检索结果-南通市图书馆

Interaction between enteric epithelial cells and Peyer's patch lymphocytes in response to Shigella lipopolysaccharide: Effect on nitric oxide and IL-6 release

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第24期12卷 3895-3900页

作者： Jie Chen Chuen-Pei Ng Dewi K Rowlands Peng-Hui Xu Jie-Ying Gao Yiu-Wa Chung hsiao-chang chan Epithelial Cell Biology Research Center Department of Physiology Faculty of Medicine Chinese University of Hong Kong Hong Kong China Department of Immunology Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 China Department of Biology Faculty of Medicine ShanxiMedical University Taiyuan 030001 Shanxi Province China

AIM： TO investigate the effect of interaction between enteric epithelial cells and lymphocytes of Peyer＇s patch on the release of nitric oxide （NO） and IL-6 in response to Shigella lipopolysaccharide （LPS）. METHODS： Human colonic epithelial cells （Caco-2） were mixed cocultured with lymphocytes of Peyer＇s patch from wild-type （C57 mice） and inducible NO synthase knockout mice, and challenged with Shigella F2a-12 LPS. Release of NO and raiL-6 was measured by Griess colorimetric assay and enzyme-linked immunosorbent assay （ELISA）, respectively. RESULTS： In the absence of LPS challenge, NO was detected in the culture medium of Caco-2 epithelial cells but not in lymphocytes of Peyer＇s patch, and the NO release was further up-regulated in both cocultures with lymphocytes from either the wild-type or iNOS knockout mice, with a significantly higher level observed in the coculture with iNOS knockout lymphocytes. After Shigella F2a-12 LPS challenge for 24-h, NO production was significantly increased in both Caco-2 alone and the coculture with lymphocytes of Peyer＇s patch from the wild-type mice but not from iNOS knockout mice. LPS was found to stimulate the release of mIL-6 from lymphocytes, which was suppressed by coculture with Caco-2 epithelial cells. The LPS-induced mIL-6 production in lymphocytes from iNOS knockout mice was significantly greater than that from the wild-type mice. CONCLUSION： Lymphocytes of Peyer＇s patch maintain a constitutive basal level of NO production from the enteric epithelial cell Caco-2. LPS-induced mIL-6 release from lymphocytes of Peyer＇s patch is suppressed by the cocultured epithelial cells. While no changes are detectable in NO production in lymphocytes from both wild-type and iNOS knockout mice before and after LPS challenge, NO from lymphocytes appears to play an inhibitory role in epithelial NO release and their own mIL-6 release in response to LPS.

关键词： Shigella F2a-12 LPS Colon epithelial cells(Caco-2) Peyer's patch lymphocyte Coculture Nitricoxide Interleukin-6

Critical Role of Cystic Fibrosis Transmembrane Conductance Regulation(CFTR)in Female Reproduction

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

皖南医学院学报 2003年第Z1期22卷 4-5页

作者： hsiao chang chan Epithelial Cell Biology Research Center Department of PhysiologyFaculty of MedicineThe Chinese University of Hong KongShatinHong Kong

　　Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated Cl- channel, mutations of which are responsible for defective Cl- and/or HCO-3 secretions seen in cystic fibrosis (CF), a common lethal genetic disease affecting most exocrine glands/organs, including the lungs, intestine, pancreas and reproductive tracts of both sexes.　　……

关键词： cystic fibrosis genetic disease

Tetramethylpyrazine stimulates cystic fibrosis transmembrane conductance regulator-mediated anion secretion in distal colon of rodents

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第27期11卷 4173-4179页

作者： Qiong He Jin-Xia Zhu Ying Xing Lai-Ling Tsang Ning Yang Dewi Kenneth Rowlands Yiu-Wa Chung hsiao-chang chan Department of Physiology Medical School Zhengzhou University Zhengzhou 450052 Henan Province China Epithelial Cell Biology Research Center Department of PhysiologyFaculty of Medicine The Chinese University of Hong Kong ShatinHong Kong China

AIM： To investigate the effect of tetramethylpyrazine （TMP）, an active compound from Ligustium Wollichii Franchat, on electrolyte transport across the distal colon of rodents and the mechanism ***： The short-circuit current （Isc） technique in conjunction with pharmacological agents and specific inhibitors were used in analyzing the electrolyte transport across the distal colon of rodents. The underlying cellular signaling mechanism was investigated by radioimmunoassay analysis （RIA） and a special mouse model of cystic ***： IMP stimulated a conoentration-dependent rise in ISCl, which was dependent on both Cl^- and HCO3^-, and inhibited by apical application of diphenylamine-2,2＇-dicarboxylic acid （DPC） and glibenclamide, but resistant to 4,4＇-diisothiocyanatostilbene-2,2＇-disulfonic acid disodium salt hydrate （DIDS）. Removal of Na^＋ from basolateral solution almost completely abolished the Isc response to TMP, but it was insensitive to apical Na^＋ replacement or apical Na^＋ channel blocker, amiloride. Pretreatment of colonic mucosa with BAPTA-AM, a membrane-permeable selective Ca2＋ chelator, did not significantly alter the TMP-induced Iso No additive effect of forskolin and 3-isobutyl-l-methylxanthine （[BMX） was observed on the TMP-induced Isc, but it was significantly reduced by a protein kinase A inhibitor, *** results showed that TMP （1 mmol/L） elicited a significant increase in cellular cAMP production, which was similar to that elicited by the adenylate cyclase activator, forskolin （10μmol/L）. The TMP-elicited Isc as well as forskolin- or IBMX-induced Isc were abolished in mice with homozygous mutation of the cystic fibrosis transmembrane conductance regulator （CFTR） presenting defective CFTR functions and ***： TMP may stimulate cAMP-dependent and CFTR-mediated Cl^- and HCO3^- secretion. This may have implications in the future development of alternative treatment for constipation.

关键词： Adrenergic beta-Agonists Animals Anions Colon Constipation Cystic Fibrosis Transmembrane Conductance Regulator Male Mice Mice, Inbred CFTR Pyrazines Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't

The effect of the habenular nucleus lesion on the sleep-wake cycle

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The effect of the habenular nucleus lesion on the sleep-wake...

NYD-SP27,a novel intrinsic decapacitation factor in sperm

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： hsiao-chang chan Epithelial Cell Biology Research Center The Chinese University of Hong KongHong KongChina

＜正＞The neuroanatomy and neurophysiology studies have reported that the habenular nucleus（Hb） has extensive interconnections with many brain areas related to the sleep-wake cycle,especially the hypothalamus,and thus it may play an important regulatory role in the sleep-wake *** order to understand how the Hb modulates the process,we first examined the expression profile of 123 genes in the hypothalamus after Hb lesion by using Neurotrophin and Receptors Oligo GEArray and followed by confirmation of the expression of the genes well-documented to be associated with sleep-wake cycle in the hypothalamus of Hb-lesioned rats by using *** Electroencephalographic（EEG） response to Hb-lesioned rats was also examined in order to further verify the effect of Hb lesion on the sleep-wake *** microarray results showed that there were 12 genes（approximately 10%of the genes examined） were differentially upregulated expression in the hypothalamus after Hb lesion,which could be grouped into waking-related *** subsequent RT-PCR results showed that tryptophan hydroxylase（TPH） and 5-HT-2A,5-HT-2B receptors expression was upregulated in the hypothalamus of Hb-lesioned rats compared to the sham-operated *** the EEG experiments,we found that the duration of REMS（dREMS） and the duration of NREMS（dNREM） was reduced and the duration of wakefulness（dWAKE） was increased in Hb-lesion group as compared to Hb-sham group,as well as in LHb-lesion group as compared to LHb-sham ***,there were no significant differences between groups in all measurements except for the decrease of dREMS and the increase of dWAKE between LHb-lesioned rats and LHb sham-operated *** together,the gene expression profiles and functional studies indicate that Hb,especially LHb,participated in the regulation of sleep and wakefulness process.

关键词： sleep-wake cycle habenular nucleus hypothalamus 5-HT EEG

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

NYD-SP27,a novel intrinsic decapacitation factor in sperm

Regulation of smooth muscle contractility by the epithelium in rat vas deferens:role of ATP-induced release of PGE2

第一届中华医学会生殖医学分会、中国动物学会生殖生物学分会联合年会

作者： Hau Yah Wong hsiao chang chan Epithelial Cell Biology Research Center Li Ka Shing Institute of Health SciencesDepartment of PhysiologyFaculty of MedicineThe Chinese University of Hong Kong.

Prior to fertilization sperm have to undergo an activation process known as capaciation, leading to the acrosome reaction which releases hydrolytic enzymes to facilitate sperm penetration of the ***,premature capacitation may lead to untimely release of sperm acrosomal enzymes before they reach the *** is known about the mechanism for preventing premature capacitation in sperm although decapacitation factors from various sources have been thought to be *** we report that NYD-SP27,an isoform of phospholipase C（PLC）ζ,previously shown to exert an inhibitory effect on the PLC-coupled Camobilization in the pancreas,is localized to sperm acrosome and shown to be detached from sperm as they undergo capacitation and acrosome *** absence of HCO,a key factor in activating capacitation,from the capacitation-inducing medium prevents the lost of NYD-SP27 from sperm. The antibody against NYD-SP27 also prevents the lost of NYD-SP27 from sperm and significantly reduces the number of capacitated sperm and aerosome reaction induced by ATP and progesterone,as well as inhibits the agonist-indueed PLC-coupled Camobilization in sperm,which can be mimicked by an inhibitor of PLC,*** data strongly suggest that NYD-SP27 is a physiological inhibitor of PLC that acts as an intrinsic decapacitation factor in sperm to prevent premature capacitation and acrosome reaction.

关键词： NYD-SP27 PLCζ sperm decapacitation factor capacitation acrosome reaction

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Regulation of smooth muscle contractility by the epithelium ...

The National Natural Science Foundation of China and the Epithelial Cell Biology Research Center

中南地区第八届生理学学术大会

作者： Hau YinWong YiuWa Chung hsiao chang chan Epithelial Cell Biology Research Center Li Ka Shing Institute of Health Sciences Department of Physiology Faculty of MedicineChinese University of Hong Kong Shatin NT Hong Kong China

＜正＞Recent studies suggest that the epithelium might modulate the contractility of smooth muscle. However, the mechanisms underlying this regulation are unknown. The present study investigated the regulation of smoo...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science Foundation in China 2002年第1期10卷 59-60页

作者： hsiao chang chan(Epithelial Cell Biology Research Center, The Chinese University of HongKong, Shatin, HongKong)

Although it has been only a couple of years since the establishment of the Epithelial Cell Biology Research Center, the first of its kind ever founded in China , the planning for the center establishment and the invol... 详细信息

关键词： Cell Re NSFC The National Natural Science Foundation of China and the Epithelial Cell Biology Research Center

An alternative splicing variant of mineralocorticoid receptor discovered in preeclampsia tissues and its effect on endothelial dysfunction

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2020年第3期63卷 388-400页

作者： Mengxi Guo Chengliang Zhou Gufeng Xu Lin Tang Yechun Ruan Ying Yu Xianhua Lin Dandan Wu Hao Chen Priscilla Yu Luyang Jin Yinyu Wang Yimei Wu Kamran Ullah Tanzil Ur Rahman Xinmei Liu Jianzhong Sheng hsiao-chang chan Hefeng Huang Women’s Hospital Zhejiang University School of MedicineHangzhou 310058China International Peace Maternal and Child Health Hospital Shanghai Jiao Tong University School of MedicineShanghai 200030China Epithelial Cell Biology Research Center Faculty of MedicineSchool of Biomedical SciencesThe Chinese University of Hong KongHong KongChina Key Laboratory of Reproductive Genetics Ministry of Education(Zhejiang University)Hangzhou 310058China Department of Pathology and Pathophysiology Zhejiang University School of MedicineHangzhou 310058China Shanghai Key Laboratory of Embryo Original Disease Shanghai 20030China

The pathophysiology of preeclampsia(PE)remains *** spiral artery remodeling dysfunction and PE offspring cardiovascular future development has been a worldwide *** collected placental and umbilical artery samples from normotensive and PE *** receptor(MR)and its alternative splicing variant(ASV)expression and their biological effects on PE were *** MR ASV was found to be highly expressed in all PE samples and slightly expressed in about half of the normotensive samples(umbilical artery,~57.58%;placenta,~36.84%).The MR ASV expression was positively associated with blood pressure in both *** MR ASV protein changed the aldosterone-induced expression pattern of MR target genes related to ion exchanges and cell signaling *** MR ASV can also impair the proliferation,migration,and tube formation ability of endothelial *** findings indicate that MR ASV in PE placenta plays a pathogenic role in PE pathophysiology,especially in endothelial dysfunction,and the existence of the MR ASV in PE umbilical artery provides a new direction in the study of PE offspring with increased risk of cardiovascular diseases.

关键词： preeclampsia mineralocorticoid receptor alternative splicing variant offspring endothelial dysfunction

Magnesium implantation or supplementation ameliorates bone disorder in CFTR-mutant mice through an ATF4-dependent Wnt/β-catenin signaling

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第2期7卷 95-108页

作者： Jiankun Xu Peijie Hu Xiaotian Zhang Junjiang Chen Jiali Wang Jieting Zhang Ziyi Chen Mei Kuen Yu Yiu Wa Chung Yan Wang Xiaohu Zhang Yifeng Zhang Nianye Zheng Hao Yao Jiang Yue hsiao chang chan Ling Qin Ye Chun Ruan Musculoskeletal Research Laboratory Department of Orthopedics&TraumatologyThe Chinese University of Hong KongHong KongChina Deparment of Biomedical Engineering Faculty of EngineeringThe Hong Kong Polytechnic UniversityHong KongChina Epithelial Cell Biology Research Centre School of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong KongChina School of Biomedical Engineering Sun Yat-sen UniversityGuangzhouChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina

Magnesium metal and its alloys are being developed as effective orthopedic implants;however,the mechanisms underlying the actions of magnesium on bones remain *** fibrosis,the most common genetic disease in Caucasians caused by the mutation of CFTR,has shown bone disorder as a key clinical manifestation,which currently lacks effective therapeutic *** we report that implantation of magnesium-containing implant stimulates bone formation and improves bone fracture healing in CFTR-mutant ***/β-catenin signaling in the bone is enhanced by the magnesium implant,and inhibition of Wnt/β-catenin by iCRT14 blocks the magnesium implant to improve fracture healing in CFTR-mutant *** further demonstrate that magnesium ion enters osteocytes,increases intracellular cAMP level and activates ATF4,a key transcription factor known to regulate Wnt/β-catenin *** vivo knockdown of ATF4 abolishes the magnesium implant-activated β-catenin in bones and reverses the improved-fracture healing in CFTR-mutant *** addition,oral supplementation of magnesium activates ATF4 and β-catenin as well as enhances bone volume and density in CFTR-mutant ***,these results show that magnesium implantation or supplementation may serve as a potential anabolic therapy for cystic fibrosis-related bone *** of ATF4-dependent Wnt/β-catenin signaling in osteocytes is identified as a previously undefined mechanism underlying the beneficial effect of magnesium on bone formation.

关键词： Magnesium implant Cystic fibrosis-related bone disorder ATF4 Wnt/β-catenin signaling