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Expression pattern of matrix metalloproteinases-13 in a rat model of alcoholic liver fibrosis

Expression pattern of matrix metalloproteinases-13 in a rat model of alcoholic liver fibrosis

作     者:Sheng Yan, Ge-Min Chen, Chao-Hui Yu, Guo-Fang Zhu, You-Ming Li and Shu-Sen Zheng Departments of Hepatobiliary Surgery and Gastroenterology First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China 

作者机构:Department of Hepatobiliary Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China. yansheng@*** 

出 版 物:《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志(英文版))

年 卷 期:2005年第4卷第4期

页      面:569-572页

学科分类:1002[医学-临床医学] 100201[医学-内科学(含:心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主  题:matrix metalloproteinases-13 liver fibrosis reverse transcriptase-polymerase chain reaction alcohol 

摘      要:BACKGROUND: Interstitial collagenase has been considered as an essential enzyme for collagenolysis in liver fi-brosis, because type Ⅰ and Ⅲ collagens increase predominantly in liver fibrosis. The present study aimed to demonstrate the gene expression of matrix metalloproteinases-13 (MMP-13) in the progressive phases of ethanol induced experimental liver fibrosis in rats. METHODS: Thirty-four Sprague-Dawley rats were randomly divided into two groups. The experimental group (24 rats) was given ethanol (44% , 7 g/kg) every day and the control group (10) was given normal saline. Liver samples were harvested from experimental rats at 4, 12 and 24 weeks respectively. The kinetics of MMP-13 mRNA expression was assayed by semi-quantity reverse transcriptase-poly-merase chain reaction (RT-PCR). RESULTS: In normal rat liver, a faint band for MMP-13 mRNA was observed by RT-PCR (0.24±0.41). The gene expression of MMP-13 was increased in the liver of the rats treated with ethanol for 4 weeks (0. 62 ±0. 54), but it was not considered statistically significant (P 0.05). And the livers from 12-week-treated rats showed a marked mRNA expression(1.65 ±0.47, P 0. 01). Once fibrosis became prominent (24 weeks), a faint band of MMP-13 mRNA was observed (0.39±0.25). CONCLUSION: MMP-13 participates in the degradation of newly-formed matrix in the early phase of rat liver fibrosis induced by ethanol, and it was induced in a distinct time frame.

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