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MiR-301a promotes embryonic stem cell differentiation to cardiomyocytes

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World Journal of Stem Cells 2019年第12期11卷 1130-1141页

作者： Li-Xiao Zhen Yu-Ying Gu Qian Zhao hui-fang zhu Jin-hui Lv Shu-Jun Li Zhen Xu Li Li Zuo-Ren Yu Key Laboratory of Arrhythmias of the Ministry of Education of China Tongji University School of MedicineShanghai 200120China Research Center for Translational Medicine Shanghai East HospitalTongji University School of MedicineShanghai 200120China Department of Microbiology and Immunology Wenzhou Medical CollegeWenzhou 325000Zhejiang ProvinceChina

BACKGROUND Cardiovascular disease is the leading cause of death *** repair after pathological injury in the heart remains a major challenge due to the limited regenerative ability of cardiomyocytes in *** cell-derived cardiomyocytes provide a promising source for the cell transplantation-based treatment of injured *** To explore the function and mechanisms of miR-301a in regulating cardiomyocyte differentiation of mouse embryonic stem(mES)cells,and provide experimental evidence for applying miR-301a to the cardiomyocyte differentiation induction from stem *** mES cells with or without overexpression of miR-301a were applied for all functional *** hanging drop technique was applied to form embryoid bodies from mES *** markers including GATA-4,TBX5,MEF2C,andα-actinin were used to determine cardiomyocyte differentiation from mES *** High expression of miR-301a was detected in the heart from late embryonic to neonatal *** of miR-301a in mES cells significantly induced the expression of cardiac transcription factors,thereby promoting cardiomyocyte differentiation and beating cardiomyocyte clone *** is a target gene of miR-301a in ***-regulated PI3K-AKT-mTOR-Stat3 signaling showed involvement in regulating miR-301a-promoted cardiomyocyte differentiation from mES *** MiR-301a is capable of promoting embryonic stem cell differentiation to cardiomyocytes.

关键词： miR-301a Mouse embryonic stem cells Differentiation Cardiomyocytes

Natural products sensitize tumor cell to recognition and lysis by NK cell

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Natural products sensitize tumor cell to recognition and lys...

Expression pattern of matrix metalloproteinases-13 in a rat model of alcoholic liver fibrosis

中国化学会第十二届全国天然有机化学学术会议

作者： hui-fang zhu 李艳 State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of Sciences

Natural killer(NK) cell-mediated immunotherapy is an attractive approach to treat cancers .Natural products from plants,animals,microbes,and halobios are precious treasure and contains numerous bioactive compounds to cure *** on the abundant resources pool in southwest China,a screening effort was initiated to identify novel lead compounds sensitizing tumor cells to NK cell *** a result,potential natural products were obtained which significantly stimulated the expression of NKG2 D ligands on tumor cells and greatly sensitized their recognition and lysis by NK *** regulatory mechanism and the in vivo anti-cancer activity was evaluated,which is important for the discovery of new agents for NK cell-based immunotherapy.

关键词： natural products NK cell NKG2D ligands immunotherapy

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Hepatobiliary & Pancreatic Diseases International 2005年第4期4卷 569-572页

作者： Sheng Yan, Ge-Min Chen, Chao-hui Yu, Guo-fang zhu, You-Ming Li and Shu-Sen Zheng Departments of Hepatobiliary Surgery and Gastroenterology First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Department of Hepatobiliary Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China. yansheng@***

BACKGROUND: Interstitial collagenase has been considered as an essential enzyme for collagenolysis in liver fi-brosis, because type Ⅰ and Ⅲ collagens increase predominantly in liver fibrosis. The present study aimed to demonstrate the gene expression of matrix metalloproteinases-13 (MMP-13) in the progressive phases of ethanol induced experimental liver fibrosis in rats. METHODS: Thirty-four Sprague-Dawley rats were randomly divided into two groups. The experimental group (24 rats) was given ethanol (44% , 7 g/kg) every day and the control group (10) was given normal saline. Liver samples were harvested from experimental rats at 4, 12 and 24 weeks respectively. The kinetics of MMP-13 mRNA expression was assayed by semi-quantity reverse transcriptase-poly-merase chain reaction (RT-PCR). RESULTS: In normal rat liver, a faint band for MMP-13 mRNA was observed by RT-PCR (0.24±0.41). The gene expression of MMP-13 was increased in the liver of the rats treated with ethanol for 4 weeks (0. 62 ±0. 54), but it was not considered statistically significant (P >0.05). And the livers from 12-week-treated rats showed a marked mRNA expression(1.65 ±0.47, P <0. 01). Once fibrosis became prominent (24 weeks), a faint band of MMP-13 mRNA was observed (0.39±0.25). CONCLUSION: MMP-13 participates in the degradation of newly-formed matrix in the early phase of rat liver fibrosis induced by ethanol, and it was induced in a distinct time frame.

关键词： matrix metalloproteinases-13 liver fibrosis reverse transcriptase-polymerase chain reaction alcohol

CagA+ H pylori infection is associated with polarization of T helper cell immune responses in gastric carcinogenesis

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World Journal of Gastroenterology 2007年第21期13卷 2923-2931页

作者： Shu-Kui Wang hui-fang zhu Bang-Shun He Zhen-Yu Zhang Zhi-Tan Chen Zi-Zheng Wang Guan-Ling Wu Department of Pathogen Biology Nanjing Medical University Nanjing 210029 Jiangsu Province China Affiliated Nanjing First Hospital Nanjing Medical University Nanjing 210006 Jiangsu Province China College of Life Sciences Nanjing Normal University Nanjing 210097 Jiangsu Province China

AIM:To characterize the immune responses including local and systemic immunity induced by infection with H pylori,especially with CagA+ H pylori strains and the underlying immunopathogenesis. METHODS:A total of 711 patients with different gastric lesions were recruited to determine the presence of H pylori infection and cytotoxin associated protein A (CagA),the presence of T helper (Th) cells and regulatory T (Treg) cells in peripheral blood mononuclear cells (PBMCs),expression of plasma cytokines,and RNA and protein expression of IFN-γ and IL-4 in gastric biopsies and PBMCs were determined by rapid urease test,urea 14C breath test,immunoblotting test,flow cytometry ,real time RT-PCR and immunohistochemistry. RESULTS:Of the patients,629 (88.47%) were infected with H pylori ; 506 (71.16%) with CagA+ and 123 (17.30%) with CagA- strains. Among patients infected with CagA+ H pylori strains,Th1-mediated cellular immunity was associated with earlier stages of gastric carcinogenesis,while Th2-mediated humoral immunity dominated the advanced stages and was negatively associated with an abundance of Treg cells. However,there was no such tendency in Th1/Th2 polarization in patients infected with CagA- H pylori strains and those without H pylori infection. CONCLUSION:Polarization of Th cell immune responses occurs in patients with CagA+ H pyloriinfection,which is associated with the stage and severity of gastric pathology during the progression of gastric carcinogenesis. This finding provides further evidence for a causal role of CagA+ H pylori infection in the immunopathogenesis of gastric cancer.

关键词： H pylori CagA Gastric carcinogenesis Thelper cells Regulatory T cells Immune response

Anomalous Metallic State Driven by Magnetic Field at the LaAlO_(3)/KTaO_(3)(111)Interface

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Chinese Physics Letters 2023年第3期40卷 36-40页

作者：张子涛乔宇杰邵婷娜赵强陈星宇陈美慧朱芳慧窦瑞芬刘海文熊昌民聂家财 Department of Physics Beijing Normal UniversityBeijing 100875China

The origin of the quantum superconductor to metal transition at zero temperature in two-dimensional superconductors is still an open problem,which has caused intensely ***,we report the observation of a quantum superconductor-to-metal transition in La Al O_(3)/KTa O_(3)(111)interface,driven by magnetic *** a small magnetic field perpendicular to the film plane is applied,the residual saturated resistance is observed,indicating the emergence of an anomalous metallic state associated with a failed *** dependence of saturated resistance on magnetic field at low temperature indicates that the observed metal state is a Bose metal *** our findings,magnetic field regulating La Al O_(3)/KTa O_(3)(111)interface emerges as a platform to scrutinize the details of the anomalous metallic state in a controllable way.

关键词： resistance saturated superconductor

Nitrogen addition promotes soil carbon accumulation globally

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Science China Life Sciences 2025年第1期 284-293页

作者： Xuemei Yang Suhui Ma Erhan Huang Danhua Zhang Guoping Chen Jiangling zhu Chengjun Ji Biao zhu Lingli Liu Jingyun fang Institute of Ecology College of Urban and Environmental Sciencesand Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking University Ministry of Education Key Laboratory for Transboundary Ecosecurity of Southwest China School of Ecology and Environmental ScienceYunnan University State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of Sciences

Soil is the largest carbon(C) reservoir in terrestrial ecosystems and plays a crucial role in regulating the global C cycle and climate *** nitrogen(N) deposition has been widely considered as a critical factor affecting soil organic carbon(SOC) storage, but its effect on SOC components with different stability remains unclear. Here, we analyzed extensive empirical data from 304 sites worldwide to investigate how SOC and its components respond to N addition. Our analysis showed that N addition led to a significant increase in bulk SOC(6.7%), with greater increases in croplands(10.6%) and forests(6.0%) compared to grasslands(2.1%). Regarding SOC components, N addition promoted the accumulation of plant-derived C(9.7%–28.5%) over microbial-derived C(0.2%), as well as labile(5.7%) over recalcitrant components(–1.2%), resulting in a shift towards increased accumulation of plant-derived labile C. Consistently, N addition led to a greater increase in particulate organic C(11.9%) than mineral-associated organic C(3.6%), suggesting that N addition promotes C accumulation across all pools, with more increase in unstable than stable pools. The responses of SOC and its components were best predicted by the N addition rate and net primary productivity. Overall, our findings suggest that N enrichment could promote the accumulation of plant-derived and non-mineral associated C and a subsequent decrease in the overall stability of soil C pool, which underscores the importance of considering the effects of N enrichment on SOC components for a better understanding of C dynamics in soils.

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国家治理的社会创新:健全人口发展支持和服务体系的治理现代化

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河北学刊 2025年第1期45卷 140-151页

作者：朱荟南开大学社会学院社会工作与社会政策系

在进一步全面深化改革的时代背景下,探讨社会创新在健全人口发展支持和服务体系中的作用显得尤为关键。由于人口新国情与治理现代化之间的限度,中国人口治理的社会创新始终面临一系列挑战。中华人民共和国成立以来,中国人口治理的创新... 详细信息

在进一步全面深化改革的时代背景下,探讨社会创新在健全人口发展支持和服务体系中的作用显得尤为关键。由于人口新国情与治理现代化之间的限度,中国人口治理的社会创新始终面临一系列挑战。中华人民共和国成立以来,中国人口治理的创新实践从工具主义导向的偶发性举措创新,到市场驱动下的政策形式创新,再到风险叠加背景下的工具理性创新,最终发展至人口国情下的实质性社会创新。通过构建一个涵盖“横向协调、纵向协同、长远规划”的三维模型,重新建立健全人口发展支撑和服务体系的理论解释框架。该框架的主旨内涵是国家治理的社会创新,要求从整体视角审视人口治理的现代化进程,促进国家战略转向为社会行动。即健全人口发展支持和服务体系在未来实践中,将进一步拓展为“发展性—社会包容”“普惠性—社会投资”“基础性—社会动员”“兜底性—社会保护”的治理现代化矩阵。

关键词：人口发展支持和服务体系全面深化改革国家治理社会创新

黄土区小流域植被/工程复合作用下的泥沙效应

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水生态学杂志 2017年第5期38卷 7-13页

作者：刘卉芳朱毕生解刚王昭艳中国水利水电科学研究院北京100038

研究植被/工程复合作用下的泥沙效应,为科学阐述黄土区不同水土保持措施耦合作用下的调水调沙效益提供参考依据。马家沟流域土地利用主要分为草地、林地、梯田、坡耕地、灌木林地、裸地、居民地和水域8类,流域布设各类淤地坝64座。基于S... 详细信息

研究植被/工程复合作用下的泥沙效应,为科学阐述黄土区不同水土保持措施耦合作用下的调水调沙效益提供参考依据。马家沟流域土地利用主要分为草地、林地、梯田、坡耕地、灌木林地、裸地、居民地和水域8类,流域布设各类淤地坝64座。基于SWAT模型,对比分析了多林流域和少林流域对流域泥沙的调节作用,分析了淤地坝淤积库容、单坝拦沙效益,研究了流域各项水土保持措施减沙效益。研究结果表明,森林植被除了缓洪增枯,同时可以减少流域侵蚀产沙,农果地与全林流域相比,输沙模数为后者的1.5倍;淤地坝坝控面积与坝高、库容与坝高均正相关,相关系数分别为R2=0.8121、0.8603。黄土区流域植被/工程复合作用下水土保持效果显著,而在各项水土保持措施中,淤地坝减沙贡献率最大,达40%以上。

关键词：黄土区植被/工程泥沙效应

中国的资本账户开放、汇率制度改革与货币危机风险

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国际贸易问题 2016年第11期 165-176页

作者：程惠芳朱一鸣潘奇姚遥浙江工业大学经贸管理学院杭州师范大学国际服务工程学院 311121

在国内金融开放改革面临现实抉择之际,本文采用1970-2011年的102个国家面板数据,构建Logistic计量模型,实证分析中国的资本账户开放程度和汇率制度选择对于货币危机风险的现实影响。实证研究发现,随着资本账户开放程度的提高,选择实施... 详细信息

在国内金融开放改革面临现实抉择之际,本文采用1970-2011年的102个国家面板数据,构建Logistic计量模型,实证分析中国的资本账户开放程度和汇率制度选择对于货币危机风险的现实影响。实证研究发现,随着资本账户开放程度的提高,选择实施浮动汇率制度或超级固定汇率制有助于降低金融危机风险。利用以上模型,结合中国宏观经济数据可以测算出,在中国当前金融开放改革中,保持有限资本开放,率先实施汇率制度灵活性改革,有利于降低货币危机风险。本研究结论是中国的金融开放改革为优化决策提供了重要的理论依据和实践参考。

关键词：资本账户开放汇率制度货币危机