong Jiming Xu Shaojian Zheng Jianli Yang Weiwei Chen Huihui Zhu;Jiayi Wang;Dan Jiang;Yiguo Hong;Jiming Xu;Shaojian Zheng;Jianli Yang;Weiwei Chen

作者机构：State Key Laboratory of Plant Physiology and BiochemistryCollege of Life SciencesZhejiang UniversityHangzhou 310058China Research Centre for Plant RNA SignalingCollege of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 311121China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第5期

页      面：1059-1075页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：financially supported by grants from the Natural Science Foundation of Zhejiang Province(LZ22C150001) China Postdoctoral Science Foundation(2019M652064) China Scholarship Council(3035) 

主　　题：Fe deficiency miR157 SPL-CNR tomato transcriptional repressor 

摘      要：Iron(Fe)homeostasis is critical for plant growth,development,and stress *** levels are tightly controlled by intricate regulatory networks in which transcription factors(TFs)play a central role.A series of basic helix-loop-helix(b HLH)TFs have been shown to contribute to Fe homeostasis,but the regulatory layers beyond b HLH TFs remain largely ***,we demonstrate that the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE(SPL)TF Sl SPL-CNR negatively regulates Fe-deficiency responses in tomato(Solanum lycopersicum)*** deficiency rapidly repressed the expression of Sl SPL-CNR,and Fe deficiency responses were intensified in two clustered regularly interspaced palindromic repeats(CRISPR)/CRISPRassociated protein 9-generated Sl SPL-CNR knockout lines compared to the *** transcriptome analysis identified 47 Fe deficiencyresponsive genes the expression of which is negatively regulated by Sl SPL-CNR,one of which,Slb HLH101,helps regulate Fe uptake *** SPLCNR localizes the nucleus and interacts with the GTAC and BOX 4(ATTAAT)motifs in the Slb HLH101 promoter to repress its *** of Sl SPL-CNR expression in response to Fe deficiency was well correlated with the expression of the micro RNA Slymi *** R157-overexpressing tomato lines displayed enhanced Fe deficiency responses,as did Sl SPL-CNR loss-of-function *** propose that the Slymi R157-Sl SPL-CNR module represents a novel pathway that acts upstream of Slb HLH101 to regulate Fe homeostasis in tomato roots.