检索结果-南通市图书馆

High mobility group box 1 mediates inflammatory response of astrocytes via cyclooxygenase 2/prostaglandin E2 signaling following spinal cord injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第9期16卷 1848-1855页

作者： hong-hua song Tian-Cheng song Ting Yang Chun-Shuai Sun Bing-Qiang He Hui Li Ying-Jie Wang Yu Li Hao Wu Yu-Ming Hu Yong-Jun Wang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Center of Special Inspection Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Otolaryngology Head Neck Surgery Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

High mobility group box 1(HMGB1) interacts with pattern-recognition receptors of immune cells to activate the inflammatory response. Astrocytes play a positive role in the inflammatory response of the central nervous system by expressing a broad range of patternrecognition receptors. However, the underlying relationship between HMGB1 and the inflammatory reaction of astrocytes remains unclear. In this study, we established rat models of spinal cord injury via laminectomy at the T8–10 level, and the injured spinal cord was subjected to transcriptome sequencing. Our results showed that the HMGB1/Toll-like receptor 4(TLR4) axis was involved in the activation of astrocyte inflammatory response through regulation of cyclooxygenase 2(COX2)/prostaglandin E2(PGE2) signaling. Both TLR4 and COX2 were distributed in astrocytes and showed elevated protein levels following spinal cord injury. Stimulation of primary astrocytes with recombinant HMGB1 showed that COX2 and microsomal PGE synthase(mPGES)-1, rather than COX1, mPGES-2, or cytosolic PGE synthase, were significantly upregulated. Accordingly, PGE2 production in astrocytes was remarkably increased in response to recombinant HMGB1 challenges. Pharmacologic blockade of TLR2/4 attenuated HMGB1-mediated activation of the COX2/PGE2 pathway. Interestingly, HMGB1 did not impact the production of tumor necrosis factor-α or interleukin-1β in astrocytes. Our results suggest that HMGB1 mediates the astrocyte inflammatory response through regulating the COX2/PGE2 signaling pathway. The study was approved by the Laboratory Animal Ethics Committee of Nantong University, China(approval No. 20181204-001) on December 4, 2018.

关键词： astrocytes COX2 HMGB1 inflammation spinal cord injury

Decreases of voltage-dependent K~+ currents densities in ventricular myocytes of guinea pigs by chronic oxidant stress

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第6期25卷 49-53页

作者： De-li DONG, Yan LIU, Yu-hong ZHOU,Wei-hua song, He WANG, Bao-feng YANG2 Department ofpharmacology, Harbin Medical University, Harbin 150086, China Department of pharmacology Harbin Medical University Harbin 150086

AIM: To determine the changes of delayed rectifier K+ currents (Ik) and inward rectifier K+ currents (Ik1) in the ventricular myocytes of guinea pigs during the gradual apoptotic process by the chronic oxidant stress treatment. METHODS: H2O250 μmol/L (24 h) was used for inducing apoptosis in the cardiomyocytes culture of neonatal rats and to treat the isolated ventricular myocytes of adult guinea pigs in vitro for 24 h. Apoptosis was evaluated by TUNEL methods and voltage-dependent K+ currents were recorded by patch-clamp techniques. RESULTS: H2O2 50 μmol/L (24 h)induced cell apoptosis in the cardiomyocytes culture of neonatal rats. This concentration was used to treat the isolated ventricular myocytes of adult guinea pigs in vitro for 24 h and the voltage-dependent K+ currents densities (Ik, Ik1) were down-regulated. The densities of the delayed rectifier K+ currents (Ik) in 50 μmol/L H2O2 group were 2.52±0.57 pA/pF vs 5.73±1.84 pA/pF in the control group at +50 mV (n=8, P<0.01). The densities of the inward rectifier K+ currents (Ik1) in 50 μmol/L H2O2 group were -13.9± 2.70 pA/pF, 2.52±0.57 pA/pF vs -59.7±11.9 pA/pF, 5.73±1.84 pA/pF in the control group at -120 mV (n=8, P< 0.01) and -40 mV (n=8, P<0.05), respectively. The extent of inward rectifier property of Ik1 was weakened by 50 μmol/L H2O2treatment. CONCLUSION: The densities of Ik, Ik1 in the cardiomyocytes of guinea pigs were down- regulated and the inward rectifier property of Ik1 was weakened during the gradual apoptotic process after 50 μmol/L H2O2 treatment for 24 h.

关键词： hydrogen peroxide potassium apoptosis cardiac myocytes

Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第9期18卷 2011-2018页

作者： Bing-Qiang He Ai-Cheng Li Yu-Xuan Hou Hui Li Xing-Yuan Zhang Hui-Fei Hao hong-hua song Ri-Xin Cai Ying-Jie Wang Yue Zhou Yong-Jun Wang Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-innovation Center of NeuroregenerationMedical School of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

The low intrinsic growth capacity of neurons and an injury-induced inhibitory milieu are major contributo rs to the failure of sensory and motor functional recovery following spinal cord *** shock transcription factor 1(HSF1),a master regulator of the heat shock response,plays neurogenetic and neuroprotective roles in the damaged or diseased central nervous ***,the underlying mechanism has not been fully *** the present study,we used a gecko model of spontaneous nerve regeneration to investigate the potential roles of gecko HSF1(gHSF1) in the regulation of neurite outgrowth and inflammatory inhibition of macrophages following spinal cord ***1 expression in neurons and microglia at the lesion site increased dramatically immediately after tail ***1 ove rexpression in gecko primary neuro ns significantly promoted axonal growth by suppressing the expression of suppressor of cytokine signaling-3,and fa cilitated neuro nal survival via activation of the mitogen-activated extracellular signal-regulated kinase/extracellular regulated protein kinases and phosphatidylinositol 3-kinase/protein kinase B ***,gHSF1 efficiently inhibited the macrophagemediated inflammatory response by inactivating 1kappa B-alpha/NF-kappaB *** findings show that HSF1 plays dual roles in promoting axonal regrowth and inhibiting leukocyte inflammation,and provide new avenues of investigation for promoting spinal co rd injury repair in mammals.

关键词： apoptosis gecko heat shock factor 1 inflammation neuron regeneration spinal cord suppressor of cytokine signaling-3

Synthesis,Structure and Peoperties of Ni(Ⅱ) Compounds Constructed from N-tosyl-L-glutamic Acid

在线全文

学校读者我要写书评

暂无评论

Synthesis,Structure and Peoperties of Ni(Ⅱ) Compounds Const...

中国化学会第六届全国结构化学学术会议

作者： hong-hong Cui,Hui-hua song~* College of Chemistry and Material Sciences,Hebei Normal University, Shijiazhuang,050016

Coordination polymers,which show a variety of useful properties such as porosity,catalysis,and magnetism,etc,are one of current research focuses in chemistry,material and life *** amino acid derivatives ligands containing two or more carboxylate groups offer great potentials for fine control over coordination *** novel metal-organic frameworks[Ni(tsgluO)(bpe)(HO)]have been

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

非盐依赖层析的研究与应用

生物化学与生物物理进展 2005年第1期32卷 86-90页

作者：韩凌胡洪波彭华松张雪洪上海交通大学生命科学技术学院上海200240

在非盐依赖层析操作中,吸附介质的成分、结构、密度等性质得到改进,所以料液离子强度的变化不会明显影响吸附. 与常用的离子交换层析、疏水层析、亲硫层析等方法相比,该类技术能够降低对料液预处理的要求,提高蛋白质的稳定性,同时简化... 详细信息

在非盐依赖层析操作中,吸附介质的成分、结构、密度等性质得到改进,所以料液离子强度的变化不会明显影响吸附. 与常用的离子交换层析、疏水层析、亲硫层析等方法相比,该类技术能够降低对料液预处理的要求,提高蛋白质的稳定性,同时简化层析操作、降低纯化成本,具有大规模分离纯化蛋白质的潜力. 近年来开发的多种非盐依赖层析介质与方法,都已在蛋白质纯化中得到应用.

关键词：层析非盐依赖混合模式疏水电荷诱导配基

Universal design of three-dimensional porous graphene-iron based promotors for kinetically rationalized lithium-sulfur chemistry

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 192-200页

作者： hua Gao Yunfeng Zhang Menglei Wang Ruoxuan Yang Shuai Feng Xuan Cao Yaping Zhang Zhongyuan Lu Yingze song State Key Laboratory of Environment-Friendly Energy Materials School of Materials and Chemistry Southwest University of Science and Technology School of Material Science and Engineering Henan University of Technology College of Chemistry and Chemical Engineering Taishan University

Lithium-sulfur （Li-S） batteries are widely deemed to be one of the most potential candidates for future secondary batteries because of their remarkable energy ***,notorious polysulfide shuttling and retarded sulfur reaction kinetics pose significant obstacles to the further application of Li-S *** rationally designed highly active electrocatalysts can facilitate polysulfide conversion,the universal and scalable synthesis strategies need to be ***,a universal synthetic strategy to construct a series of three-dimensional （3D） porous graphene-iron （3DGr-Fe） based electrocatalysts involving 3DGr-FeP,3DGr-Fe3C,and 3DGr-Fe3Se4is exploited for manipulating the Li-S redox *** has been observed that the implementation of a 3D porous Gr architecture leads to the well-designed conductive networks,while the uniformly dispersed iron nanoparticles introduce an abundance of active sites,fostering the lithium polysulfide conversion,thereby bolstering the overall electrochemical *** Li-S battery with the 3DGr-Fe based electrocatalyst exhibits remarkable capacity retention of 94.8%upon 100 times at 0.2 ***,the soft-packaged Li-S pouch cell based on such a 3DGr-Fe electrocatalyst delivers superior capacity of 1060.71 mA h g-1and guarantees for the continuous 30 min work of fan *** investigation gives comprehensive insights into the design,synthesis,and mechanism of 3DGr-Fe based electrocatalysts with high activity toward efficient and durable Li-S batteries.

关键词： Lithium-sulfur battery Universal synthesis 3D graphene-iron based promotor Electrocatalysis

Infiltration by monocytes of the central nervous system and its role in multiple sclerosis: reflections on therapeutic strategies

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 779-793页

作者： Guangyong Zhang Qing Yao Chubing Long Pengcheng Yi Jiali song Luojia Wu Wei Wan Xiuqin Rao Yue Lin Gen Wei Jun Ying Fuzhou hua Department of Anesthesiology the Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi ProvinceChina Key Laboratory of Anesthesiology of Jiangxi Province NanchangJiangxi ProvinceChina

Mononuclear macrophage infiltration in the central nervous system is a prominent feature of neuroinflammation. Recent studies on the pathogenesis and progression of multiple sclerosis have highlighted the multiple roles of mononuclear macrophages in the neuroinflammatory process. Monocytes play a significant role in neuroinflammation, and managing neuroinflammation by manipulating peripheral monocytes stands out as an effective strategy for the treatment of multiple sclerosis, leading to improved patient outcomes. This review outlines the steps involved in the entry of myeloid monocytes into the central nervous system that are targets for effective intervention: the activation of bone marrow hematopoiesis, migration of monocytes in the blood, and penetration of the blood–brain barrier by monocytes. Finally, we summarize the different monocyte subpopulations and their effects on the central nervous system based on phenotypic differences. As activated microglia resemble monocyte-derived macrophages, it is important to accurately identify the role of monocyte-derived macrophages in disease. Depending on the roles played by monocyte-derived macrophages at different stages of the disease, several of these processes can be interrupted to limit neuroinflammation and improve patient prognosis. Here, we discuss possible strategies to target monocytes in neurological diseases, focusing on three key aspects of monocyte infiltration into the central nervous system, to provide new ideas for the treatment of neurodegenerative diseases.

关键词： blood–brain barrier macrophages monocytes multiple sclerosis neuroinflammation review therapy

Metabolic pathway modulation by olanzapine:Multitarget approach for treating violent aggression in patients with schizophrenia

在线全文

学校读者我要写书评

暂无评论

World Journal of Psychiatry 2025年第1期15卷 37-53页

作者： Yan-Ning song Shuang Xia Zhi Sun Yong-Chao Chen Lu Jiao Wen-hua Wan hong-Wei Zhang Xiao Guo hua Guo Shou-Feng Jia Xiao-Xin Li Shi-Xian Cao Li-Bin Fu Meng-Meng Liu Tian Zhou Lv-Feng Zhang Qing-Quan Jia Department of Pharmacy The Affiliated Encephalopathy Hospital of Zhengzhou University(Zhumadian Second People's Hospital)Zhumadian 463000Henan ProvinceChina Department of Pharmacy The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of Pharmacy Zhumadian First People's HospitalZhumadian 463000Henan ProvinceChina Scientific Education Section The Affiliated Encephalopathy Hospital of Zhengzhou University(Zhumadian Second People's Hospital)Zhumadian 463000Henan ProvinceChina Department of Psychiatry The Affiliated Encephalopathy Hospital of Zhengzhou University(Zhumadian Second People's Hospital)Zhumadian 463000Henan ProvinceChina Clinical Laboratory The Affiliated Encephalopathy Hospital of Zhengzhou University(Zhumadian Second People's Hospital)Zhumadian 463000Henan ProvinceChina Publicity Division The Affiliated Encephalopathy Hospital of Zhengzhou University(Zhumadian Second People's Hospital)Zhumadian 463000Henan ProvinceChina

BACKGROUND The use of network pharmacology and blood metabolomics to study the patho-genesis of violent aggression in patients with schizophrenia and the related drug mechanisms of action provides new directions for reducing the risk of violent aggression and optimizing treatment *** To explore the metabolic regulatory mechanism of olanzapine in treating patients with schizophrenia with a moderate to high risk of violent *** Metabolomic technology was used to screen differentially abundant metabolites in patients with schizophrenia with a moderate to high risk of violent aggression before and after olanzapine treatment,and the related metabolic pathways were *** pharmacology was used to establish protein-protein interaction networks of the core targets of *** Ontology functional analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were subsequently *** Compared with the healthy group,the patients with schizophrenia group presented significant changes in the levels of 24 metabolites related to the disruption of 9 metabolic pathways,among which the key pathways were the alanine,aspartate and glutamate metabolism and arginine biosynthesis *** treatment with olanzapine,the levels of 10 differentially abundant metabolites were significantly reversed in patients with *** effectively regulated six metabolic pathways,among which the key pathways were alanine,aspartate and glutamate metabolism and arginine biosynthesis *** core targets of olanzapine were involved in several key *** The metabolic pathways of alanine,aspartate,and glutamate metabolism and arginine biosynthesis are the key pathways involved in olanzapine treatment for aggressive schizophrenia.

关键词： Schizophrenia Violent aggression Olanzapine Metabolomics Network pharmacology

Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2020年第5期16卷 502-513页

作者： hong-Wei Li hua-song Zeng Department of Pediatric Allergy Immunology and Rheumatology Guangzhou Women and Children Medical CenterGuangzhou 510623China Department of Pediatric Allergy Immunology and RheumatologyGuangzhou Women and Children's Medical CenterGuangzhou Medical University9 Jinsui RoadGuangzhou 510175China

Background Overexpression of the components of the Janus kinase/signal transducer and activator of transcription(JAK/STAT)signalling pathway is the key factor of the pathogenic mechanisms underlying systemic juvenile idiopathic arthritis(sJIA).The study aims to investigate the association between miR-21 and the JAK/STAT signal pathway in *** Total RNA was extracted from peripheral blood mononuclear cells(PBMCs)in active JIA *** relative expressions of miR-21,STAT3 and suppressor of cytokine signalling 3 in PBMCs were measured by real-time polymerase chain reaction and their expressions were measured by western blotting and dual-luciferase reported *** arthritis fibroblast-like synovial cell(RASF)was stimulated to become to osteoclasts using macrophage colony-stimulating factor(M-CSF)and factors that can impact on their differentiation ability were identified through the transfection of *** expression of STAT3/p-STAT3 was measured by western blot,and the levels of interleukin(IL)-17A,p65,matrix metalloproteinases(MMP)-3,MMP-4 and receptor activator of nuclear factor-κd3 after the LV3-miR-21 transfection were tested by enzyme-linked immunosorbent ***,the miR-21 targeted STAT3 gene was detected by the dualluciferase reported *** The expression of miR-21 was significantly lower in JIA patients than in healthy control(P<0.05).The level of STAT3 was increased in PBMCs of JIA group compared with control group(P<0.05).Furthermore,the expression levels of miR-21 in sJIA and polyarticular JIA groups were negatively correlated with STAT3(r=-0.5854/r=-0.6134,P<0.05).The expression of STAT3 changed little in PBMCS after the stimulation of IL-6 and not in RASFs with transfection of *** expression of p-STAT3 decreased after the stimulation of IL-6 in RASFs transfected by LV3-miR-21(P<0.05).RASFs were induced into osteoclasts using *** number of osteoclasts as determined by tartrate-resistant acid phospha

关键词： JAK/STAT Juvenile idiopathic arthritis MiR-21 Signal transducer and activator of transcription 3 (STAT3)/p-STAT3